FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_35010-1.342000000357e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_35010-1.342000000357e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6764852
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT94820.14016566807374353Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC14800.054.4390959
GATCGGG28050.052.573091
AGAGCGG15000.052.44948
CGGGAGA20950.050.687054
GAGAGCG19050.046.7734537
ATCGGGA31250.041.57142
TTGTGTT541800.039.0592461
AAGCGGC7250.037.9152269
GGCGCCG14700.037.00246456-57
CGCCGGA9650.036.4258658-59
AGCGGCG13900.036.14221610-11
TCGGGAA9600.035.5544783
TGTGTTT965350.035.4738771
CCGGATC9200.035.36636460-61
TCGGGAG32500.035.0074843
GGGCGCC20650.034.96826654-55
CGCCGTA17300.034.733658-59
GTCGCCG19050.034.28863556-57
GATCCGG5600.033.869431
CGGCGTG6350.033.5864512-13
Produced by FastQC (version 0.11.2)