FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_35008-2.342000000358b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_35008-2.342000000358b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694438
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT109060.19152021674483066Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA44800.083.861542
GAGCGGC31250.081.7751549
GATCGGG46900.081.528311
AAGCGGC10250.071.695269
AGAGCGG37150.071.468038
TCGGGAG46500.069.6613163
CGGGAGA45300.068.051724
GAGAGCG41900.066.422377
GATCCGG9350.062.9153021
GAGCGTC94650.058.606789
TCGGGAA14900.057.930743
GTTCGGA5200.057.4715462
AGAGCGT99000.056.4626468
AAGAGCG104150.053.4895367
TCGGAAG113450.052.422413
CGGAAGA114100.050.9599274
GATCGGA124900.050.8585971
ATCGGAA124950.050.6448442
AAAGCGG15650.049.986448
GGTTCGG7500.048.7052421
Produced by FastQC (version 0.11.2)