##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2356005 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.886841072069032 33.0 33.0 33.0 27.0 33.0 2 31.8146188144762 33.0 33.0 33.0 33.0 33.0 3 31.761341338409725 33.0 33.0 33.0 33.0 33.0 4 35.46667642895495 37.0 37.0 37.0 33.0 37.0 5 35.4884238361124 37.0 37.0 37.0 33.0 37.0 6 35.52001587432964 37.0 37.0 37.0 33.0 37.0 7 35.396619277123776 37.0 37.0 37.0 33.0 37.0 8 35.40574829000787 37.0 37.0 37.0 33.0 37.0 9 35.29091449296585 37.0 37.0 37.0 33.0 37.0 10-11 35.215168898198435 37.0 37.0 37.0 33.0 37.0 12-13 35.18291663218032 37.0 37.0 37.0 33.0 37.0 14-15 36.84297529079947 40.0 37.0 40.0 33.0 40.0 16-17 36.80913325735726 40.0 37.0 40.0 33.0 40.0 18-19 36.848878928525195 40.0 37.0 40.0 33.0 40.0 20-21 36.866846207881565 40.0 37.0 40.0 33.0 40.0 22-23 36.90633508842299 40.0 37.0 40.0 33.0 40.0 24-25 36.630545987805625 40.0 37.0 40.0 33.0 40.0 26-27 36.54250224426518 40.0 37.0 40.0 33.0 40.0 28-29 36.46241794902812 40.0 37.0 40.0 33.0 40.0 30-31 36.500373301414896 40.0 37.0 40.0 33.0 40.0 32-33 36.4176975430867 40.0 37.0 40.0 33.0 40.0 34-35 36.38188968189796 40.0 37.0 40.0 33.0 40.0 36-37 36.29245226559367 40.0 37.0 40.0 33.0 40.0 38-39 36.21513939910993 40.0 37.0 40.0 33.0 40.0 40-41 36.089876719276916 38.5 37.0 40.0 33.0 40.0 42-43 35.97115328702613 37.0 37.0 40.0 27.0 40.0 44-45 35.850387414288164 37.0 37.0 40.0 27.0 40.0 46-47 35.803553472934055 37.0 37.0 40.0 27.0 40.0 48-49 35.69198431242718 37.0 37.0 40.0 27.0 40.0 50-51 34.493738977633754 37.0 35.0 38.5 27.0 38.5 52-53 34.94453089021458 37.0 35.0 38.5 27.0 40.0 54-55 35.74765142688577 37.0 37.0 40.0 27.0 40.0 56-57 35.78488946330759 37.0 37.0 40.0 27.0 40.0 58-59 35.5476817748689 37.0 37.0 40.0 27.0 40.0 60-61 35.304335941562094 37.0 37.0 40.0 27.0 40.0 62-63 35.054868092385206 37.0 37.0 40.0 27.0 40.0 64-65 34.842239299152595 37.0 33.0 40.0 27.0 40.0 66-67 34.5951468693827 37.0 33.0 37.0 27.0 40.0 68-69 34.38784149439411 37.0 33.0 37.0 27.0 40.0 70-71 34.06081693375014 37.0 33.0 37.0 27.0 40.0 72-73 33.82341124063828 37.0 33.0 37.0 27.0 38.5 74-75 33.59874002814085 37.0 33.0 37.0 27.0 37.0 76-77 33.35554126582923 37.0 33.0 37.0 27.0 37.0 78-79 33.1360466128043 37.0 33.0 37.0 27.0 37.0 80-81 32.91640870881004 37.0 33.0 37.0 27.0 37.0 82-83 32.73001351864703 37.0 33.0 37.0 24.5 37.0 84-85 32.56766093450565 37.0 33.0 37.0 22.0 37.0 86-87 32.4105855038508 37.0 33.0 37.0 22.0 37.0 88-89 32.27746142304452 33.0 33.0 37.0 22.0 37.0 90-91 32.13769452951077 33.0 33.0 37.0 22.0 37.0 92-93 31.998116727256523 33.0 33.0 37.0 22.0 37.0 94-95 31.8651571197854 33.0 33.0 37.0 22.0 37.0 96-97 31.699917020549616 33.0 33.0 37.0 22.0 37.0 98-99 31.56408454141651 33.0 33.0 37.0 18.5 37.0 100-101 30.340284719259934 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2949789867609063 2306 2 0.25646812774031247 2306 3 0.3247745899513568 2306 4 0.3260131006826654 2306 5 0.3702947574652029 2306 6 0.33942995998145875 2306 7 0.34593715531345026 2306 8 0.32543689377931173 2306 9 0.4107993719757417 2306 10-11 0.38348904707918763 2306 12-13 0.29082320309300513 2306 14-15 0.6047254785505132 2306 16-17 0.5748479085097529 2306 18-19 0.5157201933864997 2306 20-21 0.5426726273477627 2306 22-23 0.5212551500555946 2306 24-25 0.48026298458295 2306 26-27 0.4954926570656184 2306 28-29 0.4558480220570402 2306 30-31 0.4703505650606772 2306 32-33 0.38176524145746527 2306 34-35 0.2895795128503664 2306 36-37 0.33656005741806183 2306 38-39 0.40111803150114866 2306 40-41 0.3477709670718525 2306 42-43 0.3565706963070525 2306 44-45 0.45891978888574414 2306 46-47 0.3682967310998748 2306 48-49 0.4027873090633918 2306 50-51 0.5287024686918471 2306 52-53 0.3606583131809913 2306 54-55 0.42459498075146485 2306 56-57 0.3567817468986334 2306 58-59 0.4809262557858318 2306 60-61 0.3991055811208142 2306 62-63 0.5170643214034882 2306 64-65 0.30517793504770907 2306 66-67 0.4033161282207445 2306 68-69 0.38547250175086845 2306 70-71 0.49948892493394226 2306 72-73 0.44304699535680214 2306 74-75 0.4493341963807822 2306 76-77 0.4685185867015633 2306 78-79 0.4156060242403896 2306 80-81 0.33090592681765685 2306 82-83 0.3181100886325865 2306 84-85 0.31510596983195427 2306 86-87 0.3932678488464063 2306 88-89 0.3965619828934095 2306 90-91 0.39161262027534605 2306 92-93 0.36596077146948147 2306 94-95 0.408646522991166 2306 96-97 0.41730913508561684 2306 98-99 0.339573718502173 2306 100-101 0.07571877863529508 2307 1 0.12615734290312375 2307 2 0.10595669431361898 2307 3 0.13655422609627266 2307 4 0.1919211018469582 2307 5 0.06191290166660224 2307 6 0.14367910276130402 2307 7 0.29902616731513376 2307 8 0.20808922171025301 2307 9 0.35415188481744053 2307 10-11 0.2882290608720055 2307 12-13 0.318951286700198 2307 14-15 0.48816769303490304 2307 16-17 0.48615717566653416 2307 18-19 0.4714811113941053 2307 20-21 0.3453540747967594 2307 22-23 0.3563371943882814 2307 24-25 0.4513918158044845 2307 26-27 0.4469709466592846 2307 28-29 0.4068102886691207 2307 30-31 0.36438831052637255 2307 32-33 0.3115800770728967 2307 34-35 0.30021595729802897 2307 36-37 0.09674524595818212 2307 38-39 0.27045249772987034 2307 40-41 0.3144517043476114 2307 42-43 0.25896747057462477 2307 44-45 0.25623477849281784 2307 46-47 0.24796811293030885 2307 48-49 0.20010139283726147 2307 50-51 0.16224840107803118 2307 52-53 0.2817834908752701 2307 54-55 0.3471871553188137 2307 56-57 0.3356765881181545 2307 58-59 0.3726912657439527 2307 60-61 0.42491186325524666 2307 62-63 0.24228585801062508 2307 64-65 0.22274077269703696 2307 66-67 0.34650589303021917 2307 68-69 0.23739896741918187 2307 70-71 0.302701039667987 2307 72-73 0.36454009230390483 2307 74-75 0.3118113542868315 2307 76-77 0.3043343559290861 2307 78-79 0.2012162901084693 2307 80-81 0.37741215834621045 2307 82-83 0.2552778743052002 2307 84-85 0.2203129406411719 2307 86-87 0.2330553947268541 2307 88-89 0.3536020883565527 2307 90-91 0.5180897322858513 2307 92-93 0.3671741350455662 2307 94-95 0.36927614349535887 2307 96-97 0.27668562593609636 2307 98-99 0.26213751029580834 2307 100-101 0.2448681831405466 2201 1 0.2647087733661486 2201 2 0.21554916301018778 2201 3 0.1876201331782532 2201 4 0.1998227668659709 2201 5 0.21643365437174822 2201 6 0.28492184196520043 2201 7 0.42696063828108066 2201 8 0.23459836749911034 2201 9 0.2785908161765249 2201 10-11 0.28585910649669444 2201 12-13 0.29858344361488776 2201 14-15 0.4831742986524219 2201 16-17 0.4178888613977705 2201 18-19 0.5809190356247953 2201 20-21 0.531924406020714 2201 22-23 0.5014256922188025 2201 24-25 0.38280064806159686 2201 26-27 0.44607202664435164 2201 28-29 0.364493315516512 2201 30-31 0.31245632753553565 2201 32-33 0.3961728660335666 2201 34-35 0.33414882503549137 2201 36-37 0.21132647542891192 2201 38-39 0.2929501652532167 2201 40-41 0.38264669857877465 2201 42-43 0.4431029769067223 2201 44-45 0.37565363133678176 2201 46-47 0.34407391946844257 2201 48-49 0.42257810009706276 2201 50-51 0.10916837348353425 2201 52-53 0.4046019131207359 2201 54-55 0.4515342431095988 2201 56-57 0.5036188599000013 2201 58-59 0.4810464247353252 2201 60-61 0.5513173797567532 2201 62-63 0.5085714240208787 2201 64-65 0.5109862350998 2201 66-67 0.37420246909348265 2201 68-69 0.3966712128957539 2201 70-71 0.48154278477210255 2201 72-73 0.4540032124612736 2201 74-75 0.4853916371386404 2201 76-77 0.4873340830913264 2201 78-79 0.3911319502600108 2201 80-81 0.4815786716949546 2201 82-83 0.5972757826398905 2201 84-85 0.4511452702531713 2201 86-87 0.48737752917399746 2201 88-89 0.43636650998546855 2201 90-91 0.4329031600466706 2201 92-93 0.5281317874088316 2201 94-95 0.5407770264703124 2201 96-97 0.4740514468602157 2201 98-99 0.32478675677569413 2201 100-101 0.32287511556372905 1101 1 0.1338636608833248 1101 2 0.07311025496462165 1101 3 0.14852063906459279 1101 4 0.09896688942273357 1101 5 0.1956981782232674 1101 6 0.3132269334624169 1101 7 0.3382505358692214 1101 8 0.2575095977700457 1101 9 0.35414149053907096 1101 10-11 0.2785625119205335 1101 12-13 0.24096158691034475 1101 14-15 0.40199496767331055 1101 16-17 0.3366731606374458 1101 18-19 0.4174942689913692 1101 20-21 0.32247133083200197 1101 22-23 0.48330265498084657 1101 24-25 0.27308563622409565 1101 26-27 0.32158333752260404 1101 28-29 0.264740902183604 1101 30-31 0.28096591122029935 1101 32-33 0.23387024322803285 1101 34-35 0.1832718803346296 1101 36-37 0.04304411628202587 1101 38-39 0.16612564548895392 1101 40-41 0.027319379207312977 1101 42-43 -0.001445972241647553 1101 44-45 -0.01285035090685227 1101 46-47 -0.13471309637121465 1101 48-49 -0.17239849087675196 1101 50-51 -0.052094741595645644 1101 52-53 0.08902614608328463 1101 54-55 0.24802898792363237 1101 56-57 0.21084026552028945 1101 58-59 0.03534626067543911 1101 60-61 0.059037422499912395 1101 62-63 0.21906413064267127 1101 64-65 0.1712021726348283 1101 66-67 0.19507931730309025 1101 68-69 0.21138845516576055 1101 70-71 0.3302790090989305 1101 72-73 0.3017646276861612 1101 74-75 0.3339210237981476 1101 76-77 0.32012599326439783 1101 78-79 0.17805303055632038 1101 80-81 0.20147457494837084 1101 82-83 0.14238977468851743 1101 84-85 0.10254524704578927 1101 86-87 0.12938766966279047 1101 88-89 0.21374720287681015 1101 90-91 0.08492637138949277 1101 92-93 0.035482318123108314 1101 94-95 0.062184725037568 1101 96-97 0.20907136488223443 1101 98-99 0.060419517981856075 1101 100-101 0.029225625742693495 2304 1 0.15772227334970879 2304 2 0.1609990600054374 2304 3 0.1373612989070061 2304 4 0.3194834016115706 2304 5 0.21564187835834048 2304 6 0.30972613771344015 2304 7 0.2677850670133779 2304 8 0.3099047726644528 2304 9 0.32558175757271357 2304 10-11 0.2761555018786126 2304 12-13 0.32868558581814966 2304 14-15 0.3590564391115336 2304 16-17 0.37153982327576784 2304 18-19 0.3516304236179124 2304 20-21 0.3322821229418409 2304 22-23 0.34695800730705173 2304 24-25 0.12552622083040887 2304 26-27 0.3584030266648526 2304 28-29 0.18723317980662557 2304 30-31 0.2006389930972574 2304 32-33 0.12386720379858929 2304 34-35 0.13259882162820702 2304 36-37 0.12187449022271579 2304 38-39 0.14082909667249766 2304 40-41 0.1763337681116255 2304 42-43 0.11295310893293475 2304 44-45 0.19648649011281094 2304 46-47 0.26766181566024727 2304 48-49 0.39614938876911054 2304 50-51 0.259429852567834 2304 52-53 0.31148364518259086 2304 54-55 0.36574032027047565 2304 56-57 0.4027850051493189 2304 58-59 0.38371825776231816 2304 60-61 0.3630900859532886 2304 62-63 0.4464650186649166 2304 64-65 0.37275496216319937 2304 66-67 0.3309594183993241 2304 68-69 0.32897257671385916 2304 70-71 0.2431695973625594 2304 72-73 0.26398495133768307 2304 74-75 0.22734211289523643 2304 76-77 0.3389732953278042 2304 78-79 0.32873104139740406 2304 80-81 0.27290911749606295 2304 82-83 0.2502770259402993 2304 84-85 0.19324359344170006 2304 86-87 0.26272639431093125 2304 88-89 0.394043067935236 2304 90-91 0.16317952633088595 2304 92-93 0.36509526563820316 2304 94-95 0.5404443995323938 2304 96-97 0.423449501707438 2304 98-99 0.18362033703767366 2304 100-101 0.23696074650890253 2202 1 0.2225827295371765 2202 2 0.22039527873614873 2202 3 0.19137855283071303 2202 4 0.19388803094069118 2202 5 0.21338172889787188 2202 6 0.3039685368870195 2202 7 0.3923126204731773 2202 8 0.2745367745141465 2202 9 0.34647270827790777 2202 10-11 0.3527963109930212 2202 12-13 0.2526948215673315 2202 14-15 0.4123415573371787 2202 16-17 0.5266473792385966 2202 18-19 0.4236582369259665 2202 20-21 0.38175500220892644 2202 22-23 0.29404516147025106 2202 24-25 0.29868983613621225 2202 26-27 0.29450846353869764 2202 28-29 0.367996560930834 2202 30-31 0.24800063185940502 2202 32-33 0.2635253495639134 2202 34-35 0.13767933216107764 2202 36-37 0.22124523321900114 2202 38-39 0.11074651795969004 2202 40-41 0.23701278870645126 2202 42-43 0.2181409527015532 2202 44-45 0.39018978371587565 2202 46-47 0.3537109344034661 2202 48-49 0.3276766176837569 2202 50-51 0.2924173225792188 2202 52-53 0.27852735696103537 2202 54-55 0.3680488333233498 2202 56-57 0.3704927617786353 2202 58-59 0.3126999283850438 2202 60-61 0.3233729705446109 2202 62-63 0.27125984460901265 2202 64-65 0.21398640952855175 2202 66-67 0.29523834481242517 2202 68-69 0.2713106594368071 2202 70-71 0.3220926165234417 2202 72-73 0.38526673502091313 2202 74-75 0.3455982023593904 2202 76-77 0.3046757659013082 2202 78-79 0.14872578079612708 2202 80-81 0.2929131902196289 2202 82-83 0.3447451218783897 2202 84-85 0.26163883429451573 2202 86-87 0.34636528797254584 2202 88-89 0.4743516615940919 2202 90-91 0.3922088181224339 2202 92-93 0.34531625331609916 2202 94-95 0.35176996499400204 2202 96-97 0.2037767620159947 2202 98-99 0.2287532202502014 2202 100-101 0.44408599760715717 1102 1 -0.10425691487516175 1102 2 -0.13061910931995513 1102 3 -0.039054092203702595 1102 4 -0.04063026015155202 1102 5 0.00634322027908496 1102 6 -0.05836164592356852 1102 7 -0.02564937258363642 1102 8 0.11572735103974452 1102 9 -0.006277477342059967 1102 10-11 0.032100185935327374 1102 12-13 0.12899416136224318 1102 14-15 0.10945090255019352 1102 16-17 0.1256309844801109 1102 18-19 0.1105143362978609 1102 20-21 0.08341605010890873 1102 22-23 0.020283296842393383 1102 24-25 -0.06571176521229205 1102 26-27 -0.03295727201806642 1102 28-29 -0.05000656952489635 1102 30-31 -0.09883429680233036 1102 32-33 -0.09545364026210734 1102 34-35 -0.08955011418973413 1102 36-37 -0.07169819784073184 1102 38-39 -0.0907685423417135 1102 40-41 -0.10881176530394754 1102 42-43 0.01882473653957817 1102 44-45 -0.02947690301780881 1102 46-47 -0.11586695574963812 1102 48-49 -0.14946453376207813 1102 50-51 -0.21168387542210354 1102 52-53 -0.07548353943292341 1102 54-55 0.15928155059904725 1102 56-57 0.13272862957707332 1102 58-59 0.23390224411959082 1102 60-61 0.28066833706879635 1102 62-63 0.20389835724120076 1102 64-65 0.18525444476624386 1102 66-67 0.21079480338405432 1102 68-69 0.16722952845934458 1102 70-71 0.198450150687556 1102 72-73 0.002510328623550606 1102 74-75 -0.015706555531956212 1102 76-77 0.034688006785870584 1102 78-79 0.013916517857921917 1102 80-81 0.1710255856202707 1102 82-83 0.20658968271157363 1102 84-85 0.06279519654443533 1102 86-87 0.06990641533793962 1102 88-89 0.16373871435029486 1102 90-91 0.23968639322327334 1102 92-93 0.1557982632292081 1102 94-95 0.37304703039243137 1102 96-97 0.19843191021096374 1102 98-99 0.17449800784075364 1102 100-101 0.17762752631303513 2305 1 0.12084837864584941 2305 2 0.15115766464492353 2305 3 0.1505415505109653 2305 4 0.20252877171363792 2305 5 0.16454725404842208 2305 6 0.2934948004530895 2305 7 0.16125275440204945 2305 8 0.2843200941763371 2305 9 0.20796250516243475 2305 10-11 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13223.0 12 9914.0 13 7526.0 14 6082.0 15 5314.0 16 5231.0 17 5513.0 18 5962.0 19 6450.0 20 7389.0 21 8195.0 22 8940.0 23 10342.0 24 11747.0 25 13563.0 26 16413.0 27 19279.0 28 23594.0 29 28787.0 30 35403.0 31 46199.0 32 64080.0 33 92406.0 34 140784.0 35 238614.0 36 471766.0 37 807325.0 38 205498.0 39 148.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.0472195599837 24.901629460052675 26.33098933507723 14.72016164488639 2 29.266913272715133 22.895028573445384 35.058526767698886 12.779531386140594 3 38.29312388969351 19.717045338126187 26.157683820419308 15.832146951760995 4 28.080190004688905 18.59330383958598 36.46758599756925 16.85892015815586 5 31.822840960832323 17.610554343671733 34.264417225122855 16.302187470373088 6 24.35216089494155 18.467522275547214 41.150425359964935 16.0298914695463 7 32.12547780283371 18.802970558760503 31.85177975977472 17.219771878631068 8 27.53684833151537 27.76949412394093 27.816660668158715 16.876996876384986 9 30.783171515534974 25.65821650426814 25.349764826310555 18.208847153886325 10-11 24.822181622527776 28.57911194576266 28.747578728110838 17.851127703598724 12-13 25.646573799581358 25.482430695015566 28.750162039250686 20.12083346615239 14-15 24.1181592272351 26.07769207365267 30.067636475509453 19.73651222360278 16-17 23.06308116912708 27.57995062246667 31.146001943063933 18.210966265342314 18-19 22.134069676085957 27.89637521017169 31.24057493882341 18.728980174918938 20-21 22.09007084424209 28.541107420333155 30.584942985553283 18.783878749871473 22-23 22.850359402895787 27.809006188748764 30.493417390868387 18.84721701748706 24-25 22.436337509274342 28.134791359298646 30.509342566455455 18.919528564971557 26-27 22.073814748304045 28.512435574197493 30.349119091835664 19.064630585662794 28-29 21.464937128158667 28.56480804773206 30.152978842997886 19.817275981111386 30-31 21.568365007748813 28.6874639450878 29.914825822456304 19.829345224707087 32-33 20.675890972830476 28.49967912526247 31.523372771304114 19.301057130602935 34-35 20.495655931925548 29.31778651582535 31.065398396069156 19.121159156179946 36-37 20.93988804917016 30.03194459757853 30.6551281849347 18.373039168316613 38-39 21.848658789657886 29.13217680360396 29.7853786618029 19.233785744935258 40-41 21.47754446708701 28.52426551849699 29.46279627866964 20.535393735746364 42-43 21.8179102970478 28.7238450537729 30.36680355502117 19.091441094158128 44-45 20.953238769628463 29.35429023455674 30.822806440382383 18.869664555432408 46-47 21.267621352730345 29.185867306645402 30.597811872491565 18.948699468132688 48-49 21.16256662898443 29.746513825008453 29.80077965602108 19.290139889986037 50-51 20.745677349295395 29.493265817909865 29.81358918304761 19.947467649747125 52-53 21.38831346358889 28.9617552679636 29.776693805725913 19.873237462721598 54-55 21.96458618224003 29.059572262611454 29.340733002487518 19.635108552661 56-57 22.219784886421454 28.958296640189392 28.766729145285257 20.055189328103896 58-59 21.73957300731601 28.646202563319008 29.77711241698814 19.837112012376846 60-61 20.588011133303727 29.098444241299926 29.82427194759155 20.489272677804795 62-63 21.38186567173712 29.189406929959066 29.431993394766998 19.996734003536815 64-65 20.939782789668776 29.146530745204746 29.63334844991196 20.280338015214518 66-67 21.064952879877705 28.84771034555892 29.334679673241432 20.752657101321944 68-69 21.21425288070912 28.733105421573242 30.041791865904937 20.010849831812703 70-71 20.66618512399298 30.309939343079566 29.52993388048673 19.49394165244072 72-73 20.353330354750113 31.285468678792576 28.811747509516316 19.54945345694099 74-75 21.05950515015347 30.56328226996311 28.571170082256426 19.806042497626994 76-77 21.439206483506542 29.632498416904795 29.211113062264864 19.717182037323795 78-79 21.413331250017293 29.529642443789317 29.737342524617155 19.319683781576234 80-81 20.402721027029525 30.396106443906866 29.4314886090577 19.76968392000591 82-83 19.66303619638356 30.15442523989596 29.69171170150558 20.4908268622149 84-85 19.682587341184536 29.53658305464434 30.249153818225565 20.53167578594556 86-87 19.68578427771585 29.67953488322575 31.008047559276093 19.626633279782304 88-89 19.9634229289852 30.266418901163117 30.391763940749232 19.37839422910245 90-91 20.382309910234486 29.637396895927836 29.831675555490257 20.14861763834742 92-93 19.767389626633314 30.56452617292493 29.686010059344813 19.98207414109695 94-95 19.324195473589622 31.057553955303014 29.81935103980817 19.798899531299195 96-97 19.92757070466161 31.513874015851943 29.19410595647303 19.364449323013414 98-99 20.468447945497125 30.414732808175433 28.753033851394505 20.363785394932936 100-101 20.28128584896742 30.27645701700822 28.36828062071356 21.0739765133108 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 493.0 1 436.0 2 297.0 3 222.5 4 221.5 5 211.0 6 282.5 7 423.0 8 576.0 9 744.0 10 891.0 11 997.5 12 994.5 13 935.5 14 861.5 15 761.0 16 693.5 17 672.5 18 689.0 19 748.5 20 839.5 21 1015.5 22 1321.5 23 1716.0 24 2297.0 25 3119.5 26 4343.0 27 6041.0 28 7780.5 29 9658.0 30 11892.0 31 14525.5 32 19912.0 33 30120.0 34 45529.0 35 68110.0 36 99422.5 37 137958.0 38 177291.5 39 196287.5 40 192629.0 41 172005.0 42 146838.0 43 137876.5 44 130972.5 45 122176.0 46 120848.5 47 108200.5 48 80910.5 49 61098.5 50 49182.0 51 40686.5 52 33898.5 53 26910.5 54 20585.0 55 15058.0 56 11013.5 57 7952.0 58 6041.5 59 4579.5 60 3478.5 61 2651.5 62 2022.0 63 1592.0 64 1236.5 65 977.0 66 734.0 67 563.0 68 437.5 69 334.0 70 274.0 71 210.0 72 168.0 73 154.0 74 131.0 75 93.5 76 70.0 77 58.5 78 53.5 79 42.5 80 29.5 81 28.0 82 21.0 83 15.5 84 13.5 85 5.5 86 7.5 87 12.0 88 7.5 89 5.0 90 6.5 91 7.0 92 4.5 93 2.5 94 2.5 95 2.5 96 1.5 97 2.5 98 3.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17754631250782577 2 0.18446480376739438 3 0.15339526019681624 4 0.1545837126831225 5 0.16281799062395877 6 0.15967708048157794 7 0.1293290973491143 8 0.11213898102932719 9 0.111969202102712 10-11 0.0903436113251033 12-13 0.13497424665906907 14-15 0.11413388341705558 16-17 0.10634527515858412 18-19 0.10916784981356152 20-21 0.10148535338422456 22-23 0.10097601660437902 24-25 0.4594642201523342 26-27 0.3047107285425965 28-29 0.3992351459355986 30-31 0.26699858446819935 32-33 0.29530922048128083 34-35 0.3140910142380852 36-37 0.29448154821403183 38-39 0.3409373070091108 40-41 0.3361622746980588 42-43 0.3802411285205252 44-45 0.3332972553114276 46-47 0.357681753646533 48-49 0.3524186069214624 50-51 0.36882349570565426 52-53 0.35933709818103104 54-55 0.3345493748952146 56-57 0.35095426367940646 58-59 0.34263509627526256 60-61 0.3130723406783941 62-63 0.32105195022930766 64-65 0.32215551325230635 66-67 0.33575904974734777 68-69 0.32179473303324907 70-71 0.309995097633494 72-73 0.2976861254538934 74-75 0.3052412876882689 76-77 0.29573366779781873 78-79 0.30498661929834614 80-81 0.3143669049938349 82-83 0.3398974110835928 84-85 0.33775395213507614 86-87 0.3301139004373929 88-89 0.3432081001525888 90-91 0.3324059159466979 92-93 0.31589491533337155 94-95 0.3089339793421491 96-97 0.29522433101797324 98-99 0.31854771106173374 100-101 0.32304685261703603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2356005.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.32507136967572 #Duplication Level Percentage of deduplicated Percentage of total 1 89.08457210785929 38.59595444510027 2 4.383283685841366 3.7981215704522486 3 1.824176409949242 2.370977194557892 4 1.0342506959785023 1.7923594086962158 5 0.665227112638494 1.441050606605303 6 0.42267033783983127 1.0987333531653383 7 0.32846683084294176 0.9961594222189158 8 0.2213096904254496 0.7670606505986755 9 0.20792980468559763 0.8107716265097625 >10 1.3985855897143138 12.35886276792825 >50 0.21090713280629636 6.411460331932006 >100 0.18824324557697286 17.707023124763722 >500 0.02407426208640886 7.14401139550932 >1k 0.006203527321782864 4.4739091471964345 >5k 9.95664336484929E-5 0.23354495476577028 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5414 0.22979577717364774 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4643 0.1970708890685716 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4062 0.1724104999777165 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3657 0.15522038365792942 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3534 0.1499996816645126 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3451 0.1464767689372476 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2896 0.1229199428693912 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2867 0.12168904565143114 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2702 0.1146856649285549 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2657 0.11277565200413411 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2534 0.1075549500107173 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.762288280372919E-4 0.0 0.0 2 9.762288280372919E-4 0.0 0.0 3 0.0010186735596910872 0.0 0.0 4 0.0011460077546524732 0.0 0.0 5 0.001273341949613859 0.0 0.0 6 0.0013582314129214497 0.0 0.0 7 0.001570455071190426 0.0 0.0 8 0.0019100129244207886 0.0 0.0 9 0.0020797918510359697 0.0 4.2444731653795305E-5 10-11 0.0030984654107270573 0.0 4.2444731653795305E-5 12-13 0.0041171389704181445 0.0 4.2444731653795305E-5 14-15 0.0057088164074354675 0.0 6.366709748069295E-5 16-17 0.00713071491783761 0.0 8.488946330759061E-5 18-19 0.008977060744777707 0.0 8.488946330759061E-5 20-21 0.010717294742583313 0.0 8.488946330759061E-5 22-23 0.012945643154407566 0.0 8.488946330759061E-5 24-25 0.015810662541038748 0.0 8.488946330759061E-5 26-27 0.01890912795176581 0.0 8.488946330759061E-5 28-29 0.02219859465493494 0.0 8.488946330759061E-5 30-31 0.03516546017516941 0.0 8.488946330759061E-5 32-33 0.03921893204810686 0.0 8.488946330759061E-5 34-35 0.04259328821458358 0.0 8.488946330759061E-5 36-37 0.04690142847744381 0.0 1.0611182913448825E-4 38-39 0.05150668186188059 0.0 1.273341949613859E-4 40-41 0.05628171417293257 0.0 1.273341949613859E-4 42-43 0.062266421336117705 0.0 1.273341949613859E-4 44-45 0.06899391130324427 0.0 1.273341949613859E-4 46-47 0.07559406707540943 0.0 1.273341949613859E-4 48-49 0.08242766887167048 0.0 1.273341949613859E-4 50-51 0.08983427454525775 0.0 1.273341949613859E-4 52-53 0.09840811033932441 0.0 1.273341949613859E-4 54-55 0.10713050269417934 0.0 1.273341949613859E-4 56-57 0.11672301204793709 0.0 1.273341949613859E-4 58-59 0.12620940957256033 0.0 1.273341949613859E-4 60-61 0.13758459765577746 0.0 1.273341949613859E-4 62-63 0.15063635263931952 0.0 1.273341949613859E-4 64-65 0.16347588396459262 0.0 1.273341949613859E-4 66-67 0.17748264541034506 0.0 1.273341949613859E-4 68-69 0.19172285288019336 0.0 1.273341949613859E-4 70-71 0.20802162983525077 0.0 1.4855656078828356E-4 72-73 0.22586963949567168 0.0 1.6977892661518122E-4 74-75 0.24617944359201274 0.0 1.6977892661518122E-4 76-77 0.2693118223433312 0.0 1.6977892661518122E-4 78-79 0.2926139800212648 0.0 1.6977892661518122E-4 80-81 0.3180383742818882 0.0 1.6977892661518122E-4 82-83 0.34611556427087375 0.0 1.6977892661518122E-4 84-85 0.37816133666948926 0.0 1.6977892661518122E-4 86-87 0.41496091901332977 0.0 1.6977892661518122E-4 88-89 0.45441329708553246 0.0 1.6977892661518122E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 805 0.0 65.98631 4 GAGCGGC 535 0.0 65.58832 9 ATTCGGA 125 0.0 60.71773 2 AGAGCGG 600 0.0 58.482925 8 CGGAAAG 305 0.0 57.532745 5 GATCCGG 225 0.0 56.9265 1 TCGGGAA 210 0.0 51.94458 3 ATCGCGT 55 8.2920087E-7 51.72937 8 GAGAGCG 745 0.0 51.556873 7 TTGTGTT 29355 0.0 51.018272 1 TCGGGAG 1130 0.0 48.6868 3 TCGCGTT 50 2.856721E-5 47.418587 9 GGATCGG 395 0.0 46.838257 1 TTCGGAG 175 0.0 46.07258 3 TCGGAGA 230 0.0 45.365585 4 TCGGAAA 305 0.0 41.985138 4 ACCGGAA 80 2.291199E-7 41.499203 3 ATCGGGA 1530 0.0 40.304863 2 AAGCGGC 250 0.0 39.831615 9 GCGCACC 85 3.681762E-7 39.064713 2 >>END_MODULE