FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356005
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54140.22979577717364774No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG46430.1970708890685716No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC40620.1724104999777165No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA36570.15522038365792942No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG35340.1499996816645126No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA34510.1464767689372476No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC28960.1229199428693912No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28670.12168904565143114No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT27020.1146856649285549No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA26570.11277565200413411No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG25340.1075549500107173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA8050.065.986314
GAGCGGC5350.065.588329
ATTCGGA1250.060.717732
AGAGCGG6000.058.4829258
CGGAAAG3050.057.5327455
GATCCGG2250.056.92651
TCGGGAA2100.051.944583
ATCGCGT558.2920087E-751.729378
GAGAGCG7450.051.5568737
TTGTGTT293550.051.0182721
TCGGGAG11300.048.68683
TCGCGTT502.856721E-547.4185879
GGATCGG3950.046.8382571
TTCGGAG1750.046.072583
TCGGAGA2300.045.3655854
TCGGAAA3050.041.9851384
ACCGGAA802.291199E-741.4992033
ATCGGGA15300.040.3048632
AAGCGGC2500.039.8316159
GCGCACC853.681762E-739.0647132
Produced by FastQC (version 0.11.2)