##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_35005-1.3420000003578b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583735 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10832805995971 33.0 33.0 33.0 33.0 33.0 2 32.059348578704856 33.0 33.0 33.0 33.0 33.0 3 32.03314310484628 33.0 33.0 33.0 33.0 33.0 4 35.79522358136574 37.0 37.0 37.0 33.0 37.0 5 35.81666192546836 37.0 37.0 37.0 33.0 37.0 6 35.817234739630806 37.0 37.0 37.0 33.0 37.0 7 35.73768981726067 37.0 37.0 37.0 33.0 37.0 8 35.702316220510234 37.0 37.0 37.0 33.0 37.0 9 35.635938670180956 37.0 37.0 37.0 33.0 37.0 10-11 35.5614267717084 37.0 37.0 37.0 33.0 37.0 12-13 35.53590944891794 37.0 37.0 37.0 33.0 37.0 14-15 37.28146152759474 40.0 37.0 40.0 33.0 40.0 16-17 37.27258426270496 40.0 37.0 40.0 33.0 40.0 18-19 37.292283844898954 40.0 37.0 40.0 33.0 40.0 20-21 37.291542863335444 40.0 37.0 40.0 33.0 40.0 22-23 37.27985300350075 40.0 37.0 40.0 33.0 40.0 24-25 37.061291502418015 40.0 37.0 40.0 33.0 40.0 26-27 36.97829131083489 40.0 37.0 40.0 33.0 40.0 28-29 36.90502354150097 40.0 37.0 40.0 33.0 40.0 30-31 36.93920738775455 40.0 37.0 40.0 33.0 40.0 32-33 36.84419396726057 40.0 37.0 40.0 33.0 40.0 34-35 36.83429415168351 40.0 37.0 40.0 33.0 40.0 36-37 36.738621801384426 40.0 37.0 40.0 33.0 40.0 38-39 36.66313224846975 40.0 37.0 40.0 33.0 40.0 40-41 36.51077916272373 40.0 37.0 40.0 33.0 40.0 42-43 36.438574582919685 40.0 37.0 40.0 33.0 40.0 44-45 36.32996263161664 40.0 37.0 40.0 33.0 40.0 46-47 36.26496409267978 38.5 37.0 40.0 33.0 40.0 48-49 36.15101335856812 37.0 37.0 40.0 33.0 40.0 50-51 34.93263395820392 37.0 35.0 38.5 27.0 38.5 52-53 35.36790170044529 37.0 37.0 38.5 30.0 40.0 54-55 36.17722289631096 37.0 37.0 40.0 33.0 40.0 56-57 36.20264694328172 37.0 37.0 40.0 33.0 40.0 58-59 35.98758851043161 37.0 37.0 40.0 30.0 40.0 60-61 35.75843381770963 37.0 37.0 40.0 27.0 40.0 62-63 35.478086374957186 37.0 37.0 40.0 27.0 40.0 64-65 35.2650962656774 37.0 37.0 40.0 27.0 40.0 66-67 35.0130396499641 37.0 35.0 37.0 27.0 40.0 68-69 34.78679218263483 37.0 33.0 37.0 27.0 40.0 70-71 34.46146237907526 37.0 33.0 37.0 27.0 40.0 72-73 34.22226873111987 37.0 33.0 37.0 27.0 38.5 74-75 33.99482125682394 37.0 33.0 37.0 27.0 37.0 76-77 33.76208183114754 37.0 33.0 37.0 27.0 37.0 78-79 33.545214776283174 37.0 33.0 37.0 27.0 37.0 80-81 33.314372797519866 37.0 33.0 37.0 27.0 37.0 82-83 33.122056828583425 37.0 33.0 37.0 27.0 37.0 84-85 32.97062372882668 37.0 33.0 37.0 27.0 37.0 86-87 32.82036431754804 37.0 33.0 37.0 27.0 37.0 88-89 32.67692797442462 37.0 33.0 37.0 27.0 37.0 90-91 32.546482901690766 35.0 33.0 37.0 22.0 37.0 92-93 32.439994620191314 33.0 33.0 37.0 22.0 37.0 94-95 32.30499586838434 33.0 33.0 37.0 22.0 37.0 96-97 32.13672474150793 33.0 33.0 37.0 22.0 37.0 98-99 32.00013662391848 33.0 33.0 37.0 22.0 37.0 100-101 30.74216783067923 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3202723355238888 2306 2 0.3595660714564346 2306 3 0.2904432023726784 2306 4 0.4170380258108608 2306 5 0.4068731981761857 2306 6 0.3358210325822668 2306 7 0.4892573080478968 2306 8 0.3418969756014292 2306 9 0.3809757416344084 2306 10-11 0.4125764504731677 2306 12-13 0.40598631459458545 2306 14-15 0.6731237494430289 2306 16-17 0.5920829058351771 2306 18-19 0.7436130307039122 2306 20-21 0.6819290451338915 2306 22-23 0.4972654514139663 2306 24-25 0.5285994805408478 2306 26-27 0.5257125830963361 2306 28-29 0.5551727619764719 2306 30-31 0.43901438237213597 2306 32-33 0.508018277702547 2306 34-35 0.35409614424066405 2306 36-37 0.4344079305253459 2306 38-39 0.4741860177733983 2306 40-41 0.5110517542384656 2306 42-43 0.3658827235972595 2306 44-45 0.3863378720026631 2306 46-47 0.30506238175316724 2306 48-49 0.4610622958789534 2306 50-51 0.31996489243819326 2306 52-53 0.4639721462362303 2306 54-55 0.4531614489142797 2306 56-57 0.37186197046883507 2306 58-59 0.45535018218224366 2306 60-61 0.5199315081036247 2306 62-63 0.5162373846007142 2306 64-65 0.46459437899498823 2306 66-67 0.3902090750402394 2306 68-69 0.36285622757436187 2306 70-71 0.4492147011797627 2306 72-73 0.469200316747056 2306 74-75 0.3005837042163364 2306 76-77 0.2672537933158523 2306 78-79 0.3833349760342486 2306 80-81 0.44660559776061604 2306 82-83 0.43714727536301723 2306 84-85 0.18027982242305995 2306 86-87 0.4549401264262727 2306 88-89 0.3479276727187326 2306 90-91 0.42379058963540217 2306 92-93 0.3469495759853487 2306 94-95 0.34590463048835574 2306 96-97 0.3967114956676099 2306 98-99 0.2152697696012993 2306 100-101 0.30040752604628196 2307 1 0.21635166811942952 2307 2 0.1797271195012371 2307 3 0.1950096539491355 2307 4 0.23213037423132477 2307 5 0.26570734590797684 2307 6 0.20039944978195479 2307 7 0.3716922353426142 2307 8 0.2622100332803541 2307 9 0.39691646814802084 2307 10-11 0.4368535701987284 2307 12-13 0.39205856025448327 2307 14-15 0.6755470449194618 2307 16-17 0.5455561462337855 2307 18-19 0.47905875016910926 2307 20-21 0.5140186127024364 2307 22-23 0.5600473561049597 2307 24-25 0.436086853727069 2307 26-27 0.40903722894213956 2307 28-29 0.45782856543212347 2307 30-31 0.44778459361931766 2307 32-33 0.46058973121763813 2307 34-35 0.36106188301518927 2307 36-37 0.3528295980607581 2307 38-39 0.4221754849436863 2307 40-41 0.4556248562364118 2307 42-43 0.4204404448906729 2307 44-45 0.37674252062161173 2307 46-47 0.2789670521978209 2307 48-49 0.28533196918662895 2307 50-51 0.34027011853969213 2307 52-53 0.3877131238738656 2307 54-55 0.3383018652460734 2307 56-57 0.40467563798370776 2307 58-59 0.47958403565214525 2307 60-61 0.44149068530012414 2307 62-63 0.35588968823995515 2307 64-65 0.3121858479678181 2307 66-67 0.3170272206767706 2307 68-69 0.3589982127893734 2307 70-71 0.27786273981897125 2307 72-73 0.40244831584321616 2307 74-75 0.3527223667883277 2307 76-77 0.4539374155616329 2307 78-79 0.4210259188791454 2307 80-81 0.43140116791229133 2307 82-83 0.2524449138214564 2307 84-85 0.24106326261676259 2307 86-87 0.3386229706497872 2307 88-89 0.4176454021261762 2307 90-91 0.3974773881635798 2307 92-93 0.29479730444272434 2307 94-95 0.36306913726588164 2307 96-97 0.3429717248632187 2307 98-99 0.3443183741682603 2307 100-101 0.18015057327454187 2201 1 0.21409958011798125 2201 2 0.18386734240370828 2201 3 0.17449359260993447 2201 4 0.2906639235511861 2201 5 0.24726470054095984 2201 6 0.3092722480594077 2201 7 0.3307421559429571 2201 8 0.3764976879547248 2201 9 0.34237728701287296 2201 10-11 0.3551006335051454 2201 12-13 0.32614897832879564 2201 14-15 0.5810919854927832 2201 16-17 0.5390240199090215 2201 18-19 0.5354341054620804 2201 20-21 0.46117032075233766 2201 22-23 0.39673872843376046 2201 24-25 0.3540142900933887 2201 26-27 0.41441516586362326 2201 28-29 0.21131802560557844 2201 30-31 0.20689230915156287 2201 32-33 0.21660179431438564 2201 34-35 0.1928675780949547 2201 36-37 0.18078826101056222 2201 38-39 0.18898900148270315 2201 40-41 0.22963789838296123 2201 42-43 0.16698482756969923 2201 44-45 0.012338524020023556 2201 46-47 0.021328025304036657 2201 48-49 0.047208873274392715 2201 50-51 0.1728469064906335 2201 52-53 0.13211355615079157 2201 54-55 0.28368656618008714 2201 56-57 0.34435559626925993 2201 58-59 0.39634577376634894 2201 60-61 0.26952740331373093 2201 62-63 0.30792513640592745 2201 64-65 0.2423469627354251 2201 66-67 0.2836083724185485 2201 68-69 0.28050740801772633 2201 70-71 0.31249468911258305 2201 72-73 0.35512768979830156 2201 74-75 0.2622930115208817 2201 76-77 0.26101583617382573 2201 78-79 0.22736196176771983 2201 80-81 0.2172626268970319 2201 82-83 0.1970365297276402 2201 84-85 0.28221081563039263 2201 86-87 0.2537956899833702 2201 88-89 0.18692682606933175 2201 90-91 0.22584444432311557 2201 92-93 0.22154644435569537 2201 94-95 0.31943248233444166 2201 96-97 0.21038853297857685 2201 98-99 0.13105564515355894 2201 100-101 0.026815445624318812 1101 1 0.07029848997179045 1101 2 -0.031951404898528324 1101 3 0.004996856690993923 1101 4 0.1389648781819588 1101 5 0.14952002830422373 1101 6 0.1910116301760567 1101 7 0.2575010957774566 1101 8 0.27674412523786174 1101 9 0.2760558779202853 1101 10-11 0.31197159941214636 1101 12-13 0.24948092583581172 1101 14-15 0.4258122381871985 1101 16-17 0.2642065056297085 1101 18-19 0.2329917329720459 1101 20-21 0.2726192398078098 1101 22-23 0.36516970257206793 1101 24-25 0.2050604774258744 1101 26-27 0.24616441885583384 1101 28-29 0.28823410475996525 1101 30-31 0.30824779786028955 1101 32-33 0.21422253687191528 1101 34-35 0.24877124002249218 1101 36-37 0.05218706445967314 1101 38-39 0.1965433582194933 1101 40-41 0.27507006706451165 1101 42-43 0.15381515558391357 1101 44-45 0.08941019494176317 1101 46-47 0.11233143326565198 1101 48-49 0.056784040828326 1101 50-51 0.04683305045818997 1101 52-53 0.06508382808504365 1101 54-55 0.25934732928242 1101 56-57 0.23838003588794265 1101 58-59 0.11068828143719855 1101 60-61 0.14167551496504416 1101 62-63 0.34943091337767385 1101 64-65 0.18959968057226462 1101 66-67 0.10472196611992501 1101 68-69 0.2824748910113897 1101 70-71 0.31858430829754525 1101 72-73 0.20592752349914178 1101 74-75 0.33993978642141087 1101 76-77 0.37516916600494454 1101 78-79 0.20341735076387124 1101 80-81 0.13250882159084654 1101 82-83 0.23307024462329196 1101 84-85 0.1627855599078032 1101 86-87 0.06527702399037594 1101 88-89 0.12538630863468114 1101 90-91 0.06573926094232263 1101 92-93 0.20438262295496656 1101 94-95 0.21519624551373084 1101 96-97 0.26222296828084524 1101 98-99 0.21933619718645758 1101 100-101 0.004757175670928149 2304 1 0.24500735201299761 2304 2 0.2326300339237548 2304 3 0.1740516539697765 2304 4 0.32288913149179166 2304 5 0.32576317859606263 2304 6 0.4072653079268349 2304 7 0.4374079872824126 2304 8 0.3718269053063352 2304 9 0.3834015631113914 2304 10-11 0.2899415196330182 2304 12-13 0.3331349009370612 2304 14-15 0.31661555209522874 2304 16-17 0.34531991967613607 2304 18-19 0.32004877487202776 2304 20-21 0.3700183313700407 2304 22-23 0.2992204283831654 2304 24-25 0.23383002462533398 2304 26-27 0.17780221552515485 2304 28-29 0.2640851999596521 2304 30-31 0.20167244517688232 2304 32-33 0.26448410016864443 2304 34-35 0.11465850500488983 2304 36-37 0.10624628102968359 2304 38-39 0.1019840525680138 2304 40-41 0.21611707000438685 2304 42-43 0.263804106389685 2304 44-45 0.2015971006224575 2304 46-47 0.11344968592388938 2304 48-49 0.29512248394699725 2304 50-51 0.08768163939608087 2304 52-53 0.1225699760128478 2304 54-55 0.3374590262400048 2304 56-57 0.43379280387147645 2304 58-59 0.452063262645062 2304 60-61 0.38560493152198916 2304 62-63 0.3124732137433952 2304 64-65 0.20170297334035325 2304 66-67 0.2230922080389277 2304 68-69 0.3172846837124297 2304 70-71 0.29538747380087216 2304 72-73 0.2805860090986698 2304 74-75 0.29542194906446895 2304 76-77 0.36037681085702644 2304 78-79 0.22608752246116381 2304 80-81 0.1705554987360216 2304 82-83 0.17251861195895657 2304 84-85 0.20533394312739972 2304 86-87 0.32421058157971316 2304 88-89 0.31530800582802243 2304 90-91 0.11824824734976858 2304 92-93 0.26638525945733704 2304 94-95 0.2969244376545461 2304 96-97 0.18456305544541607 2304 98-99 0.1810784104803531 2304 100-101 0.09951135911846976 2202 1 0.26397566438068054 2202 2 0.1726632944896238 2202 3 0.1683494373022114 2202 4 0.1643215030637819 2202 5 0.12763438249056946 2202 6 0.22697154587022794 2202 7 0.28912712083349845 2202 8 0.24586984821907976 2202 9 0.30757761745483947 2202 10-11 0.34095358683021715 2202 12-13 0.28285591761008533 2202 14-15 0.3885062771078154 2202 16-17 0.3303034498966255 2202 18-19 0.2887901566805837 2202 20-21 0.19710072141322144 2202 22-23 0.27517222698807586 2202 24-25 0.3069004941413027 2202 26-27 0.26561301799084447 2202 28-29 0.29297333332966247 2202 30-31 0.17085348222055075 2202 32-33 0.018259167300755053 2202 34-35 0.1091986396397786 2202 36-37 0.09069119368301415 2202 38-39 -0.0200929894301467 2202 40-41 0.09609287566507163 2202 42-43 0.05540903244371975 2202 44-45 0.03905785487504687 2202 46-47 0.18130840531230064 2202 48-49 0.1426756225515433 2202 50-51 0.012117868902862483 2202 52-53 0.05815135045066455 2202 54-55 0.196325971384546 2202 56-57 0.15827546409246906 2202 58-59 0.10455002820666692 2202 60-61 0.09694892334677263 2202 62-63 0.28804698688094277 2202 64-65 0.2384229643876381 2202 66-67 0.18532770327356474 2202 68-69 0.24828931632008278 2202 70-71 0.3066345073694947 2202 72-73 0.3012501598520032 2202 74-75 0.25684588223546 2202 76-77 0.32410959908171577 2202 78-79 0.2011589464847816 2202 80-81 0.14515915725637996 2202 82-83 0.03925049090070587 2202 84-85 0.14985331872829022 2202 86-87 0.2116643393018336 2202 88-89 0.1906184824096826 2202 90-91 0.3532194959546757 2202 92-93 0.2793996042069864 2202 94-95 0.40852273429644015 2202 96-97 0.2516163314089752 2202 98-99 0.2503503580821089 2202 100-101 0.39569710609520214 1102 1 0.07937436715821633 1102 2 0.011604815336383467 1102 3 0.0035175640515561213 1102 4 0.08156836025696634 1102 5 0.01728943597056798 1102 6 0.028369160010925043 1102 7 0.03855027892844731 1102 8 0.06724115224092486 1102 9 -0.016417917493299683 1102 10-11 0.06345198660773121 1102 12-13 0.05249520578022526 1102 14-15 0.12906839679859416 1102 16-17 -0.00988166524656009 1102 18-19 -0.025721663702036324 1102 20-21 -0.04716865710105367 1102 22-23 -0.06775607885893464 1102 24-25 -0.02778107790923201 1102 26-27 -0.07842602559598788 1102 28-29 -0.09955190732320318 1102 30-31 -0.09055255035451637 1102 32-33 -0.15684376513728893 1102 34-35 -0.20965341273869598 1102 36-37 -0.18833754535842218 1102 38-39 -0.1528083179290789 1102 40-41 -0.11165668830255981 1102 42-43 -0.20864693172737248 1102 44-45 -0.24135436712449376 1102 46-47 -0.26375787813812934 1102 48-49 -0.20654382215659695 1102 50-51 -0.15670824003080952 1102 52-53 -0.23350470527173428 1102 54-55 -0.06628020712343385 1102 56-57 -0.06642657975537958 1102 58-59 0.04945105718780951 1102 60-61 0.05240497461550575 1102 62-63 0.051243443637595476 1102 64-65 0.1266517562703129 1102 66-67 -0.03385623097825885 1102 68-69 -0.05890367327390322 1102 70-71 -0.09067105785520368 1102 72-73 0.06272701577103135 1102 74-75 -0.014200664105196381 1102 76-77 -0.015263391640765178 1102 78-79 -0.28344961724626927 1102 80-81 -0.12412500960743245 1102 82-83 -0.009772908763792998 1102 84-85 -0.07556507896637754 1102 86-87 0.004911157651001474 1102 88-89 0.0701478905893893 1102 90-91 0.051587562026995215 1102 92-93 0.04425781436546572 1102 94-95 0.20907247748229452 1102 96-97 0.04725214002655065 1102 98-99 0.07966417364036715 1102 100-101 0.03672195504307041 2305 1 0.07077117655934018 2305 2 0.03501020612325334 2305 3 -0.02193826716194991 2305 4 0.07587776451703832 2305 5 -9.413026585960438E-4 2305 6 0.05204734675081113 2305 7 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 931.0 3 159.0 4 149.0 5 250.0 6 504.0 7 1462.0 8 4790.0 9 8645.0 10 11777.0 11 10582.0 12 7740.0 13 6191.0 14 5349.0 15 5237.0 16 5234.0 17 5621.0 18 5942.0 19 6753.0 20 7439.0 21 8522.0 22 9397.0 23 10832.0 24 12178.0 25 14058.0 26 16616.0 27 19796.0 28 24272.0 29 29672.0 30 36928.0 31 48407.0 32 66534.0 33 95746.0 34 145765.0 35 247365.0 36 493929.0 37 921553.0 38 286415.0 39 995.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.63489604173839 25.075646191402313 23.90508826293701 14.384369503922281 2 32.81567650629529 22.220068065326274 32.51257370609381 12.45168172228463 3 39.043966471611576 18.478446257516133 26.03574562831617 16.44184164255612 4 31.698501578662757 17.771963181979405 33.7198101340652 16.80972510529264 5 31.047301304978554 16.5296378453859 35.54424761950544 16.87881323013011 6 25.34731442611372 17.18005147351564 40.559913071086406 16.912721029284235 7 33.80422183650004 17.249725918941763 30.146978752856583 18.799073491701613 8 30.667068595356096 26.486726085468998 24.97321659319715 17.872988725977752 9 29.788795424869523 26.684928959165568 24.836821121232433 18.689454494732477 10-11 24.536477759745324 28.159277524141896 30.16994475793026 17.134299958182524 12-13 25.237480222496313 25.8176913827733 29.736277343337463 19.208551051392927 14-15 23.45826992542164 26.793556755206875 30.82875068897075 18.91942263040073 16-17 22.60338974908046 27.97287844291066 31.569191023873454 17.854540784135427 18-19 22.14754478192543 28.04445801394214 31.484370883301978 18.323626320830453 20-21 22.110263950069154 28.400918963105877 31.03538317894598 18.453433907878985 22-23 22.297552000663266 27.754246234942386 31.0693526847719 18.87884907962245 24-25 22.202683203092647 27.9111874785411 30.972980938860562 18.91314837950569 26-27 21.845028062613967 28.330817008929486 30.9473932263834 18.876761702073143 28-29 21.378429178908934 28.57517293316645 30.417954039666697 19.628443848257923 30-31 21.280981512237176 28.904377340755836 30.31029101097367 19.504350136033317 32-33 20.692157685183584 29.192713676055593 31.373277073669325 18.7418515650915 34-35 21.512721947079232 28.761619698007728 31.18871284526153 18.536945509651513 36-37 20.93870782974037 29.119395131385282 31.514261962348662 18.427635076525686 38-39 21.259007202266417 29.04156784886834 30.815346115225 18.88407883364025 40-41 21.332996757218588 29.010621565467293 29.969708149672808 19.686673527641315 42-43 21.377751574236047 28.77135283770435 30.69617997041417 19.15471561764543 44-45 20.767180433557197 28.839936147585217 31.31191625011335 19.08096716874424 46-47 21.085246897563266 28.744618221989505 30.84071490832138 19.329419972125848 48-49 20.865676621689516 29.69729032042554 29.94222827899234 19.49480477889261 50-51 21.00360681396287 29.214940589253445 29.696865632243274 20.08458696454041 52-53 21.32916686817271 28.832300165730224 29.76460792556765 20.07392504052942 54-55 21.953290128401175 28.957013519745622 29.42978227626884 19.659914075584357 56-57 22.020178087645533 29.209655962634614 29.091228649388732 19.67893730033112 58-59 21.61114892451872 29.208154879402198 29.730801981918827 19.449894214160253 60-61 20.821496775130406 29.516210349673592 29.613821307882006 20.048471567313996 62-63 21.17917707323741 29.28143017346356 29.404367497949753 20.135025255349287 64-65 20.75431291822274 29.21620459313292 29.650238859609185 20.379243629035155 66-67 21.179442199852463 28.37681209512634 29.739612874712932 20.704132830308264 68-69 21.26157078650769 28.719334272904074 30.163327268643002 19.85576767194523 70-71 20.576710878915485 30.31436096980672 29.37064131703374 19.738286834244057 72-73 20.387307957556025 30.541306825162884 28.94914343601154 20.12224178126955 74-75 20.935713972348736 29.734389140447163 29.022045460633773 20.307851426570327 76-77 20.892781512921474 29.39712723259432 29.263780079294 20.44631117519021 78-79 20.69697279110966 29.559672000928668 29.45576007506972 20.28759513289196 80-81 20.040104631582135 29.984220619348456 29.473332108981783 20.502342640087626 82-83 19.488654601648363 29.688920286563764 29.640626295582916 21.181798816204957 84-85 19.64591411279298 29.334458808689384 30.075882204906417 20.943744873611216 86-87 19.98437719349854 29.36660451329405 30.593051132556386 20.05596716065103 88-89 20.18325249387951 29.971548732723345 29.913368405946432 19.931830367450708 90-91 20.43564440257195 29.650801762942557 29.45303577108154 20.460518063403953 92-93 19.879784488817133 30.16057726431571 29.69211689726643 20.26752134960073 94-95 19.48918536855629 30.600074311887067 29.81535398510113 20.09538633445552 96-97 19.851833390980424 31.104293097217063 29.322415711995912 19.7214577998066 98-99 20.512985287114155 30.251081824131475 28.757583058602066 20.4783498301523 100-101 20.475684201574886 30.06161221542647 28.172900954102065 21.289802628896584 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 597.0 1 492.5 2 315.0 3 261.5 4 271.0 5 282.5 6 338.5 7 477.0 8 651.5 9 840.0 10 1055.5 11 1177.0 12 1183.0 13 1130.5 14 980.0 15 838.0 16 773.5 17 691.5 18 666.0 19 708.5 20 801.0 21 975.5 22 1223.5 23 1557.0 24 2181.0 25 3124.5 26 4849.5 27 7544.5 28 10387.5 29 13145.5 30 16314.5 31 19771.5 32 25333.0 33 36019.5 34 52645.0 35 76633.5 36 110230.0 37 147264.0 38 180664.0 39 200001.5 40 198851.0 41 181858.0 42 163304.0 43 155381.0 44 143613.5 45 129175.5 46 121506.5 47 108511.0 48 90418.0 49 75686.0 50 62657.0 51 51242.5 52 41589.5 53 33106.0 54 27043.0 55 21584.0 56 15605.5 57 10458.0 58 7199.0 59 5254.0 60 3952.0 61 2922.0 62 2065.5 63 1499.0 64 1157.5 65 889.5 66 666.0 67 512.0 68 400.5 69 302.5 70 233.5 71 180.0 72 135.0 73 108.0 74 90.0 75 74.5 76 55.0 77 48.5 78 45.5 79 37.0 80 32.0 81 25.5 82 24.5 83 25.0 84 19.5 85 16.0 86 13.0 87 13.0 88 10.5 89 7.0 90 5.5 91 4.5 92 5.5 93 6.0 94 3.5 95 2.0 96 2.0 97 2.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17892701844422898 2 0.18353275393954877 3 0.15291815917654095 4 0.1555887116906339 5 0.16367777655216187 6 0.16085240939957077 7 0.12748985480322092 8 0.11015061529142887 9 0.10933783843931363 10-11 0.08818628845450482 12-13 0.1342049397480779 14-15 0.1119503354639698 16-17 0.1040547889005645 18-19 0.10771228473508312 20-21 0.09927488693693431 22-23 0.09931359059655887 24-25 0.4628183617901991 26-27 0.30529446711833835 28-29 0.4002732478369493 30-31 0.270016081370574 32-33 0.2981536419176115 34-35 0.31758287904912846 36-37 0.29819234557723606 38-39 0.3452559956806716 40-41 0.34001164980154697 42-43 0.381908361345107 44-45 0.33805711499050795 46-47 0.3602149601255547 48-49 0.3562865386736643 50-51 0.37076170737324066 52-53 0.3626726425117127 54-55 0.3404954455468537 56-57 0.3545255221607479 58-59 0.3466880310867794 60-61 0.31754417538950397 62-63 0.3266201835714576 64-65 0.32811027446700225 66-67 0.3398374833332366 68-69 0.3268717573590171 70-71 0.31462204908785146 72-73 0.3009016017509536 74-75 0.3100937209117808 76-77 0.3000307694094015 78-79 0.30947446235778825 80-81 0.3182214894329333 82-83 0.34370784929569015 84-85 0.34148238886727933 86-87 0.3349221185609205 88-89 0.34761691891776825 90-91 0.33757331924520123 92-93 0.32035019071228277 94-95 0.3136544575972381 96-97 0.2986374376629182 98-99 0.3227691694388163 100-101 0.3264266652733349 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2583735.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.0392425831088 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82720640207911 43.06979680148324 2 5.64328070124695 5.53484422546051 3 1.834679979584079 2.6991394974359024 4 1.0280601868482104 2.016611715715422 5 0.6167583275655814 1.5122680620320512 6 0.4149607508844639 1.2209616555055325 7 0.3035135968693758 1.0418853832904449 8 0.28049396431034307 1.100416924713022 9 0.1750899208224573 0.7727649390962817 >10 1.5612130642750863 14.972927662519409 >50 0.16333268703793996 5.637877110173189 >100 0.13511782418224352 13.76170105211139 >500 0.013724466631862513 4.353672652849309 >1k 0.0024091075363752634 1.845114934780801 >5k 1.5902012585041066E-4 0.4600173828334701 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6018 0.23291862362045643 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5780 0.22370715262981694 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2867 0.11096339214354413 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2771 0.1072478408195887 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2631 0.10182932847215369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.87036596245358E-4 0.0 0.0 2 5.418512347435012E-4 0.0 0.0 3 5.805548943680369E-4 0.0 0.0 4 6.192585539925728E-4 0.0 0.0 5 6.579622136171086E-4 0.0 0.0 6 7.353695328661801E-4 0.0 0.0 7 8.514805117397875E-4 0.0 0.0 8 0.0011611097887360739 0.0 0.0 9 0.001509442725356896 0.0 0.0 10-11 0.001877127491789986 0.0 0.0 12-13 0.0027286080035297737 0.0 0.0 14-15 0.004025180600951723 0.0 0.0 16-17 0.0050895312406264576 0.0 0.0 18-19 0.007063417881477784 0.0 0.0 20-21 0.009056656352141377 0.0 0.0 22-23 0.011572394227736205 0.0 0.0 24-25 0.013991372954269691 0.0 0.0 26-27 0.016236185212492767 0.0 1.93518298122679E-5 28-29 0.019467940791141506 0.0 3.87036596245358E-5 30-31 0.028969689228965045 0.0 3.87036596245358E-5 32-33 0.03245301859517327 0.0 3.87036596245358E-5 34-35 0.03690393945199488 0.0 3.87036596245358E-5 36-37 0.04073560175482392 0.0 3.87036596245358E-5 38-39 0.0450317079731474 0.0 3.87036596245358E-5 40-41 0.0502179983628352 0.0 3.87036596245358E-5 42-43 0.05619771377482598 0.0 3.87036596245358E-5 44-45 0.06237094748493944 0.0 3.87036596245358E-5 46-47 0.0686602921739265 0.0 3.87036596245358E-5 48-49 0.07628491311996005 0.0 3.87036596245358E-5 50-51 0.08381277491693227 0.0 3.87036596245358E-5 52-53 0.09203730258714612 0.0 3.87036596245358E-5 54-55 0.10107460710947523 0.0 3.87036596245358E-5 56-57 0.11094404031373187 0.0 3.87036596245358E-5 58-59 0.12269060100977847 0.0 3.87036596245358E-5 60-61 0.13501771660019313 0.0 3.87036596245358E-5 62-63 0.14838983100047026 0.0 3.87036596245358E-5 64-65 0.1630004625087325 0.0 3.87036596245358E-5 66-67 0.17873350014610634 0.0 3.87036596245358E-5 68-69 0.19564699940202845 0.0 3.87036596245358E-5 70-71 0.21437957066030378 0.0 3.87036596245358E-5 72-73 0.23479575111224643 0.0 3.87036596245358E-5 74-75 0.25956609327194935 0.0 3.87036596245358E-5 76-77 0.2866199513494998 0.0 3.87036596245358E-5 78-79 0.31574445521696304 0.0 3.87036596245358E-5 80-81 0.35100348913491514 0.0 3.87036596245358E-5 82-83 0.38825576152353086 0.0 3.87036596245358E-5 84-85 0.43301654387930655 0.0 3.87036596245358E-5 86-87 0.485711576458112 0.0 3.87036596245358E-5 88-89 0.5407481804442019 0.0 3.87036596245358E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 520 0.0 72.01928 2 AGAGCGG 440 0.0 60.303368 8 ATTCGGA 105 0.0 58.69202 2 GAGCGGC 465 0.0 57.061245 9 CGGGAGA 615 0.0 54.72041 4 CGGAAAG 260 0.0 54.689705 5 GAGAGCG 635 0.0 49.993744 7 TCGGAAA 265 0.0 44.715717 4 TCGGGAA 170 0.0 44.610508 3 AAGCGTC 90 1.2243618E-8 42.116634 9 GGTCCGG 115 5.638867E-11 41.22342 1 TTGTGTT 32035 0.0 39.378754 1 GGCGCCG 405 0.0 38.125748 56-57 CGGCGAG 50 0.0016318354 37.925545 1 TCTCGGG 375 0.0 36.74898 48-49 CCGGATC 230 0.0 36.146164 60-61 GGTACGG 80 1.0927151E-5 35.5552 1 GATCGGG 1190 0.0 35.057228 1 CGACCGA 55 0.0026051525 34.477768 1 CGAGCGG 55 0.0026051525 34.477768 1 >>END_MODULE