FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583735
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60180.23291862362045643No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC57800.22370715262981694No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28670.11096339214354413No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA27710.1072478408195887No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA26310.10182932847215369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5200.072.019282
AGAGCGG4400.060.3033688
ATTCGGA1050.058.692022
GAGCGGC4650.057.0612459
CGGGAGA6150.054.720414
CGGAAAG2600.054.6897055
GAGAGCG6350.049.9937447
TCGGAAA2650.044.7157174
TCGGGAA1700.044.6105083
AAGCGTC901.2243618E-842.1166349
GGTCCGG1155.638867E-1141.223421
TTGTGTT320350.039.3787541
GGCGCCG4050.038.12574856-57
CGGCGAG500.001631835437.9255451
TCTCGGG3750.036.7489848-49
CCGGATC2300.036.14616460-61
GGTACGG801.0927151E-535.55521
GATCGGG11900.035.0572281
CGACCGA550.002605152534.4777681
CGAGCGG550.002605152534.4777681
Produced by FastQC (version 0.11.2)