##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34344-1.34200000035854.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4672105 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74118004625324 33.0 33.0 33.0 27.0 33.0 2 31.6243905049223 33.0 33.0 33.0 27.0 33.0 3 31.55082987218823 33.0 33.0 33.0 27.0 33.0 4 35.185313258156654 37.0 37.0 37.0 33.0 37.0 5 35.22531449956711 37.0 37.0 37.0 33.0 37.0 6 35.32155976802748 37.0 37.0 37.0 33.0 37.0 7 35.10165610575961 37.0 37.0 37.0 33.0 37.0 8 35.19866719604975 37.0 37.0 37.0 33.0 37.0 9 34.98547399940712 37.0 37.0 37.0 33.0 37.0 10-11 34.92622907661536 37.0 37.0 37.0 33.0 37.0 12-13 34.89139028339474 37.0 37.0 37.0 33.0 37.0 14-15 36.51749115227504 40.0 37.0 40.0 33.0 40.0 16-17 36.48913006021911 40.0 37.0 40.0 33.0 40.0 18-19 36.526997894953126 40.0 37.0 40.0 33.0 40.0 20-21 36.540169794985346 40.0 37.0 40.0 33.0 40.0 22-23 36.671985218654115 40.0 37.0 40.0 33.0 40.0 24-25 36.3362934908355 40.0 37.0 40.0 33.0 40.0 26-27 36.26108831030125 40.0 37.0 40.0 30.0 40.0 28-29 36.11619880118276 40.0 37.0 40.0 30.0 40.0 30-31 36.1237459346483 40.0 37.0 40.0 27.0 40.0 32-33 36.02397334820172 40.0 37.0 40.0 27.0 40.0 34-35 36.01257741424904 40.0 37.0 40.0 27.0 40.0 36-37 35.90949133206553 40.0 37.0 40.0 27.0 40.0 38-39 35.797661653580136 38.5 37.0 40.0 27.0 40.0 40-41 35.677080994541 37.0 37.0 40.0 27.0 40.0 42-43 35.583157484688385 37.0 37.0 40.0 27.0 40.0 44-45 35.43974932070234 37.0 37.0 40.0 27.0 40.0 46-47 35.37376375316907 37.0 37.0 40.0 27.0 40.0 48-49 35.26391968930493 37.0 37.0 40.0 27.0 40.0 50-51 34.11712846778914 37.0 35.0 38.5 24.5 38.5 52-53 34.57246894065951 37.0 35.0 38.5 27.0 40.0 54-55 35.318799128016174 37.0 37.0 40.0 27.0 40.0 56-57 35.38778238074701 37.0 37.0 40.0 27.0 40.0 58-59 35.10155197710668 37.0 37.0 40.0 27.0 40.0 60-61 34.91056194156595 37.0 35.0 40.0 27.0 40.0 62-63 34.61414212651471 37.0 33.0 40.0 27.0 40.0 64-65 34.42538063677935 37.0 33.0 40.0 27.0 40.0 66-67 34.19695501278332 37.0 33.0 37.0 27.0 40.0 68-69 34.04480582093082 37.0 33.0 37.0 27.0 40.0 70-71 33.67688033552328 37.0 33.0 37.0 22.0 40.0 72-73 33.54167414901848 37.0 33.0 37.0 27.0 38.5 74-75 33.36171458047283 37.0 33.0 37.0 27.0 37.0 76-77 33.09626239136321 37.0 33.0 37.0 22.0 37.0 78-79 32.81378500697223 37.0 33.0 37.0 22.0 37.0 80-81 32.5131298419021 37.0 33.0 37.0 22.0 37.0 82-83 32.282994496056915 37.0 33.0 37.0 22.0 37.0 84-85 32.12039937030525 37.0 33.0 37.0 22.0 37.0 86-87 31.963215402907252 33.0 33.0 37.0 22.0 37.0 88-89 31.81572010903008 33.0 33.0 37.0 22.0 37.0 90-91 31.67332262438451 33.0 33.0 37.0 15.0 37.0 92-93 31.54168602803233 33.0 33.0 37.0 15.0 37.0 94-95 31.39665771638266 33.0 33.0 37.0 15.0 37.0 96-97 31.232526238173158 33.0 33.0 37.0 15.0 37.0 98-99 31.089871267876042 33.0 33.0 37.0 15.0 37.0 100-101 29.877983799593544 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.06866691093746624 2306 2 0.006734902834157452 2306 3 0.07774604226753823 2306 4 0.08595161386875816 2306 5 0.08473148846679379 2306 6 0.025408515277050014 2306 7 0.2534854954880785 2306 8 0.04387288556828395 2306 9 0.14617572423521352 2306 10-11 0.1805751944580365 2306 12-13 0.21227288602283778 2306 14-15 0.4742847290734744 2306 16-17 0.4641814939969251 2306 18-19 0.4091340127753469 2306 20-21 0.3829782289329202 2306 22-23 0.2247390690388542 2306 24-25 0.25527303292543735 2306 26-27 0.35044874473910426 2306 28-29 0.2435796949884974 2306 30-31 0.0871520899605045 2306 32-33 0.16370549675064439 2306 34-35 0.22843931109756 2306 36-37 0.12212950505892195 2306 38-39 0.20437234259935622 2306 40-41 0.2975764615570924 2306 42-43 0.2959373690389242 2306 44-45 0.2002683381279553 2306 46-47 0.16991107062802513 2306 48-49 0.1720432009525794 2306 50-51 0.23528012025374068 2306 52-53 0.30093304405885135 2306 54-55 0.346616776668192 2306 56-57 0.2959074036018663 2306 58-59 0.48956132612489967 2306 60-61 0.31625910725760775 2306 62-63 0.47206924543346673 2306 64-65 0.36498556923422143 2306 66-67 0.5006671150063369 2306 68-69 0.3743202412301869 2306 70-71 0.30632645005434966 2306 72-73 0.27972956017178063 2306 74-75 0.28765700946414086 2306 76-77 0.33872346960687594 2306 78-79 0.3363087201020747 2306 80-81 0.4328538391783425 2306 82-83 0.4357920867103573 2306 84-85 0.34975053056334815 2306 86-87 0.23670328319354894 2306 88-89 0.3651644158681293 2306 90-91 0.4415208728905142 2306 92-93 0.39713312078140817 2306 94-95 0.454234711414653 2306 96-97 0.3646263429028238 2306 98-99 0.2909495339573809 2306 100-101 0.12503460391005206 2307 1 0.18978973793869258 2307 2 0.020245791538684443 2307 3 0.07934244073702601 2307 4 0.15644115051922114 2307 5 0.12256808413238929 2307 6 0.09690755152227837 2307 7 0.1839465410183081 2307 8 0.08355256643707776 2307 9 0.1260444102523408 2307 10-11 0.22876910028981 2307 12-13 0.25477018444384925 2307 14-15 0.3757870872070441 2307 16-17 0.4004318939647433 2307 18-19 0.4319688816338001 2307 20-21 0.36798727970152356 2307 22-23 0.21482615424588403 2307 24-25 0.08646919294704247 2307 26-27 0.17099887531542635 2307 28-29 0.12594676676427952 2307 30-31 0.1756216765997678 2307 32-33 0.16160057633225478 2307 34-35 0.10939529883123811 2307 36-37 0.09830010546090762 2307 38-39 0.1290916346515374 2307 40-41 0.15648344533037317 2307 42-43 -0.03123995273828939 2307 44-45 -0.014992062472138912 2307 46-47 0.025308636237113546 2307 48-49 0.007426568901891528 2307 50-51 -0.043367214954734834 2307 52-53 -0.008043467053070685 2307 54-55 0.06672894249149408 2307 56-57 -0.0013646003757870062 2307 58-59 0.05648863449002306 2307 60-61 0.0776390297356997 2307 62-63 0.13092310773340898 2307 64-65 0.09084445819549813 2307 66-67 0.15481219458795437 2307 68-69 0.06729608796443642 2307 70-71 0.08347354671914076 2307 72-73 0.02382602330672512 2307 74-75 0.028458109064260384 2307 76-77 0.1146602445155267 2307 78-79 0.2755615131707003 2307 80-81 0.21544654548988973 2307 82-83 0.10811043426370759 2307 84-85 -0.04998867605380042 2307 86-87 0.13481615363080124 2307 88-89 0.25024013193026917 2307 90-91 0.36487428684330325 2307 92-93 0.315417242107209 2307 94-95 0.21990650405601642 2307 96-97 0.17989043182884146 2307 98-99 0.14456466816454494 2307 100-101 0.19201184837612573 2201 1 0.02425537734130856 2201 2 -0.048497125208434966 2201 3 -0.006770291068985301 2201 4 -0.004045106920820274 2201 5 -0.01660860052749058 2201 6 0.08443441541468388 2201 7 0.1540519608086015 2201 8 0.02969465963799678 2201 9 0.08244452274489333 2201 10-11 0.05913028322452618 2201 12-13 0.09646472939469675 2201 14-15 0.3031431869896153 2201 16-17 0.24832265866222514 2201 18-19 0.3207475924188188 2201 20-21 0.19991622260735653 2201 22-23 0.21833026568067027 2201 24-25 0.09275896410026263 2201 26-27 0.16970783442354076 2201 28-29 0.12480568095932654 2201 30-31 0.11145396406019614 2201 32-33 0.08041647681832842 2201 34-35 0.03495571530552155 2201 36-37 0.008733002395899803 2201 38-39 -0.00326765521825223 2201 40-41 0.049564996132509975 2201 42-43 -0.03218548829516976 2201 44-45 -0.04282903642256031 2201 46-47 -0.12717538184564603 2201 48-49 -0.06262097808893685 2201 50-51 -0.10191124445464794 2201 52-53 0.0016252780673511324 2201 54-55 0.2220383914458992 2201 56-57 0.27720420476865826 2201 58-59 0.17686909436628184 2201 60-61 0.2301186929296506 2201 62-63 0.21716255106318982 2201 64-65 0.11665703847236131 2201 66-67 0.2030923807883056 2201 68-69 0.1266944626989499 2201 70-71 0.09207088425555554 2201 72-73 0.11894576700840531 2201 74-75 0.16860063460595143 2201 76-77 0.19866085524933652 2201 78-79 0.26313108645455685 2201 80-81 0.11714642124437802 2201 82-83 0.17307215321702074 2201 84-85 0.09530101324850193 2201 86-87 0.15340323881452633 2201 88-89 0.16690256613471632 2201 90-91 0.2118854164699826 2201 92-93 0.11246447008931071 2201 94-95 0.1393041232435479 2201 96-97 0.14286222893929335 2201 98-99 0.02766937858017826 2201 100-101 -0.011551709914325414 1101 1 0.10581465003358304 1101 2 -0.06136756073896876 1101 3 -0.010085640197139867 1101 4 -0.00835048955132578 1101 5 -0.018429877225614177 1101 6 0.019858487784567558 1101 7 0.14952042228691198 1101 8 0.16396269045775114 1101 9 0.026661006862852332 1101 10-11 0.07955889675658767 1101 12-13 0.05993694409843897 1101 14-15 0.23968059594082547 1101 16-17 0.28391093464458805 1101 18-19 0.1525790341024802 1101 20-21 0.13731374571916888 1101 22-23 0.22798399454622142 1101 24-25 0.05029398518208694 1101 26-27 0.09417597711829018 1101 28-29 0.18230040298607264 1101 30-31 0.12750441607123975 1101 32-33 0.2513554448016322 1101 34-35 0.19492552108813754 1101 36-37 0.0012109131004152118 1101 38-39 0.07347377521775655 1101 40-41 0.07803537632702984 1101 42-43 0.15807296698791617 1101 44-45 0.0623533017647091 1101 46-47 0.03527612395561874 1101 48-49 -0.15217690656112381 1101 50-51 0.0476277223256929 1101 52-53 0.030252042654709044 1101 54-55 0.17066190804971626 1101 56-57 0.20040924275201633 1101 58-59 -0.016793728853194523 1101 60-61 0.16358134412629255 1101 62-63 0.2000577073573524 1101 64-65 0.15328166179649116 1101 66-67 -0.012773417343495908 1101 68-69 0.1832376458431142 1101 70-71 0.2175416914997541 1101 72-73 0.10537460910227026 1101 74-75 0.2714949488303091 1101 76-77 0.24480447626979895 1101 78-79 0.06147100597674893 1101 80-81 0.17264363339183575 1101 82-83 0.17674478053496756 1101 84-85 0.1711528570806422 1101 86-87 0.16133012309609285 1101 88-89 0.15199612610511792 1101 90-91 0.03590365329610279 1101 92-93 0.1837697384389152 1101 94-95 0.29954218840772384 1101 96-97 0.2200686022047833 1101 98-99 0.2262691367858487 1101 100-101 0.17123939061076854 2304 1 0.19989026334236115 2304 2 0.2740969387987313 2304 3 0.2774568793882004 2304 4 0.39606143944669014 2304 5 0.3512450002296674 2304 6 0.3140339030853454 2304 7 0.3568425688596548 2304 8 0.25883735809477315 2304 9 0.3586047730155073 2304 10-11 0.4247588784560108 2304 12-13 0.40835655082752 2304 14-15 0.4215388707282628 2304 16-17 0.48520187021910743 2304 18-19 0.47162461637474706 2304 20-21 0.3951896443892906 2304 22-23 0.4334122440426924 2304 24-25 0.31292909824667703 2304 26-27 0.4304522988296853 2304 28-29 0.36825449619772144 2304 30-31 0.3899650352479114 2304 32-33 0.4174039349507055 2304 34-35 0.2910110060694322 2304 36-37 0.2933636888832183 2304 38-39 0.29394627061867595 2304 40-41 0.36925397141715877 2304 42-43 0.37786557461819825 2304 44-45 0.3926798074926765 2304 46-47 0.29184597843409676 2304 48-49 0.22501397592043304 2304 50-51 0.15073286234107997 2304 52-53 0.29731015018337814 2304 54-55 0.409863768750391 2304 56-57 0.4612834571305271 2304 58-59 0.523246234503091 2304 60-61 0.5359997123743625 2304 62-63 0.589816405718075 2304 64-65 0.49961585376743045 2304 66-67 0.4865232871691916 2304 68-69 0.48345703998757017 2304 70-71 0.3761433357854571 2304 72-73 0.42064144111252944 2304 74-75 0.24850644308318692 2304 76-77 0.3528209808380254 2304 78-79 0.3474228648154494 2304 80-81 0.3744649665464195 2304 82-83 0.29107338870149135 2304 84-85 0.3172478647466619 2304 86-87 0.4028594556250482 2304 88-89 0.484549024884835 2304 90-91 0.2183524409359734 2304 92-93 0.4073485442410494 2304 94-95 0.42696741405279326 2304 96-97 0.1894944002749206 2304 98-99 0.17615534336320593 2304 100-101 0.19364695938955734 2202 1 0.131427823444195 2202 2 0.04768055649654812 2202 3 0.02305866830372949 2202 4 0.017069492765209304 2202 5 -0.04181757255770435 2202 6 0.07022102969793309 2202 7 0.12375286573762878 2202 8 0.04450393413313947 2202 9 0.032665207071786995 2202 10-11 0.10938774827256026 2202 12-13 0.05879592848729942 2202 14-15 0.1352802907235855 2202 16-17 0.14255959225966564 2202 18-19 0.08664015161728145 2202 20-21 0.01354769211017981 2202 22-23 0.1794464681020358 2202 24-25 0.06028610786150779 2202 26-27 0.07909411007758393 2202 28-29 0.11801706942127765 2202 30-31 0.022101469135527907 2202 32-33 -0.02371611459923173 2202 34-35 -0.08549806427316042 2202 36-37 -0.13226044693711003 2202 38-39 -0.14317699793237892 2202 40-41 -0.036560658317746686 2202 42-43 -0.19656161329208288 2202 44-45 -0.09066226153701251 2202 46-47 -0.057574339656717655 2202 48-49 -0.03938254860518242 2202 50-51 -0.07021139979668334 2202 52-53 -0.017430628628787304 2202 54-55 0.05307700503873747 2202 56-57 0.08148145332413748 2202 58-59 -0.006908347339361853 2202 60-61 0.12767472702270766 2202 62-63 0.15121369064910795 2202 64-65 0.0833748008894517 2202 66-67 0.02201513716431691 2202 68-69 0.04573002698507622 2202 70-71 0.013440894200734022 2202 72-73 0.078025879117682 2202 74-75 0.1361725727650196 2202 76-77 0.20585158897335276 2202 78-79 0.13981662978184062 2202 80-81 0.0606746581533244 2202 82-83 0.07169010220894023 2202 84-85 -0.022095445939285696 2202 86-87 -1.755869969102264E-4 2202 88-89 -0.0031591597235696156 2202 90-91 0.09376546611368042 2202 92-93 -0.00768992674795399 2202 94-95 0.0794838761757326 2202 96-97 0.03725142239355961 2202 98-99 0.11982994800327518 2202 100-101 0.12959934172663168 1102 1 -0.0025715889932449443 1102 2 -0.09376096529048183 1102 3 -0.11139531820331428 1102 4 -0.07010485593218618 1102 5 0.0025680573408379814 1102 6 0.0017406133678932179 1102 7 -0.032391884495822865 1102 8 0.1467859851461597 1102 9 -0.003992401338756224 1102 10-11 -0.0035447922010547472 1102 12-13 0.08445562484336477 1102 14-15 0.006670846704651012 1102 16-17 -0.04875458058864268 1102 18-19 -0.006911218137936714 1102 20-21 -0.00943702077107389 1102 22-23 0.026213300195543354 1102 24-25 -0.043429307261924066 1102 26-27 0.06739728711227855 1102 28-29 -0.0637809507027427 1102 30-31 -0.16415848532837174 1102 32-33 -0.027135518852766438 1102 34-35 -0.010649938702648853 1102 36-37 -0.014570354710777167 1102 38-39 0.02725929349865197 1102 40-41 0.0485379420242964 1102 42-43 0.009579989284794976 1102 44-45 -0.10276185339087363 1102 46-47 -0.2048954216112051 1102 48-49 -0.13484493935526842 1102 50-51 -0.18496223654872068 1102 52-53 -0.09886247137186643 1102 54-55 0.09567620868163118 1102 56-57 0.20643200888552116 1102 58-59 0.19421261185652128 1102 60-61 0.2559843567533733 1102 62-63 0.24454105217845523 1102 64-65 0.24211859738296226 1102 66-67 0.2654874220482242 1102 68-69 0.2426394216907184 1102 70-71 0.1927867587046066 1102 72-73 0.1819186122278822 1102 74-75 0.18370376079553807 1102 76-77 0.20013098546266406 1102 78-79 0.21711507483949077 1102 80-81 0.17806362456782665 1102 82-83 0.22776953856856608 1102 84-85 0.15467734047123827 1102 86-87 0.07573408575173346 1102 88-89 0.30699334423722746 1102 90-91 0.23313989560092807 1102 92-93 0.17086443328100032 1102 94-95 0.2301944596463379 1102 96-97 0.09838048680510525 1102 98-99 0.05536554799908089 1102 100-101 0.1330007781072453 2305 1 0.08244075734392808 2305 2 0.08030494161401691 2305 3 0.04700315952602452 2305 4 0.035509637867988886 2305 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-1.3380869418086547 1213 90-91 -1.295281785691337 1213 92-93 -1.30418508085641 1213 94-95 -1.460957711379809 1213 96-97 -1.387745994362696 1213 98-99 -1.415329812791775 1213 100-101 -1.2924351970976424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1909.0 3 390.0 4 416.0 5 563.0 6 1049.0 7 4238.0 8 13617.0 9 29402.0 10 42740.0 11 38433.0 12 27087.0 13 19856.0 14 15732.0 15 13563.0 16 13213.0 17 13337.0 18 14191.0 19 15748.0 20 17178.0 21 19228.0 22 21017.0 23 23089.0 24 26009.0 25 29882.0 26 34413.0 27 40866.0 28 49571.0 29 60442.0 30 74480.0 31 96213.0 32 129735.0 33 184632.0 34 278539.0 35 466757.0 36 904672.0 37 1515289.0 38 434249.0 39 360.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.898640331652366 24.112025314826997 26.345600742495062 14.643733611025572 2 29.483567269646898 23.085257186973912 34.833313674819586 12.597861868559605 3 38.508899173006576 19.358493737412545 26.80484335920466 15.327763730376217 4 28.252470895954378 18.456510087258536 36.57492013807002 16.716098878717066 5 33.075095959927324 17.43348843871022 33.726156890139336 15.76525871122312 6 25.328484070910495 18.387861092344746 40.834467355491775 15.449187481252991 7 32.710338063524944 18.901104930693204 31.836115664501612 16.55244134128024 8 27.755370122745664 27.990511211684836 27.99576133168758 16.258357333881918 9 31.88931030662444 25.33155842154855 25.173924997262414 17.605206274564594 10-11 25.361227829151566 28.710924005173975 28.813139550142335 17.114708615532116 12-13 26.425314760470386 25.1697667046129 28.795457512186058 19.60946102273066 14-15 24.33856747649468 25.694428744288878 30.225920446695646 19.741083332520795 16-17 23.6734661517126 26.893000464555765 31.424026275685563 18.009507108046073 18-19 22.922222453173895 27.31290182465868 31.437471299195295 18.32740442297213 20-21 22.63976537704391 28.227680687798802 30.630766140372025 18.501787794785262 22-23 23.8457737687875 27.240240442282154 30.265192307115434 18.648793481814913 24-25 22.407208967102804 28.502255732230747 30.223865752374167 18.86666954829228 26-27 22.23104953748566 28.75066482209178 29.898592756557985 19.119692883864573 28-29 22.006988811905202 28.61639210671063 29.65925539421586 19.71736368716831 30-31 22.48646547810317 28.12943266121326 29.794758564388356 19.589343296295212 32-33 22.40819992512425 27.744320777333943 30.574003774699555 19.273475522842258 34-35 21.45348151263815 28.020046525304377 30.186213939748992 20.340258022308486 36-37 20.608302431314225 28.45304504877365 30.425887049573713 20.512765470338408 38-39 21.04891266062675 28.78386713790147 30.46257669449518 19.7046435069766 40-41 21.612146609856723 28.008151029203272 30.64626124634893 19.73344111459107 42-43 22.022111802149688 28.137437969279045 30.82482891799859 19.015621310572676 44-45 21.091228567124283 28.97200044969538 30.921593119961393 19.015177863218945 46-47 21.838937997400222 28.925589211739027 30.031575204115395 19.203897586745352 48-49 21.080828959230143 28.805716788372592 30.230467643306874 19.882986609090388 50-51 20.809386764834308 28.417700981193022 30.31477942196112 20.458132832011554 52-53 22.459711264735212 28.01281978990624 29.458526057114444 20.068942888244106 54-55 22.921232490590956 28.254648147452176 29.203251421423086 19.620867940533778 56-57 23.131534398114482 28.26486723742957 28.77097818941669 19.832620175039256 58-59 22.368075579384353 28.13289793172783 29.17143148926958 20.327594999618242 60-61 21.022106078602633 28.48528551663012 29.026568604464416 21.466039800302827 62-63 22.104795274673304 28.454750959972902 29.177099365314714 20.26335440003908 64-65 21.160038686065246 28.921688203541613 29.770780339336227 20.147492771056914 66-67 21.175232200270035 28.422464147986236 29.18546234854018 21.216841303203545 68-69 21.29297994269341 27.90146822370184 30.741889296585654 20.063662537019095 70-71 21.094621309184948 29.845644504108076 29.3680265072567 19.691707679450275 72-73 20.723983658636776 31.35381266810934 28.322077831747844 19.600125841506035 74-75 21.18779524254142 30.90684210198627 28.145680932916516 19.759681722555793 76-77 21.283338199179102 30.321725428909996 28.514679111782787 19.880257260128115 78-79 21.142526150454692 30.08573261420388 29.013425495947274 19.75831573939415 80-81 20.601606723711026 30.382144393858585 28.92221844331249 20.094030439117898 82-83 19.9366590771958 30.268748450329415 29.16687024689387 20.627722225580925 84-85 20.02075034109635 29.889317912535446 29.312197708528366 20.777734037839842 86-87 20.028801904550633 29.88816069280907 29.838210476596146 20.24482692604415 88-89 20.195194147118006 30.18021142900466 29.55638611512681 20.06820830875052 90-91 20.395462341986974 29.76683770360522 29.271905781575384 20.565794172832423 92-93 20.036488948909355 30.538828233222226 29.05748727186489 20.36719554600353 94-95 19.690930605811783 30.981751671628206 29.082074376626288 20.245243345933726 96-97 20.1048481368912 31.067212223881963 28.846128457536473 19.981811181690368 98-99 20.545882855135524 30.36522096500736 28.436447330675374 20.65244884918174 100-101 20.532670020664458 30.275296731532407 27.946194027759542 21.245839220043592 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 952.0 1 817.0 2 554.5 3 478.5 4 540.5 5 553.5 6 635.0 7 848.0 8 1164.5 9 1483.5 10 1742.5 11 1968.0 12 1994.0 13 1881.0 14 1774.0 15 1644.0 16 1511.0 17 1394.0 18 1434.0 19 1594.0 20 1812.5 21 2203.0 22 2753.0 23 3627.5 24 4951.5 25 6665.0 26 9315.0 27 13219.5 28 17410.5 29 21721.5 30 27149.0 31 33916.5 32 46498.0 33 67739.5 34 98203.5 35 136180.5 36 186373.5 37 247043.0 38 302155.0 39 326168.5 40 321248.0 41 298703.0 42 276199.5 43 277445.0 44 273329.5 45 255131.5 46 241398.5 47 214640.5 48 173418.5 49 143835.5 50 122555.0 51 104207.0 52 88687.5 53 72504.5 54 56304.0 55 42438.5 56 32227.5 57 23997.5 58 18486.0 59 14509.5 60 10849.0 61 8027.5 62 5903.5 63 4304.5 64 3150.5 65 2289.0 66 1629.5 67 1188.5 68 912.0 69 638.0 70 469.5 71 373.0 72 275.0 73 193.5 74 162.5 75 144.0 76 118.5 77 101.0 78 82.5 79 69.5 80 64.5 81 51.0 82 41.5 83 35.5 84 27.5 85 24.5 86 16.5 87 15.0 88 14.0 89 13.0 90 17.5 91 15.0 92 8.0 93 5.5 94 5.5 95 3.0 96 1.5 97 1.5 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19068492681564306 2 0.1959502194407018 3 0.16564268140377839 4 0.16705532088855024 5 0.17506027796892407 6 0.17182833005679451 7 0.13854568765042738 8 0.11868312034939282 9 0.11947505460600735 10-11 0.09669089200692194 12-13 0.14408922744672906 14-15 0.12117664307630073 16-17 0.11303256241030542 18-19 0.11609328129397777 20-21 0.10792779699942531 22-23 0.10785288429947529 24-25 0.4698524540865413 26-27 0.3177154623023241 28-29 0.4026129549742568 30-31 0.2664002628365587 32-33 0.2936791874326455 34-35 0.31333842026238706 36-37 0.2931976057901096 38-39 0.34179454442911705 40-41 0.33733188787495144 42-43 0.38008563591785716 44-45 0.3343139762483934 46-47 0.3576867386328004 48-49 0.352817413136049 50-51 0.36836714928281794 52-53 0.359634468831501 54-55 0.3356088957761009 56-57 0.3511586319228699 58-59 0.34289683129981025 60-61 0.31362736924790857 62-63 0.3226061914276327 64-65 0.32385830369822594 66-67 0.33543766674764375 68-69 0.32210320615653976 70-71 0.3106201594356291 72-73 0.2967506081305964 74-75 0.3056866230532062 76-77 0.295049019660303 78-79 0.3050231105679346 80-81 0.31414105633328016 82-83 0.3397504979019093 84-85 0.3380917166887302 86-87 0.3310820283362638 88-89 0.34404192542761775 90-91 0.33402502726287187 92-93 0.3168700189743167 94-95 0.3091861163223001 96-97 0.2942677872179671 98-99 0.318849854615853 100-101 0.3226704023133042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4672105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.213441394185956 #Duplication Level Percentage of deduplicated Percentage of total 1 91.78360260687288 46.08770550447465 2 3.6713866603490826 3.6870591780966944 3 1.2545067317124468 1.889793007543641 4 0.581698295663842 1.1683629311365673 5 0.49686627339218326 1.2474682749862984 6 0.3700523176118083 1.1148960217909933 7 0.24764734863000462 0.8704657938800789 8 0.20855999120205057 0.8378011916316884 9 0.11628870592997725 0.5255330508018574 >10 0.9768664175216132 9.859310880976759 >50 0.13081985095042642 4.627664784697975 >100 0.1304882919916489 14.352301043873727 >500 0.02492502617429701 8.565294970993765 >1k 0.006119207099802925 4.551745917026905 >5k 1.7227489772708197E-4 0.6145974480884389 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8115 0.17369044574126652 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7078 0.1514948829275027 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6645 0.14222711176225705 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 6581 0.1408572795345995 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014126394847718533 0.0 0.0 2 0.0015624648846718984 0.0 0.0 3 0.001690886656014794 0.0 0.0 4 0.00181930842735769 0.0 0.0 5 0.002054748341486332 0.0 0.0 6 0.0022259773699435264 0.0 0.0 7 0.0026112426839722137 0.0 0.0 8 0.0029537007408866026 0.0 0.0 9 0.003360369683472439 0.0 0.0 10-11 0.005147572667994406 0.0 0.0 12-13 0.00727723370943076 0.0 2.1403628557149293E-5 14-15 0.009867072764845823 0.0 2.1403628557149293E-5 16-17 0.012103751949067926 0.0 2.1403628557149293E-5 18-19 0.01471499463304014 0.0 4.280725711429859E-5 20-21 0.017657993559648165 0.0 4.280725711429859E-5 22-23 0.02148924307137789 0.0 4.280725711429859E-5 24-25 0.025887688739872072 0.0 4.280725711429859E-5 26-27 0.030639294279559214 0.0 4.280725711429859E-5 28-29 0.03595809597601081 0.0 4.280725711429859E-5 30-31 0.056056103191174 0.0 5.3509071392873234E-5 32-33 0.06426439474284076 0.0 7.491269995002253E-5 34-35 0.07110285406684995 0.0 8.561451422859717E-5 36-37 0.07845500047623075 0.0 8.561451422859717E-5 38-39 0.08689873194202613 0.0 8.561451422859717E-5 40-41 0.09596316863597887 0.0 8.561451422859717E-5 42-43 0.10526304524406022 0.0 8.561451422859717E-5 44-45 0.11584713956557055 0.0 8.561451422859717E-5 46-47 0.12723386995797398 0.0 8.561451422859717E-5 48-49 0.13941253460699193 0.0 8.561451422859717E-5 50-51 0.15234032625551008 0.0 8.561451422859717E-5 52-53 0.1668947936743716 0.0 8.561451422859717E-5 54-55 0.1822197917212905 0.0 8.561451422859717E-5 56-57 0.19865777845318117 0.0 8.561451422859717E-5 58-59 0.21641208834133652 0.0 8.561451422859717E-5 60-61 0.23474429620053489 0.0 8.561451422859717E-5 62-63 0.25585897577216266 0.0 1.0701814278574647E-4 64-65 0.2785361202284623 0.0 1.0701814278574647E-4 66-67 0.30407064909714143 0.0 1.0701814278574647E-4 68-69 0.33154220635024256 0.0 1.0701814278574647E-4 70-71 0.3621172897441303 0.0 1.2842177134289576E-4 72-73 0.39406220536567566 0.0 1.2842177134289576E-4 74-75 0.42944240337064343 0.0 1.3912358562147043E-4 76-77 0.4675622658309263 0.0 1.4982539990004507E-4 78-79 0.5112256680875109 0.0 1.4982539990004507E-4 80-81 0.5592554105697539 0.0 1.4982539990004507E-4 82-83 0.6139844887903846 0.0 1.4982539990004507E-4 84-85 0.6701262064957871 0.0 1.605272141786197E-4 86-87 0.7318007621832129 0.0 1.7122902845719435E-4 88-89 0.7997358792236048 0.0 1.7122902845719435E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1730 0.0 77.56549 9 CGGGAGA 2155 0.0 71.30332 4 AGAGCGG 2000 0.0 65.90874 8 AAGCGGC 685 0.0 61.606663 9 GAGAGCG 2400 0.0 60.258934 7 TCGGGAG 2645 0.0 59.34913 3 ATTCGGA 540 0.0 55.339848 2 GATCCGG 525 0.0 53.311523 1 CGGAAAG 940 0.0 50.9572 5 TTGTGTT 64050 0.0 50.4082 1 GATCGGG 3960 0.0 50.19347 1 TCGGGAA 955 0.0 47.67381 3 ATCGGGA 4065 0.0 46.558983 2 TCGGAGA 610 0.0 44.31564 4 GGTCCGG 280 0.0 44.049774 1 GAGCGTC 7165 0.0 43.809673 9 AGAGCGT 7175 0.0 43.74861 8 CCGGATC 1340 0.0 43.066113 60-61 GGCGCCG 2225 0.0 42.91504 56-57 GTTCGGA 370 0.0 42.306137 2 >>END_MODULE