FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4672105
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81150.17369044574126652No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG70780.1514948829275027No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA66450.14222711176225705No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT65810.1408572795345995Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC17300.077.565499
CGGGAGA21550.071.303324
AGAGCGG20000.065.908748
AAGCGGC6850.061.6066639
GAGAGCG24000.060.2589347
TCGGGAG26450.059.349133
ATTCGGA5400.055.3398482
GATCCGG5250.053.3115231
CGGAAAG9400.050.95725
TTGTGTT640500.050.40821
GATCGGG39600.050.193471
TCGGGAA9550.047.673813
ATCGGGA40650.046.5589832
TCGGAGA6100.044.315644
GGTCCGG2800.044.0497741
GAGCGTC71650.043.8096739
AGAGCGT71750.043.748618
CCGGATC13400.043.06611360-61
GGCGCCG22250.042.9150456-57
GTTCGGA3700.042.3061372
Produced by FastQC (version 0.11.2)