FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34311-1.34200000035761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34311-1.34200000035761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534681
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG55180.21769997881390205No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG54930.21671366140354545No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC53500.21107192581630588No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT50900.20081422474859753No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC49730.19619825926812884No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT46410.18309996405859358No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA38880.15339208365865367No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG38300.15110382726662644No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT37720.1488155708745992No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA36780.14510701741165852No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG32040.12640643931129794No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA28200.11125660388822103No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA27080.10683790188982362No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA26800.10573322639022426No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT26430.10427347662289653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC3700.055.065059
CGGGAGA5100.052.0401734
GGTACGG558.308707E-751.714511
AGAGCGG4150.051.377598
TTGTGTT348600.049.0368081
ACGTCCC459.728297E-442.132752
AAGCGTC1155.638867E-1141.201359
ATCGGGA7950.041.1390532
ACCGGAA608.403753E-539.4947743
TCGGGAA1950.038.887163
TGTGTTT596350.037.9573631
GAACCGG1003.0871888E-837.9239771
ATTCGGA757.0271235E-637.919482
ACTGGCG3150.037.618532
CTCCGGG1650.037.4366948-49
TCGGAGA1401.0913936E-1137.237934
AAGCGGC1800.036.852329
GAGAGCG6750.036.5013477
ACGGCGA651.3443815E-436.4653631
AACGCGT651.3482946E-436.447358
Produced by FastQC (version 0.11.2)