##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34311-1.34200000035761.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2534681 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95737727942885 33.0 33.0 33.0 27.0 33.0 2 31.92419874532535 33.0 33.0 33.0 33.0 33.0 3 31.892997580366128 33.0 33.0 33.0 33.0 33.0 4 35.624044603640456 37.0 37.0 37.0 33.0 37.0 5 35.65168082295168 37.0 37.0 37.0 33.0 37.0 6 35.62595214151209 37.0 37.0 37.0 33.0 37.0 7 35.54959302571014 37.0 37.0 37.0 33.0 37.0 8 35.498665118016824 37.0 37.0 37.0 33.0 37.0 9 35.44692409025041 37.0 37.0 37.0 33.0 37.0 10-11 35.35709878284486 37.0 37.0 37.0 33.0 37.0 12-13 35.33180230569448 37.0 37.0 37.0 33.0 37.0 14-15 36.952699767741976 40.0 37.0 40.0 33.0 40.0 16-17 36.93251004761546 40.0 37.0 40.0 33.0 40.0 18-19 36.96128723890699 40.0 37.0 40.0 33.0 40.0 20-21 36.96835361136175 40.0 37.0 40.0 33.0 40.0 22-23 36.92553540267986 40.0 37.0 40.0 33.0 40.0 24-25 36.70377021802744 40.0 37.0 40.0 33.0 40.0 26-27 36.60041105764394 40.0 37.0 40.0 33.0 40.0 28-29 36.546315690219004 40.0 37.0 40.0 33.0 40.0 30-31 36.58618204815517 40.0 37.0 40.0 33.0 40.0 32-33 36.48653834545649 40.0 37.0 40.0 33.0 40.0 34-35 36.46179677048117 40.0 37.0 40.0 33.0 40.0 36-37 36.37401314011507 38.5 37.0 40.0 33.0 40.0 38-39 36.31667594462577 37.0 37.0 40.0 33.0 40.0 40-41 36.14119054823861 37.0 37.0 40.0 33.0 40.0 42-43 36.06835712265173 37.0 37.0 40.0 33.0 40.0 44-45 35.960913424608464 37.0 37.0 40.0 27.0 40.0 46-47 35.91402428944707 37.0 37.0 40.0 27.0 40.0 48-49 35.80172317542129 37.0 37.0 40.0 27.0 40.0 50-51 34.595675155966376 37.0 35.0 38.5 27.0 38.5 52-53 35.08401806775685 37.0 35.0 38.5 27.0 40.0 54-55 35.90516400288636 37.0 37.0 40.0 27.0 40.0 56-57 35.924441971198746 37.0 37.0 40.0 27.0 40.0 58-59 35.679547840536934 37.0 37.0 40.0 27.0 40.0 60-61 35.458500497695766 37.0 37.0 40.0 27.0 40.0 62-63 35.201303438184134 37.0 37.0 40.0 27.0 40.0 64-65 34.99782832632587 37.0 33.0 40.0 27.0 40.0 66-67 34.764179200459544 37.0 33.0 37.0 27.0 40.0 68-69 34.532846342399694 37.0 33.0 37.0 27.0 40.0 70-71 34.17861932132682 37.0 33.0 37.0 27.0 40.0 72-73 33.91176976511048 37.0 33.0 37.0 27.0 38.5 74-75 33.67000482506477 37.0 33.0 37.0 27.0 37.0 76-77 33.423067241992186 37.0 33.0 37.0 27.0 37.0 78-79 33.196903673480016 37.0 33.0 37.0 27.0 37.0 80-81 32.99348379539674 37.0 33.0 37.0 27.0 37.0 82-83 32.83792852039369 37.0 33.0 37.0 27.0 37.0 84-85 32.69285839125318 37.0 33.0 37.0 27.0 37.0 86-87 32.569642886027864 35.0 33.0 37.0 27.0 37.0 88-89 32.444923444015245 33.0 33.0 37.0 22.0 37.0 90-91 32.31939699709746 33.0 33.0 37.0 22.0 37.0 92-93 32.20116910175284 33.0 33.0 37.0 22.0 37.0 94-95 32.06662514927914 33.0 33.0 37.0 22.0 37.0 96-97 31.90566801108305 33.0 33.0 37.0 22.0 37.0 98-99 31.765365740304205 33.0 33.0 37.0 22.0 37.0 100-101 30.55363436266733 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.26969242806165994 2306 2 0.3319546943732625 2306 3 0.2816282260811285 2306 4 0.3623481610069277 2306 5 0.2529631620984105 2306 6 0.3488637452288117 2306 7 0.3337873637174198 2306 8 0.33797445080695354 2306 9 0.34700150621475956 2306 10-11 0.46394984302002484 2306 12-13 0.49739248609495945 2306 14-15 0.6990161619453801 2306 16-17 0.683824697513856 2306 18-19 0.6596386429766312 2306 20-21 0.5884892552960679 2306 22-23 0.5564156434659537 2306 24-25 0.538557425072085 2306 26-27 0.6389190729951366 2306 28-29 0.5797028455926778 2306 30-31 0.39335961961617727 2306 32-33 0.4119194501469181 2306 34-35 0.30064588257304337 2306 36-37 0.40921223029736353 2306 38-39 0.47486694183317013 2306 40-41 0.4320866639480059 2306 42-43 0.5347412816503905 2306 44-45 0.5032571830250916 2306 46-47 0.3185920042169954 2306 48-49 0.4882212384929261 2306 50-51 0.6725224616969783 2306 52-53 0.47175770030298025 2306 54-55 0.4495280516186426 2306 56-57 0.47071001130811396 2306 58-59 0.6447678536362318 2306 60-61 0.616611940488653 2306 62-63 0.7287840670774486 2306 64-65 0.5708978585646634 2306 66-67 0.7622294987946532 2306 68-69 0.6851090623627982 2306 70-71 0.7127732960916688 2306 72-73 0.6021729294563869 2306 74-75 0.37075297603779944 2306 76-77 0.6948753368957412 2306 78-79 0.5194492412193839 2306 80-81 0.5837825255120279 2306 82-83 0.5959012468179523 2306 84-85 0.5211799183603105 2306 86-87 0.5778496522352086 2306 88-89 0.4584529687077108 2306 90-91 0.5861998772566892 2306 92-93 0.4836067351740425 2306 94-95 0.6519542916438752 2306 96-97 0.4290861056510451 2306 98-99 0.3119511275559752 2306 100-101 0.35536581828714375 2307 1 0.29109743769240737 2307 2 0.3719568269454321 2307 3 0.32112934089690626 2307 4 0.4478068895838021 2307 5 0.37111244488431794 2307 6 0.3179954465976209 2307 7 0.44288825215245 2307 8 0.2665401183088747 2307 9 0.4101946776093115 2307 10-11 0.4385288308958053 2307 12-13 0.49324593190780774 2307 14-15 0.7208213706161999 2307 16-17 0.5650223145011566 2307 18-19 0.6120915110811396 2307 20-21 0.6015758664338406 2307 22-23 0.5987779592752887 2307 24-25 0.3964067943912042 2307 26-27 0.33265539411675604 2307 28-29 0.43231579331322223 2307 30-31 0.4491181796378285 2307 32-33 0.4145468884219454 2307 34-35 0.46258056298112393 2307 36-37 0.4008545295929338 2307 38-39 0.3826889021317825 2307 40-41 0.44182609370366777 2307 42-43 0.19611137724703553 2307 44-45 0.3843840560317844 2307 46-47 0.19335465303567645 2307 48-49 0.1974204166324185 2307 50-51 0.08665396484594368 2307 52-53 0.18121798182985316 2307 54-55 0.315785018362817 2307 56-57 0.30455666569882567 2307 58-59 0.4603803324643039 2307 60-61 0.4912949596093483 2307 62-63 0.4015610314703295 2307 64-65 0.293854519503455 2307 66-67 0.30833987854287415 2307 68-69 0.3437242438159842 2307 70-71 0.1906136840010788 2307 72-73 0.2634791162396297 2307 74-75 0.3143505432650855 2307 76-77 0.3371572027870329 2307 78-79 0.3051558129592493 2307 80-81 0.38813919273917463 2307 82-83 0.31512658997752396 2307 84-85 0.275049990033871 2307 86-87 0.25294476358691753 2307 88-89 0.31312603582494347 2307 90-91 0.3922537165017559 2307 92-93 0.433776315672489 2307 94-95 0.24725771155143406 2307 96-97 0.23678585315997225 2307 98-99 0.3555998218339873 2307 100-101 0.0556741526799307 2201 1 0.36331848137280076 2201 2 0.3445557292696968 2201 3 0.3376289265941175 2201 4 0.4561700037627503 2201 5 0.4180202230791181 2201 6 0.4478653174475724 2201 7 0.5348598438547967 2201 8 0.3651740976644504 2201 9 0.5263853040314928 2201 10-11 0.4940404177751887 2201 12-13 0.4681357382461897 2201 14-15 0.6059579636149337 2201 16-17 0.6224766822678589 2201 18-19 0.6117148309791034 2201 20-21 0.6254685357223053 2201 22-23 0.5856689477817838 2201 24-25 0.49226666758010396 2201 26-27 0.521424253052551 2201 28-29 0.31348659303544224 2201 30-31 0.3516940188049489 2201 32-33 0.43752392136128293 2201 34-35 0.31736653071808973 2201 36-37 0.2689530339326893 2201 38-39 0.2978968966343416 2201 40-41 0.45178613206974205 2201 42-43 0.32540104288565175 2201 44-45 0.46948973497160296 2201 46-47 0.36814509923161154 2201 48-49 0.28855388377558455 2201 50-51 0.16924798982014266 2201 52-53 0.2666477597744361 2201 54-55 0.4606790311889739 2201 56-57 0.5018726726540308 2201 58-59 0.4934316316706955 2201 60-61 0.44446333334853705 2201 62-63 0.555534349283171 2201 64-65 0.4698665696404376 2201 66-67 0.47377950184463913 2201 68-69 0.49750899493513856 2201 70-71 0.4547149370630166 2201 72-73 0.421134924003141 2201 74-75 0.4794941712520071 2201 76-77 0.5354205760638635 2201 78-79 0.542634712527537 2201 80-81 0.32088026347163634 2201 82-83 0.4071725204725638 2201 84-85 0.4461846901131068 2201 86-87 0.34400162551076363 2201 88-89 0.4906031041205452 2201 90-91 0.42126760335087 2201 92-93 0.29747115311531047 2201 94-95 0.38383212558950675 2201 96-97 0.4048400261952949 2201 98-99 0.28030686353079304 2201 100-101 0.18020477572967053 1101 1 0.29939652375749404 1101 2 0.2288538280630057 1101 3 0.2353147914909819 1101 4 0.37721764614196474 1101 5 0.3673104327754828 1101 6 0.4257544315216819 1101 7 0.37563685280490944 1101 8 0.45098680028118565 1101 9 0.4823025835807755 1101 10-11 0.41131668349984807 1101 12-13 0.39457868919673444 1101 14-15 0.6143891326003654 1101 16-17 0.4460795583190986 1101 18-19 0.4161760110829249 1101 20-21 0.44717037639738066 1101 22-23 0.4866590679941467 1101 24-25 0.5122726522153513 1101 26-27 0.4811251868954969 1101 28-29 0.3408258972987568 1101 30-31 0.43483162372879747 1101 32-33 0.3587321723916048 1101 34-35 0.4693115942791408 1101 36-37 0.3483351089222211 1101 38-39 0.3570599842264457 1101 40-41 0.29214113286636234 1101 42-43 0.31149104883031953 1101 44-45 0.32888592639084635 1101 46-47 0.25445701636497375 1101 48-49 0.16307912460290197 1101 50-51 0.12345296700372899 1101 52-53 0.17992694015351418 1101 54-55 0.40386625490640427 1101 56-57 0.3306524295110975 1101 58-59 0.1961571533418649 1101 60-61 0.36887362121063916 1101 62-63 0.433180394655615 1101 64-65 0.1939524708562672 1101 66-67 0.14587274223338653 1101 68-69 0.37139579852889426 1101 70-71 0.2840307699066713 1101 72-73 0.34516346294695666 1101 74-75 0.49125657780632537 1101 76-77 0.3574237899500403 1101 78-79 0.35272262042354896 1101 80-81 0.5797858347216263 1101 82-83 0.41102370178131054 1101 84-85 0.3742504308744685 1101 86-87 0.3982585114103827 1101 88-89 0.3702719619175028 1101 90-91 0.3277566782507506 1101 92-93 0.3173263797633794 1101 94-95 0.2635839288320554 1101 96-97 0.2370860746514225 1101 98-99 0.3078714481953959 1101 100-101 0.09265435888954698 2304 1 0.23858638787720565 2304 2 0.2618532539493721 2304 3 0.18192676433308108 2304 4 0.3686422652241319 2304 5 0.3196660643862401 2304 6 0.41919349324444966 2304 7 0.32730435524917567 2304 8 0.4106394262204276 2304 9 0.3543438551869684 2304 10-11 0.3396528739481752 2304 12-13 0.39290351845090044 2304 14-15 0.49174473898644777 2304 16-17 0.4799552894563348 2304 18-19 0.508166116955941 2304 20-21 0.5432852213211206 2304 22-23 0.4225813275601027 2304 24-25 0.2952730870887734 2304 26-27 0.3952719924929511 2304 28-29 0.21428176874573523 2304 30-31 0.3411522212912814 2304 32-33 0.22721020623907862 2304 34-35 0.20706526623525434 2304 36-37 0.2426328690718762 2304 38-39 0.266867136532035 2304 40-41 0.37331136613407523 2304 42-43 0.3465813481261435 2304 44-45 0.37925052386050595 2304 46-47 0.3127487205743691 2304 48-49 0.37980323626548085 2304 50-51 0.31096841711789835 2304 52-53 0.36331941694429304 2304 54-55 0.3689522760971542 2304 56-57 0.38756056023142094 2304 58-59 0.4765738246330571 2304 60-61 0.5968184255831801 2304 62-63 0.5511937899676624 2304 64-65 0.46994649967091817 2304 66-67 0.48761892548763086 2304 68-69 0.5327033145743059 2304 70-71 0.3972075858058872 2304 72-73 0.48679270460237944 2304 74-75 0.5655871401248973 2304 76-77 0.5180073577887825 2304 78-79 0.5471758654772358 2304 80-81 0.5526463533611476 2304 82-83 0.6659821163801496 2304 84-85 0.5743686337071026 2304 86-87 0.6205605557190452 2304 88-89 0.6915051862466015 2304 90-91 0.4890422488278716 2304 92-93 0.5087970591042676 2304 94-95 0.5074732510248197 2304 96-97 0.6288537997791366 2304 98-99 0.518827937646936 2304 100-101 0.4489730898259978 2202 1 0.05339247478419651 2202 2 0.05699419247060078 2202 3 0.019533436048966735 2202 4 -0.04386593623536328 2202 5 -0.026741432644357133 2202 6 0.024009516839335276 2202 7 0.1317826158435622 2202 8 0.033152036953310926 2202 9 0.06623201576046966 2202 10-11 0.10516128004858416 2202 12-13 -0.021993180079277863 2202 14-15 0.06982632531221356 2202 16-17 0.089307168460806 2202 18-19 0.109032465339169 2202 20-21 0.07438526119445044 2202 22-23 0.055130098554251106 2202 24-25 -0.014759064742129624 2202 26-27 0.13511631771589805 2202 28-29 -0.053442877404805245 2202 30-31 -0.014826141623565547 2202 32-33 -0.13065855353146816 2202 34-35 -0.11479618553383375 2202 36-37 -0.24884025018314304 2202 38-39 -0.13584467209284412 2202 40-41 -0.03694399197698317 2202 42-43 0.051469839922575034 2202 44-45 0.081445455938713 2202 46-47 -0.056986153402689865 2202 48-49 -0.04403067969672492 2202 50-51 -0.029559661936794157 2202 52-53 0.023332897928504792 2202 54-55 0.059650502472194944 2202 56-57 0.11178614203399206 2202 58-59 0.15374897827197032 2202 60-61 0.13062046397550375 2202 62-63 0.11915841451486386 2202 64-65 0.03584488385154572 2202 66-67 0.1916020394473108 2202 68-69 0.18294983304156887 2202 70-71 0.1430349152840975 2202 72-73 0.138463376183239 2202 74-75 0.006204037557601794 2202 76-77 0.07878212408674301 2202 78-79 0.12727675134035366 2202 80-81 0.10232029919670538 2202 82-83 0.11981575808132305 2202 84-85 0.0863732497311176 2202 86-87 0.11840697376457143 2202 88-89 0.0849157486715697 2202 90-91 0.24135448023666584 2202 92-93 0.07112952253788762 2202 94-95 0.13702941229874455 2202 96-97 0.2115100599979982 2202 98-99 0.2080497958286749 2202 100-101 0.22580773785416497 1102 1 0.1352574061824967 1102 2 0.025001590922670403 1102 3 0.10663045008504568 1102 4 0.2838777497704186 1102 5 0.22824035131554865 1102 6 0.16360254086495019 1102 7 0.26917851145450555 1102 8 0.18572249997785661 1102 9 0.23489268612296854 1102 10-11 0.21356225089171232 1102 12-13 0.2642384065444361 1102 14-15 0.2698243901444073 1102 16-17 0.2760383339308987 1102 18-19 0.18919271760356793 1102 20-21 0.18326707031153688 1102 22-23 0.1257835843516446 1102 24-25 0.16761888982623674 1102 26-27 0.30677751668475395 1102 28-29 0.195534284559578 1102 30-31 0.036228372053493274 1102 32-33 0.13314433638701928 1102 34-35 0.16549484333378217 1102 36-37 0.06460142538698932 1102 38-39 0.053834466900070765 1102 40-41 0.1802173816662389 1102 42-43 0.04240388431124842 1102 44-45 0.17067278417474796 1102 46-47 0.033755290863965115 1102 48-49 0.07505180632168873 1102 50-51 0.07167283625280874 1102 52-53 0.24342299526744426 1102 54-55 0.2100550034688098 1102 56-57 0.4393247002524987 1102 58-59 0.40659759620567826 1102 60-61 0.39636799383565346 1102 62-63 0.37788464632556185 1102 64-65 0.39422186879899357 1102 66-67 0.30997788366426704 1102 68-69 0.31355074338524247 1102 70-71 0.3742133204410152 1102 72-73 0.3912567909090541 1102 74-75 0.28438777053703745 1102 76-77 0.2601347452715359 1102 78-79 0.13575961033689765 1102 80-81 0.12006120467190584 1102 82-83 0.26226834901638796 1102 84-85 0.1064532638272837 1102 86-87 0.21519602112220326 1102 88-89 0.3082697611401599 1102 90-91 0.5260747008050544 1102 92-93 0.3430895521561439 1102 94-95 0.4064638442302382 1102 96-97 0.2304484269404412 1102 98-99 0.20024220854496022 1102 100-101 0.14661018064704123 2305 1 0.08757499804723778 2305 2 0.05696450484013482 2305 3 0.07094220688829367 2305 4 0.10815514198226595 2305 5 0.052800079012676804 2305 6 0.1574786092328111 2305 7 0.07045353773726504 2305 8 0.11825719610684615 2305 9 0.043141533429249534 2305 10-11 0.18510171923995955 2305 12-13 0.08709633740395617 2305 14-15 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sequence quality scores pass #Quality Count 2 1038.0 3 194.0 4 161.0 5 246.0 6 532.0 7 1546.0 8 4907.0 9 8272.0 10 11171.0 11 10471.0 12 8007.0 13 6339.0 14 6003.0 15 6147.0 16 6636.0 17 6693.0 18 7245.0 19 7957.0 20 8586.0 21 9639.0 22 10775.0 23 11863.0 24 13730.0 25 15681.0 26 18206.0 27 21306.0 28 26261.0 29 32566.0 30 40072.0 31 52454.0 32 71804.0 33 103198.0 34 159151.0 35 271247.0 36 525696.0 37 847761.0 38 200992.0 39 128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.654187062826495 23.18561137884365 28.130296677165155 13.0299048811647 2 32.700947827207514 20.312073827588577 35.48300132896616 11.503977016237737 3 40.411289659707386 16.8582604104756 27.78929930828665 14.941150621530369 4 31.773310634046716 15.927951895216552 37.264886132095306 15.033851338641426 5 31.05589767545534 15.126692386916659 38.784935553147406 15.032474384480587 6 23.16044774939692 16.098443531788565 45.803100289921055 14.938008428893463 7 33.76962575118216 16.03491182165451 33.63075754876091 16.56470487840242 8 30.58553720689466 27.037403802513865 26.578965975033586 15.798093015557892 9 30.63116120722038 27.512583605979774 24.24706724857574 17.609187938224103 10-11 25.24633914645606 27.47396021017834 29.587294679532533 17.69240596383307 12-13 26.040733872677162 24.545339788391207 29.322252506234104 20.091673832697527 14-15 24.05229819268304 25.70088141956187 30.842673283831 19.404147103924082 16-17 22.440407386934442 27.624161439642908 31.95729353231473 17.978137641107917 18-19 21.458263305100846 28.232657915556914 31.752186362061796 18.556892417280444 20-21 21.578289971928392 28.30054691750422 31.405841620036988 18.7153214905304 22-23 21.743355567180323 27.380511585830764 32.08842352960692 18.787709317381996 24-25 22.10037840799089 27.4009245744793 31.644596170571386 18.854100846958428 26-27 21.810475712508033 27.798163358358057 31.846659183524846 18.544701745609064 28-29 20.99722977425245 28.45158700245809 31.130409362400467 19.420773860888996 30-31 20.79400910256595 28.761988133950243 30.610369551677564 19.83363321180624 32-33 19.92422281996678 28.906673504062475 31.954953115565445 19.214150560405304 34-35 20.27658012926491 29.16826699419212 32.10624077276378 18.448912103779186 36-37 20.268931376936404 30.127222778151697 31.508005186564787 18.09584065834711 38-39 20.71904471509715 29.909637940149608 30.356311894685735 19.015005450067505 40-41 21.19930774151461 29.051580540759648 29.90423652059358 19.84487519713216 42-43 20.909892483380656 29.311589891192362 30.921636365760452 18.856881259666526 44-45 20.161889345002866 29.504212396540623 31.848338286901345 18.485559971555166 46-47 20.442403910835054 29.20668360981763 31.534730358027335 18.816182121319983 48-49 20.805795328461663 30.248819446300274 29.758504886022504 19.186880339215566 50-51 20.487528566900348 29.793641205473204 29.842761524119737 19.876068703506707 52-53 20.465606504775796 29.43471626336487 30.112184893247367 19.987492338611972 54-55 21.458057112682983 29.26354552423092 29.85839096238689 19.420006400699208 56-57 21.81121035045212 29.389915593773257 28.955433353920217 19.843440701854405 58-59 21.1090481911733 29.290364338492175 30.094236733918294 19.506350736416234 60-61 20.138981644236196 29.589460380555156 30.319927291680393 19.951630683528254 62-63 20.90340740881465 29.67499560676511 29.693360120192576 19.728236864227668 64-65 20.659738060780615 29.75676393984988 29.6055515393644 19.977946460005104 66-67 21.114528129507683 29.325136447627386 29.446838864337728 20.113496558527206 68-69 21.98790471799114 29.231696288102015 29.347840283700855 19.43255871020599 70-71 21.17058120849751 30.645877141448857 29.141691052785816 19.041850597267818 72-73 20.28993134350446 31.210670701668512 28.931901034298967 19.567496920528058 74-75 21.19886205571558 30.13051377258447 28.74510340864887 19.92552076305108 76-77 21.57986254340404 29.319002457394248 29.122011276592673 19.979123722609035 78-79 21.54387075711558 29.2399758609432 29.68785429507029 19.528299086870927 80-81 19.868838497258498 30.020359675060455 29.966101507098774 20.144700320582267 82-83 19.152819373154188 29.74830725978666 30.30082669099349 20.798046676065667 84-85 19.13441943906529 29.047126686121356 30.773585114788464 21.04486876002489 86-87 19.16716210428652 29.133374709961185 31.89663020615219 19.802832979600108 88-89 19.523397436151203 29.742550214841696 31.255606588320244 19.47844576068686 90-91 19.861823632758345 29.56237223346929 30.296815170900633 20.27898896287173 92-93 19.094261051824216 30.590699055919206 30.369386524591828 19.945653367664743 94-95 18.807944949441286 30.882833939507627 30.74531439243963 19.56390671861146 96-97 19.446433309044895 31.62232064656444 29.805317249018753 19.125928795371916 98-99 20.128999676649887 30.52830738287068 29.004208300797135 20.338484639682303 100-101 19.613592210338126 30.522431611591877 28.664484929130467 21.199491248939527 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1202.0 1 878.5 2 481.0 3 496.5 4 530.5 5 502.0 6 629.5 7 838.5 8 1028.0 9 1170.0 10 1293.5 11 1390.0 12 1390.5 13 1318.0 14 1169.5 15 1036.0 16 970.5 17 952.5 18 966.0 19 998.0 20 1119.5 21 1312.5 22 1567.5 23 1924.5 24 2441.0 25 3176.5 26 4423.0 27 6503.5 28 8594.5 29 10530.5 30 12872.5 31 15956.0 32 22820.5 33 33944.5 34 49613.0 35 75291.5 36 119396.0 37 176784.0 38 224183.0 39 231129.5 40 208613.5 41 181224.5 42 156679.5 43 146668.5 44 131856.0 45 112895.5 46 108651.0 47 97801.0 48 74197.5 49 57018.0 50 46395.0 51 39172.0 52 33662.0 53 27799.5 54 22228.5 55 16407.0 56 11693.5 57 8973.0 58 7214.5 59 5602.0 60 4281.5 61 3304.0 62 2510.5 63 1908.0 64 1462.5 65 1041.0 66 721.5 67 519.0 68 402.0 69 323.0 70 232.0 71 195.5 72 153.5 73 113.0 74 89.5 75 71.0 76 68.0 77 49.5 78 30.5 79 33.0 80 32.0 81 26.5 82 23.5 83 13.5 84 8.5 85 12.0 86 10.5 87 11.0 88 13.0 89 10.0 90 8.5 91 8.0 92 4.5 93 3.5 94 4.5 95 3.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1890967739135615 2 0.1931209489478163 3 0.16384704820843332 4 0.165898588421975 5 0.17276335759805672 6 0.17110634434865768 7 0.1382422482355768 8 0.11993619709935886 9 0.11891042699258801 10-11 0.09667883256315095 12-13 0.1437064466889522 14-15 0.12163266304517216 16-17 0.11388020819976953 18-19 0.11693779217187487 20-21 0.1090275265408152 22-23 0.10772558755914453 24-25 0.47370852584605316 26-27 0.32007972600891393 28-29 0.40074076382787416 30-31 0.26482622468073896 32-33 0.29206831155478735 34-35 0.309881203985827 36-37 0.29123980493008783 38-39 0.33793207113636786 40-41 0.3336514535754203 42-43 0.37624063935461705 44-45 0.3309094911746291 46-47 0.35428521380007977 48-49 0.3492944477036755 50-51 0.3649177154837236 52-53 0.35621839592437865 54-55 0.33118566004952893 56-57 0.34722318114192674 58-59 0.33913537837700286 60-61 0.3105913525212837 62-63 0.3183438073666864 64-65 0.3196654726965642 66-67 0.3328623996471351 68-69 0.3186199762415862 70-71 0.30771130568304256 72-73 0.29439602064322884 74-75 0.30252327610456703 76-77 0.2923642067778943 78-79 0.30204984374759586 80-81 0.3105913525212837 82-83 0.33635396327979733 84-85 0.3341248859323915 86-87 0.3272601167563097 88-89 0.34067403353715914 90-91 0.3292130252288158 92-93 0.3131557777882108 94-95 0.3064488193977861 96-97 0.29256147025996565 98-99 0.31593719288541633 100-101 0.3196260200001499 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2534681.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.76736674142697 #Duplication Level Percentage of deduplicated Percentage of total 1 86.57219612757022 36.158926652709695 2 6.2438036380301245 5.215744728221202 3 1.935133795812698 2.4247632883031587 4 1.0529992477345453 1.7592402303430188 5 0.7133966685516816 1.4898350143755155 6 0.5033570575327824 1.2614339294314367 7 0.35662863729277744 1.042680735901194 8 0.34107130611865716 1.1396520262108367 9 0.23344190487969435 0.8775228288534753 >10 1.6396468941886078 13.485658706620182 >50 0.18268337270041013 5.390394398656182 >100 0.19551313156798578 17.74699402264075 >500 0.023961595360997418 6.710610367014818 >1k 0.005783275563167027 4.4378917850265385 >5k 3.8334709571688513E-4 0.8586512856921055 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5518 0.21769997881390205 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5493 0.21671366140354545 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5350 0.21107192581630588 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5090 0.20081422474859753 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4973 0.19619825926812884 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4641 0.18309996405859358 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3888 0.15339208365865367 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3830 0.15110382726662644 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3772 0.1488155708745992 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3678 0.14510701741165852 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 3204 0.12640643931129794 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 2820 0.11125660388822103 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 2708 0.10683790188982362 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2680 0.10573322639022426 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2643 0.10427347662289653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.3397966055689057E-4 0.0 0.0 2 4.7343235697115335E-4 0.0 0.0 3 6.706958390424672E-4 0.0 0.0 4 6.706958390424672E-4 0.0 0.0 5 7.1014853545673E-4 0.0 0.0 6 8.285066246995184E-4 0.0 0.0 7 9.074120175280439E-4 0.0 0.0 8 0.0010257701067708323 0.0 0.0 9 0.0011835808924278834 0.0 0.0 10-11 0.0015978342047776426 0.0 0.0 12-13 0.0021107192581630587 0.0 0.0 14-15 0.002880046838241183 0.0 0.0 16-17 0.0037677325075620954 0.0 0.0 18-19 0.004675144525090139 0.0 0.0 20-21 0.005700914631860971 0.0 0.0 22-23 0.00652942125656049 0.0 0.0 24-25 0.00767354945257411 0.0 0.0 26-27 0.009034667478866176 0.0 0.0 28-29 0.010652228031850952 0.0 0.0 30-31 0.017674807993589725 0.0 0.0 32-33 0.019765800903545655 0.0 0.0 34-35 0.020988834492387802 0.0 0.0 36-37 0.022744479482822495 0.0 0.0 38-39 0.025269452053335313 0.0 0.0 40-41 0.027991688105919444 0.0 0.0 42-43 0.0307928295513321 0.0 0.0 44-45 0.03385041352343747 0.0 0.0 46-47 0.0368882711473357 0.0 0.0 48-49 0.04045874017282648 1.972634820713139E-5 0.0 50-51 0.04434483076963137 3.945269641426278E-5 0.0 52-53 0.04864517467878601 3.945269641426278E-5 0.0 54-55 0.052748255105869335 3.945269641426278E-5 0.0 56-57 0.058251906255659 7.890539282852556E-5 0.0 58-59 0.06491941194966941 7.890539282852556E-5 0.0 60-61 0.07162637034009409 7.890539282852556E-5 0.0 62-63 0.07908292996238975 7.890539282852556E-5 0.0 64-65 0.08713128003089934 7.890539282852556E-5 0.0 66-67 0.09681691700060087 7.890539282852556E-5 0.0 68-69 0.10740996598783042 7.890539282852556E-5 0.0 70-71 0.1201531869296373 7.890539282852556E-5 0.0 72-73 0.13437588398697903 7.890539282852556E-5 0.0 74-75 0.1511235536148336 7.890539282852556E-5 1.972634820713139E-5 76-77 0.17223074619646417 7.890539282852556E-5 3.945269641426278E-5 78-79 0.19527112090239362 7.890539282852556E-5 3.945269641426278E-5 80-81 0.22144798497325702 7.890539282852556E-5 3.945269641426278E-5 82-83 0.25304959480108147 7.890539282852556E-5 3.945269641426278E-5 84-85 0.2897011497699316 7.890539282852556E-5 3.945269641426278E-5 86-87 0.3309883965674576 7.890539282852556E-5 3.945269641426278E-5 88-89 0.3805015305673574 7.890539282852556E-5 3.945269641426278E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 370 0.0 55.06505 9 CGGGAGA 510 0.0 52.040173 4 GGTACGG 55 8.308707E-7 51.71451 1 AGAGCGG 415 0.0 51.37759 8 TTGTGTT 34860 0.0 49.036808 1 ACGTCCC 45 9.728297E-4 42.13275 2 AAGCGTC 115 5.638867E-11 41.20135 9 ATCGGGA 795 0.0 41.139053 2 ACCGGAA 60 8.403753E-5 39.494774 3 TCGGGAA 195 0.0 38.88716 3 TGTGTTT 59635 0.0 37.957363 1 GAACCGG 100 3.0871888E-8 37.923977 1 ATTCGGA 75 7.0271235E-6 37.91948 2 ACTGGCG 315 0.0 37.61853 2 CTCCGGG 165 0.0 37.43669 48-49 TCGGAGA 140 1.0913936E-11 37.23793 4 AAGCGGC 180 0.0 36.85232 9 GAGAGCG 675 0.0 36.501347 7 ACGGCGA 65 1.3443815E-4 36.465363 1 AACGCGT 65 1.3482946E-4 36.44735 8 >>END_MODULE