##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34310-1.34200000035810.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5636509 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.993263738246494 33.0 33.0 33.0 33.0 33.0 2 31.96483674558135 33.0 33.0 33.0 33.0 33.0 3 31.920551000628226 33.0 33.0 33.0 33.0 33.0 4 35.65866319028321 37.0 37.0 37.0 33.0 37.0 5 35.667647119875085 37.0 37.0 37.0 33.0 37.0 6 35.63718056690764 37.0 37.0 37.0 33.0 37.0 7 35.56797709362302 37.0 37.0 37.0 33.0 37.0 8 35.518970518808715 37.0 37.0 37.0 33.0 37.0 9 35.45114413904067 37.0 37.0 37.0 33.0 37.0 10-11 35.36047516290668 37.0 37.0 37.0 33.0 37.0 12-13 35.331634971220666 37.0 37.0 37.0 33.0 37.0 14-15 36.98513583496451 40.0 37.0 40.0 33.0 40.0 16-17 36.91951001941095 40.0 37.0 40.0 33.0 40.0 18-19 36.971233524154755 40.0 37.0 40.0 33.0 40.0 20-21 36.98225213514252 40.0 37.0 40.0 33.0 40.0 22-23 36.95012604432992 40.0 37.0 40.0 33.0 40.0 24-25 36.72560959274615 40.0 37.0 40.0 33.0 40.0 26-27 36.62480038619649 40.0 37.0 40.0 33.0 40.0 28-29 36.57498630801441 40.0 37.0 40.0 33.0 40.0 30-31 36.63194124235409 40.0 37.0 40.0 33.0 40.0 32-33 36.56999625122572 40.0 37.0 40.0 33.0 40.0 34-35 36.51808921089277 40.0 37.0 40.0 33.0 40.0 36-37 36.407811377574305 40.0 37.0 40.0 33.0 40.0 38-39 36.303985143996044 38.5 37.0 40.0 33.0 40.0 40-41 36.16435589830514 37.0 37.0 40.0 33.0 40.0 42-43 36.059718346941345 37.0 37.0 40.0 33.0 40.0 44-45 35.92196384322282 37.0 37.0 40.0 27.0 40.0 46-47 35.83826363091055 37.0 37.0 40.0 27.0 40.0 48-49 35.70458141732764 37.0 37.0 40.0 27.0 40.0 50-51 34.44409749013086 37.0 35.0 38.5 27.0 38.5 52-53 34.91271068670342 37.0 35.0 38.5 27.0 40.0 54-55 35.70878428474079 37.0 37.0 40.0 27.0 40.0 56-57 35.67969615590076 37.0 37.0 40.0 27.0 40.0 58-59 35.464105441861264 37.0 37.0 40.0 27.0 40.0 60-61 35.237125497360154 37.0 35.0 40.0 27.0 40.0 62-63 34.97831122065094 37.0 33.0 40.0 27.0 40.0 64-65 34.759484283623074 37.0 33.0 37.0 27.0 40.0 66-67 34.47497360511622 37.0 33.0 37.0 27.0 40.0 68-69 34.228211646606084 37.0 33.0 37.0 27.0 40.0 70-71 33.91947897182458 37.0 33.0 37.0 27.0 37.0 72-73 33.64511455583589 37.0 33.0 37.0 27.0 37.0 74-75 33.399433408160974 37.0 33.0 37.0 27.0 37.0 76-77 33.15672599830853 37.0 33.0 37.0 27.0 37.0 78-79 32.99677912339003 37.0 33.0 37.0 27.0 37.0 80-81 32.83091644136468 37.0 33.0 37.0 27.0 37.0 82-83 32.610717201019284 37.0 33.0 37.0 24.5 37.0 84-85 32.454997676753464 33.0 33.0 37.0 22.0 37.0 86-87 32.29664984124038 33.0 33.0 37.0 22.0 37.0 88-89 32.184854313192794 33.0 33.0 37.0 22.0 37.0 90-91 32.056439366991164 33.0 33.0 37.0 22.0 37.0 92-93 31.89424713062642 33.0 33.0 37.0 22.0 37.0 94-95 31.721177505438206 33.0 33.0 37.0 22.0 37.0 96-97 31.550524535665605 33.0 33.0 37.0 22.0 37.0 98-99 31.400886967447406 33.0 33.0 37.0 18.5 37.0 100-101 30.08003934704974 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.24649533128586398 2306 2 0.21179108534880697 2306 3 0.25571582802553294 2306 4 0.29603153990982634 2306 5 0.2123507159167346 2306 6 0.2962087747157156 2306 7 0.350986864317413 2306 8 0.3284643150704696 2306 9 0.3440982622991058 2306 10-11 0.3962270149303819 2306 12-13 0.3351929617512255 2306 14-15 0.6445227178647528 2306 16-17 0.5455833580279972 2306 18-19 0.5099531768612167 2306 20-21 0.5359330012357901 2306 22-23 0.47288554827539997 2306 24-25 0.3757468336729701 2306 26-27 0.4832673811993615 2306 28-29 0.3539778702660854 2306 30-31 0.22452509019250755 2306 32-33 0.3138994724603137 2306 34-35 0.29234761521679076 2306 36-37 0.32210222742503447 2306 38-39 0.33393788016336146 2306 40-41 0.28798333020186107 2306 42-43 0.22892863687690834 2306 44-45 0.3410609322053446 2306 46-47 0.3613912743637471 2306 48-49 0.4418535961989747 2306 50-51 0.36327525318458953 2306 52-53 0.3625578167416066 2306 54-55 0.48719601245448274 2306 56-57 0.3826434482566512 2306 58-59 0.4554086715656851 2306 60-61 0.43473755700165384 2306 62-63 0.48849628578199855 2306 64-65 0.456255256158542 2306 66-67 0.3849516710339529 2306 68-69 0.35693306397660507 2306 70-71 0.4468078276367464 2306 72-73 0.4379200904498006 2306 74-75 0.25218306136762436 2306 76-77 0.29371880195768796 2306 78-79 0.3611359282277746 2306 80-81 0.4222988731327959 2306 82-83 0.44621807472180564 2306 84-85 0.39052347307443824 2306 86-87 0.46278287705124654 2306 88-89 0.30992332188453275 2306 90-91 0.4260651673401483 2306 92-93 0.3837012630679588 2306 94-95 0.3186270033497358 2306 96-97 0.3774967732569685 2306 98-99 0.20387782084344863 2306 100-101 0.10310171880093222 2307 1 0.13471834733100962 2307 2 0.1758317736945756 2307 3 0.1510497869773353 2307 4 0.26238734222195603 2307 5 0.19980668710361016 2307 6 0.2600843900762584 2307 7 0.29366904885672795 2307 8 0.31303616221275377 2307 9 0.2995832066000119 2307 10-11 0.2960546420362462 2307 12-13 0.3099841190138264 2307 14-15 0.5388250201609779 2307 16-17 0.48926535287528594 2307 18-19 0.4704672039727882 2307 20-21 0.5107483485220286 2307 22-23 0.3949266133651079 2307 24-25 0.3750589195631946 2307 26-27 0.3660052351343879 2307 28-29 0.34017912835852115 2307 30-31 0.3400329150216237 2307 32-33 0.30353762798849715 2307 34-35 0.261244525058423 2307 36-37 0.2008790887467029 2307 38-39 0.29136340558189744 2307 40-41 0.3641826636528478 2307 42-43 0.23264482513113904 2307 44-45 0.17131808436699458 2307 46-47 0.20673052673954828 2307 48-49 0.2126620327287796 2307 50-51 0.08635608007285356 2307 52-53 0.12967913924748586 2307 54-55 0.20499060965066462 2307 56-57 0.21784510064976814 2307 58-59 0.3564563602996813 2307 60-61 0.31308681922903503 2307 62-63 0.2381948180250859 2307 64-65 0.2639209393395916 2307 66-67 0.29572477230851035 2307 68-69 0.22297356434641813 2307 70-71 0.12836124825128792 2307 72-73 0.24925836704030502 2307 74-75 0.24630673370494094 2307 76-77 0.3007049665842274 2307 78-79 0.20402469490883135 2307 80-81 0.25262374416003297 2307 82-83 0.12526206914747462 2307 84-85 0.18866336916227766 2307 86-87 0.11054874191158603 2307 88-89 0.1583809752185985 2307 90-91 0.17583752370718742 2307 92-93 0.07612075649123895 2307 94-95 0.19292748924910086 2307 96-97 0.11511448428802495 2307 98-99 0.11543143814632018 2307 100-101 -0.11442437903342295 2201 1 0.1318533856659201 2201 2 0.08439698993522882 2201 3 0.0994500653714745 2201 4 0.18661861271788638 2201 5 0.07056850086276256 2201 6 0.2034192741707912 2201 7 0.20114049093928088 2201 8 0.17219117843770704 2201 9 0.12582923625669196 2201 10-11 0.22127202718358063 2201 12-13 0.2130054220153923 2201 14-15 0.31820358743406985 2201 16-17 0.3303440767566883 2201 18-19 0.3456788085881044 2201 20-21 0.27428106145802644 2201 22-23 0.28701859021883536 2201 24-25 0.219705264757998 2201 26-27 0.2983336032460926 2201 28-29 0.222984529650482 2201 30-31 0.21892693906584526 2201 32-33 0.16736686596581052 2201 34-35 0.16080621447810017 2201 36-37 0.08263000724770109 2201 38-39 0.10376976212418043 2201 40-41 0.0949764398074393 2201 42-43 0.06651065225097597 2201 44-45 0.051388585963948685 2201 46-47 0.07178696701829068 2201 48-49 0.02684839847348286 2201 50-51 -0.006663532877574596 2201 52-53 0.035685947739160895 2201 54-55 0.13364648274082214 2201 56-57 0.091266597542905 2201 58-59 0.23912293596360001 2201 60-61 0.35177964593704303 2201 62-63 0.2517152270174634 2201 64-65 0.22872191028324806 2201 66-67 0.3345721527989127 2201 68-69 0.31148726420726547 2201 70-71 0.29188065899917603 2201 72-73 0.2979114102234348 2201 74-75 0.30249559413225313 2201 76-77 0.28623802449494207 2201 78-79 0.1950660890895719 2201 80-81 0.20608024693353144 2201 82-83 0.20234111060990756 2201 84-85 0.22588610675723686 2201 86-87 0.15073347982133356 2201 88-89 0.1301717468894381 2201 90-91 0.20459416145261855 2201 92-93 0.1340066325389948 2201 94-95 0.21289266908864235 2201 96-97 0.17822825955115817 2201 98-99 0.11919371713382887 2201 100-101 0.07043332005483194 1101 1 0.1408591716104013 1101 2 0.08307555566796054 1101 3 0.16788006065675987 1101 4 0.19113551437119725 1101 5 0.18242771659419077 1101 6 0.261762454631949 1101 7 0.27661911061313305 1101 8 0.19686918915984108 1101 9 0.2705316251627252 1101 10-11 0.2543117067470533 1101 12-13 0.22555804693433856 1101 14-15 0.39237437101092354 1101 16-17 0.4238202339815089 1101 18-19 0.24528872583118755 1101 20-21 0.30106873849850047 1101 22-23 0.38450951741700123 1101 24-25 0.2666419407986709 1101 26-27 0.3002542036838136 1101 28-29 0.24034215783401436 1101 30-31 0.24972233042529268 1101 32-33 0.2425055313828821 1101 34-35 0.2435098499001711 1101 36-37 0.15619418414583208 1101 38-39 0.21489649830264312 1101 40-41 0.22329205857344192 1101 42-43 0.21404682287076326 1101 44-45 0.1287510653588697 1101 46-47 0.10899009843573282 1101 48-49 -0.002796356768691055 1101 50-51 0.08858674939991573 1101 52-53 0.1354551585670336 1101 54-55 0.3316543405333121 1101 56-57 0.14420024627168715 1101 58-59 0.012851845714642707 1101 60-61 0.045797184805742575 1101 62-63 0.14672483194391361 1101 64-65 0.15941116407518763 1101 66-67 0.06485865444434324 1101 68-69 0.23172437421944636 1101 70-71 0.31777670658065915 1101 72-73 0.2795749190357668 1101 74-75 0.3263723903992357 1101 76-77 0.17945228609171693 1101 78-79 0.1613489640773409 1101 80-81 0.3010277518386175 1101 82-83 0.24824471056384567 1101 84-85 0.13760280853284002 1101 86-87 0.04104122232186569 1101 88-89 0.1937783428948805 1101 90-91 0.11240477598933296 1101 92-93 0.2867849491121923 1101 94-95 0.15623353221634062 1101 96-97 0.23767304574584358 1101 98-99 0.13915821718606836 1101 100-101 0.059745106907772794 2304 1 0.15594547864345998 2304 2 0.10554053507257777 2304 3 0.11325909775635878 2304 4 0.22830370780855702 2304 5 0.13951537785917623 2304 6 0.19550733746628168 2304 7 0.18701203253837662 2304 8 0.17158918325366557 2304 9 0.1782642453480534 2304 10-11 0.21141282034295017 2304 12-13 0.2546168034100518 2304 14-15 0.26843455701488494 2304 16-17 0.36897005346315837 2304 18-19 0.29643191684895953 2304 20-21 0.2422217465039722 2304 22-23 0.26199893323323664 2304 24-25 0.19398532766035004 2304 26-27 0.25640279583540604 2304 28-29 0.1542035084700757 2304 30-31 0.15107962470150937 2304 32-33 0.1142991476504065 2304 34-35 0.10724092871467406 2304 36-37 0.07731033551935695 2304 38-39 0.07742940141849175 2304 40-41 0.16483245238790545 2304 42-43 0.178782754074156 2304 44-45 0.08527845770211684 2304 46-47 0.10084859166815363 2304 48-49 0.14517730898505476 2304 50-51 0.049371161702005395 2304 52-53 0.06808925994123172 2304 54-55 0.24149576280372997 2304 56-57 0.27718482448280923 2304 58-59 0.24771058154814085 2304 60-61 0.37638773478661847 2304 62-63 0.3020063961166741 2304 64-65 0.2002868520494232 2304 66-67 0.09800033108777484 2304 68-69 0.2182947240333064 2304 70-71 0.17454074057566515 2304 72-73 0.21363822726603843 2304 74-75 0.18821479409294284 2304 76-77 0.17735675268797735 2304 78-79 0.11310874474833099 2304 80-81 0.1877378222443582 2304 82-83 0.17163935622394177 2304 84-85 0.13019344372711572 2304 86-87 0.20514033028081258 2304 88-89 0.358358635680041 2304 90-91 0.1228641010073872 2304 92-93 0.13156793776284204 2304 94-95 0.28816049551746303 2304 96-97 0.348316470274046 2304 98-99 0.11456818270953306 2304 100-101 0.07818554375132791 2202 1 0.18559512824833746 2202 2 0.1302213875110283 2202 3 0.13355647017808892 2202 4 0.17019417659404468 2202 5 0.1353751691180065 2202 6 0.2526928602659453 2202 7 0.22672675161845035 2202 8 0.17078631349748008 2202 9 0.23304277406126772 2202 10-11 0.3008901821989056 2202 12-13 0.2529624220670428 2202 14-15 0.3053741447309548 2202 16-17 0.32812638420673323 2202 18-19 0.3229310916151462 2202 20-21 0.2868586088362832 2202 22-23 0.2840543071429238 2202 24-25 0.19707523316970565 2202 26-27 0.2624948279183954 2202 28-29 0.18215193645059458 2202 30-31 0.18643577484287022 2202 32-33 0.05138476025420147 2202 34-35 0.018822442980209075 2202 36-37 -0.0850211202268909 2202 38-39 -0.08583152951283779 2202 40-41 0.0605051061619406 2202 42-43 0.037048411276849436 2202 44-45 0.034915339913595744 2202 46-47 0.08119617388961586 2202 48-49 0.054724004396994985 2202 50-51 0.1118888355216896 2202 52-53 0.09415537953329078 2202 54-55 0.21643596379201568 2202 56-57 0.20650725061555875 2202 58-59 0.11980592500295728 2202 60-61 0.2162846091358901 2202 62-63 0.26757102402593347 2202 64-65 0.1602716469734844 2202 66-67 0.24222043916085312 2202 68-69 0.2990140714798528 2202 70-71 0.2317757127970168 2202 72-73 0.26993297712899533 2202 74-75 0.2379227691664596 2202 76-77 0.25662914544857784 2202 78-79 0.28599106705220123 2202 80-81 0.2723857895749404 2202 82-83 0.2211401531299444 2202 84-85 0.2590855356463777 2202 86-87 0.3480142461830056 2202 88-89 0.4175784803541873 2202 90-91 0.24717516371273263 2202 92-93 0.17067267710475065 2202 94-95 0.2672811605550329 2202 96-97 0.3082885922307277 2202 98-99 0.21124528096776984 2202 100-101 0.2497032235347625 1102 1 0.1367988985466866 1102 2 0.06295055901386348 1102 3 0.10113763077795213 1102 4 0.1560823266150777 1102 5 0.14472330819956625 1102 6 0.2103747633015658 1102 7 0.25422172343228056 1102 8 0.22420898779859044 1102 9 0.1348705261507277 1102 10-11 0.2533992181046827 1102 12-13 0.3034078245871541 1102 14-15 0.34240163354289166 1102 16-17 0.26764080752953845 1102 18-19 0.2972897661702518 1102 20-21 0.3809924708539967 1102 22-23 0.34200922181823046 1102 24-25 0.19662265363748332 1102 26-27 0.24353519618751562 1102 28-29 0.238368759405013 1102 30-31 0.23729025754760613 1102 32-33 0.26053764658229994 1102 34-35 0.12783618082705317 1102 36-37 0.16032898393008566 1102 38-39 0.0983669522306414 1102 40-41 0.1626194254133182 1102 42-43 0.02177810598862351 1102 44-45 0.07511210221609588 1102 46-47 0.128221554276017 1102 48-49 0.04718989077509406 1102 50-51 0.08800227528013949 1102 52-53 0.17415153531647576 1102 54-55 0.3070015294575015 1102 56-57 0.3315815825973303 1102 58-59 0.3709798845595884 1102 60-61 0.49155414634123673 1102 62-63 0.3944288672834091 1102 64-65 0.3863360073762223 1102 66-67 0.3997956471183457 1102 68-69 0.31079779378823247 1102 70-71 0.3509955801354181 1102 72-73 0.35082501414375145 1102 74-75 0.2912074547154191 1102 76-77 0.28536839252581814 1102 78-79 0.31106490472321013 1102 80-81 0.338535958524659 1102 82-83 0.36690646695378604 1102 84-85 0.3792805070229335 1102 86-87 0.3373878955025944 1102 88-89 0.3641730879180187 1102 90-91 0.3788523658529428 1102 92-93 0.31887274900795504 1102 94-95 0.4266706411275685 1102 96-97 0.2873588469091466 1102 98-99 0.29738147993469966 1102 100-101 0.27323412717857565 2305 1 0.1257719561522208 2305 2 0.06930537530772085 2305 3 0.10493032475351427 2305 4 0.14981283230174824 2305 5 0.10306929322863567 2305 6 0.18168968937050778 2305 7 0.1419019331308391 2305 8 0.2533187364945704 2305 9 0.21674760972229024 2305 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-1.7243544662444137 1213 62-63 -1.1460461539576983 1213 64-65 -1.0075890368598337 1213 66-67 -1.0559495843512323 1213 68-69 -1.1462141090249531 1213 70-71 -1.1510038118623385 1213 72-73 -1.168039034995303 1213 74-75 -1.1599044878517546 1213 76-77 -1.0631989047483046 1213 78-79 -0.9356386318231813 1213 80-81 -1.0338038367812565 1213 82-83 -1.1329957070439853 1213 84-85 -1.2601008896673491 1213 86-87 -1.1848162685332895 1213 88-89 -1.1584746059606488 1213 90-91 -1.027740561054273 1213 92-93 -1.2157143940830402 1213 94-95 -1.3122052562603557 1213 96-97 -1.3368135295221926 1213 98-99 -1.3710951626457266 1213 100-101 -1.1365535397297322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2248.0 3 428.0 4 364.0 5 577.0 6 1083.0 7 4388.0 8 14554.0 9 21471.0 10 26357.0 11 25446.0 12 19454.0 13 15101.0 14 12766.0 15 11734.0 16 12052.0 17 12875.0 18 13921.0 19 15752.0 20 17990.0 21 20248.0 22 22634.0 23 25651.0 24 29411.0 25 34934.0 26 41433.0 27 49455.0 28 59834.0 29 74287.0 30 94268.0 31 125715.0 32 174053.0 33 249935.0 34 380460.0 35 648537.0 36 1244201.0 37 1801432.0 38 331348.0 39 112.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.095173702351296 23.81681625317089 24.90894348132728 15.179066563150531 2 32.071655750080474 21.600336162953713 33.5029256948692 12.825082392096615 3 40.6807641048423 18.741785872945357 24.702318969346955 15.875131052865393 4 31.49573571490259 17.515164094211254 34.238013817300164 16.75108637358599 5 33.99973555984362 16.220986667453353 32.94645335634626 16.83282441635676 6 26.55496869723673 17.092362566483608 39.37990513474076 16.972763601538897 7 34.42104056984137 17.20350297169891 29.790608703067278 18.58484775539245 8 29.95812592418261 25.82469726774986 25.957948303500462 18.25922850456707 9 32.022756103860026 24.82265422040149 24.265741047630517 18.88884862810797 10-11 26.86620544943818 27.691839694160343 27.98319039719164 17.458764459209835 12-13 27.356360700395292 25.118850148300076 27.70464313509089 19.820146016213737 14-15 26.40130632162042 25.713686358473947 27.76659039470651 20.118416925199124 16-17 25.047828485094286 27.061087341301032 28.93696390824284 18.95412026536184 18-19 24.093707391572277 27.235877591629237 29.070198573867366 19.600216442931114 20-21 23.743321538955414 28.121354957790217 28.697537477749563 19.437786025504806 22-23 24.345744589215887 27.50756096950197 28.424485588558586 19.722208852723554 24-25 24.321048607162655 27.17278704712066 28.876577625127215 19.62958672058947 26-27 23.244730538911497 27.185300980241934 29.27989830441512 20.290070176431446 28-29 22.535705512861004 27.836851292031987 28.363970630802886 21.263472564304124 30-31 21.68546949189055 28.70293860593976 28.51026442312396 21.101327479045725 32-33 21.499732466121422 27.551167300046675 29.556087716947555 21.39301251688435 34-35 21.769887265141644 27.83552382296518 29.247214553961115 21.147374357932065 36-37 21.26079169350216 29.168411585316754 29.494245393970825 20.07655132721026 38-39 21.842520158842458 28.506091536294818 28.013340601566135 21.63804770329659 40-41 22.232236697817363 27.828102347268068 27.58105850653253 22.358602448382033 42-43 23.213022191217796 28.08317374503759 27.956191743890308 20.7476123198543 44-45 21.683468346674456 28.943677478948725 28.0190376910538 21.35381648332302 46-47 22.043797972834078 28.66119464280929 28.64710164558667 20.64790573876996 48-49 22.37445893881769 28.111714946063472 28.689778650759752 20.82404746435909 50-51 21.320652929275376 28.536732146512428 27.670019827636942 22.472595096575258 52-53 21.788579292602943 28.668022158173002 27.88229053730062 21.661108011923435 54-55 22.309558664558843 28.582535474730726 27.946005013453977 21.161900847256454 56-57 22.758057522210155 28.36942006421822 27.064954691251273 21.807567722320346 58-59 21.956989586140065 27.70165431995245 27.860491253331464 22.480864840576018 60-61 20.895784970031848 27.72614625640794 28.62982271122692 22.748246062333298 62-63 21.42220314151707 27.79021939429085 28.554479951999213 22.233097512192874 64-65 21.708131053110243 28.513050016869478 28.218414849270694 21.560404080749578 66-67 22.098938052924055 28.04763608207938 27.99182798573983 21.861597879256735 68-69 21.578789938913932 26.912498361358374 29.50641577702406 22.002295922703638 70-71 21.281510591648573 28.808725290580362 28.86572948776577 21.0440346300053 72-73 22.397818674953225 29.414327642018833 27.855348596238215 20.33250508678972 74-75 23.367240307246693 28.52822591408952 27.53940153025396 20.565132248409824 76-77 23.400619029112946 27.208569819964097 27.96555836468694 21.425252786236015 78-79 23.23763645400832 27.325504106366722 28.705255426883646 20.731604012741315 80-81 22.289779061105584 28.672507671001192 28.165435382098664 20.872277885794563 82-83 21.480628488302074 28.660031814463167 28.524636537427124 21.33470315980764 84-85 21.66128246387671 27.906877591409057 28.932439603735492 21.49940034097874 86-87 20.802795784961663 28.465117438188024 29.45984085244206 21.27224592440825 88-89 20.782298171008144 29.087396540663203 29.312774796303685 20.81753049202497 90-91 21.526073126139668 28.105357195148205 28.62870397640841 21.739865702303717 92-93 21.40264712190234 28.570272961642274 27.759957853932193 22.267122062523192 94-95 21.14961904851737 29.722268741334023 27.237839691601128 21.890272518547484 96-97 21.04030163232928 30.54626107374931 26.488570278560424 21.924867015360984 98-99 21.352169320983897 29.0947021515401 27.41160552285938 22.141523004616623 100-101 22.01937779219116 29.078800630553847 27.132344309881635 21.76947726737336 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1185.0 1 1000.5 2 639.0 3 493.0 4 465.0 5 444.0 6 545.5 7 741.5 8 1075.5 9 1424.0 10 1758.5 11 2100.5 12 2281.5 13 2183.0 14 1936.0 15 1686.0 16 1396.0 17 1178.0 18 1085.5 19 1085.0 20 1171.0 21 1314.5 22 1513.5 23 1792.0 24 2160.5 25 2756.5 26 3516.5 27 4371.5 28 5612.5 29 7048.5 30 8873.0 31 11765.5 32 17859.5 33 29279.0 34 47928.5 35 80021.0 36 122671.5 37 184325.5 38 266043.0 39 319804.5 40 338242.0 41 329078.5 42 340160.5 43 383613.0 44 381141.0 45 363924.0 46 379486.5 47 364997.5 48 302130.5 49 245538.5 50 198488.0 51 164495.5 52 147303.5 53 135185.5 54 110279.0 55 70619.0 56 46345.5 57 37815.5 58 32865.0 59 28096.0 60 22403.0 61 17398.0 62 12825.5 63 9137.0 64 6789.5 65 5072.5 66 4147.0 67 3565.5 68 2850.0 69 2122.0 70 1547.0 71 1139.0 72 882.5 73 694.5 74 499.5 75 352.0 76 270.0 77 212.0 78 158.0 79 125.0 80 97.5 81 77.5 82 69.0 83 60.5 84 57.5 85 47.5 86 36.5 87 31.0 88 21.5 89 17.0 90 12.5 91 11.0 92 12.5 93 11.5 94 7.0 95 2.0 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18332269140349106 2 0.18923060355266 3 0.1598329746302188 4 0.1609329462615956 5 0.1690230601955927 6 0.16623764816129982 7 0.13437395380722358 8 0.11592281676477408 9 0.11615345597780469 10-11 0.09443788699707567 12-13 0.1399359071368466 14-15 0.11806953559375138 16-17 0.11060924412610715 18-19 0.11332369024869826 20-21 0.10647547976948143 22-23 0.10552631070047082 24-25 0.4616155141418207 26-27 0.3084533352115645 28-29 0.400957401114768 30-31 0.2679317996298773 32-33 0.29557302223770066 34-35 0.3149112331764218 36-37 0.2948544923817207 38-39 0.3436701688935474 40-41 0.3385961062068738 42-43 0.3821514345138099 44-45 0.3355977964374758 46-47 0.35916734986141247 48-49 0.3548295585086443 50-51 0.37013158321932954 52-53 0.3608971439591421 54-55 0.33752274679238514 56-57 0.35267396894070424 58-59 0.3446725623963343 60-61 0.3157007289441035 62-63 0.32410131874179565 64-65 0.3259907861408542 66-67 0.3378775763508938 68-69 0.32446501903926706 70-71 0.31268467769678004 72-73 0.2981544072758511 74-75 0.30712272436715704 76-77 0.29701008194966066 78-79 0.3070694999333808 80-81 0.31550557268692375 82-83 0.3415678037593837 84-85 0.3397493022720269 86-87 0.3329454454876236 88-89 0.3460918806303689 90-91 0.3355711842205876 92-93 0.31853049467321 94-95 0.3113984205471862 96-97 0.29605204214168734 98-99 0.32138687261920457 100-101 0.32419889687038556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5636509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.03980131969059 #Duplication Level Percentage of deduplicated Percentage of total 1 83.4091529627648 26.72412689170665 2 7.424177718260351 4.75738358110271 3 2.541767224939354 2.4431315066387462 4 1.2665722155328967 1.6232288857085733 5 0.9036207993442985 1.4475915439665659 6 0.6072732618364162 1.167414879359953 7 0.41863709910197106 0.938913463619514 8 0.3462564949251431 0.8875191442443229 9 0.31892222875643833 0.9196384361210277 >10 2.1285159365088746 13.649134093001807 >50 0.3133671187057695 6.912318397469923 >100 0.2667084277145519 17.73086078368413 >500 0.035637903340141504 7.894368233897571 >1k 0.018717049734725787 10.491812834281404 >5k 3.929134327859732E-4 0.8682673444948514 >10k+ 2.80645101643016E-4 1.5442899807021024 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 27675 0.4909954015863365 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23223 0.41201034186231233 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 13721 0.2434308186148554 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10738 0.1905079899632911 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 10515 0.1865516403859197 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8400 0.14902841457363059 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7373 0.1308079167442117 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7319 0.12984987693623837 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6996 0.12411937956632375 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6831 0.12119203570862744 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6270 0.11123906659245997 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.677272936138308E-4 0.0 0.0 2 6.209517273901274E-4 0.0 0.0 3 6.386932053155597E-4 0.0 0.0 4 7.096591170172885E-4 0.0 0.0 5 8.338494624953141E-4 0.0 0.0 6 8.870738962716107E-4 0.0 0.0 7 0.0010822301534513651 0.0 0.0 8 0.0012773864106311193 0.0 0.0 9 0.001419318234034577 0.0 0.0 10-11 0.0021112358731264335 0.0 0.0 12-13 0.002989439030435328 0.0 0.0 14-15 0.004036186228035829 0.0 0.0 16-17 0.005038579730822748 0.0 0.0 18-19 0.00624500022975214 0.0 0.0 20-21 0.007557869596234123 0.0 0.0 22-23 0.00929653443292648 0.0 0.0 24-25 0.01150534843464279 0.0 0.0 26-27 0.013909318693538855 0.0 0.0 28-29 0.016508445209614676 0.0 0.0 30-31 0.02843958911446784 0.0 0.0 32-33 0.03281286342308688 0.0 0.0 34-35 0.03691114482386172 0.0 0.0 36-37 0.0409739432687857 0.0 0.0 38-39 0.04588833265413042 0.0 0.0 40-41 0.05110432716420749 0.0 0.0 42-43 0.05709207596404087 0.0 0.0 44-45 0.06397576939910857 0.0 0.0 46-47 0.07184411485903774 0.0 0.0 48-49 0.08104307116337435 0.0 0.0 50-51 0.09096055732369096 0.0 0.0 52-53 0.10250138871418461 0.0 0.0 54-55 0.11528412355945852 0.0 0.0 56-57 0.13051518235844206 0.0 0.0 58-59 0.14773328668507404 0.0 8.870738962716106E-6 60-61 0.16646828737433045 0.0 1.7741477925432213E-5 62-63 0.18748306797700492 0.0 1.7741477925432213E-5 64-65 0.21134535578671126 0.0 1.7741477925432213E-5 66-67 0.23804628006448672 0.0 1.7741477925432213E-5 68-69 0.26725762346871085 0.0 1.7741477925432213E-5 70-71 0.3022970423714395 0.0 1.7741477925432213E-5 72-73 0.34160328671523454 0.0 1.7741477925432213E-5 74-75 0.3835263990530309 0.0 1.7741477925432213E-5 76-77 0.43133968206207074 0.0 1.7741477925432213E-5 78-79 0.4850786186982049 0.0 1.7741477925432213E-5 80-81 0.5470229888748515 0.0 1.7741477925432213E-5 82-83 0.6148930126785923 0.0 1.7741477925432213E-5 84-85 0.691616033967124 0.0 1.7741477925432213E-5 86-87 0.7751340413010961 0.0 1.7741477925432213E-5 88-89 0.8679485830679947 0.0 1.7741477925432213E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 930 0.0 60.660595 9 CGGGAGA 1455 0.0 57.03294 4 TTGTGTT 70530 0.0 50.03137 1 AGAGCGG 1230 0.0 47.792442 8 CGAGTCG 40 5.4299174E-4 47.428925 1 TCGGGAG 2030 0.0 41.57906 3 GTCGACC 50 0.0016282847 37.943142 1 GATCCGG 420 0.0 37.265587 1 CGAGTGG 260 0.0 36.48379 1 ATGATCG 120 3.8562575E-9 35.571697 1 ATTCGGA 205 0.0 34.701317 2 TGTGTTT 104845 0.0 33.72615 2 AAGCGGC 275 0.0 32.753944 9 TCGGGAA 420 0.0 31.612541 3 CGAGGGG 915 0.0 29.545889 1 CGTTCGA 65 0.005891212 29.184698 2 TGCGGCC 180 2.1464075E-10 28.982027 2 GTGTTTG 148675 0.0 28.813055 1 ATTGACG 150 3.3904143E-8 28.457355 1 TGTTTGA 154270 0.0 27.695147 2 >>END_MODULE