FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5636509
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA276750.4909954015863365No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC232230.41201034186231233No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT137210.2434308186148554No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC107380.1905079899632911No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT105150.1865516403859197No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC84000.14902841457363059No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC73730.1308079167442117No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT73190.12984987693623837No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG69960.12411937956632375No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT68310.12119203570862744No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA62700.11123906659245997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC9300.060.6605959
CGGGAGA14550.057.032944
TTGTGTT705300.050.031371
AGAGCGG12300.047.7924428
CGAGTCG405.4299174E-447.4289251
TCGGGAG20300.041.579063
GTCGACC500.001628284737.9431421
GATCCGG4200.037.2655871
CGAGTGG2600.036.483791
ATGATCG1203.8562575E-935.5716971
ATTCGGA2050.034.7013172
TGTGTTT1048450.033.726152
AAGCGGC2750.032.7539449
TCGGGAA4200.031.6125413
CGAGGGG9150.029.5458891
CGTTCGA650.00589121229.1846982
TGCGGCC1802.1464075E-1028.9820272
GTGTTTG1486750.028.8130551
ATTGACG1503.3904143E-828.4573551
TGTTTGA1542700.027.6951472
Produced by FastQC (version 0.11.2)