##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8142916 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.168696938541427 33.0 33.0 33.0 27.0 33.0 2 30.836215675072665 33.0 33.0 33.0 27.0 33.0 3 30.671700653672467 33.0 33.0 33.0 27.0 33.0 4 34.14650611648211 37.0 37.0 37.0 27.0 37.0 5 34.245361244055566 37.0 37.0 37.0 27.0 37.0 6 34.61555528756529 37.0 37.0 37.0 27.0 37.0 7 34.06770768604269 37.0 37.0 37.0 27.0 37.0 8 34.37966411541025 37.0 37.0 37.0 27.0 37.0 9 33.902920035034136 37.0 37.0 37.0 27.0 37.0 10-11 33.89103755951799 37.0 37.0 37.0 24.5 37.0 12-13 33.856091540180444 37.0 37.0 37.0 24.5 37.0 14-15 35.357973912539435 40.0 37.0 40.0 24.5 40.0 16-17 35.28697815377194 40.0 37.0 40.0 24.5 40.0 18-19 35.33328558221649 40.0 37.0 40.0 24.5 40.0 20-21 35.391563906590704 40.0 37.0 40.0 24.5 40.0 22-23 35.88583893042738 40.0 37.0 40.0 27.0 40.0 24-25 35.30651851253286 40.0 37.0 40.0 22.0 40.0 26-27 35.209200794899516 37.0 37.0 40.0 24.5 40.0 28-29 34.946505711221874 37.0 37.0 40.0 18.5 40.0 30-31 34.9710031394159 37.0 37.0 40.0 18.5 40.0 32-33 34.78922833048996 37.0 37.0 40.0 15.0 40.0 34-35 34.88754501458691 37.0 37.0 40.0 22.0 40.0 36-37 34.74693218006915 37.0 37.0 40.0 22.0 40.0 38-39 34.84504967262342 37.0 37.0 40.0 22.0 40.0 40-41 34.44919620931863 37.0 37.0 40.0 15.0 40.0 42-43 34.354297711041106 37.0 37.0 40.0 15.0 40.0 44-45 34.23133260861343 37.0 33.0 40.0 15.0 40.0 46-47 34.19857886290366 37.0 33.0 40.0 15.0 40.0 48-49 34.100158346223886 37.0 33.0 40.0 15.0 40.0 50-51 33.03600780113659 37.0 33.0 38.5 15.0 38.5 52-53 33.513387403234915 37.0 33.0 38.5 18.5 40.0 54-55 34.20167099844822 37.0 33.0 40.0 18.5 40.0 56-57 34.36559255922571 37.0 33.0 40.0 18.5 40.0 58-59 33.92248317433214 37.0 33.0 40.0 15.0 40.0 60-61 33.77682527978921 37.0 33.0 40.0 15.0 40.0 62-63 33.41967625602426 37.0 33.0 40.0 15.0 40.0 64-65 33.253415913906025 37.0 33.0 38.5 15.0 40.0 66-67 33.099488438785315 37.0 33.0 37.0 15.0 40.0 68-69 33.11371608156095 37.0 33.0 37.0 18.5 40.0 70-71 32.70199367155452 37.0 33.0 37.0 15.0 40.0 72-73 32.83133136827151 37.0 33.0 37.0 22.0 37.0 74-75 32.77836244411707 37.0 33.0 37.0 22.0 37.0 76-77 32.45208141653432 37.0 33.0 37.0 22.0 37.0 78-79 32.04328430994499 37.0 33.0 37.0 22.0 37.0 80-81 31.63487846368549 37.0 33.0 37.0 15.0 37.0 82-83 31.326910408998447 33.0 33.0 37.0 15.0 37.0 84-85 31.14926471057788 33.0 33.0 37.0 15.0 37.0 86-87 30.990121720523703 33.0 33.0 37.0 15.0 37.0 88-89 30.85083224486167 33.0 33.0 37.0 15.0 37.0 90-91 30.705366234896687 33.0 33.0 37.0 15.0 37.0 92-93 30.551867291766243 33.0 27.0 37.0 15.0 37.0 94-95 30.404083868727128 33.0 27.0 37.0 15.0 37.0 96-97 30.2467271552353 33.0 27.0 37.0 15.0 37.0 98-99 30.10664300110673 33.0 27.0 37.0 15.0 37.0 100-101 28.915321243642943 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19121563187247403 2306 2 0.11873857064147941 2306 3 0.15662973125441582 2306 4 0.27357193835205607 2306 5 0.2928744300976902 2306 6 0.16661366041099512 2306 7 0.5320174975158736 2306 8 0.18463647446902343 2306 9 0.28477883096314116 2306 10-11 0.2771984928923672 2306 12-13 0.32362016486764844 2306 14-15 0.6486894866144652 2306 16-17 0.5736208882962757 2306 18-19 0.5819730299181956 2306 20-21 0.4470414172359227 2306 22-23 0.40519662395063705 2306 24-25 0.4062584188881644 2306 26-27 0.4285560646454556 2306 28-29 0.43281339108961703 2306 30-31 0.31241687361887926 2306 32-33 0.39777319102680764 2306 34-35 0.24646263445119843 2306 36-37 0.2955970494772231 2306 38-39 0.24306578114543953 2306 40-41 0.34632875253534223 2306 42-43 0.36053732017255413 2306 44-45 0.34825845658004084 2306 46-47 0.32204021644143666 2306 48-49 0.35848665398177104 2306 50-51 0.4023668421909008 2306 52-53 0.3114654180111174 2306 54-55 0.49927018920909205 2306 56-57 0.39046381550888043 2306 58-59 0.5699273441057713 2306 60-61 0.3569819829411074 2306 62-63 0.5416302073937516 2306 64-65 0.4739620493123837 2306 66-67 0.4448457152559371 2306 68-69 0.29955932574442556 2306 70-71 0.4746704972537188 2306 72-73 0.20900842805006903 2306 74-75 0.1907065679116613 2306 76-77 0.3676640015430479 2306 78-79 0.4143425283200628 2306 80-81 0.48812668812297133 2306 82-83 0.44883618350482024 2306 84-85 0.349054938412948 2306 86-87 0.5037949757282867 2306 88-89 0.42633811004435884 2306 90-91 0.49353541150336966 2306 92-93 0.394687062996951 2306 94-95 0.4222996571775077 2306 96-97 0.4279760205744232 2306 98-99 0.35188840933017573 2306 100-101 0.2615129370834488 2307 1 0.0996381382052185 2307 2 0.01652173674230184 2307 3 -0.011333412523246267 2307 4 0.09968095356426687 2307 5 -0.02938904568820533 2307 6 -0.017335640062107416 2307 7 0.3187406100436547 2307 8 -0.0889757344226112 2307 9 0.10771178145474636 2307 10-11 0.18215972402587255 2307 12-13 0.116266801643782 2307 14-15 0.4240777532619475 2307 16-17 0.41597574674212723 2307 18-19 0.3824066993389579 2307 20-21 0.3212619882339922 2307 22-23 0.1550897768537567 2307 24-25 0.30190002208829725 2307 26-27 0.18014024378886262 2307 28-29 0.2273519573580245 2307 30-31 0.31217246957623956 2307 32-33 0.16084756153139068 2307 34-35 0.09282509920512183 2307 36-37 0.07744760990588873 2307 38-39 0.051873664212997994 2307 40-41 0.2977787701992938 2307 42-43 0.11519758319835205 2307 44-45 0.1494459224189555 2307 46-47 0.05429763021903966 2307 48-49 0.01704041594695127 2307 50-51 -0.012286895281725663 2307 52-53 0.045188227554369576 2307 54-55 0.1771234832778532 2307 56-57 0.14624732022924292 2307 58-59 0.27091581591822234 2307 60-61 0.1628817736911543 2307 62-63 0.1814953314757375 2307 64-65 0.16312011678678573 2307 66-67 0.16697500638512963 2307 68-69 0.06383937650490878 2307 70-71 0.048406568236813996 2307 72-73 0.018486307311341932 2307 74-75 0.07228082951217374 2307 76-77 0.16366166226376322 2307 78-79 0.07742464581860986 2307 80-81 0.12238433865612919 2307 82-83 0.1087849258698732 2307 84-85 0.1496366235110287 2307 86-87 0.22075818944328063 2307 88-89 0.23137444703847976 2307 90-91 0.24722566339191943 2307 92-93 0.18806911373923896 2307 94-95 0.20676934762860455 2307 96-97 0.18928069257961866 2307 98-99 0.20289176962501898 2307 100-101 0.1025486771476416 2201 1 0.20230422786486102 2201 2 0.1093892975638795 2201 3 0.21422384485058643 2201 4 0.26098486722907666 2201 5 0.24350903251588107 2201 6 0.16825550445920356 2201 7 0.43960693212644486 2201 8 0.16814994874779643 2201 9 0.3901771806009364 2201 10-11 0.37021732640926075 2201 12-13 0.31904150752055926 2201 14-15 0.477430365591907 2201 16-17 0.45152560855292734 2201 18-19 0.4752948577459222 2201 20-21 0.37317148223782937 2201 22-23 0.2916217469839637 2201 24-25 0.33215903363488763 2201 26-27 0.34404833592104467 2201 28-29 0.35150101961175295 2201 30-31 0.40401836441567696 2201 32-33 0.33674512191218753 2201 34-35 0.3120269201140715 2201 36-37 0.27269446475759196 2201 38-39 0.23991481427506045 2201 40-41 0.25790282327680103 2201 42-43 0.23009814938478002 2201 44-45 0.25970250551022644 2201 46-47 0.24897650665371884 2201 48-49 0.251800983435146 2201 50-51 0.21255194406082012 2201 52-53 0.21554336391243822 2201 54-55 0.48324706302285136 2201 56-57 0.5113365602978135 2201 58-59 0.5491103572285212 2201 60-61 0.4313475604838999 2201 62-63 0.5719756784956118 2201 64-65 0.5177183629435831 2201 66-67 0.45825910723255703 2201 68-69 0.38302373066144213 2201 70-71 0.40670043316361415 2201 72-73 0.20988483804438118 2201 74-75 0.3456568746851545 2201 76-77 0.42507035584543473 2201 78-79 0.3683854418164145 2201 80-81 0.40029537995058107 2201 82-83 0.3772991427352608 2201 84-85 0.36996505509964805 2201 86-87 0.4291943991332907 2201 88-89 0.4349193996207781 2201 90-91 0.49757028674241965 2201 92-93 0.380417656604255 2201 94-95 0.4374970623800607 2201 96-97 0.4249834193703599 2201 98-99 0.24213650354335314 2201 100-101 0.24325979436479273 1101 1 -0.020316621096071685 1101 2 -0.16869263812674973 1101 3 -0.08198689835241524 1101 4 -0.04576307270826163 1101 5 -0.042188293756190376 1101 6 0.03479062613131134 1101 7 0.2133150302836242 1101 8 0.06301742573261038 1101 9 0.10402291626025573 1101 10-11 0.0749478975781841 1101 12-13 0.04932137385611668 1101 14-15 0.1744977121902238 1101 16-17 0.16841634803896 1101 18-19 0.16944789797549475 1101 20-21 0.2012310649132587 1101 22-23 0.1446709762988334 1101 24-25 -0.012334039491300075 1101 26-27 0.034470324995503177 1101 28-29 0.01806564653224285 1101 30-31 0.13934659155450646 1101 32-33 0.18461318936780202 1101 34-35 0.02916835360451131 1101 36-37 -0.12557974344812806 1101 38-39 0.022322753509975257 1101 40-41 -0.024511792595227178 1101 42-43 -0.06502247275341233 1101 44-45 -0.12105723725814954 1101 46-47 -0.16950449803931633 1101 48-49 -0.1422436208908735 1101 50-51 -0.040381020307521 1101 52-53 -0.1116757856339845 1101 54-55 0.1199692256532856 1101 56-57 0.10088361723617112 1101 58-59 -0.052587904870271984 1101 60-61 0.04033634446523848 1101 62-63 0.13247762624443027 1101 64-65 0.09561962700476556 1101 66-67 0.013105028740184821 1101 68-69 0.07162647331421823 1101 70-71 0.10576400279746423 1101 72-73 -0.05595434024268542 1101 74-75 0.04766063408914789 1101 76-77 0.0768479693189974 1101 78-79 0.05717019142606716 1101 80-81 0.1674822328655452 1101 82-83 0.16434697891948602 1101 84-85 0.06333994699300405 1101 86-87 0.0370347100706816 1101 88-89 0.05079847310110708 1101 90-91 -0.06862436302132124 1101 92-93 0.10233937934491877 1101 94-95 0.12572380975660025 1101 96-97 0.18064612224867105 1101 98-99 -0.02149005549714289 1101 100-101 -0.1791917972723276 2304 1 0.15775667614298428 2304 2 7.611262421036713E-4 2304 3 0.04520431340468889 2304 4 0.11934471043883832 2304 5 0.07672261695861948 2304 6 0.16859857516736554 2304 7 0.2410861253658254 2304 8 0.09019684027994401 2304 9 0.18313466578480586 2304 10-11 0.20497788532431116 2304 12-13 0.11444404001890263 2304 14-15 0.1623501021262328 2304 16-17 0.21811515518055558 2304 18-19 0.25693473573093684 2304 20-21 0.24026338678614678 2304 22-23 0.23499197788028425 2304 24-25 0.0702897997741232 2304 26-27 0.15068268602255586 2304 28-29 0.13732792961167917 2304 30-31 0.22329670689581604 2304 32-33 0.12806225917518788 2304 34-35 0.046964643092486824 2304 36-37 0.07103264971101453 2304 38-39 -0.0054780827847977775 2304 40-41 0.17778082056450017 2304 42-43 0.042008581539896284 2304 44-45 0.09107814540329429 2304 46-47 0.06109564819313107 2304 48-49 0.0628344284893032 2304 50-51 -0.06191814627558756 2304 52-53 -0.05315405543831986 2304 54-55 0.134597269391314 2304 56-57 0.20989618498739304 2304 58-59 0.1393899883499259 2304 60-61 0.23763013817323753 2304 62-63 0.28578844781007007 2304 64-65 0.2104023209116832 2304 66-67 0.1586916147997499 2304 68-69 0.11186517570717314 2304 70-71 0.1456117881815615 2304 72-73 0.08664679063486602 2304 74-75 0.1654024131877989 2304 76-77 0.2514894656817219 2304 78-79 0.1489443139072435 2304 80-81 0.10448393311979132 2304 82-83 0.08719390954362183 2304 84-85 0.11892788650525077 2304 86-87 0.2200675303053643 2304 88-89 0.22078679336705775 2304 90-91 0.12489289647857404 2304 92-93 0.09115114055086337 2304 94-95 0.16171226950428874 2304 96-97 0.177336057656877 2304 98-99 0.0677644816303129 2304 100-101 0.043773295727081774 2202 1 0.22468101849282007 2202 2 0.13408989636627666 2202 3 0.1587563241013008 2202 4 0.22466895608747706 2202 5 0.1615474886049526 2202 6 0.21782075751473684 2202 7 0.300743314405473 2202 8 0.2634260838996596 2202 9 0.2736418825792981 2202 10-11 0.3344467132147315 2202 12-13 0.30468339311425297 2202 14-15 0.39161771318811844 2202 16-17 0.4050210150003011 2202 18-19 0.3537763754079819 2202 20-21 0.3589587806174066 2202 22-23 0.2829631612910859 2202 24-25 0.24393868106481165 2202 26-27 0.32102231448943286 2202 28-29 0.2827720392163826 2202 30-31 0.3013918925772927 2202 32-33 0.2589157246668279 2202 34-35 0.18004803852481643 2202 36-37 0.11140605154665195 2202 38-39 0.14157708748704323 2202 40-41 0.29932029965655005 2202 42-43 0.1792703218517957 2202 44-45 0.31783546126745676 2202 46-47 0.31861944369370576 2202 48-49 0.21073119275498442 2202 50-51 0.13554329013579292 2202 52-53 0.19472556613315817 2202 54-55 0.33887080930363567 2202 56-57 0.4015447802215988 2202 58-59 0.3992466351086321 2202 60-61 0.36563309899591445 2202 62-63 0.488371249744155 2202 64-65 0.37140979701194254 2202 66-67 0.4497878803214945 2202 68-69 0.4309558862754912 2202 70-71 0.399206081891073 2202 72-73 0.35844077722455125 2202 74-75 0.3392121054440551 2202 76-77 0.3824131422526946 2202 78-79 0.3946552869331583 2202 80-81 0.39277259861412617 2202 82-83 0.4377708207988107 2202 84-85 0.400061307178305 2202 86-87 0.46057885935357135 2202 88-89 0.4717158254490741 2202 90-91 0.5367017063440578 2202 92-93 0.43773732610709004 2202 94-95 0.5103143791785918 2202 96-97 0.46192152945156906 2202 98-99 0.41495027823556185 2202 100-101 0.4743244396694486 1102 1 0.15305155608590226 1102 2 0.01838366550152415 1102 3 0.0621741530742419 1102 4 0.121877183375247 1102 5 0.193032373542124 1102 6 0.1769081553714429 1102 7 0.24503119804338525 1102 8 0.24960624671420817 1102 9 0.13694686681716206 1102 10-11 0.19223869510686598 1102 12-13 0.2788969798190095 1102 14-15 0.3315619831064751 1102 16-17 0.29216703936891264 1102 18-19 0.2716656387652776 1102 20-21 0.2139569211218486 1102 22-23 0.200981730205946 1102 24-25 0.11655168215119716 1102 26-27 0.2936935995734089 1102 28-29 0.1407116210145105 1102 30-31 0.13095240416304677 1102 32-33 0.12232502315496419 1102 34-35 0.11652371954594543 1102 36-37 0.048930433635241855 1102 38-39 0.009403177088472603 1102 40-41 0.14557825961026793 1102 42-43 0.13502315003177756 1102 44-45 0.06107718032219367 1102 46-47 0.08054811483848567 1102 48-49 0.08549034236607866 1102 50-51 0.05186139180391791 1102 52-53 0.10090959486280582 1102 54-55 0.3535163023623866 1102 56-57 0.30106918586071885 1102 58-59 0.29957959402889855 1102 60-61 0.3380108207093713 1102 62-63 0.37613269172515373 1102 64-65 0.32982665408094647 1102 66-67 0.32595811384952356 1102 68-69 0.21905931428597825 1102 70-71 0.3668265970898048 1102 72-73 0.3226665123727557 1102 74-75 0.2190198459829915 1102 76-77 0.1928744163276832 1102 78-79 0.16945193773511846 1102 80-81 0.2292391155630007 1102 82-83 0.37242709018041253 1102 84-85 0.2798292394996409 1102 86-87 0.3481820378778835 1102 88-89 0.3667405417630505 1102 90-91 0.41657837308638435 1102 92-93 0.4082345097557436 1102 94-95 0.3809936311711546 1102 96-97 0.23655281962131625 1102 98-99 0.1754655086319339 1102 100-101 0.16565236532263938 2305 1 0.1520807872227259 2305 2 0.11238062869197663 2305 3 0.04141571161121149 2305 4 0.05939570425647389 2305 5 0.05002619447597567 2305 6 0.2520233230691531 2305 7 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-1.3390510741213468 1213 88-89 -1.3173329062442996 1213 90-91 -1.222768036263279 1213 92-93 -1.26648451190702 1213 94-95 -1.4159360101105065 1213 96-97 -1.3460532928080617 1213 98-99 -1.4057115123227035 1213 100-101 -1.3706924955473667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3196.0 3 763.0 4 995.0 5 1134.0 6 2039.0 7 10069.0 8 44461.0 9 119655.0 10 156523.0 11 122197.0 12 79091.0 13 54101.0 14 39504.0 15 32261.0 16 28336.0 17 27439.0 18 27853.0 19 29390.0 20 31180.0 21 33647.0 22 36021.0 23 39254.0 24 43953.0 25 50062.0 26 58669.0 27 69109.0 28 84698.0 29 103461.0 30 126725.0 31 164886.0 32 224901.0 33 319960.0 34 484845.0 35 824150.0 36 1604319.0 37 2470164.0 38 593644.0 39 261.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.764899653979235 23.703498654363706 25.66639907727797 13.865202614379085 2 29.215537281271065 25.360240971860122 33.541763198049544 11.882458548819267 3 36.58930993197399 19.84257070554626 28.60103275152193 14.967086610957814 4 26.74343459887794 18.08061555386416 36.657895086422656 18.51805476083524 5 34.16962608602592 16.984196885136807 33.05050428013841 15.79567274869886 6 27.409413250347527 17.60369504807319 40.182270111517404 14.804621590061876 7 33.45625979973766 19.50738499499461 31.138741351852563 15.897613853415168 8 26.621718615643637 30.367388168168773 27.42367974372259 15.587213472465006 9 33.297629862461726 26.073603000271834 23.890761149066538 16.738005988199905 10-11 26.521461358123826 29.29651389870968 27.350224374959858 16.831800368206636 12-13 28.238650393962615 24.15810719875805 27.104722779982037 20.498519627297295 14-15 25.18851422719771 24.816532818522376 28.816547080576054 21.17840587370386 16-17 25.77151236357925 26.291202838430568 29.93675677220728 18.000528025782902 18-19 25.1929916229578 26.710184460901758 30.067720187426904 18.02910372871354 20-21 24.29145019581492 28.728335857776543 28.886914164136385 18.09329978227215 22-23 27.020390967690304 26.710532049969583 28.192551522934668 18.076525459405445 24-25 23.75157281315282 29.60961170652895 28.42748663625432 18.211328844063914 26-27 22.579180690638758 30.593081984089 28.374753259493744 18.452984065778498 28-29 23.073428172150436 29.593592224403082 28.106397856611416 19.226581746835063 30-31 24.92698476492077 27.54199495740986 28.37553130323306 19.15548897443631 32-33 22.31406241224479 27.034208890163235 30.01340899618385 20.638319701408122 34-35 20.195235573960606 27.256331876330602 33.43018077559587 19.118251774112917 36-37 21.45340409727509 27.94427678290019 29.77720344649677 20.825115673327954 38-39 20.533151158012878 27.56448515293935 27.80390448389235 24.09845920515542 40-41 22.21775486102259 27.047630853140785 28.8891030155489 21.845511270287723 42-43 23.69960690936184 27.22425100996977 30.345135256808202 18.731006823860188 44-45 21.531088451748325 29.322036331277886 30.591821645893962 18.55505357107983 46-47 23.093140164742234 29.669241800092767 28.748672733133567 18.488945302031436 48-49 20.959883889595048 28.811052671404447 30.20799211667829 20.021071322322218 50-51 20.211137842265618 27.970870772579392 30.409381088348788 21.408610296806206 52-53 24.26599557925812 27.561974225012914 28.00104118953439 20.170989006194574 54-55 25.186257168891036 27.49080034673128 28.024956273538205 19.29798621083948 56-57 25.46539884036249 27.138301551718687 27.443544555354705 19.952755052564118 58-59 23.903672424433015 26.836101313291234 27.964566176195145 21.295660086080613 60-61 20.915812517242674 27.114994945844995 27.921873499591044 24.04731903732129 62-63 23.32280812656268 27.305072950900307 28.301073438034287 21.071045484502726 64-65 21.041387447202535 28.649963713918204 30.07039703152069 20.238251807358576 66-67 20.50035156932748 28.56082309547721 28.536641730185618 22.40218360500969 68-69 20.239493197390587 27.23480055097739 32.436622415707276 20.089083835924743 70-71 19.84078301118796 31.43061297659911 29.556174128437306 19.172429883775628 72-73 19.819840569928022 34.15565124425416 27.080011873500414 18.9444963123174 74-75 20.630062373217406 33.53503991265089 26.745929037716117 19.08896867641559 76-77 20.82021122683492 32.54736690675305 27.31183655215611 19.32058531425591 78-79 20.75727676976707 32.2881391424313 27.81680277890691 19.13778130889472 80-81 20.0201969246348 32.80176432055273 27.570828594385823 19.607210160426654 82-83 19.324978935126598 32.22005775188573 28.070308497196567 20.3846548157911 84-85 19.466259895351143 31.457456127947893 28.410860715264207 20.665423261436764 86-87 19.413170037517787 31.28209803909489 29.082791717747945 20.221940205639374 88-89 19.676000175968323 31.558125552904663 28.68070461757882 20.085169653548196 90-91 20.226710523308405 30.764256257879126 28.256342403639433 20.75269081517304 92-93 19.81721238351792 31.569743816578182 27.93181147475398 20.681232325149917 94-95 19.56133011673949 32.11964566223005 27.781653983589855 20.537370237440598 96-97 20.094053545476754 32.31622328513443 27.313254051542792 20.276469117846027 98-99 20.484873736367323 31.259953110236648 27.16931065543108 21.08586249796495 100-101 20.52055926280562 31.329738661457405 26.818351947337067 21.33135012839991 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1554.0 1 1374.0 2 947.0 3 740.5 4 786.0 5 820.0 6 977.5 7 1397.5 8 1946.5 9 2503.0 10 2980.0 11 3253.0 12 3337.0 13 3187.0 14 2922.0 15 2721.5 16 2591.5 17 2489.5 18 2493.0 19 2775.0 20 3265.0 21 3967.5 22 5050.0 23 6668.0 24 8694.5 25 11308.5 26 15117.5 27 19934.0 28 25519.5 29 31765.0 30 38557.5 31 46598.5 32 58478.0 33 82272.0 34 121529.0 35 196588.5 36 296160.5 37 397836.5 38 515816.5 39 578239.0 40 574344.5 41 525412.0 42 471201.0 43 474665.0 44 466238.5 45 441276.5 46 444890.5 47 405028.5 48 320601.0 49 270690.0 50 231897.0 51 196584.5 52 174597.0 53 150877.5 54 118880.5 55 86832.0 56 65825.5 57 51248.5 58 40639.5 59 31611.0 60 23947.5 61 18296.5 62 13635.0 63 9979.0 64 7603.5 65 5659.0 66 4066.0 67 3010.5 68 2244.5 69 1590.5 70 1116.5 71 847.5 72 641.5 73 485.0 74 381.5 75 296.5 76 242.0 77 193.5 78 147.0 79 130.0 80 117.5 81 97.0 82 86.5 83 71.5 84 55.0 85 47.0 86 43.5 87 39.5 88 32.0 89 21.0 90 15.5 91 12.5 92 9.5 93 9.0 94 7.5 95 5.5 96 4.0 97 1.5 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18164254672404823 2 0.18619865414306128 3 0.15706904013255202 4 0.1580760503976708 5 0.16586195903285753 6 0.16373741298571667 7 0.13119378856419492 8 0.11335005789080964 9 0.11363251198956245 10-11 0.09257740101948737 12-13 0.13729725321985392 14-15 0.11599038968349913 16-17 0.10860360097046318 18-19 0.11148954502293773 20-21 0.10375889914620268 22-23 0.10324925370714863 24-25 0.45704757362104687 26-27 0.30483551592574454 28-29 0.39552784285138154 30-31 0.26396563589750893 32-33 0.2915417523648776 34-35 0.3111661719217047 36-37 0.2912347370401463 38-39 0.3396387731372889 40-41 0.334800211619523 42-43 0.37814463516509317 44-45 0.3317791808241667 46-47 0.3557386567662002 48-49 0.35085711310297196 50-51 0.36629998393695823 52-53 0.3576053099405667 54-55 0.33306250488154365 56-57 0.34873870736232576 58-59 0.3405659594179775 60-61 0.3111600316152101 62-63 0.31994066990252634 64-65 0.3213529403962905 66-67 0.33357215032059767 68-69 0.3201248790973651 70-71 0.30856882227447757 72-73 0.29468558929012656 74-75 0.30318377347868997 76-77 0.29257946416246955 78-79 0.3022995693434637 80-81 0.3112337152931456 82-83 0.33783966333436327 84-85 0.3352545943001254 86-87 0.3287028872703587 88-89 0.3416343727480426 90-91 0.3306862062681231 92-93 0.31470912876910434 94-95 0.3071626920872081 96-97 0.29249349987154477 98-99 0.3167477105253204 100-101 0.32037663166364483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8142916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.77303661957887 #Duplication Level Percentage of deduplicated Percentage of total 1 90.93772176983161 37.9874478159225 2 4.230105037720542 3.5340866529073054 3 1.221348404864659 1.5305829492502694 4 0.8217365689652318 1.3730572714812692 5 0.43514508371505745 0.9088665758429404 6 0.3501779586270483 0.8776798013458237 7 0.22072424476741373 0.6454225371648645 8 0.20275167970400929 0.6775642672765364 9 0.15215348462061876 0.5720321776368273 >10 1.0792418506867192 9.124027609284537 >50 0.15447129921229236 4.463271406432584 >100 0.1479304125826557 13.737467991375176 >500 0.025956362196610246 7.491629641681792 >1k 0.019939207905419865 14.819998526263944 >5k 3.8795355477161386E-4 1.0448446999231547 >10k+ 2.0868104525425719E-4 1.2120200762104678 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 16887 0.20738271154952354 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16748 0.2056757063440173 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 15715 0.19298983312611845 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 13032 0.16004094847595138 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 12837 0.15764622894304695 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11557 0.14192704431680248 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10550 0.12956046703662422 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 9903 0.12161491043257723 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9318 0.11443075183386393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002099984821162345 0.0 0.0 2 0.0023087552419796546 0.0 0.0 3 0.002419280758882936 0.0 0.0 4 0.0026157705667109914 0.0 0.0 5 0.0029719083433993425 0.0 0.0 6 0.0032543624421521723 0.0 0.0 7 0.003770148187700819 0.0 0.0 8 0.004371898224174239 0.0 0.0 9 0.004826280904776618 0.0 0.0 10-11 0.007380648406541342 0.0 0.0 12-13 0.00998413836026308 0.0 0.0 14-15 0.012876222719232274 0.0 0.0 16-17 0.015252521332652823 0.0 1.228061298925348E-5 18-19 0.018543725613772755 0.0 1.228061298925348E-5 20-21 0.022448960544355363 0.0 1.228061298925348E-5 22-23 0.026329634248959462 0.0 1.228061298925348E-5 24-25 0.031057670249822054 0.0 1.228061298925348E-5 26-27 0.036166405253351505 0.0 1.842091948388022E-5 28-29 0.041698821405010196 0.0 3.6841838967760446E-5 30-31 0.06622320554454939 0.0 3.6841838967760446E-5 32-33 0.07370823916149939 0.0 3.6841838967760446E-5 34-35 0.08019854312631985 0.0 3.6841838967760446E-5 36-37 0.08704498486782868 0.0 4.298214546238719E-5 38-39 0.0944870363393163 0.0 4.912245195701392E-5 40-41 0.1025983812187182 6.14030649462674E-6 4.912245195701392E-5 42-43 0.11110884602027088 1.842091948388022E-5 6.140306494626741E-5 44-45 0.11965001235429668 2.456122597850696E-5 6.140306494626741E-5 46-47 0.12934555630931227 3.0701532473133704E-5 6.140306494626741E-5 48-49 0.13967355183327446 3.6841838967760446E-5 6.140306494626741E-5 50-51 0.15128487141461364 3.6841838967760446E-5 6.140306494626741E-5 52-53 0.16308040019079162 3.6841838967760446E-5 6.754337144089414E-5 54-55 0.1757110106502388 3.6841838967760446E-5 7.368367793552089E-5 56-57 0.18873460072534212 3.6841838967760446E-5 7.368367793552089E-5 58-59 0.20372308887872598 3.6841838967760446E-5 7.368367793552089E-5 60-61 0.2199580592505191 3.6841838967760446E-5 7.368367793552089E-5 62-63 0.2373412669368074 3.6841838967760446E-5 7.982398443014763E-5 64-65 0.2559218343895479 3.6841838967760446E-5 8.596429092477436E-5 66-67 0.2764120371621174 3.6841838967760446E-5 8.596429092477436E-5 68-69 0.2989039798519351 3.6841838967760446E-5 9.210459741940111E-5 70-71 0.32342222368497964 3.6841838967760446E-5 9.824490391402785E-5 72-73 0.3511764090406926 3.6841838967760446E-5 1.1052551690328133E-4 74-75 0.38117794657343884 3.6841838967760446E-5 1.1052551690328133E-4 76-77 0.4144277062418426 3.6841838967760446E-5 1.1052551690328133E-4 78-79 0.45120814214465677 3.6841838967760446E-5 1.1052551690328133E-4 80-81 0.4938832722823126 3.6841838967760446E-5 1.2280612989253481E-4 82-83 0.5419987139742077 3.6841838967760446E-5 1.2280612989253481E-4 84-85 0.5937921992563844 3.6841838967760446E-5 1.2280612989253481E-4 86-87 0.651062837931768 3.6841838967760446E-5 1.2280612989253481E-4 88-89 0.7155053545928756 3.6841838967760446E-5 1.2280612989253481E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 6130 0.0 84.91132 9 CGGGAGA 8020 0.0 84.84041 4 AGAGCGG 6455 0.0 82.839325 8 GAGAGCG 7550 0.0 77.04089 7 AAGCGGC 2115 0.0 74.63759 9 TCGGGAG 8850 0.0 74.41907 3 TCGGGAA 2690 0.0 72.79859 3 ATTCGGA 1140 0.0 72.798065 2 CGGAAAG 2895 0.0 66.98846 5 GAACCGG 895 0.0 66.23777 1 GATCGGG 12760 0.0 63.92879 1 GATCCGG 1540 0.0 63.748264 1 ATCGGGA 12330 0.0 63.576477 2 AGAGCGT 19475 0.0 63.409454 8 TCGGAGA 1680 0.0 63.22138 4 ACCGGAA 1035 0.0 63.221375 3 AACCGGA 930 0.0 62.7204 2 GTTCGGA 825 0.0 62.6555 2 GAGCGTC 19955 0.0 61.36156 9 AAGAGCG 20080 0.0 60.743504 7 >>END_MODULE