FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8142916
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT168870.20738271154952354Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC167480.2056757063440173No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG157150.19298983312611845No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA130320.16004094847595138No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA128370.15764622894304695No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC115570.14192704431680248No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG105500.12956046703662422No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC99030.12161491043257723No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA93180.11443075183386393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC61300.084.911329
CGGGAGA80200.084.840414
AGAGCGG64550.082.8393258
GAGAGCG75500.077.040897
AAGCGGC21150.074.637599
TCGGGAG88500.074.419073
TCGGGAA26900.072.798593
ATTCGGA11400.072.7980652
CGGAAAG28950.066.988465
GAACCGG8950.066.237771
GATCGGG127600.063.928791
GATCCGG15400.063.7482641
ATCGGGA123300.063.5764772
AGAGCGT194750.063.4094548
TCGGAGA16800.063.221384
ACCGGAA10350.063.2213753
AACCGGA9300.062.72042
GTTCGGA8250.062.65552
GAGCGTC199550.061.361569
AAGAGCG200800.060.7435047
Produced by FastQC (version 0.11.2)