##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34298-1.342000000357ce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5191010 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73806330559949 33.0 33.0 33.0 27.0 33.0 2 31.617736047512913 33.0 33.0 33.0 27.0 33.0 3 31.556089084783114 33.0 33.0 33.0 27.0 33.0 4 35.22336982591057 37.0 37.0 37.0 33.0 37.0 5 35.272357595150076 37.0 37.0 37.0 33.0 37.0 6 35.36793629756059 37.0 37.0 37.0 33.0 37.0 7 35.15167221793062 37.0 37.0 37.0 33.0 37.0 8 35.19209363881017 37.0 37.0 37.0 33.0 37.0 9 35.01439353805907 37.0 37.0 37.0 33.0 37.0 10-11 34.94120556500565 37.0 37.0 37.0 33.0 37.0 12-13 34.904664699162595 37.0 37.0 37.0 33.0 37.0 14-15 36.4734270402099 40.0 37.0 40.0 33.0 40.0 16-17 36.41979017570762 40.0 37.0 40.0 33.0 40.0 18-19 36.43683252392116 40.0 37.0 40.0 33.0 40.0 20-21 36.45253833069094 40.0 37.0 40.0 33.0 40.0 22-23 36.55541532380018 40.0 37.0 40.0 33.0 40.0 24-25 36.20483691998282 40.0 37.0 40.0 30.0 40.0 26-27 36.099020614485426 40.0 37.0 40.0 27.0 40.0 28-29 35.98093646515804 40.0 37.0 40.0 27.0 40.0 30-31 35.999871412307044 40.0 37.0 40.0 27.0 40.0 32-33 35.86634229176981 37.0 37.0 40.0 27.0 40.0 34-35 35.86128065636552 37.0 37.0 40.0 27.0 40.0 36-37 35.75346368047836 37.0 37.0 40.0 27.0 40.0 38-39 35.72613422050814 37.0 37.0 40.0 27.0 40.0 40-41 35.462273815692896 37.0 37.0 40.0 27.0 40.0 42-43 35.37846604032741 37.0 37.0 40.0 27.0 40.0 44-45 35.26010785954949 37.0 37.0 40.0 27.0 40.0 46-47 35.18882259907032 37.0 37.0 40.0 27.0 40.0 48-49 35.06499640725023 37.0 37.0 40.0 27.0 40.0 50-51 33.887282917967795 37.0 33.0 38.5 24.5 38.5 52-53 34.38732106083402 37.0 33.0 38.5 27.0 40.0 54-55 35.16258839801888 37.0 37.0 40.0 27.0 40.0 56-57 35.210176824933875 37.0 37.0 40.0 27.0 40.0 58-59 34.881764820333615 37.0 33.0 40.0 27.0 40.0 60-61 34.670608609885164 37.0 33.0 40.0 27.0 40.0 62-63 34.37327456506537 37.0 33.0 40.0 27.0 40.0 64-65 34.164207350785304 37.0 33.0 38.5 27.0 40.0 66-67 33.93927395632063 37.0 33.0 37.0 27.0 40.0 68-69 33.77111910784221 37.0 33.0 37.0 27.0 40.0 70-71 33.398050090444826 37.0 33.0 37.0 22.0 40.0 72-73 33.24530862009512 37.0 33.0 37.0 22.0 37.0 74-75 33.053520605816594 37.0 33.0 37.0 24.5 37.0 76-77 32.77160494778472 37.0 33.0 37.0 22.0 37.0 78-79 32.49100020997841 37.0 33.0 37.0 22.0 37.0 80-81 32.21762335268088 37.0 33.0 37.0 22.0 37.0 82-83 32.00386321737003 33.0 33.0 37.0 22.0 37.0 84-85 31.839919495435375 33.0 33.0 37.0 22.0 37.0 86-87 31.68980429627375 33.0 33.0 37.0 18.5 37.0 88-89 31.55241109148316 33.0 33.0 37.0 15.0 37.0 90-91 31.39497872283043 33.0 33.0 37.0 15.0 37.0 92-93 31.22530673221589 33.0 33.0 37.0 15.0 37.0 94-95 31.08121126717151 33.0 33.0 37.0 15.0 37.0 96-97 30.910758985245646 33.0 33.0 37.0 15.0 37.0 98-99 30.7592519567483 33.0 27.0 37.0 15.0 37.0 100-101 29.51133421049083 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.38202455406554847 2306 2 0.30067239015131975 2306 3 0.28666648521019056 2306 4 0.3678545499354229 2306 5 0.33047289811202774 2306 6 0.3642168814904849 2306 7 0.47846295399084937 2306 8 0.318141075560078 2306 9 0.44378350235151487 2306 10-11 0.457648213212444 2306 12-13 0.43639405983406476 2306 14-15 0.65835068266707 2306 16-17 0.6082452027966738 2306 18-19 0.618580346903542 2306 20-21 0.561375200470863 2306 22-23 0.47454957822760235 2306 24-25 0.5585796611688352 2306 26-27 0.5879107751024719 2306 28-29 0.5056815975678148 2306 30-31 0.42407938369168363 2306 32-33 0.45357470294725033 2306 34-35 0.34454298637706415 2306 36-37 0.4599418039408434 2306 38-39 0.37981708990166396 2306 40-41 0.3809439667253258 2306 42-43 0.4595311381276659 2306 44-45 0.43606898446045506 2306 46-47 0.2959019789459916 2306 48-49 0.4315493811633999 2306 50-51 0.4084731589161237 2306 52-53 0.3730279318912437 2306 54-55 0.39352143217026736 2306 56-57 0.4025662969400656 2306 58-59 0.5140116224269207 2306 60-61 0.3865697624828712 2306 62-63 0.5137990555112637 2306 64-65 0.45120793511338064 2306 66-67 0.5171239189664902 2306 68-69 0.5172286348457789 2306 70-71 0.5775447586029614 2306 72-73 0.5197580295969644 2306 74-75 0.4788892302612524 2306 76-77 0.5850638469839637 2306 78-79 0.47154281476792903 2306 80-81 0.5012757218104937 2306 82-83 0.5301520618144764 2306 84-85 0.47952299536551024 2306 86-87 0.5591850914962926 2306 88-89 0.6629781597078654 2306 90-91 0.4886747274058827 2306 92-93 0.4040082769844666 2306 94-95 0.4140376490019797 2306 96-97 0.2990147665325864 2306 98-99 0.19591813118632473 2306 100-101 0.29556355080343266 2307 1 0.271278149712753 2307 2 0.2769969854781813 2307 3 0.3233678491728611 2307 4 0.3917323394204857 2307 5 0.323339617461464 2307 6 0.3557924629307081 2307 7 0.5549347426576858 2307 8 0.3141019426837133 2307 9 0.4216313509454821 2307 10-11 0.46038272993005336 2307 12-13 0.4430531895359522 2307 14-15 0.6813858554105892 2307 16-17 0.5656974445642646 2307 18-19 0.6237845493192253 2307 20-21 0.5367244770959232 2307 22-23 0.5119215378722188 2307 24-25 0.5988901807405753 2307 26-27 0.5122973428779858 2307 28-29 0.5044429253279716 2307 30-31 0.5598749489797186 2307 32-33 0.48346421386589356 2307 34-35 0.42287453225932126 2307 36-37 0.27338380392733796 2307 38-39 0.38441847986800326 2307 40-41 0.5216971156303742 2307 42-43 0.4873552203764646 2307 44-45 0.46089851324878595 2307 46-47 0.3003043310482951 2307 48-49 0.3136305579768077 2307 50-51 0.358988685377156 2307 52-53 0.37967325166407306 2307 54-55 0.38941216415198454 2307 56-57 0.4527976524503359 2307 58-59 0.5779542775709174 2307 60-61 0.5636502614639554 2307 62-63 0.4144390884331699 2307 64-65 0.3782917207369607 2307 66-67 0.33891806153030757 2307 68-69 0.3857330231467344 2307 70-71 0.368025279004506 2307 72-73 0.33796131923062234 2307 74-75 0.32888183079270306 2307 76-77 0.35743662557545974 2307 78-79 0.4096827451707057 2307 80-81 0.41008342319182134 2307 82-83 0.3508977051135389 2307 84-85 0.4184576298956806 2307 86-87 0.4576808163282102 2307 88-89 0.4635585023774418 2307 90-91 0.49291781303371707 2307 92-93 0.5388893415334017 2307 94-95 0.48027941611326597 2307 96-97 0.3314189208004663 2307 98-99 0.4215937201526643 2307 100-101 0.222975940372244 2201 1 0.18945945347728355 2201 2 0.17372264528935943 2201 3 0.1494053753782758 2201 4 0.21461179927251095 2201 5 0.18509678768590732 2201 6 0.20768115102320905 2201 7 0.3751522407927439 2201 8 0.24129919336922967 2201 9 0.28969975013252025 2201 10-11 0.26594224796803445 2201 12-13 0.27620403746770705 2201 14-15 0.3776292898343314 2201 16-17 0.4870973125598823 2201 18-19 0.4636468598962722 2201 20-21 0.44597485053808583 2201 22-23 0.41782871484344497 2201 24-25 0.3996434233965189 2201 26-27 0.3782361120833784 2201 28-29 0.29831717911171296 2201 30-31 0.3535531080943173 2201 32-33 0.3131041738050655 2201 34-35 0.29740041871163214 2201 36-37 0.29388310756811364 2201 38-39 0.25724031521603763 2201 40-41 0.35606957439581066 2201 42-43 0.3654295167291224 2201 44-45 0.36969096514126676 2201 46-47 0.24296669608443722 2201 48-49 0.23634498755262 2201 50-51 0.2281226296068155 2201 52-53 0.2453168870972462 2201 54-55 0.49879599106370165 2201 56-57 0.557895854886489 2201 58-59 0.46131979654656874 2201 60-61 0.4091334750384945 2201 62-63 0.5329452018869674 2201 64-65 0.5107984921740538 2201 66-67 0.48002619311176176 2201 68-69 0.447103739760621 2201 70-71 0.4714414420869488 2201 72-73 0.43235511644603974 2201 74-75 0.4651128030944349 2201 76-77 0.5705415843793276 2201 78-79 0.45691726210810657 2201 80-81 0.46705250596630776 2201 82-83 0.35676853376971707 2201 84-85 0.4390448703422969 2201 86-87 0.455259193174566 2201 88-89 0.32211801815639163 2201 90-91 0.48265419380713936 2201 92-93 0.498167520307053 2201 94-95 0.41149897674109326 2201 96-97 0.3547610186123116 2201 98-99 0.11281153306402558 2201 100-101 0.1825174887774068 1101 1 0.1282966068676572 1101 2 -0.05419302322189168 1101 3 0.06332211434926194 1101 4 0.05604840096440711 1101 5 0.08836895842677706 1101 6 0.23543919865917928 1101 7 0.11076418948184141 1101 8 0.1564833546315043 1101 9 0.1712617165863648 1101 10-11 0.15248388534446633 1101 12-13 0.113798115680396 1101 14-15 0.3092005834183311 1101 16-17 0.2649968428486602 1101 18-19 0.2589239010764359 1101 20-21 0.2656843981138124 1101 22-23 0.26330198156022533 1101 24-25 0.14594792293626568 1101 26-27 0.13718360008645192 1101 28-29 0.12685362277610324 1101 30-31 0.23441249004360287 1101 32-33 0.2852199176449588 1101 34-35 0.19559789362622837 1101 36-37 0.07933463978432798 1101 38-39 0.15367155698490365 1101 40-41 0.18296688294693553 1101 42-43 0.08849035045765419 1101 44-45 -0.05626638725498623 1101 46-47 0.03275252281126484 1101 48-49 -0.1264801976303005 1101 50-51 0.08602080276003221 1101 52-53 0.13206529703758463 1101 54-55 0.35833942423035836 1101 56-57 0.2573660637277939 1101 58-59 0.17554044239060573 1101 60-61 0.2162187646547764 1101 62-63 0.29960759891367417 1101 64-65 0.251659505735752 1101 66-67 0.08921788927197838 1101 68-69 0.23651048345362113 1101 70-71 0.26668714715953 1101 72-73 0.28140979782669007 1101 74-75 0.3315517556297607 1101 76-77 0.3697464401798669 1101 78-79 0.4026671404996094 1101 80-81 0.2821702844929632 1101 82-83 0.30328753188853597 1101 84-85 0.1815667738494504 1101 86-87 0.11379654159599184 1101 88-89 0.20191070172168324 1101 90-91 0.020631040159575065 1101 92-93 0.2104669552405376 1101 94-95 0.1943362185740085 1101 96-97 0.24610089262743529 1101 98-99 0.24451017882572046 1101 100-101 -0.059183754348602235 2304 1 0.18385544321697722 2304 2 0.043749919087691325 2304 3 0.027165423639065267 2304 4 0.10267879142573122 2304 5 0.06686616740865503 2304 6 0.1121057247204007 2304 7 0.154709015904416 2304 8 0.14616291481766552 2304 9 0.15879563765047067 2304 10-11 0.17737807974555864 2304 12-13 0.12859402687399069 2304 14-15 0.2590740385159833 2304 16-17 0.2369193411262387 2304 18-19 0.29891490232782303 2304 20-21 0.2900105465088032 2304 22-23 0.3227928492364427 2304 24-25 0.15426403210233275 2304 26-27 0.18905084042334153 2304 28-29 0.15714425423517042 2304 30-31 0.1934948988961125 2304 32-33 0.1368108682783884 2304 34-35 0.08546516208421195 2304 36-37 0.06712586881653948 2304 38-39 0.008732650051655355 2304 40-41 0.10148954151472367 2304 42-43 0.025772587771527355 2304 44-45 0.10235061756777242 2304 46-47 0.03606799925282189 2304 48-49 0.0656290246809661 2304 50-51 -0.001415397518364614 2304 52-53 0.0045157687688544 2304 54-55 0.15263927940675615 2304 56-57 0.23457673286910818 2304 58-59 0.20065749642527209 2304 60-61 0.2960362587844969 2304 62-63 0.3487386572508697 2304 64-65 0.22362629472915785 2304 66-67 0.2472534905711754 2304 68-69 0.17502090934753056 2304 70-71 0.12091796506141606 2304 72-73 0.12039898526781911 2304 74-75 0.23404047204262923 2304 76-77 0.36464738364241356 2304 78-79 0.2227751254829471 2304 80-81 0.1555494552020349 2304 82-83 0.16485725109945548 2304 84-85 0.18104231553193628 2304 86-87 0.20046784824241115 2304 88-89 0.21831865436577047 2304 90-91 0.16116548146382925 2304 92-93 0.20986516392714094 2304 94-95 0.23327338199971948 2304 96-97 0.24444385743389319 2304 98-99 0.10609958279922793 2304 100-101 0.1341016325272868 2202 1 0.19233731825935863 2202 2 0.18765357955186346 2202 3 0.1575714811151876 2202 4 0.12038172887389464 2202 5 0.15201616975610932 2202 6 0.2698567589045524 2202 7 0.2213008248529107 2202 8 0.24025384999139732 2202 9 0.22244791181229573 2202 10-11 0.22114923944028675 2202 12-13 0.21204160299672736 2202 14-15 0.30726115151201583 2202 16-17 0.346197655340859 2202 18-19 0.24702859216125717 2202 20-21 0.29832836055910406 2202 22-23 0.27196576274057094 2202 24-25 0.2233193642149942 2202 26-27 0.30906380358917573 2202 28-29 0.2656706005003642 2202 30-31 0.2876366955856824 2202 32-33 0.12742991813311022 2202 34-35 0.1533832828685746 2202 36-37 0.08811367770243095 2202 38-39 0.12405735518527194 2202 40-41 0.25735100517873377 2202 42-43 0.18881700161212223 2202 44-45 0.26261212400762446 2202 46-47 0.2575705527617487 2202 48-49 0.21826612906966147 2202 50-51 0.20429035165872023 2202 52-53 0.2258392636592248 2202 54-55 0.359540535034121 2202 56-57 0.4267222802694377 2202 58-59 0.2765660917606212 2202 60-61 0.35773858067790343 2202 62-63 0.5363707015708101 2202 64-65 0.4567874813935262 2202 66-67 0.38533455078599843 2202 68-69 0.4605458523565389 2202 70-71 0.42368757021956327 2202 72-73 0.49934049191632823 2202 74-75 0.3966117433718708 2202 76-77 0.41566865679744325 2202 78-79 0.3814264084972905 2202 80-81 0.3813126387677741 2202 82-83 0.37031325719240016 2202 84-85 0.3568756619066811 2202 86-87 0.4059245064060697 2202 88-89 0.4085180315078283 2202 90-91 0.4642879872933392 2202 92-93 0.46680580462574284 2202 94-95 0.5072883639764854 2202 96-97 0.49113929346054164 2202 98-99 0.43814298984263544 2202 100-101 0.608674395794317 1102 1 -0.12956016187856534 1102 2 -0.19587322316283817 1102 3 -0.07599275045859955 1102 4 -0.12850486037893916 1102 5 -0.16249954384399956 1102 6 0.0631009476503337 1102 7 -0.11803514545439953 1102 8 -0.0512760448426377 1102 9 -0.1860690120310622 1102 10-11 -0.0174334913797054 1102 12-13 -0.040368287351057575 1102 14-15 -0.051521768278071534 1102 16-17 -0.07958145505210723 1102 18-19 -0.0218255435320458 1102 20-21 -0.057488126527289296 1102 22-23 -0.051470685795408144 1102 24-25 -0.16605178530743814 1102 26-27 -0.018619010956697934 1102 28-29 -0.1302562234718394 1102 30-31 -0.12698678900196825 1102 32-33 -0.15835898310164964 1102 34-35 -0.2315995937544315 1102 36-37 -0.13279451334906867 1102 38-39 -0.211251426381466 1102 40-41 -0.19277797440884115 1102 42-43 -0.2849028368569222 1102 44-45 -0.344378416402499 1102 46-47 -0.24053762707413995 1102 48-49 -0.18717750398145228 1102 50-51 -0.1451388582402302 1102 52-53 -0.08914797857721624 1102 54-55 -0.014916453077326253 1102 56-57 0.10517121269926122 1102 58-59 0.10400144546986212 1102 60-61 0.12740568553609677 1102 62-63 0.04801546192178563 1102 64-65 0.11775447944253159 1102 66-67 0.08765954152659106 1102 68-69 0.07824543164051789 1102 70-71 0.046103740008341276 1102 72-73 0.05041490883468924 1102 74-75 -0.06241653432679328 1102 76-77 -0.022560684298142064 1102 78-79 -0.04549716751068189 1102 80-81 0.012370049681869943 1102 82-83 0.11143368422047928 1102 84-85 0.16774613718798648 1102 86-87 0.1239219352912464 1102 88-89 0.0771562339836045 1102 90-91 0.15093458974408236 1102 92-93 0.07033490915007334 1102 94-95 0.1847463119360242 1102 96-97 0.13137989777359138 1102 98-99 0.05707490127056758 1102 100-101 -0.00869295345997756 2305 1 0.16441640492178067 2305 2 0.08090810301471763 2305 3 0.12636555928426318 2305 4 0.11949732173948746 2305 5 0.17332061814389021 2305 6 0.22950959355738831 2305 7 0.14834671544046785 2305 8 0.2630522165397693 2305 9 0.12087616961555625 2305 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-1.7328996099602918 1213 62-63 -1.297835182635069 1213 64-65 -1.2144876398815967 1213 66-67 -1.1849246944732528 1213 68-69 -1.248477331666642 1213 70-71 -1.3085731870411976 1213 72-73 -1.4374294948536601 1213 74-75 -1.6056830339229116 1213 76-77 -1.3403651742363856 1213 78-79 -1.2679168158459362 1213 80-81 -1.363022080566946 1213 82-83 -1.491055104175956 1213 84-85 -1.4936141635831426 1213 86-87 -1.5442629187813495 1213 88-89 -1.6051246321348387 1213 90-91 -1.3884162904109338 1213 92-93 -1.4542866164666783 1213 94-95 -1.564868758180637 1213 96-97 -1.5345820572608133 1213 98-99 -1.5494444227447772 1213 100-101 -1.5100791479592246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1963.0 3 361.0 4 390.0 5 582.0 6 1070.0 7 3699.0 8 15143.0 9 33932.0 10 44237.0 11 35483.0 12 24513.0 13 18980.0 14 17116.0 15 16923.0 16 17249.0 17 17460.0 18 18278.0 19 19547.0 20 21495.0 21 23809.0 22 26008.0 23 28862.0 24 32606.0 25 37077.0 26 42948.0 27 50989.0 28 61385.0 29 74810.0 30 91278.0 31 120059.0 32 161812.0 33 229413.0 34 344333.0 35 574896.0 36 1077703.0 37 1571433.0 38 333099.0 39 69.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.83027560028415 24.066483572577262 26.402081490700585 13.701159336437996 2 31.521600675645946 22.265185628936667 34.34882229014022 11.864391405277171 3 38.58778617117173 18.08645410147809 27.76713168195794 15.558628045392242 4 29.860170515103174 16.84847663964696 36.57044996569477 16.720902879555087 5 31.01454594361269 15.976147762998929 37.24844459340997 15.760861699978406 6 24.836289303977257 16.83280535050046 43.15895942938492 15.171945916137366 7 34.0939817391145 17.415121216826556 31.918299559921724 16.57259748413722 8 29.614981176330367 28.23313231995436 25.991378969522454 16.160507534192813 9 30.953204721724653 26.728044921492298 24.417537217292832 17.901213139490213 10-11 25.561018687768495 27.27480969018256 28.851122577261545 18.313049044787398 12-13 26.732976559029314 24.1273751008624 28.42716582404891 20.712482516059378 14-15 24.802854623015456 25.061073650376954 29.844808223819907 20.291263502787682 16-17 23.7297019548046 26.72541760875579 31.17794398251048 18.36693645392913 18-19 22.812787860852698 27.211053393900247 31.098108426651716 18.878050318595342 20-21 22.68828807703611 28.135377046676606 30.10403176104243 19.072303115244853 22-23 23.65894321810817 27.412805914620105 30.09873288030826 18.829517986963467 24-25 22.894071679947917 28.120830911369126 30.197267472808605 18.787829935874356 26-27 22.188971165233877 28.568675005173805 30.16157926782963 19.080774561762684 28-29 21.95520949149749 28.381760370518407 29.747308143221545 19.915721994762556 30-31 22.310346998913698 28.005258448981614 29.517313632563752 20.16708091954094 32-33 21.053049525352456 28.061371616207136 30.779303019456854 20.106275838983553 34-35 20.552819045517236 28.159892878338106 32.155978044619985 19.131310031524674 36-37 20.743924764163474 29.078969612952775 30.732467432098886 19.444638190784865 38-39 20.807686371040333 28.90270129263106 29.312447004600724 20.977165331727882 40-41 21.525532546800594 27.985196769940767 29.951480298844167 20.53779038441447 42-43 21.762053900858437 28.259198123788497 31.086162166677756 18.892585808675314 44-45 20.83096636073687 29.377117832422012 31.223105183268913 18.568810623572205 46-47 21.786972673412038 29.025381662601852 30.521437195440832 18.66620846854528 48-49 21.370236658312823 29.473346300850324 29.88795821731085 19.268458823526 50-51 20.500836742013732 29.569457321323434 29.860697681388366 20.069008255274472 52-53 21.81079546141112 29.000567927788584 29.367106105827904 19.82153050497239 54-55 22.69366789815111 28.818949171717424 29.077929122975416 19.409453807156048 56-57 22.907566314904273 28.56116050176284 28.374828730296432 20.156444453036457 58-59 22.064187437043234 28.29468183383067 29.18101064468308 20.460120084443012 60-61 20.570150587438654 28.39067163342653 29.421530038255593 21.617647740879224 62-63 21.793517943373903 28.370110520963344 29.076458257879157 20.7599132777836 64-65 20.950597451250893 28.738848884434265 29.626715210587406 20.683838453727436 66-67 21.18904199561945 29.043443367674993 28.714636357635566 21.052878279069997 68-69 21.594810650431285 28.538359360157767 30.000543077758877 19.86628691165207 70-71 20.963832185918665 30.651643670062466 29.071376754601413 19.313147389417455 72-73 20.441452641888556 31.769872938129566 28.28475507769755 19.50391934228433 74-75 21.619233628705906 31.13859097476813 27.622051930372393 19.62012346615357 76-77 22.091755910351278 29.90106698266083 28.290217428896963 19.71695967809093 78-79 21.936633349986682 29.820291661928465 29.02866021575667 19.214414772328183 80-81 20.73542782880084 30.681627348577443 28.81661167126438 19.766333151357333 82-83 19.81842997950446 30.27805543093112 29.122308474575615 20.781206114988805 84-85 19.629740309269124 29.38454868275801 29.92667523997168 21.059035768001184 86-87 19.887495878244206 29.387732263113563 30.597721273053324 20.12705058558891 88-89 20.10848863340799 29.990219553103458 29.989881263112967 19.91141055037558 90-91 20.440697288293237 29.63239435585222 29.439783425293054 20.48712493056149 92-93 19.789597835083804 30.551351729503747 29.23770305993659 20.421347375475865 94-95 19.60923086971586 30.970345883212747 29.40092334204913 20.019499905022265 96-97 20.397027356975585 31.28910911615487 28.565777122102315 19.748086404767236 98-99 20.78555771706734 30.4040865327938 28.226639342202965 20.583716407935896 100-101 20.51115221698231 30.315414656603096 28.221746740976773 20.951686385437828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3179.0 1 2267.5 2 1127.5 3 1081.5 4 1177.5 5 1059.5 6 1309.0 7 1817.5 8 2265.0 9 2555.0 10 2723.0 11 2826.0 12 2769.0 13 2612.0 14 2349.5 15 2133.5 16 1954.0 17 1770.0 18 1822.0 19 1971.5 20 2205.0 21 2569.5 22 3070.5 23 3769.0 24 4586.0 25 5864.0 26 7663.0 27 9782.5 28 12136.0 29 14960.5 30 18528.0 31 23571.0 32 34572.0 33 54686.5 34 82990.0 35 123080.5 36 173867.0 37 257652.5 38 363614.5 39 423492.5 40 415219.5 41 357040.0 42 310712.0 43 305706.5 44 301694.5 45 293960.5 46 294273.0 47 265641.5 48 209874.5 49 168225.0 50 139538.0 51 116967.5 52 95517.5 53 70464.5 54 48619.0 55 35407.0 56 26162.5 57 19195.0 58 14218.0 59 10458.0 60 7743.0 61 5858.0 62 4382.0 63 3181.5 64 2351.0 65 1701.0 66 1239.0 67 908.5 68 655.0 69 478.5 70 362.5 71 287.5 72 226.0 73 179.5 74 130.5 75 101.0 76 83.0 77 68.5 78 66.5 79 51.5 80 34.5 81 35.0 82 29.5 83 23.5 84 20.0 85 15.0 86 10.5 87 7.0 88 11.5 89 9.5 90 6.5 91 7.5 92 4.0 93 2.0 94 3.0 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17990718569218705 2 0.18489658081953222 3 0.15600047004340195 4 0.15653986411122306 5 0.16572882733803249 6 0.16166410775552348 7 0.1291656151693023 8 0.11140413907890756 9 0.11146193130045984 10-11 0.09111906931406412 12-13 0.13421280251819973 14-15 0.11321496202087841 16-17 0.10603909451147271 18-19 0.10923693077069781 20-21 0.10182989437508307 22-23 0.10309169121230743 24-25 0.46018982818372534 26-27 0.3063565664485331 28-29 0.39954652370155325 30-31 0.2661909724697121 32-33 0.2952700919474245 34-35 0.31464011820435717 36-37 0.2944513688087675 38-39 0.3429294106541887 40-41 0.33797854367454505 42-43 0.38023428966617284 44-45 0.33486739574764834 46-47 0.35783017177774656 48-49 0.3533320105335956 50-51 0.3691959753496911 52-53 0.36019002082446383 54-55 0.3360328722156189 56-57 0.35152118759162476 58-59 0.34322800379887536 60-61 0.31481349486901394 62-63 0.32340527180645 64-65 0.3248693414191072 66-67 0.3363218333233802 68-69 0.3235786484711068 70-71 0.3118468274959979 72-73 0.2978322137695747 74-75 0.30644325478086154 76-77 0.2964163043415443 78-79 0.30580754034378665 80-81 0.31467864635205867 82-83 0.3407622023459789 84-85 0.33879726681320205 86-87 0.3321896894823936 88-89 0.3454722684024881 90-91 0.334578434639887 92-93 0.3176838418727762 94-95 0.3104020219571914 96-97 0.2956361093505888 98-99 0.3207083014673445 100-101 0.32434921142513695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5191010.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.719153955647066 #Duplication Level Percentage of deduplicated Percentage of total 1 86.44063474513504 31.74026975230469 2 5.926513019680789 4.352330879796114 3 2.3785499361269666 2.6201502388752176 4 1.1043330464732917 1.6220070060704619 5 0.6546048478387649 1.2018268093952265 6 0.4550107217408806 1.0024565245844088 7 0.291752350187696 0.7499029624424707 8 0.3346968837352442 0.9831829121879792 9 0.2657656308508946 0.8782820203800298 >10 1.6462987014401214 12.049003838319376 >50 0.23224378883149174 5.994276067730224 >100 0.2179898061811637 17.172621787686623 >500 0.03643178134211537 9.149998270366225 >1k 0.014480748719026264 8.510584349412886 >5k 5.872250341645187E-4 1.436602270184588 >10k+ 1.0676668231498914E-4 0.536504310263394 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 14137 0.27233621202810243 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13233 0.25492148926702124 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 9492 0.18285458899135235 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 9227 0.17774960942090268 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6910 0.1331147503087068 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6616 0.12745111259658523 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 6540 0.125987042983928 Illumina Single End PCR Primer 1 (96% over 32bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6252 0.12043898971491097 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5937 0.11437080645192362 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 5707 0.1099400694662503 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG 5565 0.1072045709794433 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5561 0.10712751468404029 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 5481 0.10558638877598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016181822034632952 0.0 0.0 2 0.0017145025727170627 0.0 0.0 3 0.001945671458926105 0.0 0.0 4 0.0020419918281798727 0.0 3.8528147701507025E-5 5 0.0021961044189859006 0.0 3.8528147701507025E-5 6 0.002427273305194943 0.0 5.7792221552260545E-5 7 0.00285108292991152 0.0 5.7792221552260545E-5 8 0.0033134207023296043 0.0 5.7792221552260545E-5 9 0.0036601740316431677 0.0 5.7792221552260545E-5 10-11 0.0060874473368381105 0.0 5.7792221552260545E-5 12-13 0.008967426377525761 0.0 5.7792221552260545E-5 14-15 0.012261583006004611 0.0 5.7792221552260545E-5 16-17 0.015969917222274664 0.0 5.7792221552260545E-5 18-19 0.02049697457720174 0.0 5.7792221552260545E-5 20-21 0.024523166012009222 0.0 5.7792221552260545E-5 22-23 0.02922360003159308 0.0 5.7792221552260545E-5 24-25 0.03529178329458044 0.0 5.7792221552260545E-5 26-27 0.04215942562237407 0.0 5.7792221552260545E-5 28-29 0.049720574608794824 0.0 5.7792221552260545E-5 30-31 0.07768237780316355 0.0 5.7792221552260545E-5 32-33 0.0877767525009584 0.0 5.7792221552260545E-5 34-35 0.09703313998624545 0.0 5.7792221552260545E-5 36-37 0.10729125931177169 0.0 5.7792221552260545E-5 38-39 0.11828141344362658 0.0 5.7792221552260545E-5 40-41 0.13004213052951158 0.0 5.7792221552260545E-5 42-43 0.14311280463724785 0.0 5.7792221552260545E-5 44-45 0.15665544855432756 0.0 7.705629540301405E-5 46-47 0.1711805602377957 0.0 7.705629540301405E-5 48-49 0.18692894061078674 0.0 7.705629540301405E-5 50-51 0.20352494023321088 0.0 7.705629540301405E-5 52-53 0.22040990096339635 0.0 7.705629540301405E-5 54-55 0.23777646353985063 0.0 7.705629540301405E-5 56-57 0.25653967147048456 0.0 7.705629540301405E-5 58-59 0.2768632693830295 0.0 7.705629540301405E-5 60-61 0.2991999630129782 0.0 7.705629540301405E-5 62-63 0.3220375225630465 0.0 7.705629540301405E-5 64-65 0.3461176148764884 0.0 7.705629540301405E-5 66-67 0.3721433786488564 0.0 7.705629540301405E-5 68-69 0.4006445759110462 0.0 7.705629540301405E-5 70-71 0.42956958279795265 0.0 7.705629540301405E-5 72-73 0.4603150446637552 0.0 7.705629540301405E-5 74-75 0.4926883207699465 0.0 7.705629540301405E-5 76-77 0.5274118138859297 0.0 8.668833232839082E-5 78-79 0.5644951560486302 0.0 1.348485169552746E-4 80-81 0.6046318539166752 0.0 1.348485169552746E-4 82-83 0.6494786178412293 0.0 1.4448055388065136E-4 84-85 0.6962903172985604 0.0 1.541125908060281E-4 86-87 0.7470896800429974 0.0 1.6374462773140487E-4 88-89 0.8005474849788384 0.0 1.7337666465678164E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1585 0.0 77.13416 9 AGAGCGG 1655 0.0 76.73494 8 CGGGAGA 1980 0.0 76.60523 4 GAGAGCG 1885 0.0 71.14356 7 CGGAAAG 685 0.0 69.88762 5 ATTCGGA 310 0.0 62.695087 2 AAGCGGC 680 0.0 61.323948 9 GAACCGG 315 0.0 60.197376 1 TCGGAGA 485 0.0 59.615852 4 TCGGGAG 2410 0.0 58.216797 3 AAGCGTC 300 0.0 56.86402 9 TCGGGAA 720 0.0 56.616055 3 CGGAGAG 990 0.0 50.75048 5 TTCGGAG 395 0.0 49.19947 3 AAAGCGG 925 0.0 49.179695 8 GGATCGG 785 0.0 47.70738 1 GGTCCGG 250 0.0 47.405437 1 CGTTGCG 50 2.8618892E-5 47.40543 1 AACCGGA 370 0.0 46.122425 2 ACCGGAA 360 0.0 44.766182 3 >>END_MODULE