FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34298-1.342000000357ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34298-1.342000000357ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191010
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC141370.27233621202810243No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG132330.25492148926702124No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA94920.18285458899135235No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA92270.17774960942090268No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA69100.1331147503087068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66160.12745111259658523No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT65400.125987042983928Illumina Single End PCR Primer 1 (96% over 32bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62520.12043898971491097No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT59370.11437080645192362No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC57070.1099400694662503No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG55650.1072045709794433No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT55610.10712751468404029No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT54810.10558638877598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC15850.077.134169
AGAGCGG16550.076.734948
CGGGAGA19800.076.605234
GAGAGCG18850.071.143567
CGGAAAG6850.069.887625
ATTCGGA3100.062.6950872
AAGCGGC6800.061.3239489
GAACCGG3150.060.1973761
TCGGAGA4850.059.6158524
TCGGGAG24100.058.2167973
AAGCGTC3000.056.864029
TCGGGAA7200.056.6160553
CGGAGAG9900.050.750485
TTCGGAG3950.049.199473
AAAGCGG9250.049.1796958
GGATCGG7850.047.707381
GGTCCGG2500.047.4054371
CGTTGCG502.8618892E-547.405431
AACCGGA3700.046.1224252
ACCGGAA3600.044.7661823
Produced by FastQC (version 0.11.2)