##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34274-2.3420000003583a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4492544 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.285233043905635 33.0 33.0 33.0 33.0 33.0 2 32.27455423920166 33.0 33.0 33.0 33.0 33.0 3 32.24984017963987 33.0 33.0 33.0 33.0 33.0 4 36.058878221337395 37.0 37.0 37.0 37.0 37.0 5 36.074484523690806 37.0 37.0 37.0 37.0 37.0 6 36.03649090582084 37.0 37.0 37.0 33.0 37.0 7 35.97556351145364 37.0 37.0 37.0 33.0 37.0 8 35.93988929212491 37.0 37.0 37.0 33.0 37.0 9 35.9061965781526 37.0 37.0 37.0 33.0 37.0 10-11 35.832948770229066 37.0 37.0 37.0 33.0 37.0 12-13 35.81802682399994 37.0 37.0 37.0 33.0 37.0 14-15 37.65817340909739 40.0 37.0 40.0 33.0 40.0 16-17 37.64163088886831 40.0 37.0 40.0 33.0 40.0 18-19 37.63340793100747 40.0 37.0 40.0 33.0 40.0 20-21 37.63729025247165 40.0 37.0 40.0 33.0 40.0 22-23 37.5948930049433 40.0 37.0 40.0 33.0 40.0 24-25 37.3890596285757 40.0 37.0 40.0 33.0 40.0 26-27 37.33022948690096 40.0 37.0 40.0 33.0 40.0 28-29 37.26363447970682 40.0 37.0 40.0 33.0 40.0 30-31 37.290984462255686 40.0 37.0 40.0 33.0 40.0 32-33 37.23941457223346 40.0 37.0 40.0 33.0 40.0 34-35 37.19885358941393 40.0 37.0 40.0 33.0 40.0 36-37 37.112614255976126 40.0 37.0 40.0 33.0 40.0 38-39 37.0562673398413 40.0 37.0 40.0 33.0 40.0 40-41 36.89364978951792 40.0 37.0 40.0 33.0 40.0 42-43 36.82996070823124 40.0 37.0 40.0 33.0 40.0 44-45 36.70996299646704 40.0 37.0 40.0 33.0 40.0 46-47 36.65071449494985 40.0 37.0 40.0 33.0 40.0 48-49 36.538962667922675 40.0 37.0 40.0 33.0 40.0 50-51 35.33790420305288 37.0 35.0 38.5 30.0 38.5 52-53 35.75959634452106 37.0 37.0 38.5 33.0 40.0 54-55 36.55504865394752 37.0 37.0 40.0 33.0 40.0 56-57 36.5574457367585 38.5 37.0 40.0 33.0 40.0 58-59 36.39143244896432 37.0 37.0 40.0 33.0 40.0 60-61 36.171264766688985 37.0 37.0 40.0 33.0 40.0 62-63 35.89502985390905 37.0 37.0 40.0 33.0 40.0 64-65 35.69437127382614 37.0 37.0 40.0 33.0 40.0 66-67 35.45086492196849 37.0 37.0 40.0 27.0 40.0 68-69 35.22371288962334 37.0 35.0 37.0 27.0 40.0 70-71 34.894853784403665 37.0 33.0 37.0 27.0 40.0 72-73 34.62891426327711 37.0 33.0 37.0 27.0 40.0 74-75 34.384592449178015 37.0 33.0 37.0 27.0 38.5 76-77 34.13964526557781 37.0 33.0 37.0 27.0 37.0 78-79 33.905926352641174 37.0 33.0 37.0 27.0 37.0 80-81 33.66286351341245 37.0 33.0 37.0 27.0 37.0 82-83 33.446501247400136 37.0 33.0 37.0 27.0 37.0 84-85 33.27387989967377 37.0 33.0 37.0 27.0 37.0 86-87 33.110298641482416 37.0 33.0 37.0 27.0 37.0 88-89 32.94921184967804 37.0 33.0 37.0 27.0 37.0 90-91 32.80054953273691 37.0 33.0 37.0 27.0 37.0 92-93 32.67796442283036 37.0 33.0 37.0 27.0 37.0 94-95 32.54216130103567 33.0 33.0 37.0 24.5 37.0 96-97 32.37744605283777 33.0 33.0 37.0 22.0 37.0 98-99 32.23097915123369 33.0 33.0 37.0 22.0 37.0 100-101 30.982459158997663 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.33251506426415744 2306 2 0.2646799468850105 2306 3 0.2631789810745033 2306 4 0.35776209538626347 2306 5 0.3450053169929106 2306 6 0.34164324845983174 2306 7 0.4582015282593517 2306 8 0.3555490583526293 2306 9 0.4206758122158334 2306 10-11 0.47859782943615414 2306 12-13 0.37467141679683635 2306 14-15 0.6463393665766901 2306 16-17 0.6257116350372272 2306 18-19 0.6196640775122191 2306 20-21 0.5625489212486627 2306 22-23 0.560019220134734 2306 24-25 0.5578904294735949 2306 26-27 0.6120851341202567 2306 28-29 0.48422889819539705 2306 30-31 0.447031777266254 2306 32-33 0.3761743066797507 2306 34-35 0.31611386517315765 2306 36-37 0.32899542542758553 2306 38-39 0.36601982518114085 2306 40-41 0.2954250320344016 2306 42-43 0.22295243539684861 2306 44-45 0.3091242846699984 2306 46-47 0.23480686608049695 2306 48-49 0.3853816416881486 2306 50-51 0.4035572895308164 2306 52-53 0.3363544942045138 2306 54-55 0.42068758950349405 2306 56-57 0.38464265356873284 2306 58-59 0.43271871133008943 2306 60-61 0.4119857975862473 2306 62-63 0.5425478330732645 2306 64-65 0.4282899872650461 2306 66-67 0.46945284594415426 2306 68-69 0.41556874008325195 2306 70-71 0.39345725422765554 2306 72-73 0.40312812931221487 2306 74-75 0.3336872145883092 2306 76-77 0.36265467265950235 2306 78-79 0.35856548643998565 2306 80-81 0.41880092632285226 2306 82-83 0.327172344867769 2306 84-85 0.301243105894045 2306 86-87 0.41028948901855244 2306 88-89 0.4429769195178892 2306 90-91 0.526764986926409 2306 92-93 0.4233201018099564 2306 94-95 0.48900288245749124 2306 96-97 0.4256821601111582 2306 98-99 0.32370453576907465 2306 100-101 0.29305856383638584 2307 1 0.2187660886366345 2307 2 0.18783446398823855 2307 3 0.20586391824632244 2307 4 0.2821947068531685 2307 5 0.21344174519578019 2307 6 0.310660723495495 2307 7 0.266820769009648 2307 8 0.24650982488675766 2307 9 0.32195381173020365 2307 10-11 0.3718612999342241 2307 12-13 0.3602004956962759 2307 14-15 0.5130784664947328 2307 16-17 0.4578833805679281 2307 18-19 0.5374050706223628 2307 20-21 0.4895031014471556 2307 22-23 0.35606310311345624 2307 24-25 0.2205859138879589 2307 26-27 0.33586617829727317 2307 28-29 0.2050501698589784 2307 30-31 0.1865701362215546 2307 32-33 0.21533786861255777 2307 34-35 0.1945267802337156 2307 36-37 0.06698774073702651 2307 38-39 -0.026321472964369264 2307 40-41 0.170440207922951 2307 42-43 0.11884821118827205 2307 44-45 0.08061310573109637 2307 46-47 -0.044510816485448856 2307 48-49 0.012089403396345233 2307 50-51 -0.09016700630795782 2307 52-53 0.022064450725586937 2307 54-55 0.042313562657348314 2307 56-57 0.08691187572856762 2307 58-59 0.05953151864183326 2307 60-61 0.11585346763207838 2307 62-63 -0.03995375091009379 2307 64-65 -0.04770294266787545 2307 66-67 -0.006973308182168125 2307 68-69 -0.0017537304755279592 2307 70-71 -0.023793367983770963 2307 72-73 0.00782516166720626 2307 74-75 0.02141295593047232 2307 76-77 -0.03615013126801614 2307 78-79 -0.0057528221313560834 2307 80-81 0.006339719687950662 2307 82-83 -0.03234658895017617 2307 84-85 0.004262111797459056 2307 86-87 0.08781086805154104 2307 88-89 0.04803414210265089 2307 90-91 0.08936102184613048 2307 92-93 -0.043578439787154366 2307 94-95 0.034902120248489155 2307 96-97 0.0352414048830596 2307 98-99 -0.014835154870432632 2307 100-101 -0.0738375116788319 2201 1 0.21639488190827194 2201 2 0.18829544041788182 2201 3 0.24037123784346193 2201 4 0.3121956625862481 2201 5 0.18298681624959556 2201 6 0.3040389762836 2201 7 0.3629481550467162 2201 8 0.3320722408481558 2201 9 0.2805944626833039 2201 10-11 0.3594551018404246 2201 12-13 0.3933102616405435 2201 14-15 0.5365404777526095 2201 16-17 0.5367461900277064 2201 18-19 0.46148855555473745 2201 20-21 0.5418719188428156 2201 22-23 0.49140088060561027 2201 24-25 0.39932159436990844 2201 26-27 0.48665469928385363 2201 28-29 0.43641152100190084 2201 30-31 0.3200079822628368 2201 32-33 0.25451592168540316 2201 34-35 0.25696354530793286 2201 36-37 0.28115042792888545 2201 38-39 0.36350969184356074 2201 40-41 0.32443187656699735 2201 42-43 0.2683977807788196 2201 44-45 0.29726523042459974 2201 46-47 0.3185600280530352 2201 48-49 0.2818817291892728 2201 50-51 0.2078524016648302 2201 52-53 0.20985261248835485 2201 54-55 0.3775977037499203 2201 56-57 0.4060280615626297 2201 58-59 0.45860136789965367 2201 60-61 0.38656362674515776 2201 62-63 0.4334778993443251 2201 64-65 0.3356150247844667 2201 66-67 0.25865622331088645 2201 68-69 0.3680377302141764 2201 70-71 0.35041156096241366 2201 72-73 0.3026527507151613 2201 74-75 0.3415077382869782 2201 76-77 0.44435332584922094 2201 78-79 0.3483353673147107 2201 80-81 0.4529723260050176 2201 82-83 0.28113283148009316 2201 84-85 0.2711067013595425 2201 86-87 0.32773571117949274 2201 88-89 0.3906819594060309 2201 90-91 0.41961808932747147 2201 92-93 0.3397883442039884 2201 94-95 0.28745685244549435 2201 96-97 0.36311531897253957 2201 98-99 0.3261269470173005 2201 100-101 0.20278789368381567 1101 1 0.11793947249312708 1101 2 0.10125399524789458 1101 3 0.10265106420302317 1101 4 0.1959180956548252 1101 5 0.14673912301916658 1101 6 0.2074322226239076 1101 7 0.2582499773496778 1101 8 0.2581056700163842 1101 9 0.2512807585819843 1101 10-11 0.2989392566825302 1101 12-13 0.1932259470976092 1101 14-15 0.35116566684830985 1101 16-17 0.28251759249320685 1101 18-19 0.22393458092686558 1101 20-21 0.2713030584859908 1101 22-23 0.30624234622421653 1101 24-25 0.10862551697621825 1101 26-27 0.1907701454057147 1101 28-29 0.1664060776377312 1101 30-31 0.16545532095457105 1101 32-33 0.14332710823078543 1101 34-35 0.1307305081195338 1101 36-37 0.019381492977807113 1101 38-39 0.0694985444673577 1101 40-41 0.11404981144391257 1101 42-43 -0.05950109865138842 1101 44-45 -0.04564511175131969 1101 46-47 -0.04420995308959874 1101 48-49 -0.09802350875270349 1101 50-51 -0.02089849746228367 1101 52-53 -0.07833013910342856 1101 54-55 0.08389481567080992 1101 56-57 0.07080134112474212 1101 58-59 0.010298387081753901 1101 60-61 0.09577124644697221 1101 62-63 0.17215339779735217 1101 64-65 0.13678291800813014 1101 66-67 0.10675711203368365 1101 68-69 0.13061524955216441 1101 70-71 0.16346053251939452 1101 72-73 0.1537198588991444 1101 74-75 0.24668555845003226 1101 76-77 0.008631284472457423 1101 78-79 0.05447056435225761 1101 80-81 0.1283206545106026 1101 82-83 0.21827358564524246 1101 84-85 0.05547369786815182 1101 86-87 0.04149026801692912 1101 88-89 0.13127478568567597 1101 90-91 -4.317793765693523E-4 1101 92-93 0.16553156861963458 1101 94-95 0.150652816435759 1101 96-97 0.22733587386448306 1101 98-99 0.14015604813499039 1101 100-101 -0.04140801126467508 2304 1 0.18021826723197165 2304 2 0.1456988547965139 2304 3 0.1072556470061059 2304 4 0.22189931581203837 2304 5 0.23025855108686955 2304 6 0.28744765920001214 2304 7 0.24739978129244378 2304 8 0.2598843737414285 2304 9 0.2667658223830571 2304 10-11 0.28343940810723467 2304 12-13 0.2251585555530724 2304 14-15 0.2917226474476706 2304 16-17 0.2876840762049184 2304 18-19 0.3003060078403976 2304 20-21 0.366272300591433 2304 22-23 0.3330014362770015 2304 24-25 0.1538303218688668 2304 26-27 0.2501420627604105 2304 28-29 0.12398112825174223 2304 30-31 0.16111630767132823 2304 32-33 0.09669092257535539 2304 34-35 0.07216038655848678 2304 36-37 0.09262721195104717 2304 38-39 0.10208994974425423 2304 40-41 0.163694857630432 2304 42-43 0.2087302767062127 2304 44-45 0.12735844692793563 2304 46-47 0.08839027535901067 2304 48-49 0.15592299262124953 2304 50-51 0.004677293980194008 2304 52-53 0.041914071981814516 2304 54-55 0.21443928485369668 2304 56-57 0.26275599956068874 2304 58-59 0.17400641640647763 2304 60-61 0.22317593830763371 2304 62-63 0.2576772358611592 2304 64-65 0.1573221886736249 2304 66-67 0.1708590840523101 2304 68-69 0.19450653853805733 2304 70-71 0.16363914213061292 2304 72-73 0.20449987497162425 2304 74-75 0.08652559673147664 2304 76-77 0.21935684329320537 2304 78-79 0.16416428901047908 2304 80-81 0.06946413756494252 2304 82-83 0.18566334170441223 2304 84-85 0.09263506890955142 2304 86-87 0.046202080177167204 2304 88-89 0.17731588417849764 2304 90-91 0.019528500453105835 2304 92-93 0.14717726501827144 2304 94-95 0.1788787389142641 2304 96-97 0.2515178823840074 2304 98-99 0.1460736937630287 2304 100-101 0.1336187563305984 2202 1 0.16770482748044913 2202 2 0.1130719408705545 2202 3 0.12512741883224976 2202 4 0.12602003022298902 2202 5 0.13122264235612846 2202 6 0.18658248021697688 2202 7 0.2167700385465139 2202 8 0.1322421034152228 2202 9 0.24042980127796199 2202 10-11 0.26718372734610085 2202 12-13 0.22483750126951207 2202 14-15 0.3822595626198506 2202 16-17 0.3540402225679671 2202 18-19 0.2441281499805683 2202 20-21 0.2694896994411238 2202 22-23 0.25259296067245884 2202 24-25 0.1930148704128385 2202 26-27 0.21463794397426028 2202 28-29 0.19770488301925582 2202 30-31 0.07230653670861642 2202 32-33 -0.02589971518035128 2202 34-35 0.03986416818586491 2202 36-37 8.342535774019666E-4 2202 38-39 -0.09148885745928226 2202 40-41 0.01521019742627061 2202 42-43 0.014101606640821274 2202 44-45 0.1650392787624142 2202 46-47 0.07411294744764518 2202 48-49 0.054721476156636584 2202 50-51 -0.013382554023401383 2202 52-53 -0.010696051851368793 2202 54-55 0.187825773721066 2202 56-57 0.1293540346699018 2202 58-59 0.09207229913078407 2202 60-61 0.08206217168135055 2202 62-63 0.2491694937429827 2202 64-65 0.1533534849494842 2202 66-67 0.1686568394932877 2202 68-69 0.20505408767372302 2202 70-71 0.1155991230051896 2202 72-73 0.21424876988571384 2202 74-75 0.12417971867782285 2202 76-77 0.12133619597835121 2202 78-79 0.08496614984696294 2202 80-81 0.13246965701335256 2202 82-83 0.14903048018625498 2202 84-85 0.0691805733818498 2202 86-87 0.1649974499394986 2202 88-89 0.17316556749849354 2202 90-91 0.14269382930432073 2202 92-93 0.06039401754579643 2202 94-95 0.1520339087238014 2202 96-97 0.1430179961152831 2202 98-99 0.17021375498865865 2202 100-101 0.2509367264790825 1102 1 0.10205569253257352 1102 2 0.037771718213605254 1102 3 0.12197232927265134 1102 4 0.15642303962224702 1102 5 0.18999647318349133 1102 6 0.1592454848174043 1102 7 0.21829761410977966 1102 8 0.2158639259050119 1102 9 0.15309313176457806 1102 10-11 0.20867520622084612 1102 12-13 0.2575582963551497 1102 14-15 0.2796123443300971 1102 16-17 0.24788279461517249 1102 18-19 0.3140362979980793 1102 20-21 0.2800673902307196 1102 22-23 0.21369119075896492 1102 24-25 0.15949667653802635 1102 26-27 0.25675160988403434 1102 28-29 0.24455856363225337 1102 30-31 0.15254837621477435 1102 32-33 0.15441286079985161 1102 34-35 0.18242030447913749 1102 36-37 0.1510403446459847 1102 38-39 0.12227858823514026 1102 40-41 0.26572524138735787 1102 42-43 0.2302869042531981 1102 44-45 0.16965810853064767 1102 46-47 0.1532833562143665 1102 48-49 0.03844873241532554 1102 50-51 0.09731175290452398 1102 52-53 0.20465437005942277 1102 54-55 0.35184298562409566 1102 56-57 0.35353287334569217 1102 58-59 0.4306555632133069 1102 60-61 0.4412117940207878 1102 62-63 0.4477197155213233 1102 64-65 0.4596981696657778 1102 66-67 0.4024452313631315 1102 68-69 0.280034654313134 1102 70-71 0.3474104719599751 1102 72-73 0.3588306403255004 1102 74-75 0.25881301843209314 1102 76-77 0.3417414642162413 1102 78-79 0.2580659377972907 1102 80-81 0.28308642858662125 1102 82-83 0.3891553137686401 1102 84-85 0.3783431832446382 1102 86-87 0.42160707693719246 1102 88-89 0.3517776884840629 1102 90-91 0.34837146970934896 1102 92-93 0.39957844580882096 1102 94-95 0.501184160470892 1102 96-97 0.4794608588401914 1102 98-99 0.35989038322951217 1102 100-101 0.31972286749770973 2305 1 0.08014128139593879 2305 2 0.07205399667920886 2305 3 0.08214724796804518 2305 4 0.06916071318272543 2305 5 0.10874068590150898 2305 6 0.15950309677830177 2305 7 0.1456408363872157 2305 8 0.28513145096898285 2305 9 0.2006921453827033 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7230.0 15 7383.0 16 7928.0 17 8457.0 18 9492.0 19 10579.0 20 11969.0 21 13644.0 22 15688.0 23 17401.0 24 20494.0 25 23862.0 26 28729.0 27 35395.0 28 42668.0 29 50609.0 30 62005.0 31 81335.0 32 109829.0 33 158290.0 34 240884.0 35 406735.0 36 822083.0 37 1625751.0 38 617008.0 39 635.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.0668138533931 32.80725229142972 16.09109966325461 18.03483419192257 2 30.690578951218512 25.618511130657883 29.93000183771381 13.760908080409791 3 31.939482082097125 23.7723991901276 25.272883789196843 19.015234938578434 4 28.04715044124667 23.294002652764142 29.518790189722356 19.14005671626683 5 29.286352798628062 21.682786848143852 30.603705757577178 18.427154595650908 6 27.43449711798245 22.008821196736857 31.979814840043623 18.57686684523707 7 31.510308178804824 22.743012253284324 25.326002716215644 20.420676851695205 8 27.52179124926987 27.12332778500445 25.116614076769288 20.23826688895639 9 26.023027164691204 24.72819486087383 28.504963650115815 20.743814324319143 10-11 22.794034751090734 29.067298210309467 30.84164872728018 17.297018311319615 12-13 22.833625288792934 28.501138899356338 29.941199869508893 18.724035942341835 14-15 22.20960715039456 28.50519161772863 30.68485539888798 18.60034583298883 16-17 22.342645647386217 28.375738913813684 30.937199094640466 18.34441634415963 18-19 21.906090823618445 28.333014288811874 31.086182802036923 18.674712085532754 20-21 21.979319537742377 29.012855988594637 30.434180966077605 18.573643507585377 22-23 22.226925144944474 28.985563599422697 30.16678948239798 18.62072177323485 24-25 21.544833496574725 29.415733726916738 30.268789747806863 18.770643028701674 26-27 21.454388053482624 29.35398180588651 30.43781113746089 18.753819003169976 28-29 21.166747218179125 29.66052705832395 30.305702767712717 18.867022955784204 30-31 21.1336961294862 29.62080492536789 30.51930290912074 18.72619603602518 32-33 20.605978475327714 29.636102367271906 31.036970895713072 18.720948261687308 34-35 20.59047255601435 30.3022166139364 30.585840212161123 18.521470617888127 36-37 20.7478047438786 29.877384463846703 30.827731891648607 18.547078900626094 38-39 20.888414910355067 29.1992443147146 30.983543429567504 18.928797345362828 40-41 21.034421837141654 29.67272519351311 30.003042069000017 19.289810900345213 42-43 21.396289200498206 29.22961828827005 30.3252859832564 19.048806527975344 44-45 20.80608705557057 29.504686903249006 30.494218704283988 19.195007336896435 46-47 21.08224197220422 29.70717274636524 30.123466057054827 19.08711922437572 48-49 20.793425401510532 29.83911655117283 30.12067571070846 19.246782336608177 50-51 20.89820181344644 29.36442239096722 30.024853729634586 19.712522065951752 52-53 21.38273149453919 29.217011033323377 29.750386068579182 19.649871403558254 54-55 21.47989297576097 29.36975346880103 29.706630341231055 19.443723214206944 56-57 21.437764465350675 29.179830016178563 29.88298603684379 19.49941948162697 58-59 21.372340720184482 29.480312997826207 29.865328378785268 19.282017903204046 60-61 20.80620709998524 29.651746614947534 29.571035022065338 19.971011263001888 62-63 21.04211084146424 29.700373369231333 29.423958651797406 19.833557137507025 64-65 20.682618567022246 29.68583676571757 29.827267336048124 19.804277331212063 66-67 20.90999668774553 29.186433916949746 29.795571585010922 20.107997810293806 68-69 20.757100187030677 29.216637355888675 30.533433827429306 19.492828629651342 70-71 20.736519011847488 30.285315037586933 29.75273857866774 19.225427371897844 72-73 20.653065625938748 30.48067506362127 29.448702731129373 19.41755657931061 74-75 20.813644100844147 30.110031954916806 29.39486164759118 19.681462296647865 76-77 20.950562782846784 29.87545225066813 29.462559153566918 19.71142581291817 78-79 20.653602418209267 29.739972840248235 29.90991107659702 19.696513664945478 80-81 20.659851006427864 30.00792262445197 29.672104278529822 19.66012209059034 82-83 20.350661748577977 30.01458763952739 29.600768626322793 20.033981985571838 84-85 20.384758804588213 30.078698177207315 29.695040515004067 19.841502503200402 86-87 20.47648321018963 29.888894286255084 30.08156909252221 19.553053411033073 88-89 20.681129094152382 30.251585124998492 29.6811389223071 19.386146858542027 90-91 20.953216792876827 29.933745947552215 29.308749646696796 19.804287612874163 92-93 20.599510040310413 30.244587939568884 29.31068113686458 19.845220883256122 94-95 20.177036105004728 30.57230546627025 29.28800381380674 19.962654614918282 96-97 20.395535573554042 30.716083571362553 29.11921524256208 19.769165612521327 98-99 20.62921376416833 30.322398249409954 28.987760920922202 20.060627065499517 100-101 20.623249921362657 30.16433501506141 28.967774490102304 20.244640573473628 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 896.0 1 753.5 2 488.5 3 332.5 4 303.5 5 344.0 6 488.5 7 723.5 8 989.0 9 1281.5 10 1532.5 11 1687.0 12 1759.5 13 1670.0 14 1439.0 15 1250.0 16 1075.5 17 973.0 18 1028.5 19 1174.0 20 1453.5 21 1985.5 22 2892.0 23 4224.5 24 6227.5 25 8995.5 26 13160.5 27 18614.0 28 24933.5 29 32138.0 30 39915.0 31 48269.0 32 61708.5 33 84281.0 34 112436.5 35 148894.0 36 194954.5 37 249760.5 38 300782.0 39 316561.5 40 322103.5 41 309466.5 42 275579.5 43 261904.5 44 245631.5 45 230766.5 46 233078.5 47 215905.5 48 170814.0 49 127843.0 50 97183.0 51 77963.0 52 62055.0 53 46368.5 54 35951.5 55 27836.0 56 19142.5 57 12541.5 58 8258.0 59 5650.0 60 4122.0 61 3096.5 62 2130.5 63 1354.5 64 938.0 65 677.5 66 493.0 67 376.5 68 290.0 69 215.5 70 160.0 71 115.5 72 91.0 73 88.0 74 74.0 75 56.5 76 52.5 77 45.0 78 34.0 79 28.0 80 20.5 81 17.5 82 16.0 83 15.0 84 17.5 85 13.5 86 8.0 87 5.0 88 4.5 89 5.5 90 5.0 91 2.5 92 1.5 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18795586643113568 2 0.19392130605732522 3 0.1645615490911163 4 0.16496221294660665 5 0.17346519032423502 6 0.17061602512963703 7 0.13700477947461395 8 0.11812906006040232 9 0.11846294660664426 10-11 0.09531347940053564 12-13 0.1427810167245997 14-15 0.12035497036868197 16-17 0.11229717505270954 18-19 0.11545796769046668 20-21 0.10751146788990826 22-23 0.10664336286967918 24-25 0.47076890064960963 26-27 0.314242887771383 28-29 0.40470388269986896 30-31 0.26858946734856687 32-33 0.2973482285315403 34-35 0.31718108937831213 36-37 0.2969364351245085 38-39 0.3460845347313237 40-41 0.3411875320531084 42-43 0.3846818194768933 44-45 0.33775963017835775 46-47 0.3617772024046954 48-49 0.3570916612057667 50-51 0.372995790358425 52-53 0.363524541996695 54-55 0.33923986053336375 56-57 0.3548434917944042 58-59 0.34679682602997325 60-61 0.3172589962391019 62-63 0.326563301327711 64-65 0.3278431997549718 66-67 0.3390283990540771 68-69 0.32651878312154536 70-71 0.3144320901475867 72-73 0.3004867620662146 74-75 0.30919007137158816 76-77 0.2986837747165081 78-79 0.30846665052139727 80-81 0.31674703686819766 82-83 0.3442592882785344 84-85 0.34224483944954126 86-87 0.3350328900507152 88-89 0.34746459912245714 90-91 0.3373923549774916 92-93 0.3209873960054704 94-95 0.3133859123026953 96-97 0.2983164995156419 98-99 0.32300184483446354 100-101 0.3274202767963987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4492544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.67321563364919 #Duplication Level Percentage of deduplicated Percentage of total 1 90.05760838799156 44.73451000907439 2 4.654709101919123 4.624287378630764 3 1.5231219656543087 2.2697509750888227 4 0.7773498960877465 1.544538760446457 5 0.5007118026835627 1.2435982672506907 6 0.30103074122682016 0.8971898952790254 7 0.26474333394424326 0.9205456900208514 8 0.20643296822236468 0.8203351475523016 9 0.161035069144217 0.7199216742762268 >10 1.2557959003182273 12.748893619358242 >50 0.15890510760205764 5.473307680202142 >100 0.11139883308883146 10.704546214551103 >500 0.0175698883528048 6.165737274816766 >1k 0.009541726469445304 6.916949297047021 >5k 4.5277294719092935E-5 0.21588811640518088 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 9599 0.21366513049176591 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.34882329477463E-4 0.0 0.0 2 0.0010906960510570403 0.0 0.0 3 0.001624914525044162 0.0 0.0 4 0.0022036512051968773 0.0 0.0 5 0.0028046469884323894 0.0 0.0 6 0.005230889224457234 0.0 0.0 7 0.006477398997093852 0.0 0.0 8 0.008280386346800388 0.0 0.0 9 0.009304305088609037 0.0 2.2259103082796742E-5 10-11 0.012698818308735541 0.0 2.2259103082796742E-5 12-13 0.01876442389879765 0.0 2.2259103082796742E-5 14-15 0.026844478317852867 0.0 2.2259103082796742E-5 16-17 0.03706140663285657 0.0 2.2259103082796742E-5 18-19 0.04909245184910821 0.0 2.2259103082796742E-5 20-21 0.06333827782209812 0.0 2.2259103082796742E-5 22-23 0.08108991253062853 0.0 2.2259103082796742E-5 24-25 0.10026612983645791 0.0 3.3388654624195115E-5 26-27 0.12185745982677076 0.0 4.4518206165593484E-5 28-29 0.143292976095504 0.0 6.677730924839022E-5 30-31 0.16774460083195625 0.0 6.677730924839022E-5 32-33 0.19163974799133854 0.0 6.677730924839022E-5 34-35 0.2165031661348225 0.0 7.790686078978859E-5 36-37 0.24561807296712063 0.0 1.1129551541398371E-4 38-39 0.27700340831386405 0.0 1.1129551541398371E-4 40-41 0.31269588010712857 0.0 1.1129551541398371E-4 42-43 0.3526398405892074 0.0 1.1129551541398371E-4 44-45 0.39574459370904325 0.0 1.1129551541398371E-4 46-47 0.44138688458031794 0.0 1.1129551541398371E-4 48-49 0.4898115633369423 0.0 1.1129551541398371E-4 50-51 0.5439902202404696 0.0 1.1129551541398371E-4 52-53 0.5995044233289646 0.0 1.2242506695538206E-4 54-55 0.6605945317397003 0.0 1.3355461849678043E-4 56-57 0.7221743404182575 0.0 1.3355461849678043E-4 58-59 0.7899310502022907 0.0 1.3355461849678043E-4 60-61 0.8596576906091515 0.0 1.3355461849678043E-4 62-63 0.9320220347313237 0.0 1.3355461849678043E-4 64-65 1.0080257422075332 0.0 1.7807282466237394E-4 66-67 1.0865002101259331 0.0 1.7807282466237394E-4 68-69 1.1668889609094535 0.0 1.892023762037723E-4 70-71 1.2522081030258134 0.0 2.0033192774517066E-4 72-73 1.3406323900222235 0.0 2.0033192774517066E-4 74-75 1.4321284332440594 0.0 2.2259103082796741E-4 76-77 1.5298570253290786 0.0 2.2259103082796741E-4 78-79 1.6355321172146562 0.0 2.2259103082796741E-4 80-81 1.7451693294489714 0.0 2.2259103082796741E-4 82-83 1.8612505520257563 0.0 2.2259103082796741E-4 84-85 1.9830634936463616 0.0 2.2259103082796741E-4 86-87 2.1111089841301496 0.0 2.337205823693658E-4 88-89 2.245843335090319 0.0 2.4485013391076417E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCC 85 0.0 61.368034 1 CGGACGG 55 1.1999873E-8 60.353687 1 GAGCGGC 515 0.0 58.901085 9 CGTTGCG 25 0.005055523 56.904907 1 ATCGGGA 725 0.0 54.93899 2 GTCGCGC 35 2.8119102E-4 54.19515 1 CGGGAGA 720 0.0 49.386154 4 GAGAGCG 830 0.0 45.684334 7 AGAGCGG 695 0.0 45.01057 8 CGGTGCG 45 9.7075076E-4 42.15178 1 CGACGGG 115 5.638867E-11 41.23544 1 CGAGGCG 160 0.0 38.529366 1 GATCCGG 215 0.0 37.49548 1 GGTCCGG 145 1.6370905E-11 35.974365 1 CGCGAGG 55 0.0026016354 34.48782 1 CGCGTGA 55 0.0026016354 34.48782 1 GATCGGG 1440 0.0 33.5897 1 CGGATCC 130 0.0 32.884945 60-61 CACGACG 65 8.507413E-9 32.80177 14-15 GTGTTTG 60795 0.0 32.120842 1 >>END_MODULE