FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34274-2.3420000003583a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34274-2.3420000003583a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4492544
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT95990.21366513049176591Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC850.061.3680341
CGGACGG551.1999873E-860.3536871
GAGCGGC5150.058.9010859
CGTTGCG250.00505552356.9049071
ATCGGGA7250.054.938992
GTCGCGC352.8119102E-454.195151
CGGGAGA7200.049.3861544
GAGAGCG8300.045.6843347
AGAGCGG6950.045.010578
CGGTGCG459.7075076E-442.151781
CGACGGG1155.638867E-1141.235441
CGAGGCG1600.038.5293661
GATCCGG2150.037.495481
GGTCCGG1451.6370905E-1135.9743651
CGCGAGG550.002601635434.487821
CGCGTGA550.002601635434.487821
GATCGGG14400.033.58971
CGGATCC1300.032.88494560-61
CACGACG658.507413E-932.8017714-15
GTGTTTG607950.032.1208421
Produced by FastQC (version 0.11.2)