##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34271-1.342000000358da.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5067364 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.226091908929376 33.0 33.0 33.0 27.0 33.0 2 30.796096155713307 33.0 33.0 33.0 27.0 33.0 3 30.663920728804957 33.0 33.0 33.0 27.0 33.0 4 34.09389457714109 37.0 37.0 37.0 27.0 37.0 5 34.17495151325225 37.0 37.0 37.0 27.0 37.0 6 34.643104580606405 37.0 37.0 37.0 27.0 37.0 7 34.04598603928986 37.0 37.0 37.0 27.0 37.0 8 34.33867924230428 37.0 37.0 37.0 27.0 37.0 9 33.87341308025238 37.0 37.0 37.0 27.0 37.0 10-11 33.85646274867959 37.0 37.0 37.0 24.5 37.0 12-13 33.83203032187939 37.0 37.0 37.0 24.5 37.0 14-15 35.365835471854794 40.0 37.0 40.0 22.0 40.0 16-17 35.30158470952551 40.0 37.0 40.0 22.0 40.0 18-19 35.333606585198936 40.0 37.0 40.0 22.0 40.0 20-21 35.344134938796586 40.0 37.0 40.0 22.0 40.0 22-23 35.970802373778554 40.0 37.0 40.0 27.0 40.0 24-25 35.29461234677438 40.0 37.0 40.0 22.0 40.0 26-27 35.20395179821304 40.0 37.0 40.0 21.0 40.0 28-29 34.898004268096784 40.0 37.0 40.0 18.5 40.0 30-31 34.88321008713801 40.0 37.0 40.0 18.5 40.0 32-33 34.762211181197955 37.0 37.0 40.0 18.5 40.0 34-35 34.787662382256336 37.0 37.0 40.0 18.5 40.0 36-37 34.614752759028164 37.0 37.0 40.0 15.0 40.0 38-39 34.536837002433614 37.0 37.0 40.0 15.0 40.0 40-41 34.50087619519735 37.0 37.0 40.0 18.5 40.0 42-43 34.189603411162096 37.0 37.0 40.0 15.0 40.0 44-45 33.99380397776832 37.0 33.0 40.0 15.0 40.0 46-47 33.94960348220495 37.0 33.0 40.0 15.0 40.0 48-49 33.849633458342446 37.0 33.0 40.0 15.0 40.0 50-51 32.80527449774676 37.0 33.0 38.5 15.0 38.5 52-53 33.258296621280806 37.0 33.0 38.5 15.0 40.0 54-55 33.918515030694465 37.0 33.0 40.0 15.0 40.0 56-57 34.08506681185721 37.0 33.0 40.0 18.5 40.0 58-59 33.63640346341806 37.0 33.0 40.0 15.0 40.0 60-61 33.411745436088665 37.0 33.0 40.0 15.0 40.0 62-63 33.115987523296134 37.0 33.0 40.0 15.0 40.0 64-65 32.92624676261662 37.0 33.0 38.5 15.0 40.0 66-67 32.781755859654055 37.0 33.0 37.0 15.0 40.0 68-69 32.76225548431097 37.0 33.0 37.0 15.0 40.0 70-71 32.38172657421097 37.0 33.0 37.0 15.0 40.0 72-73 32.42963175726078 37.0 33.0 37.0 15.0 38.5 74-75 32.454483928922414 37.0 33.0 37.0 22.0 37.0 76-77 32.212339295144375 37.0 33.0 37.0 22.0 37.0 78-79 31.87722561473776 37.0 33.0 37.0 18.5 37.0 80-81 31.502298729674834 37.0 33.0 37.0 15.0 37.0 82-83 31.180839584446666 33.0 33.0 37.0 15.0 37.0 84-85 30.970711991481174 33.0 33.0 37.0 15.0 37.0 86-87 30.77881626423521 33.0 33.0 37.0 15.0 37.0 88-89 30.620098931120793 33.0 30.0 37.0 15.0 37.0 90-91 30.476796910583097 33.0 27.0 37.0 15.0 37.0 92-93 30.354626685590375 33.0 27.0 37.0 15.0 37.0 94-95 30.210732443929427 33.0 27.0 37.0 15.0 37.0 96-97 30.035948769419367 33.0 27.0 37.0 15.0 37.0 98-99 29.883168250790746 33.0 27.0 37.0 15.0 37.0 100-101 28.678443664200955 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.08940679276504682 2306 2 -0.27358799954434687 2306 3 -0.32430850604533035 2306 4 -0.20426651246155814 2306 5 -0.2648910071310553 2306 6 -0.23271260644199998 2306 7 0.1801891412796479 2306 8 -0.37737958283765494 2306 9 -0.16349914472686322 2306 10-11 -0.1704799961061454 2306 12-13 -0.17697040944233322 2306 14-15 0.15343457023424634 2306 16-17 -0.017170686755463294 2306 18-19 -0.0691966372816637 2306 20-21 -0.09861128525427887 2306 22-23 -0.09494294346045251 2306 24-25 -0.02227251206095815 2306 26-27 -0.15453596737928876 2306 28-29 -0.15750248789686339 2306 30-31 -0.14763041497000273 2306 32-33 -0.15641849850221945 2306 34-35 -0.25232980234841307 2306 36-37 -0.20609312597547813 2306 38-39 -0.15027454764029358 2306 40-41 -0.24215541270174157 2306 42-43 -0.04249327527109159 2306 44-45 -0.06857873200516451 2306 46-47 -0.11358140250356996 2306 48-49 0.0025210220192306565 2306 50-51 -0.030795949001444 2306 52-53 -0.10127611395797231 2306 54-55 0.06827360155687501 2306 56-57 0.050982635055291325 2306 58-59 0.2075018376870048 2306 60-61 0.09853387178432627 2306 62-63 0.13230348462233366 2306 64-65 0.06562585966216972 2306 66-67 0.18484174571019452 2306 68-69 0.015959763085305667 2306 70-71 0.09803352178131775 2306 72-73 -0.022096326662108368 2306 74-75 -0.08508837397933888 2306 76-77 9.295750518276691E-4 2306 78-79 -0.08086252469523458 2306 80-81 0.04776105817561316 2306 82-83 0.01603278949763265 2306 84-85 -0.015301946635958075 2306 86-87 0.13937283334556483 2306 88-89 0.0792708539802014 2306 90-91 0.1562123989931763 2306 92-93 0.05551425236233953 2306 94-95 0.06368606480885575 2306 96-97 0.07383128237802339 2306 98-99 -0.07580083128683412 2306 100-101 -0.10205325021986056 2307 1 0.10904365975533636 2307 2 0.07547860573514242 2307 3 0.05472295790982784 2307 4 0.16807041344771534 2307 5 0.10993791666112429 2307 6 0.023675239952517302 2307 7 0.4491830695762289 2307 8 -0.010890239529977919 2307 9 0.14833131354929918 2307 10-11 0.2890346178316392 2307 12-13 0.22415475914283434 2307 14-15 0.493386843269235 2307 16-17 0.42094804496831983 2307 18-19 0.5077701822863432 2307 20-21 0.3995021141669781 2307 22-23 0.2840811938905219 2307 24-25 0.44841792074206666 2307 26-27 0.30320073615289544 2307 28-29 0.4882116537973147 2307 30-31 0.5412474628468544 2307 32-33 0.36150691783939237 2307 34-35 0.22577922015950946 2307 36-37 0.2279895626655275 2307 38-39 0.32869014897089244 2307 40-41 0.33799858958767715 2307 42-43 0.4541019418630796 2307 44-45 0.3836659123937025 2307 46-47 0.3782811946545266 2307 48-49 0.298346879774094 2307 50-51 0.2073557964008188 2307 52-53 0.26379908484209835 2307 54-55 0.3507086248349651 2307 56-57 0.3777086291436049 2307 58-59 0.554939152726881 2307 60-61 0.42416856817863646 2307 62-63 0.45239454258984324 2307 64-65 0.42929343463237757 2307 66-67 0.38417732528268544 2307 68-69 0.3847665112980536 2307 70-71 0.31961497955283136 2307 72-73 0.21983323565828528 2307 74-75 0.15117845596314794 2307 76-77 0.14054673653780725 2307 78-79 0.19090118968208003 2307 80-81 0.2042075393834608 2307 82-83 0.1724854616860192 2307 84-85 0.2733318600155421 2307 86-87 0.4357197225573479 2307 88-89 0.44878084629606363 2307 90-91 0.4162254857735803 2307 92-93 0.4391927615566402 2307 94-95 0.5205203505030411 2307 96-97 0.48552570026759767 2307 98-99 0.45578892166593477 2307 100-101 0.23586548718937195 2201 1 0.18803872005392464 2201 2 0.015181134280570774 2201 3 -0.020925374619384485 2201 4 0.08758219465396166 2201 5 -0.010471177135450205 2201 6 0.04449263742444032 2201 7 0.3494332833814582 2201 8 0.0672732247394805 2201 9 0.21700997623369034 2201 10-11 0.17834746042530725 2201 12-13 0.23045981497369183 2201 14-15 0.37065315901007523 2201 16-17 0.4232769666602181 2201 18-19 0.44941267500594506 2201 20-21 0.319261103567797 2201 22-23 0.3703709631068719 2201 24-25 0.36887522243344506 2201 26-27 0.26182981807403394 2201 28-29 0.2314674047511076 2201 30-31 0.3638974690680712 2201 32-33 0.31954741194603287 2201 34-35 0.2236081914636685 2201 36-37 0.16445712228293274 2201 38-39 0.24472798895270387 2201 40-41 0.20652103926797594 2201 42-43 0.3582015357250228 2201 44-45 0.3126451374554051 2201 46-47 0.2517371442148715 2201 48-49 0.2641583867741417 2201 50-51 0.1520514776664399 2201 52-53 0.22941434121675286 2201 54-55 0.2991696209337107 2201 56-57 0.3768418254700876 2201 58-59 0.43478291084793597 2201 60-61 0.3979986572024643 2201 62-63 0.3917468978073444 2201 64-65 0.46155938681246056 2201 66-67 0.4276709394756679 2201 68-69 0.31187362811157016 2201 70-71 0.37160636723145046 2201 72-73 0.2302347813034018 2201 74-75 0.24910377743223933 2201 76-77 0.22929946586852168 2201 78-79 0.12498969034699314 2201 80-81 0.2973773679350309 2201 82-83 0.3554782597704076 2201 84-85 0.32988770714887394 2201 86-87 0.33845373231494946 2201 88-89 0.32987846249244157 2201 90-91 0.28402143736041197 2201 92-93 0.3196951290328336 2201 94-95 0.3219303362703627 2201 96-97 0.2116098988405497 2201 98-99 0.120677968695734 2201 100-101 0.16683021995777025 1101 1 0.049512687264488875 1101 2 -0.002168751858818041 1101 3 0.07023496143493801 1101 4 0.12097278874474426 1101 5 0.11416887229667338 1101 6 0.16577411754951754 1101 7 0.3589034266285225 1101 8 0.22962665303273155 1101 9 0.28528944938302203 1101 10-11 0.20477852554061116 1101 12-13 0.11565372931895013 1101 14-15 0.34471530482039014 1101 16-17 0.39743343087218364 1101 18-19 0.34627454815941405 1101 20-21 0.3031814463169411 1101 22-23 0.250477000371653 1101 24-25 0.1804515828287947 1101 26-27 0.09522301465948857 1101 28-29 0.11567148991577625 1101 30-31 0.33743280063558956 1101 32-33 0.3924527753115612 1101 34-35 0.20137113756753422 1101 36-37 0.11612550641984143 1101 38-39 0.24763156229538197 1101 40-41 0.18460703544389645 1101 42-43 0.1393878080179789 1101 44-45 0.1700160632661536 1101 46-47 0.1587244253005693 1101 48-49 0.1494846876146525 1101 50-51 0.1537729133340946 1101 52-53 0.16704887565131088 1101 54-55 0.433024763260498 1101 56-57 0.35972669257454726 1101 58-59 0.23317114418819784 1101 60-61 0.36406932321609986 1101 62-63 0.4677956987534557 1101 64-65 0.4128906509569177 1101 66-67 0.29005616167672343 1101 68-69 0.3080277519582424 1101 70-71 0.32558445070150555 1101 72-73 0.2169368559968774 1101 74-75 0.23574645885005907 1101 76-77 0.07266244942950806 1101 78-79 -0.037552815352498214 1101 80-81 0.19306112758695093 1101 82-83 0.2708668401295391 1101 84-85 0.25525964505393617 1101 86-87 0.21509536744766322 1101 88-89 0.3568695339383261 1101 90-91 0.3511530517430792 1101 92-93 0.36115765701626046 1101 94-95 0.24469860456394343 1101 96-97 0.31724233765005394 1101 98-99 0.3141788227898381 1101 100-101 0.06149971165296364 2304 1 0.1279889077273637 2304 2 -0.06976228151403063 2304 3 -0.011898530809432373 2304 4 0.10176030731749819 2304 5 -0.04938699389804668 2304 6 0.11512192286182454 2304 7 0.32171676362985835 2304 8 0.04129040960351915 2304 9 0.11217874603052991 2304 10-11 0.12529757482379722 2304 12-13 0.1387440134405722 2304 14-15 0.2724140840610616 2304 16-17 0.17510513739458133 2304 18-19 0.24412170499815744 2304 20-21 0.23068767685180092 2304 22-23 0.18000868124700986 2304 24-25 0.07137999811887141 2304 26-27 0.15822657701176013 2304 28-29 0.12470931354287984 2304 30-31 0.15169190020518641 2304 32-33 0.04944570056183295 2304 34-35 -7.67497178706833E-4 2304 36-37 0.051756127979508904 2304 38-39 0.013199797215854403 2304 40-41 -0.003693123547620303 2304 42-43 0.1405347297364301 2304 44-45 0.14098244421382589 2304 46-47 0.09198216643194002 2304 48-49 0.14679364707737363 2304 50-51 0.04626175921855946 2304 52-53 0.06830707158074034 2304 54-55 0.2490117305810884 2304 56-57 0.214038418010027 2304 58-59 0.3306931601219034 2304 60-61 0.42814248732441484 2304 62-63 0.33523982542233455 2304 64-65 0.2777500319153816 2304 66-67 0.2423233455554552 2304 68-69 0.24811768564497072 2304 70-71 0.24413275311516713 2304 72-73 0.20179483328412573 2304 74-75 0.21264624815449906 2304 76-77 0.24424505062435742 2304 78-79 0.05436975436598601 2304 80-81 0.07956846147391516 2304 82-83 0.14677532956629236 2304 84-85 0.10169313166966987 2304 86-87 0.15662511044115135 2304 88-89 0.24911169104615638 2304 90-91 0.14451174988739623 2304 92-93 0.3196238253474526 2304 94-95 0.363882980372658 2304 96-97 0.24993171535206216 2304 98-99 0.18983144807621244 2304 100-101 0.18326065045574325 2202 1 -0.011366550105194051 2202 2 -0.10083825721165596 2202 3 -0.021431187289302045 2202 4 -0.10044313101277424 2202 5 -0.11556057886052429 2202 6 -0.013552172903395387 2202 7 0.13840983188434564 2202 8 0.021022846545939444 2202 9 -0.03260263807376873 2202 10-11 0.0015443483460231278 2202 12-13 0.03365538291151182 2202 14-15 0.07556591405244006 2202 16-17 0.04656936082012919 2202 18-19 -0.05613941488651619 2202 20-21 -0.07870649698043053 2202 22-23 0.10812913866275409 2202 24-25 0.028882175934100474 2202 26-27 -0.013780927541134247 2202 28-29 0.0293282135860764 2202 30-31 0.04538893367585928 2202 32-33 -0.012491924194776516 2202 34-35 -0.021220162766596218 2202 36-37 -0.05386604065637357 2202 38-39 -0.13857085435941485 2202 40-41 -0.040349492265917775 2202 42-43 0.010122319614801256 2202 44-45 0.06869559681107518 2202 46-47 0.12429125090452686 2202 48-49 0.06647264649938478 2202 50-51 0.02874576979253618 2202 52-53 0.049189848679404236 2202 54-55 0.22842572380733372 2202 56-57 0.22091388482181173 2202 58-59 0.2916320683121896 2202 60-61 0.306542607073915 2202 62-63 0.2903546412477951 2202 64-65 0.18535174753895234 2202 66-67 0.17629169564798275 2202 68-69 0.23965960953539422 2202 70-71 0.19212709017642737 2202 72-73 0.1751527739771177 2202 74-75 0.19401895009320924 2202 76-77 0.24668996924784636 2202 78-79 0.17595160482761685 2202 80-81 0.062140961657309646 2202 82-83 0.15890677702994438 2202 84-85 0.18715571785168805 2202 86-87 0.25627359890340173 2202 88-89 0.34928267189292583 2202 90-91 0.26577560242432696 2202 92-93 0.15838991216653397 2202 94-95 0.2800335154388378 2202 96-97 0.14577521004821037 2202 98-99 0.16546101833668914 2202 100-101 0.2672275814453897 1102 1 -0.026072236216702294 1102 2 -0.1651033152594117 1102 3 -0.11965622331232595 1102 4 -0.1153064192962745 1102 5 -0.15024713736010398 1102 6 -0.0035401785728410573 1102 7 -0.07683327533289486 1102 8 0.026849341325466014 1102 9 -0.17099901825788066 1102 10-11 -0.02217020002321135 1102 12-13 -0.008954584521660536 1102 14-15 -0.05200082713130172 1102 16-17 -0.02170829454179568 1102 18-19 -0.009553018112072209 1102 20-21 -0.04083108285483661 1102 22-23 0.009714841987637612 1102 24-25 -0.06893985868094887 1102 26-27 0.047374421080853324 1102 28-29 -0.08364790257551391 1102 30-31 -0.08868024304812394 1102 32-33 -0.08638555464662545 1102 34-35 -0.18478745042723688 1102 36-37 -0.1725751946598777 1102 38-39 -0.13453920673891417 1102 40-41 -0.13131116185316927 1102 42-43 -0.09865479152495737 1102 44-45 -0.08740926251714143 1102 46-47 -0.02745476949107939 1102 48-49 -0.14533307682004448 1102 50-51 -0.11518481641299871 1102 52-53 -0.03978175412409968 1102 54-55 0.14041781802224307 1102 56-57 0.16600687562511496 1102 58-59 0.20298181288622885 1102 60-61 0.3247085466538948 1102 62-63 0.23168158435372987 1102 64-65 0.27713971265976767 1102 66-67 0.24316807213615022 1102 68-69 0.08475243468384974 1102 70-71 0.1332546952943119 1102 72-73 0.11351309202336068 1102 74-75 0.07042095370687917 1102 76-77 0.11400262996179578 1102 78-79 0.11639319184881458 1102 80-81 -0.007292642365232638 1102 82-83 0.2501773468743025 1102 84-85 0.14963478207119252 1102 86-87 0.19877995843863872 1102 88-89 0.25093631670888783 1102 90-91 0.26821473186398137 1102 92-93 0.20344455387490612 1102 94-95 0.29457131497384736 1102 96-97 0.10984681545617647 1102 98-99 0.11064827134417854 1102 100-101 0.18614084981809498 2305 1 0.27196783393917556 2305 2 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11 69713.0 12 47920.0 13 33892.0 14 25963.0 15 21346.0 16 19111.0 17 18237.0 18 18141.0 19 18815.0 20 19373.0 21 20831.0 22 22293.0 23 24397.0 24 27463.0 25 32608.0 26 38564.0 27 45039.0 28 52968.0 29 63388.0 30 78248.0 31 102160.0 32 137966.0 33 196687.0 34 296471.0 35 496065.0 36 930325.0 37 1507474.0 38 453248.0 39 1191.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.633926223787974 23.723901650168933 21.5639932229794 15.078178903063696 2 31.654514680741734 26.41388796131926 29.19034934809484 12.741248009844167 3 35.30996430885314 20.323912277809864 28.573670456355728 15.792452956981279 4 28.98862382405301 18.525040256487237 32.231062255395024 20.255273664064728 5 34.472222370471314 17.11980483303891 30.737373180316553 17.670599616173224 6 31.335846252068016 17.415574429898857 34.809208588726655 16.43937072930647 7 35.2987437384023 19.424680915640167 27.231851666403532 18.044723679554 8 28.665821099277117 29.796016745395313 24.046672158903448 17.49148999642412 9 32.40520073517253 25.23402853880805 24.046870706085286 18.313900019934138 10-11 27.023914716191566 28.818947998205314 27.740278717791888 16.416858567811232 12-13 28.482438686721757 24.19798372778171 27.39942895275532 19.920148632741213 14-15 25.466819781441007 24.540534666913626 28.99945421991727 20.9931913317281 16-17 27.298174723893272 24.853018497923674 29.66663120599951 18.182175572183546 18-19 26.879625999506302 24.9398362621761 29.837598654157706 18.34293908415989 20-21 25.584124195951404 27.285273934582516 28.58650814251686 18.544093726949225 22-23 28.526508664391827 25.111589670427858 27.57822240576178 18.783679259418538 24-25 24.570648558076982 28.613203268046934 27.84368138207649 18.97246679179959 26-27 23.278626058082253 30.034299061489396 27.788643353598037 18.89843152683032 28-29 24.404570967548278 29.158215542644882 27.26957878679867 19.16763470300817 30-31 26.749952833460018 26.439459977496586 27.832720489847397 18.977866699195996 32-33 23.228034806297465 26.242744523403523 31.533397532873238 18.995823137425774 34-35 21.095298025011147 28.73585767529653 28.894759477170112 21.27408482252221 36-37 23.737240140528048 29.670147408780323 27.788747563218273 18.803864887473356 38-39 25.049459602727165 26.953597377943044 27.491737261243927 20.50520575808586 40-41 23.46424411510354 26.278549538173056 27.12111949286407 23.136086853859332 42-43 25.86803935749607 26.130410779156954 28.312926997355685 19.688622865991295 44-45 23.635518172086435 27.917933336824753 28.907938345260085 19.538610145828727 46-47 24.509705480667463 28.44525155110241 27.603216042106332 19.4418269261238 48-49 21.64795239480562 28.385357616238966 29.211542928821338 20.755147060134078 50-51 20.70463452858731 27.0168728630389 30.249361851731727 22.029130756642065 52-53 25.638561690289297 26.37341798537734 27.386535163808023 20.60148516052534 54-55 26.44920172046034 26.262513260108 27.711192743930297 19.57709227550136 56-57 27.145830486590107 26.134677194170663 27.090658127427158 19.62883419181207 58-59 25.763018557671113 26.364360210144827 26.9861373402711 20.886483891912956 60-61 22.8258205694476 26.474118933574474 26.68908512574107 24.010975371236853 62-63 25.189663760600776 26.426289297816453 27.07859008949437 21.3054568520884 64-65 23.018239988596296 27.488819023597355 28.69884913650591 20.79409185130044 66-67 21.688515787085127 27.655109680551654 27.69257210427543 22.96380242808779 68-69 21.302403880086434 26.660666560219553 31.637729120733297 20.399200438960712 70-71 21.083321992126315 29.834850580628558 28.962836308028834 20.118991119216293 72-73 20.675210587042958 32.82559086938403 26.47231806018746 20.02688048338555 74-75 20.834977096840156 33.04299174789481 26.1139372076732 20.00809394759184 76-77 20.736766800525725 32.773493115770385 26.152046723438428 20.33769336026546 78-79 20.692186117025493 32.43710523291944 26.5761907207919 20.29451792926316 80-81 20.529306752882096 32.360479949449 26.642877843790757 20.46733545387815 82-83 20.17882313431853 32.2496470674196 26.813193297553127 20.758336500708737 84-85 20.337951559121475 31.898538298866598 26.82057232470279 20.94293781730914 86-87 20.224205311296057 31.69791513631862 27.33573516303231 20.742144389353015 88-89 20.321024197805844 31.64340642563251 27.390315016231803 20.64525436032984 90-91 20.679605614348553 31.17529434779372 27.04758431264464 21.09751572521309 92-93 20.478523989301465 31.470120188769506 26.861140264509725 21.190215557419307 94-95 20.497418247642948 31.3401446976956 26.792382650252012 21.37005440440944 96-97 20.7772662232461 31.4719084122214 26.647223350109634 21.10360201442286 98-99 20.970169161684797 31.147111125848987 26.659354919814128 21.22336479265209 100-101 21.012886329289323 31.25441925397499 26.22316882399018 21.50952559274551 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2363.0 1 1749.5 2 948.0 3 830.5 4 858.0 5 840.5 6 935.0 7 1127.0 8 1389.5 9 1696.5 10 2022.0 11 2276.5 12 2382.0 13 2317.0 14 2160.0 15 2036.5 16 1942.5 17 1837.0 18 1855.5 19 1925.0 20 2038.0 21 2320.0 22 2732.5 23 3487.5 24 4610.5 25 6314.5 26 9472.5 27 13912.5 28 18652.5 29 23351.5 30 28647.5 31 34013.0 32 44307.5 33 63966.0 34 92780.5 35 123823.5 36 161769.0 37 210120.5 38 247699.0 39 258149.0 40 259155.0 41 257922.5 42 257964.0 43 266117.5 44 264723.5 45 255691.0 46 245548.0 47 224627.0 48 198111.5 49 179578.5 50 164645.0 51 152917.5 52 142936.5 53 128000.5 54 110104.5 55 93546.5 56 79452.0 57 68569.5 58 59749.5 59 51028.0 60 42427.0 61 34867.0 62 28599.0 63 23427.0 64 19232.5 65 15435.0 66 12159.0 67 9972.5 68 8646.5 69 6774.0 70 4823.5 71 3660.5 72 2774.5 73 2135.5 74 1633.5 75 1313.0 76 1065.5 77 800.5 78 597.0 79 428.0 80 309.0 81 253.5 82 222.5 83 186.0 84 137.5 85 105.0 86 101.0 87 87.5 88 65.5 89 57.5 90 47.0 91 33.0 92 31.0 93 26.5 94 16.5 95 15.5 96 13.0 97 7.5 98 6.5 99 4.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17946214244723688 2 0.1843759398377539 3 0.15483395311645265 4 0.156511353832091 5 0.16401032173729774 6 0.16130674646621004 7 0.1293571963648161 8 0.11197143130037629 9 0.11238584794776929 10-11 0.09165514851508595 12-13 0.13492221991552217 14-15 0.1144875323738338 16-17 0.10690962796436176 18-19 0.10957373498331677 20-21 0.10202543176294421 22-23 0.10221290596057438 24-25 0.4564108676621612 26-27 0.30541520206561046 28-29 0.39872801717026846 30-31 0.2656904062940811 32-33 0.29379180181254 34-35 0.31212480492816386 36-37 0.2930419050220194 38-39 0.3410649008044419 40-41 0.33629910935942237 42-43 0.37844133557407755 44-45 0.333200851567008 46-47 0.35624044374945235 48-49 0.3518792018887927 50-51 0.3665909928712443 52-53 0.35808558453665457 54-55 0.33491772053477903 56-57 0.3499649916603583 58-59 0.3418542658470952 60-61 0.31322004892484534 62-63 0.32206093740256275 64-65 0.32312658021014473 66-67 0.3348881193456795 68-69 0.32250495523905526 70-71 0.31034873358219384 72-73 0.2960020239319694 74-75 0.3047146405902556 76-77 0.2942456867120657 78-79 0.30417195212343145 80-81 0.31243068388219203 82-83 0.3390026846305101 84-85 0.33713780971724155 86-87 0.33094129413241286 88-89 0.3434132618063356 90-91 0.3330331114954442 92-93 0.3161110983935632 94-95 0.30902654713574945 96-97 0.29402861132533603 98-99 0.3183410546390589 100-101 0.32193266558313155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5067364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.783717583846474 #Duplication Level Percentage of deduplicated Percentage of total 1 92.03574340527922 56.86310378171133 2 4.12602537465921 5.09842372983458 3 1.315812284094181 2.4388732366149255 4 0.580145061075787 1.4337407444467918 5 0.4069211182502097 1.2570549724436977 6 0.17609574169299358 0.6527909743486737 7 0.1950117002709333 0.8433983470559544 8 0.14191399583828251 0.701437939205409 9 0.10057297447842999 0.5592395026568447 >10 0.7242686137814144 8.726513747409136 >50 0.09934352914779594 4.276627717271511 >100 0.08681139481540419 11.30375427528924 >500 0.009576527715790168 3.939182183726739 >1k 0.0017262690456891112 1.6199247435971071 >5k 0.0 0.0 >10k+ 3.20098545863023E-5 0.2859341043881365 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 14458 0.28531599466704977 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002387829254026354 0.0 0.0 2 0.0028811824056846915 0.0 0.0 3 0.003275864927011361 0.0 0.0 4 0.0037889522047360323 0.0 0.0 5 0.00428230535639437 0.0 0.0 6 0.005051936272981376 0.0 0.0 7 0.006176781458762386 0.0 0.0 8 0.007025348879614726 0.0 0.0 9 0.007696309165870066 0.0 0.0 10-11 0.011791140324634268 0.0 0.0 12-13 0.015224878260176298 0.0 0.0 14-15 0.01919143759950933 0.0 0.0 16-17 0.022487036652587028 0.0 0.0 18-19 0.026591734874384394 0.0 0.0 20-21 0.03143646282366927 0.0 0.0 22-23 0.03635026021418631 0.0 0.0 24-25 0.04203368852129036 0.0 0.0 26-27 0.047677648576261736 0.0 0.0 28-29 0.05458459269947846 0.0 0.0 30-31 0.08165981366248803 0.0 0.0 32-33 0.09014548787101143 0.0 0.0 34-35 0.09797993591934584 0.0 0.0 36-37 0.10621893355204007 0.0 0.0 38-39 0.11616493308947216 0.0 0.0 40-41 0.12632800801363392 0.0 0.0 42-43 0.13863223561599286 0.0 9.86706303316675E-6 44-45 0.15136074692877793 0.0 1.97341260663335E-5 46-47 0.1646714149605199 0.0 1.97341260663335E-5 48-49 0.1797186860860992 0.0 1.97341260663335E-5 50-51 0.19547638575006648 0.0 2.960118909950025E-5 52-53 0.21181624213299063 0.0 3.9468252132667E-5 54-55 0.22969536034908877 0.0 3.9468252132667E-5 56-57 0.24857105193153678 0.0 3.9468252132667E-5 58-59 0.2703772612348353 0.0 3.9468252132667E-5 60-61 0.2926176213115932 0.0 3.9468252132667E-5 62-63 0.3164564455997241 0.0 4.933531516583375E-5 64-65 0.34259429557458276 0.0 5.9202378199000505E-5 66-67 0.37186000453095536 0.0 5.9202378199000505E-5 68-69 0.40263537413140243 1.97341260663335E-5 5.9202378199000505E-5 70-71 0.43581830711194225 1.97341260663335E-5 5.9202378199000505E-5 72-73 0.47260271809958787 1.97341260663335E-5 5.9202378199000505E-5 74-75 0.5131958154180358 1.97341260663335E-5 5.9202378199000505E-5 76-77 0.5566010257009364 1.97341260663335E-5 5.9202378199000505E-5 78-79 0.6041208012686675 1.97341260663335E-5 5.9202378199000505E-5 80-81 0.6577482888539288 1.97341260663335E-5 5.9202378199000505E-5 82-83 0.7185984665794681 1.97341260663335E-5 5.9202378199000505E-5 84-85 0.7836026778419707 1.97341260663335E-5 5.9202378199000505E-5 86-87 0.855336226093093 1.97341260663335E-5 5.9202378199000505E-5 88-89 0.9347167481949195 1.97341260663335E-5 5.9202378199000505E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5070 0.0 81.73352 9 ATCGGGA 8400 0.0 75.94565 2 AGAGCGG 5735 0.0 74.07494 8 TCGGGAG 8260 0.0 72.97135 3 CGGGAGA 7855 0.0 72.93024 4 GATCGGG 9155 0.0 71.91597 1 ATTCGGA 1050 0.0 66.85476 2 GAGAGCG 7490 0.0 63.87135 7 ATCCGGA 1385 0.0 63.012672 2 GAGCGTC 14100 0.0 60.96428 9 GATCCGG 1480 0.0 60.895626 1 AGAGCGT 14295 0.0 60.530666 8 TCGGAGA 1765 0.0 60.453953 4 AATCGGA 1255 0.0 59.7136 2 AAGCGGC 1360 0.0 58.220284 9 CGGAAAG 2355 0.0 57.992996 5 GTTCGGA 1005 0.0 56.16177 2 CGGAGAG 3285 0.0 56.010612 5 CGGAAGA 15870 0.0 55.58056 4 AAGAGCG 15015 0.0 55.386124 7 >>END_MODULE