FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5067364
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT144580.28531599466704977Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC50700.081.733529
ATCGGGA84000.075.945652
AGAGCGG57350.074.074948
TCGGGAG82600.072.971353
CGGGAGA78550.072.930244
GATCGGG91550.071.915971
ATTCGGA10500.066.854762
GAGAGCG74900.063.871357
ATCCGGA13850.063.0126722
GAGCGTC141000.060.964289
GATCCGG14800.060.8956261
AGAGCGT142950.060.5306668
TCGGAGA17650.060.4539534
AATCGGA12550.059.71362
AAGCGGC13600.058.2202849
CGGAAAG23550.057.9929965
GTTCGGA10050.056.161772
CGGAGAG32850.056.0106125
CGGAAGA158700.055.580564
AAGAGCG150150.055.3861247
Produced by FastQC (version 0.11.2)