##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3372912 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0300947667772 33.0 33.0 33.0 33.0 33.0 2 31.92162944067322 33.0 33.0 33.0 33.0 33.0 3 31.874896528578272 33.0 33.0 33.0 33.0 33.0 4 35.57710696276689 37.0 37.0 37.0 33.0 37.0 5 35.61753819844692 37.0 37.0 37.0 33.0 37.0 6 35.68701851693729 37.0 37.0 37.0 33.0 37.0 7 35.49644876593282 37.0 37.0 37.0 33.0 37.0 8 35.54538274345729 37.0 37.0 37.0 33.0 37.0 9 35.39698604647853 37.0 37.0 37.0 33.0 37.0 10-11 35.33870495287158 37.0 37.0 37.0 33.0 37.0 12-13 35.31057866318481 37.0 37.0 37.0 33.0 37.0 14-15 37.028992455184124 40.0 37.0 40.0 33.0 40.0 16-17 37.0032746184899 40.0 37.0 40.0 33.0 40.0 18-19 36.995777980569905 40.0 37.0 40.0 33.0 40.0 20-21 36.99687554848748 40.0 37.0 40.0 33.0 40.0 22-23 37.117677543914574 40.0 37.0 40.0 33.0 40.0 24-25 36.78643543027509 40.0 37.0 40.0 33.0 40.0 26-27 36.726571579691374 40.0 37.0 40.0 33.0 40.0 28-29 36.57965683658512 40.0 37.0 40.0 33.0 40.0 30-31 36.5723005818118 40.0 37.0 40.0 33.0 40.0 32-33 36.46246952188494 40.0 37.0 40.0 33.0 40.0 34-35 36.51256110446997 40.0 37.0 40.0 33.0 40.0 36-37 36.332094344590075 40.0 37.0 40.0 33.0 40.0 38-39 36.2577527963967 40.0 37.0 40.0 33.0 40.0 40-41 36.272726504575274 40.0 37.0 40.0 33.0 40.0 42-43 36.08563564658669 40.0 37.0 40.0 27.0 40.0 44-45 35.86683465207512 37.0 37.0 40.0 27.0 40.0 46-47 35.78896291987458 37.0 37.0 40.0 27.0 40.0 48-49 35.68052768646203 37.0 37.0 40.0 27.0 40.0 50-51 34.563211996043776 37.0 35.0 38.5 24.5 38.5 52-53 34.99289471530832 37.0 35.0 38.5 27.0 40.0 54-55 35.71026964237431 37.0 37.0 40.0 27.0 40.0 56-57 35.756842751901026 37.0 37.0 40.0 27.0 40.0 58-59 35.53255288012258 37.0 37.0 40.0 27.0 40.0 60-61 35.372131262244615 37.0 37.0 40.0 27.0 40.0 62-63 35.080386324932284 37.0 35.0 40.0 27.0 40.0 64-65 34.93379489295896 37.0 33.0 40.0 27.0 40.0 66-67 34.75015757897034 37.0 33.0 40.0 27.0 40.0 68-69 34.599949983871504 37.0 33.0 40.0 27.0 40.0 70-71 34.25312252439436 37.0 33.0 38.5 27.0 40.0 72-73 34.10102442637104 37.0 33.0 37.0 27.0 40.0 74-75 33.91867190724217 37.0 33.0 37.0 27.0 40.0 76-77 33.615705064347964 37.0 33.0 37.0 27.0 40.0 78-79 33.24236060709559 37.0 33.0 37.0 22.0 37.0 80-81 32.84955018097122 37.0 33.0 37.0 22.0 37.0 82-83 32.519797877916766 37.0 33.0 37.0 22.0 37.0 84-85 32.29000015416945 37.0 33.0 37.0 22.0 37.0 86-87 32.08090145844303 37.0 33.0 37.0 22.0 37.0 88-89 31.863453300886594 35.0 33.0 37.0 18.5 37.0 90-91 31.672016643185472 33.0 33.0 37.0 15.0 37.0 92-93 31.515173090789204 33.0 33.0 37.0 15.0 37.0 94-95 31.33780602636535 33.0 33.0 37.0 15.0 37.0 96-97 31.147443069964467 33.0 33.0 37.0 15.0 37.0 98-99 30.97850018025967 33.0 33.0 37.0 15.0 37.0 100-101 29.645497718292084 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16721069317069492 2306 2 -0.002224154909384879 2306 3 -0.038622904488352816 2306 4 0.024451230642632993 2306 5 0.021233801702493338 2306 6 0.15124455077999954 2306 7 0.22160869852104526 2306 8 0.048169445326252 2306 9 0.065302778423451 2306 10-11 0.1794602324456207 2306 12-13 0.14732965845715995 2306 14-15 0.44610636613870724 2306 16-17 0.4331711657570452 2306 18-19 0.4249599557327315 2306 20-21 0.2968501196065958 2306 22-23 0.36317825327861186 2306 24-25 0.2988718917139437 2306 26-27 0.2506243296696766 2306 28-29 0.05536790241820455 2306 30-31 0.12409104761636769 2306 32-33 0.13716441305751914 2306 34-35 0.15959997190435615 2306 36-37 0.1265446968604067 2306 38-39 0.15080017340370233 2306 40-41 0.13835577541097877 2306 42-43 0.28724625851045715 2306 44-45 0.18844897507737812 2306 46-47 -0.004795121726715479 2306 48-49 0.09033855236851451 2306 50-51 0.22300396175299397 2306 52-53 0.14223891763824525 2306 54-55 0.2524831790090971 2306 56-57 0.27110047496611145 2306 58-59 0.31991574388311506 2306 60-61 0.27335543202670465 2306 62-63 0.29759119981336823 2306 64-65 0.20993836879694783 2306 66-67 0.33431677788816927 2306 68-69 0.30333043077379784 2306 70-71 0.25135189745123654 2306 72-73 0.029198557635162103 2306 74-75 0.17050044100860617 2306 76-77 0.2672046042359355 2306 78-79 0.38944241533236124 2306 80-81 0.36046465393526006 2306 82-83 0.4143083160393175 2306 84-85 0.35333333930510946 2306 86-87 0.43696028076598026 2306 88-89 0.3821471838359223 2306 90-91 0.42888774666662854 2306 92-93 0.45114560134659953 2306 94-95 0.5084919933522336 2306 96-97 0.5630075431357362 2306 98-99 0.3018072287648579 2306 100-101 0.3717097671988796 2307 1 0.1103157728453894 2307 2 0.07240958466048042 2307 3 0.09198267075791122 2307 4 0.10904199424574301 2307 5 0.012883751088118345 2307 6 0.1546402071942765 2307 7 0.20787277605751342 2307 8 0.12221907120274977 2307 9 0.1493669146355927 2307 10-11 0.21934779716727348 2307 12-13 0.21027484691972376 2307 14-15 0.4277292507088575 2307 16-17 0.3931428587088277 2307 18-19 0.3110653842898188 2307 20-21 0.3115021887170215 2307 22-23 0.28364364123846286 2307 24-25 0.3150800555617934 2307 26-27 0.24815539209583903 2307 28-29 0.2557235245785776 2307 30-31 0.2335294394800087 2307 32-33 0.3097425878211624 2307 34-35 0.19498972903463851 2307 36-37 0.2343113606197207 2307 38-39 0.3023826325487775 2307 40-41 0.2876103696898298 2307 42-43 0.2687227842762141 2307 44-45 0.15073597841555397 2307 46-47 0.0602909359287338 2307 48-49 0.09547862071774205 2307 50-51 -0.04254145771199802 2307 52-53 -0.009794181256694401 2307 54-55 0.10547748852592065 2307 56-57 0.10161070224184954 2307 58-59 0.20434812281168035 2307 60-61 0.07178698103806624 2307 62-63 -0.042429706699365966 2307 64-65 -0.06554160878999937 2307 66-67 -0.022355663839228157 2307 68-69 0.052998397820275045 2307 70-71 -0.017826722401757422 2307 72-73 0.09274404846246398 2307 74-75 0.19638876957123585 2307 76-77 0.057692140941277614 2307 78-79 0.09502697834395946 2307 80-81 0.19558171454828965 2307 82-83 -0.015989211917521118 2307 84-85 -0.04583073056652154 2307 86-87 -0.03774706770144576 2307 88-89 0.03287527759013997 2307 90-91 0.0053266882843381325 2307 92-93 7.856842325928426E-4 2307 94-95 -0.26568562214237446 2307 96-97 -0.12271087706138317 2307 98-99 -0.11825971965076576 2307 100-101 -0.25774674276144793 2201 1 0.08777068601360583 2201 2 -0.005872698556885325 2201 3 0.0377778231494581 2201 4 0.08306401367502048 2201 5 0.07582858928427072 2201 6 0.13081577131290345 2201 7 0.12152536477353237 2201 8 0.077925640270891 2201 9 0.050480388650136376 2201 10-11 0.0893392576892893 2201 12-13 0.11706381054870718 2201 14-15 0.20902268282019776 2201 16-17 0.18226493196198135 2201 18-19 0.28584374905841514 2201 20-21 0.15454387056957586 2201 22-23 0.20893461058323481 2201 24-25 0.16600571610339898 2201 26-27 0.16209361298287916 2201 28-29 0.05492224082611585 2201 30-31 0.121809517058729 2201 32-33 0.13245121037427054 2201 34-35 0.0476916953556028 2201 36-37 0.05422057165766603 2201 38-39 0.08212956205208144 2201 40-41 0.00242256997755419 2201 42-43 0.006174823430434628 2201 44-45 -0.05107052220309072 2201 46-47 -0.08468596777134962 2201 48-49 -0.12120532541749895 2201 50-51 -0.21585197192460726 2201 52-53 -0.037811886126512206 2201 54-55 0.05143098690079029 2201 56-57 0.25199977532711415 2201 58-59 0.2929173906772533 2201 60-61 0.07400864103868088 2201 62-63 0.248942432506702 2201 64-65 0.1388920001105589 2201 66-67 0.06797329871248792 2201 68-69 0.10317225404330088 2201 70-71 0.20294915522421064 2201 72-73 0.15431183685954863 2201 74-75 0.31832775791044554 2201 76-77 0.22702837738258808 2201 78-79 0.2162140444160059 2201 80-81 0.11973801844833787 2201 82-83 0.09594026583363302 2201 84-85 0.15143062110074368 2201 86-87 0.2573814392541749 2201 88-89 0.2638548878172209 2201 90-91 0.27076527422226704 2201 92-93 0.17008162157201312 2201 94-95 0.059481933660087805 2201 96-97 0.11032015403340623 2201 98-99 0.07630660500430508 2201 100-101 -0.049635103633811184 1101 1 0.1516540667441646 1101 2 0.013408270330597105 1101 3 0.03855108795690043 1101 4 0.023907678529575094 1101 5 0.032073228396399145 1101 6 0.22438431829364447 1101 7 0.26571261123223167 1101 8 0.13234599136676195 1101 9 0.09045600592121872 1101 10-11 0.0916628202878087 1101 12-13 0.007935149590267088 1101 14-15 0.2637155694499782 1101 16-17 0.20772448295662116 1101 18-19 0.09394604645409288 1101 20-21 0.16330566890449205 1101 22-23 0.27701346115980385 1101 24-25 0.03572698323413448 1101 26-27 0.020774737294487977 1101 28-29 -0.002437725859699924 1101 30-31 0.17288162452781108 1101 32-33 0.06975305250416852 1101 34-35 -0.020074046068991436 1101 36-37 -0.11978171168184559 1101 38-39 -0.06337006130556233 1101 40-41 -0.04214662933583213 1101 42-43 -0.021005031443955602 1101 44-45 -0.20109546227156727 1101 46-47 -0.17041916196842521 1101 48-49 -0.3694368239352883 1101 50-51 -0.2756460927985884 1101 52-53 -0.16876705043827656 1101 54-55 0.162644500877569 1101 56-57 -0.03278609937523669 1101 58-59 -0.24149788827820373 1101 60-61 -0.09558969591619615 1101 62-63 -0.04555140328284324 1101 64-65 -0.06997064513510765 1101 66-67 -0.17052403674878036 1101 68-69 -0.06594165319086187 1101 70-71 -0.03353185544620629 1101 72-73 -0.05249881736945383 1101 74-75 -0.02653572720035413 1101 76-77 -0.13357258888294865 1101 78-79 -0.05475620662164005 1101 80-81 0.015048970100359327 1101 82-83 -0.07035793746489816 1101 84-85 -0.04809413301261145 1101 86-87 -0.0964984151645254 1101 88-89 -0.11478188418428559 1101 90-91 -0.21262372880243774 1101 92-93 -0.08128499953769364 1101 94-95 -0.11492801376421369 1101 96-97 0.0442379110466824 1101 98-99 -0.20686859345589426 1101 100-101 -0.28575971494205277 2304 1 0.10129088577495793 2304 2 0.04619447829562162 2304 3 0.07230927351526262 2304 4 0.08177688643278458 2304 5 0.05608581668775514 2304 6 0.2167682768292778 2304 7 0.1927750120059315 2304 8 0.23322837675721075 2304 9 0.1825123465061722 2304 10-11 0.21960424185394345 2304 12-13 0.11544446287581422 2304 14-15 0.13244100495023758 2304 16-17 0.24764551662372014 2304 18-19 0.2853713292955007 2304 20-21 0.20452183806708746 2304 22-23 0.271803654999772 2304 24-25 0.14868115506180857 2304 26-27 0.24637791973472645 2304 28-29 0.09444219633201101 2304 30-31 0.08036464700098378 2304 32-33 0.11261411886263772 2304 34-35 0.04214816919798636 2304 36-37 -0.012466449630522902 2304 38-39 -0.09534727906468987 2304 40-41 0.22323783858428925 2304 42-43 0.17139754975044497 2304 44-45 0.07708817122825451 2304 46-47 -0.07813794104188077 2304 48-49 -0.042871444709881246 2304 50-51 -0.0692133726306352 2304 52-53 0.02433990543776332 2304 54-55 0.23788974486178205 2304 56-57 0.26492691293370996 2304 58-59 0.132247057978212 2304 60-61 0.15065592130365957 2304 62-63 0.16910126456951957 2304 64-65 0.17095366471150442 2304 66-67 0.07841059850140653 2304 68-69 0.1700338024578656 2304 70-71 0.12507036534497473 2304 72-73 0.07905673018757398 2304 74-75 0.19204702587023093 2304 76-77 0.37223032548490664 2304 78-79 0.25652411681794973 2304 80-81 0.21001467076224145 2304 82-83 0.3371486371943533 2304 84-85 0.20681125527373467 2304 86-87 0.22415106179697375 2304 88-89 0.2895940493938589 2304 90-91 0.04867979471414685 2304 92-93 0.1853201914893141 2304 94-95 0.14173925018380373 2304 96-97 0.2550121363479896 2304 98-99 0.11963810092045435 2304 100-101 -0.013895578974736367 2202 1 0.1979788889556957 2202 2 0.19245195305995466 2202 3 0.19969124098614444 2202 4 0.11888164084352582 2202 5 0.15304368371054977 2202 6 0.2303790841280744 2202 7 0.2931471830591619 2202 8 0.17171036493889602 2202 9 0.1527709406236113 2202 10-11 0.29321791680239073 2202 12-13 0.23726201247610845 2202 14-15 0.3166117866479894 2202 16-17 0.3448167949651406 2202 18-19 0.2703282903072477 2202 20-21 0.2720424501648395 2202 22-23 0.2617193641092683 2202 24-25 0.2718167152336193 2202 26-27 0.30074544020828853 2202 28-29 0.3732777643309859 2202 30-31 0.3318705440385088 2202 32-33 0.26078155912513523 2202 34-35 0.2714800691682697 2202 36-37 0.18859520414441988 2202 38-39 0.1151836447964385 2202 40-41 0.22063575822221537 2202 42-43 0.20792233240823066 2202 44-45 0.24177985386670997 2202 46-47 0.11687698943839564 2202 48-49 0.1939250027293582 2202 50-51 0.1673722956321697 2202 52-53 0.13050708387137178 2202 54-55 0.2253342187831393 2202 56-57 0.3633882872859786 2202 58-59 0.3030413622995667 2202 60-61 0.306104559551045 2202 62-63 0.3987616974460906 2202 64-65 0.3189619296380002 2202 66-67 0.3284122262735565 2202 68-69 0.2863839078474584 2202 70-71 0.33671613996018124 2202 72-73 0.3749933953049691 2202 74-75 0.286575079311028 2202 76-77 0.29617222163858514 2202 78-79 0.3200764282197639 2202 80-81 0.2232738100101912 2202 82-83 0.1911697539014554 2202 84-85 0.2330628562049597 2202 86-87 0.2707469746342781 2202 88-89 0.4660695555790433 2202 90-91 0.29398187112800755 2202 92-93 0.1708899567645723 2202 94-95 0.24239499992526703 2202 96-97 0.17310375013515156 2202 98-99 0.25953361872862857 2202 100-101 0.45033340077231543 1102 1 0.10299377339612192 1102 2 0.0021023958287678113 1102 3 0.10089931246357864 1102 4 0.1491614763542728 1102 5 0.12699177212276425 1102 6 0.19788728675722922 1102 7 0.23936242948435904 1102 8 0.20682080492706945 1102 9 0.14543977023127752 1102 10-11 0.3027257264281147 1102 12-13 0.3076652776670983 1102 14-15 0.35401273899117314 1102 16-17 0.3660483286974312 1102 18-19 0.3278564266929891 1102 20-21 0.3701415407358084 1102 22-23 0.2960828842555898 1102 24-25 0.1565737281866717 1102 26-27 0.2384713983612059 1102 28-29 0.21499337238961402 1102 30-31 -0.0037190665166733083 1102 32-33 0.20556451381831664 1102 34-35 0.19595056856370974 1102 36-37 0.26356732248312653 1102 38-39 0.23090132277354058 1102 40-41 0.2643717577885525 1102 42-43 0.13064997701265924 1102 44-45 0.08167167251896501 1102 46-47 0.05600336739728107 1102 48-49 0.16788952902469845 1102 50-51 0.21706081656900977 1102 52-53 0.08881258155275162 1102 54-55 0.24699928599776655 1102 56-57 0.3381831148990173 1102 58-59 0.34802626454310115 1102 60-61 0.38459394569775185 1102 62-63 0.3432092440355632 1102 64-65 0.3761719114233273 1102 66-67 0.4404197736460276 1102 68-69 0.27203093806154044 1102 70-71 0.29670263078081405 1102 72-73 0.37757935037609514 1102 74-75 0.2512752272268841 1102 76-77 0.3504314151834862 1102 78-79 0.25603719613743436 1102 80-81 0.3325521041243249 1102 82-83 0.39672240808001646 1102 84-85 0.37325604023045145 1102 86-87 0.3387540105501756 1102 88-89 0.42784495235942543 1102 90-91 0.4465034674949031 1102 92-93 0.45531270348213937 1102 94-95 0.4951847345550924 1102 96-97 0.3925476076119274 1102 98-99 0.31622091347467673 1102 100-101 0.26195519745552787 2305 1 0.11200287406663989 2305 2 0.01099141459277675 2305 3 0.0617528475565976 2305 4 -0.012409379542233978 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-1.5564330485583753 1213 100-101 -1.3410865893552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1395.0 3 246.0 4 222.0 5 351.0 6 606.0 7 1662.0 8 6336.0 9 14211.0 10 21715.0 11 19153.0 12 13267.0 13 10452.0 14 8738.0 15 8161.0 16 8220.0 17 8880.0 18 9793.0 19 11094.0 20 12211.0 21 14034.0 22 15710.0 23 17731.0 24 20389.0 25 24093.0 26 29141.0 27 36459.0 28 44827.0 29 51979.0 30 58623.0 31 72883.0 32 96212.0 33 133855.0 34 196846.0 35 311839.0 36 544560.0 37 973828.0 38 569600.0 39 3590.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.581223339492794 28.97080974277892 16.16432065589489 18.283646261833397 2 31.847161760140917 26.78560554414388 27.068486395846055 14.29874629986915 3 35.191628628792415 23.35035992821926 23.690495881634828 17.767515561353502 4 30.328757311261363 23.604594394264076 26.929585010191815 19.13706328428275 5 33.55208430163577 21.40660921545748 26.898261347069198 18.14304513583755 6 31.541967258683957 21.82488111939616 28.376084417998815 18.25706720392107 7 33.78427925792241 22.89314546954616 23.059993878266567 20.262581394264867 8 29.39869007493555 28.426828381412893 22.639411439062364 19.535070104589188 9 30.038035792945657 24.142191056394132 25.037222478815245 20.782550671844966 10-11 25.995456793464832 29.766045191980822 27.811300087412732 16.427197927141616 12-13 26.456209971362494 28.117455488355503 27.286624899740524 18.13970964054148 14-15 24.855430246680235 28.13710390900465 28.21199536759373 18.795470476721388 16-17 25.505443858006753 27.998603788884807 28.285622527913834 18.210329825194606 18-19 24.967972996397776 28.048264308561034 28.65495192684427 18.328810768196917 20-21 24.335446362904072 29.102184044301794 28.008870806415903 18.55349878637823 22-23 25.50856321320537 28.597487427640672 27.382604555966587 18.51134480318737 24-25 23.272885466478495 30.20920893430215 27.778282494613098 18.739623104606252 26-27 23.32502900912157 30.039337603123094 27.73054909214796 18.90508429560737 28-29 23.513737492866994 29.8799065185482 27.645612304402327 18.960743684182482 30-31 24.01504189658696 28.49103171506605 28.412537391078164 19.081388997268824 32-33 22.723208444840918 29.542060660124886 28.577044305679454 19.157686589354743 34-35 24.327907301127684 28.549199359618555 27.960879391358063 19.162013947895694 36-37 22.800829733628266 29.092907032307693 28.703820717589945 19.402442516474096 38-39 22.798905876947302 29.937643791815553 27.919906313222103 19.34354401801504 40-41 24.205680526003828 28.40569406133588 27.8683860008969 19.52023941176339 42-43 23.79249653885117 28.36073682293322 28.228376896786934 19.618389741428672 44-45 22.543353976738466 29.19897063587419 28.569004193430263 19.688671193957084 46-47 23.168373511810454 29.235476790446786 27.78558823450651 19.810561463236255 48-49 21.743784783633547 28.941887278824403 28.77690885172486 20.537419085817195 50-51 21.419649569792114 28.319446372465745 29.395170667453446 20.865733390288693 52-53 23.639980153785846 28.328432528450826 27.890967815361773 20.140619502401556 54-55 23.34844601008378 28.27797885375488 28.563658122515907 19.809917013645435 56-57 23.545231160860684 28.177894683914584 28.35481587729735 19.92205827792738 58-59 23.00392812284874 28.47291100558636 27.9106753670046 20.6124855045603 60-61 21.757297723318512 28.306080568649904 27.895474891073206 22.041146816958378 62-63 22.917172312048635 28.390173823036015 28.218254393166053 20.474399471749294 64-65 21.662617009511862 29.10019979101806 28.967645276802916 20.269537922667162 66-67 21.410068621023235 28.962441680459488 28.210222235972076 21.4172674625452 68-69 21.245444942303966 28.41255486406472 30.086588295061407 20.255411898569907 70-71 21.193834940354204 29.904209243723223 28.63691261654879 20.265043199373785 72-73 21.155321246611734 30.824961197826333 27.814337750712443 20.20537980484949 74-75 21.00512635675375 30.939695273509777 27.94701575139876 20.108162618337712 76-77 21.14661181830698 30.870415397822583 27.788228199546296 20.194744584324148 78-79 21.095216364872677 30.97935060874018 27.791499629086243 20.1339333973009 80-81 21.13014392758875 30.743686596623405 27.807898076063157 20.318271399724686 82-83 21.260365118730597 30.711006202820833 27.67397268057622 20.35465599787235 84-85 21.25301294422567 30.48033823808302 27.828188738040254 20.43846007965105 86-87 21.509643813750895 30.088005814800045 27.7898241891577 20.61252618229136 88-89 21.528674926759177 30.139416022020963 27.768443709619 20.563465341600864 90-91 21.59203710749316 29.90222486110421 27.798537820080536 20.707200211322093 92-93 21.697670441448743 29.906118716028818 27.681624519477428 20.714586323045005 94-95 21.60470962259227 29.842950621974424 27.81121955462899 20.741120200804318 96-97 21.73231579916125 29.62887081985503 27.781082565050824 20.8577308159329 98-99 21.73627447962373 29.755646558602162 27.72315760842328 20.78492135335083 100-101 21.920812958798823 29.615084888936476 27.644584835397556 20.819517316867138 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 630.0 1 585.5 2 395.0 3 212.5 4 186.0 5 228.0 6 310.0 7 415.5 8 570.5 9 802.5 10 997.0 11 1149.0 12 1288.5 13 1246.5 14 1189.5 15 1146.0 16 1009.5 17 972.5 18 1087.0 19 1369.5 20 1861.5 21 2662.5 22 3862.5 23 5527.0 24 7918.5 25 10940.0 26 14859.0 27 19713.0 28 25126.5 29 31637.5 30 39092.0 31 47447.0 32 57472.5 33 68264.5 34 79737.0 35 92192.5 36 105476.0 37 119123.0 38 130900.5 39 140730.5 40 149816.0 41 157093.0 42 160996.0 43 161715.0 44 160729.0 45 159146.5 46 155881.0 47 151092.5 48 144697.0 49 136320.5 50 126775.0 51 115520.5 52 102801.0 53 88643.0 54 74303.0 55 61318.0 56 50375.0 57 41372.5 58 33492.0 59 26871.0 60 21403.0 61 16897.5 62 13104.5 63 9976.0 64 7592.5 65 5807.5 66 4424.5 67 3348.0 68 2543.5 69 1894.0 70 1403.5 71 1132.0 72 906.5 73 688.0 74 553.0 75 441.0 76 349.0 77 283.0 78 225.0 79 181.0 80 140.5 81 107.5 82 99.5 83 82.5 84 56.5 85 51.0 86 48.0 87 40.0 88 32.5 89 31.0 90 29.5 91 19.0 92 12.0 93 9.5 94 6.0 95 5.0 96 4.0 97 2.0 98 1.0 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1860410233056777 2 0.19202991361766925 3 0.1611367269587822 4 0.1631824370158486 5 0.17092055766649114 6 0.16804470439786154 7 0.13593595089347127 8 0.11909590288747528 9 0.11799892792933821 10-11 0.09681545204855627 12-13 0.14161353750112662 14-15 0.11967403833838534 16-17 0.11248440516681134 18-19 0.11503413074518398 20-21 0.1084522809963616 22-23 0.10720706617901683 24-25 0.46529527008116434 26-27 0.3144908613091596 28-29 0.40110741104422526 30-31 0.26607572329192103 32-33 0.2938706968933669 34-35 0.31251927118169703 36-37 0.29366316109047613 38-39 0.3409072042199737 40-41 0.33619317669716847 42-43 0.3781895288107131 44-45 0.3327984839213119 46-47 0.35675404516927806 48-49 0.3518176578576613 50-51 0.3672049552434217 52-53 0.35783619614149437 54-55 0.334280882513389 56-57 0.3501721954204557 58-59 0.34124815589615143 60-61 0.3131418785903694 62-63 0.3223327498612475 64-65 0.32313324510096914 66-67 0.3338361629357659 68-69 0.3217546144103374 70-71 0.3106810969275214 72-73 0.2970875018381743 74-75 0.3050776302494699 76-77 0.2947897840204547 78-79 0.3044994947985598 80-81 0.3131270546044486 82-83 0.33969163737447045 84-85 0.3372308557116225 86-87 0.33044147015990927 88-89 0.3430122102207232 90-91 0.3326205960902627 92-93 0.31684787507056217 94-95 0.30936176218057276 96-97 0.29486390395005857 98-99 0.31881946519802473 100-101 0.3223327498612475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3372912.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.19670302497448 #Duplication Level Percentage of deduplicated Percentage of total 1 97.91581709045218 93.21262961007497 2 1.5340016533823482 2.920637996737185 3 0.23527591646261628 0.6719247464526116 4 0.1145064165719877 0.4360253333143012 5 0.06026930718519505 0.2868719668814987 6 0.04372537108863588 0.2497506699709005 7 0.00938086548893247 0.06251192262469954 8 0.014792427421672326 0.11265522562235436 9 0.004388149799139195 0.03759636539319798 >10 0.06474895696292349 1.133305699059315 >50 0.002368078433463087 0.147523486190371 >100 6.947224929802349E-4 0.08753046846088873 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.104425791071751E-5 0.6410365092176458 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 21691 0.6430941572148933 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002816557324946515 0.0 0.0 2 0.004150716057815917 0.0 0.0 3 0.007026569326445516 0.0 0.0 4 0.009487350989293524 0.0 0.0 5 0.01245214817344775 0.0 0.0 6 0.02196914713458282 0.0 0.0 7 0.031219314349144003 0.0 0.0 8 0.039728282267666636 2.9647971841542265E-5 0.0 9 0.04393829426916564 2.9647971841542265E-5 5.929594368308453E-5 10-11 0.05421131651226003 2.9647971841542265E-5 5.929594368308453E-5 12-13 0.06690064846044011 2.9647971841542265E-5 5.929594368308453E-5 14-15 0.07828546964759235 2.9647971841542265E-5 7.411992960385566E-5 16-17 0.09147881711707866 2.9647971841542265E-5 1.6306384512848246E-4 18-19 0.10729601009454146 2.9647971841542265E-5 1.927118169700247E-4 20-21 0.12711567927061246 2.9647971841542265E-5 2.3718377473233812E-4 22-23 0.14792855550337514 2.9647971841542265E-5 2.3718377473233812E-4 24-25 0.1679705844682577 2.9647971841542265E-5 2.3718377473233812E-4 26-27 0.18890205258838655 2.9647971841542265E-5 2.3718377473233812E-4 28-29 0.21140486321611712 4.4471957762313396E-5 2.5200776065310924E-4 30-31 0.24167544246633177 5.929594368308453E-5 3.261276902569649E-4 32-33 0.2781869197891911 5.929594368308453E-5 3.5577566209850717E-4 34-35 0.3175446024088384 5.929594368308453E-5 3.5577566209850717E-4 36-37 0.36441804589031673 5.929594368308453E-5 3.854236339400494E-4 38-39 0.4193853856845361 5.929594368308453E-5 4.0024761986082055E-4 40-41 0.4828616933972781 5.929594368308453E-5 4.4471957762313394E-4 42-43 0.5506666049988852 5.929594368308453E-5 4.4471957762313394E-4 44-45 0.6237785035601284 5.929594368308453E-5 4.891915353854474E-4 46-47 0.7045099308846481 5.929594368308453E-5 5.188395072269897E-4 48-49 0.7899109137742105 5.929594368308453E-5 5.484874790685319E-4 50-51 0.8798035643977666 5.929594368308453E-5 5.929594368308453E-4 52-53 0.9741285868116334 5.929594368308453E-5 5.929594368308453E-4 54-55 1.072797037100286 5.929594368308453E-5 5.929594368308453E-4 56-57 1.1737039092629753 5.929594368308453E-5 5.929594368308453E-4 58-59 1.2817559426394758 5.929594368308453E-5 6.226074086723876E-4 60-61 1.3948481312290388 5.929594368308453E-5 6.226074086723876E-4 62-63 1.5086815191146403 5.929594368308453E-5 6.226074086723876E-4 64-65 1.6262357274663555 5.929594368308453E-5 6.374313945931586E-4 66-67 1.748933858932578 5.929594368308453E-5 6.670793664347009E-4 68-69 1.8763904898793684 5.929594368308453E-5 6.81903352355472E-4 70-71 2.0103548506453772 5.929594368308453E-5 6.81903352355472E-4 72-73 2.150204333821932 5.929594368308453E-5 6.967273382762432E-4 74-75 2.2966949626909923 5.929594368308453E-5 7.263753101177854E-4 76-77 2.4441343266589817 5.929594368308453E-5 7.708472678800988E-4 78-79 2.5972809252064684 5.929594368308453E-5 8.004952397216411E-4 80-81 2.7567721897280455 5.929594368308453E-5 8.004952397216411E-4 82-83 2.9216593851247823 5.929594368308453E-5 8.004952397216411E-4 84-85 3.092550294819432 5.929594368308453E-5 8.153192256424122E-4 86-87 3.272320772080623 5.929594368308453E-5 8.449671974839546E-4 88-89 3.463298182697918 5.929594368308453E-5 9.339111130085814E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1515 0.0 77.93613 2 GATCGGG 1745 0.0 70.114624 1 GAGCGGC 1115 0.0 67.17164 9 TCGGGAG 1900 0.0 57.39423 3 GATCCGG 330 0.0 51.73362 1 CGGGAGA 2120 0.0 48.978146 4 GAGAGCG 1960 0.0 48.61203 7 AGAGCGG 1715 0.0 45.606167 8 TCGGAGA 590 0.0 41.787403 4 GGATCGG 645 0.0 40.437782 1 AGCGGCG 1085 0.0 39.975613 10-11 GCGTCGG 530 0.0 39.35325 10-11 CGCCGGA 750 0.0 39.26704 58-59 TCGGGAA 680 0.0 39.045692 3 CGTCGGG 545 0.0 38.705017 12-13 AAGCGGC 420 0.0 38.373672 9 GGCGCCG 1400 0.0 37.83578 56-57 TTCGGAG 465 0.0 37.726177 3 GTTCGGA 350 0.0 36.580345 2 GGTTCGG 480 0.0 36.554832 1 >>END_MODULE