FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372912
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT216910.6430941572148933Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA15150.077.936132
GATCGGG17450.070.1146241
GAGCGGC11150.067.171649
TCGGGAG19000.057.394233
GATCCGG3300.051.733621
CGGGAGA21200.048.9781464
GAGAGCG19600.048.612037
AGAGCGG17150.045.6061678
TCGGAGA5900.041.7874034
GGATCGG6450.040.4377821
AGCGGCG10850.039.97561310-11
GCGTCGG5300.039.3532510-11
CGCCGGA7500.039.2670458-59
TCGGGAA6800.039.0456923
CGTCGGG5450.038.70501712-13
AAGCGGC4200.038.3736729
GGCGCCG14000.037.8357856-57
TTCGGAG4650.037.7261773
GTTCGGA3500.036.5803452
GGTTCGG4800.036.5548321
Produced by FastQC (version 0.11.2)