FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34211-1.34200000035897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34211-1.34200000035897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662890
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT78660.2147484636448269Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA11350.066.4626542
GATCGGG14050.055.380371
GAGCGGC8350.052.2516029
TCGGGAG15350.045.120453
CGGTCCG554.999653E-543.1316071
CGCCGGA2650.038.5332558-59
AGCGGCG7050.036.66111410-11
CGCCGTA10300.036.19724358-59
TCGCCGT9700.035.50111856-57
CGGGAGA19600.035.3366784
CGACGGG2150.035.3077351
GTCGCCG10950.035.1355456-57
AGAGCGG13100.034.753478
AAGCGGC3300.034.490199
TTGTGTT307500.033.496781
GCCGTAT11400.033.32945358-59
GGCGCCG6700.033.31952756-57
GTGTTTG694150.031.9328611
TGTTTGA705550.031.362252
TGGTCGC11400.031.0412454-55
Produced by FastQC (version 0.11.2)