FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711023
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT109140.40257865757686306Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC25500.088.118179
CGGGAGA36400.084.5455864
AGAGCGG27450.082.721898
ATCGGGA41950.082.0790942
TCGGGAG39000.080.125083
GAGAGCG34700.078.2817467
GTTCGGA4200.075.6577452
GATCCGG6050.073.6899641
GATCGGG48550.073.657681
AAGCGGC7800.072.93139
ATTCGGA5150.071.831562
CGGAAAG12350.067.960095
TCGGGAA9350.067.450893
TCGGAGA6750.066.737124
ATCCGGA6650.066.326822
TTCGGAG5050.065.728593
GGACCGG5400.064.115791
ACCGGAA4200.063.224643
GGATCGG12400.061.962541
AAAGCGG9900.061.7705968
Produced by FastQC (version 0.11.2)