##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2711023 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.348376240260595 33.0 33.0 33.0 27.0 33.0 2 30.90827374020803 33.0 33.0 33.0 27.0 33.0 3 30.79158347236449 33.0 33.0 33.0 27.0 33.0 4 34.2274794422622 37.0 37.0 37.0 27.0 37.0 5 34.32536758264316 37.0 37.0 37.0 27.0 37.0 6 34.8125209561114 37.0 37.0 37.0 27.0 37.0 7 34.22405933110859 37.0 37.0 37.0 27.0 37.0 8 34.50458738269649 37.0 37.0 37.0 27.0 37.0 9 34.003926562039496 37.0 37.0 37.0 27.0 37.0 10-11 33.99833808123354 37.0 37.0 37.0 27.0 37.0 12-13 33.98919798909858 37.0 37.0 37.0 27.0 37.0 14-15 35.57428395111366 40.0 37.0 40.0 27.0 40.0 16-17 35.51475310242665 40.0 37.0 40.0 24.5 40.0 18-19 35.55725052867497 40.0 37.0 40.0 24.5 40.0 20-21 35.56353874533709 40.0 37.0 40.0 27.0 40.0 22-23 36.24108390080055 40.0 37.0 40.0 27.0 40.0 24-25 35.57625128964232 40.0 37.0 40.0 24.5 40.0 26-27 35.46740676858883 40.0 37.0 40.0 24.5 40.0 28-29 35.16888218949083 40.0 37.0 40.0 18.5 40.0 30-31 35.176038897493676 40.0 37.0 40.0 18.5 40.0 32-33 34.99331838940503 40.0 37.0 40.0 22.0 40.0 34-35 35.26573400520763 40.0 37.0 40.0 22.0 40.0 36-37 34.886406349189954 38.5 37.0 40.0 18.5 40.0 38-39 34.83629924939774 37.0 37.0 40.0 22.0 40.0 40-41 35.24396325667469 37.0 37.0 40.0 22.0 40.0 42-43 34.90533831693793 37.0 37.0 40.0 22.0 40.0 44-45 34.47471526431167 37.0 37.0 40.0 15.0 40.0 46-47 34.369665436257826 37.0 37.0 40.0 15.0 40.0 48-49 34.27406941955122 37.0 35.0 40.0 15.0 40.0 50-51 33.25134220550693 37.0 33.0 38.5 15.0 38.5 52-53 33.716921804056994 37.0 33.0 38.5 18.5 40.0 54-55 34.380392383244256 37.0 37.0 40.0 18.5 40.0 56-57 34.618134372153975 37.0 35.0 40.0 22.0 40.0 58-59 34.11690015171395 37.0 33.0 40.0 15.0 40.0 60-61 33.92015578621059 37.0 33.0 40.0 15.0 40.0 62-63 33.53284903890524 37.0 33.0 40.0 15.0 40.0 64-65 33.34078279675237 37.0 33.0 40.0 15.0 40.0 66-67 33.21545483015083 37.0 33.0 37.0 15.0 40.0 68-69 33.267066528022816 37.0 33.0 37.0 18.5 40.0 70-71 32.847791221247476 37.0 33.0 37.0 15.0 40.0 72-73 33.000646988240234 37.0 33.0 37.0 22.0 38.5 74-75 33.043538915014736 37.0 33.0 37.0 22.0 37.0 76-77 32.77030183808843 37.0 33.0 37.0 22.0 37.0 78-79 32.35477253420572 37.0 33.0 37.0 22.0 37.0 80-81 31.903890523983012 37.0 33.0 37.0 18.5 37.0 82-83 31.537453758230747 37.0 33.0 37.0 15.0 37.0 84-85 31.306734026232903 35.0 33.0 37.0 15.0 37.0 86-87 31.117849239936362 33.0 33.0 37.0 15.0 37.0 88-89 30.964049179959005 33.0 33.0 37.0 15.0 37.0 90-91 30.823285527271437 33.0 33.0 37.0 15.0 37.0 92-93 30.693295667355088 33.0 33.0 37.0 15.0 37.0 94-95 30.55283356135304 33.0 30.0 37.0 15.0 37.0 96-97 30.390357071850737 33.0 27.0 37.0 15.0 37.0 98-99 30.25618170705302 33.0 27.0 37.0 15.0 37.0 100-101 29.047165774690956 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.0963549443265137 2306 2 -0.03456182072852698 2306 3 -0.12058439570473212 2306 4 0.09049216367230883 2306 5 -0.0075967880400185095 2306 6 -0.09323915029676755 2306 7 0.3852550472347218 2306 8 -0.17823619692842385 2306 9 0.06377005132014091 2306 10-11 0.08752930214471633 2306 12-13 0.11141559156880021 2306 14-15 0.5668774721651673 2306 16-17 0.44527220218716934 2306 18-19 0.47505621061995384 2306 20-21 0.33408891431553656 2306 22-23 0.2478845900444071 2306 24-25 0.4954333101432482 2306 26-27 0.057223111479345334 2306 28-29 0.23024557826389014 2306 30-31 0.17936482905953 2306 32-33 0.22157492298593695 2306 34-35 -0.05333635209969145 2306 36-37 0.18599432051868803 2306 38-39 0.07704947174889298 2306 40-41 -0.07232598570895021 2306 42-43 0.13641607614663798 2306 44-45 0.37012106384482735 2306 46-47 0.21872961905212662 2306 48-49 0.26931005613622716 2306 50-51 0.20905106774907267 2306 52-53 0.04186338094601183 2306 54-55 0.14801080920505427 2306 56-57 0.0778361580179876 2306 58-59 0.300050105323983 2306 60-61 0.3980053488496438 2306 62-63 0.4338630264338832 2306 64-65 0.29731828375671654 2306 66-67 0.28381854413195384 2306 68-69 0.24710949159042883 2306 70-71 0.3666819914315198 2306 72-73 0.018336327753829096 2306 74-75 0.02076523711407674 2306 76-77 0.2846529838332117 2306 78-79 0.192479557516819 2306 80-81 0.3555143049552605 2306 82-83 0.3201834753637094 2306 84-85 0.3267818124734063 2306 86-87 0.5062181160926364 2306 88-89 0.42356959985351494 2306 90-91 0.4449544430845087 2306 92-93 0.3251349892263917 2306 94-95 0.31831415783978656 2306 96-97 0.27606304289570716 2306 98-99 0.0044509160042984774 2306 100-101 0.22110354531244525 2307 1 -0.11454245313363742 2307 2 -0.24885434889711888 2307 3 -0.35788468079759284 2307 4 -0.17675831647930096 2307 5 -0.3461818080364907 2307 6 -0.3775915679532673 2307 7 0.13540949524661272 2307 8 -0.3018473004685518 2307 9 -0.1886316152647609 2307 10-11 -0.15286017031538535 2307 12-13 -0.14579008957419148 2307 14-15 0.14716636262883043 2307 16-17 0.02952154850867572 2307 18-19 -0.001766821030990684 2307 20-21 -0.059363761905110835 2307 22-23 -0.0642909555659088 2307 24-25 0.12708275131399915 2307 26-27 -0.2098429395757151 2307 28-29 -0.18906055950009915 2307 30-31 -0.11279425420975997 2307 32-33 -0.1410313054481236 2307 34-35 -0.3511355133561409 2307 36-37 -0.21196324080170825 2307 38-39 -0.36425664966178317 2307 40-41 -0.23938785758252124 2307 42-43 -0.0762463441082204 2307 44-45 -0.05814215009110768 2307 46-47 -0.30272025711690986 2307 48-49 -0.35214854180289734 2307 50-51 -0.30404998485956014 2307 52-53 -0.3069985643357853 2307 54-55 -0.3024300035912333 2307 56-57 -0.25524006480143413 2307 58-59 -0.015429264964218703 2307 60-61 -0.16836141611863553 2307 62-63 -0.11977495106031455 2307 64-65 -0.26675038564641085 2307 66-67 -0.3311954833815989 2307 68-69 -0.23840305776479198 2307 70-71 -0.3809363293733554 2307 72-73 -0.6094466891427857 2307 74-75 -0.4045019125206082 2307 76-77 -0.19115318936297143 2307 78-79 -0.37082368570972335 2307 80-81 -0.1730740220457001 2307 82-83 -0.27381903577926536 2307 84-85 -0.2877343473312237 2307 86-87 -0.17837405938938034 2307 88-89 0.054028882814993295 2307 90-91 -0.17646145452190964 2307 92-93 -0.22939106433617695 2307 94-95 -0.24313866192665046 2307 96-97 -0.25547274270865117 2307 98-99 -0.19718073638955502 2307 100-101 -0.4258180786811465 2201 1 0.13026956116224753 2201 2 0.059725188790110906 2201 3 0.09666978098089984 2201 4 0.25358666016592935 2201 5 0.20509918202336053 2201 6 0.19505590905045267 2201 7 0.5058656834760384 2201 8 0.12679952771034664 2201 9 0.233181231383071 2201 10-11 0.2803891544176764 2201 12-13 0.2627306274608543 2201 14-15 0.5218170235564017 2201 16-17 0.41621173507508047 2201 18-19 0.5054987000752575 2201 20-21 0.3976557743272906 2201 22-23 0.3094357988844223 2201 24-25 0.4332516665014765 2201 26-27 0.3446445494346051 2201 28-29 0.3008799384144183 2201 30-31 0.3822526037348126 2201 32-33 0.4188569603961412 2201 34-35 0.2904919290909618 2201 36-37 0.26414092150147894 2201 38-39 0.29517198316148097 2201 40-41 0.2077150476225924 2201 42-43 0.38066825975955965 2201 44-45 0.3779685673494484 2201 46-47 0.3051937961694975 2201 48-49 0.2998468244507748 2201 50-51 0.3100216333605488 2201 52-53 0.20431719972441442 2201 54-55 0.39971289505873386 2201 56-57 0.42377789368297414 2201 58-59 0.573456704491683 2201 60-61 0.5308739990142826 2201 62-63 0.5597096638065509 2201 64-65 0.5210946631136935 2201 66-67 0.3794879064103682 2201 68-69 0.19956111640286878 2201 70-71 0.27666330307072684 2201 72-73 0.1358886282901679 2201 74-75 0.28851521486603104 2201 76-77 0.4241339302519833 2201 78-79 0.4143293880351493 2201 80-81 0.44458954389049765 2201 82-83 0.3266682308246125 2201 84-85 0.41873178564358327 2201 86-87 0.3775977418143732 2201 88-89 0.3555602891553349 2201 90-91 0.33893224878370987 2201 92-93 0.313160472713939 2201 94-95 0.27462835227099447 2201 96-97 0.2276991996312283 2201 98-99 0.1358651825984758 2201 100-101 0.09820213104144315 1101 1 -0.0058737544921960705 1101 2 -0.22624395719521218 1101 3 -0.11682010098094509 1101 4 -0.004019584296358403 1101 5 -0.07745791602938823 1101 6 9.75074655777064E-4 1101 7 0.25552684079565324 1101 8 -0.10585219947622448 1101 9 -0.04743064262086705 1101 10-11 -0.06590845059270833 1101 12-13 -0.11078266352156874 1101 14-15 0.10161642714700747 1101 16-17 0.06729739107238686 1101 18-19 -0.008764508715273678 1101 20-21 0.019097106674642816 1101 22-23 -0.036814660281393685 1101 24-25 -0.08915803570840097 1101 26-27 -0.1819543840473301 1101 28-29 -0.0984499928336291 1101 30-31 -0.04194295221496702 1101 32-33 0.002134936730662673 1101 34-35 -0.16327477321628692 1101 36-37 -0.14659618304948907 1101 38-39 -0.17449366699321445 1101 40-41 -0.2200979154599949 1101 42-43 -0.09162473479214839 1101 44-45 -0.12802329348450314 1101 46-47 -0.14667463510795642 1101 48-49 -0.2988404192720324 1101 50-51 -0.2742190244830667 1101 52-53 -0.18028982664543491 1101 54-55 0.0555050368394987 1101 56-57 5.98701833283144E-5 1101 58-59 -0.09257939052117337 1101 60-61 -0.13597408434805658 1101 62-63 0.032288040157659736 1101 64-65 -0.03875973432095492 1101 66-67 -0.18744251345760432 1101 68-69 -0.10130033169765085 1101 70-71 0.04579964711776796 1101 72-73 -0.16571539096539567 1101 74-75 -0.19131147353849798 1101 76-77 -0.09884894587592186 1101 78-79 -0.09683077575085974 1101 80-81 -0.06443922278770842 1101 82-83 0.07316541026902357 1101 84-85 0.013595780969495763 1101 86-87 -0.06488548477395639 1101 88-89 -0.01728418644114882 1101 90-91 -0.13505211499324332 1101 92-93 0.06438477027459655 1101 94-95 -0.06225811301510831 1101 96-97 0.10357103620772179 1101 98-99 0.04503085541017171 1101 100-101 -0.29139608802186956 2304 1 -0.02865844681840457 2304 2 -0.27815696900427866 2304 3 -0.2433270166727688 2304 4 -0.10756838965388482 2304 5 -0.12781839819945873 2304 6 -0.06936570336291226 2304 7 0.1575211478886942 2304 8 -0.1291428285504992 2304 9 -0.13563329650697398 2304 10-11 -0.023384440073087376 2304 12-13 -0.08097069409969038 2304 14-15 0.022905858939054724 2304 16-17 -0.015482236517364356 2304 18-19 -0.11685887030345299 2304 20-21 -0.01882479437329465 2304 22-23 0.09639077205113011 2304 24-25 -0.14125726159117136 2304 26-27 -0.16035784668058284 2304 28-29 -0.16387063888282682 2304 30-31 -0.10117280803104478 2304 32-33 -0.20442622354140383 2304 34-35 -0.21927993101503773 2304 36-37 -0.23765611785219676 2304 38-39 -0.24093522431914494 2304 40-41 -0.20384440660878766 2304 42-43 -0.14567800042863155 2304 44-45 -0.1969995455974214 2304 46-47 -0.3160531131993949 2304 48-49 -0.2732896291820879 2304 50-51 -0.474410786501295 2304 52-53 -0.3283910426296046 2304 54-55 -0.10024956862558554 2304 56-57 -0.04045855838823087 2304 58-59 -0.13937683954048197 2304 60-61 -0.10217681161020664 2304 62-63 -0.13187277506805373 2304 64-65 -0.14420003083174038 2304 66-67 -0.12105679350890597 2304 68-69 -0.10601939541937355 2304 70-71 -0.1018363468725525 2304 72-73 -0.19800630728001778 2304 74-75 -0.11325967900215517 2304 76-77 0.004898064274129865 2304 78-79 -0.14267585745329114 2304 80-81 -0.03965592818559216 2304 82-83 -0.09689099085094455 2304 84-85 0.1046735228844824 2304 86-87 0.05996035842860792 2304 88-89 -0.010730274290164488 2304 90-91 -0.09529330740759079 2304 92-93 0.08221532710031454 2304 94-95 0.21452123359809505 2304 96-97 0.06495716330397627 2304 98-99 0.010873861107551619 2304 100-101 0.10227308088709464 2202 1 0.11914023859598899 2202 2 0.031521907201067734 2202 3 0.06078205712126561 2202 4 0.10715828283177586 2202 5 0.07325868413801118 2202 6 0.22823908785635183 2202 7 0.2343843543565356 2202 8 0.2344434440945733 2202 9 0.15018702341760815 2202 10-11 0.25933764803160386 2202 12-13 0.16265229300999806 2202 14-15 0.29905376635486647 2202 16-17 0.31872862296053484 2202 18-19 0.1805057453170491 2202 20-21 0.20415411460148647 2202 22-23 0.2239286080589622 2202 24-25 0.30042708420423736 2202 26-27 0.29886541407562106 2202 28-29 0.24088462084586837 2202 30-31 0.2685942283189817 2202 32-33 0.14774435925688323 2202 34-35 0.12578434351602397 2202 36-37 0.21421948989394934 2202 38-39 0.2125097923378192 2202 40-41 0.2060880736502142 2202 42-43 0.38400606677644333 2202 44-45 0.32348068534528096 2202 46-47 0.21281439037401384 2202 48-49 0.19118058319794073 2202 50-51 0.2513168637228631 2202 52-53 0.28559031720521943 2202 54-55 0.473843684978803 2202 56-57 0.4164548912114654 2202 58-59 0.3499720073327026 2202 60-61 0.33624770995098174 2202 62-63 0.45132694179866206 2202 64-65 0.351726420652291 2202 66-67 0.3275543969369821 2202 68-69 0.30254635540662633 2202 70-71 0.36636443725568313 2202 72-73 0.35409668228820834 2202 74-75 0.3536347536339335 2202 76-77 0.35469870850592145 2202 78-79 0.3638309304302325 2202 80-81 0.42970113944070576 2202 82-83 0.4566289178355625 2202 84-85 0.4630878361476185 2202 86-87 0.40517136054208436 2202 88-89 0.42584507888321 2202 90-91 0.3732057754823863 2202 92-93 0.3080770113425544 2202 94-95 0.4746648111723104 2202 96-97 0.417000977479546 2202 98-99 0.3435320972432585 2202 100-101 0.3278113297698084 1102 1 0.0024707834624209113 1102 2 -0.03922056858574763 1102 3 -0.004952332111532343 1102 4 -0.003646096920235209 1102 5 -0.04880645444879406 1102 6 0.0859808014622061 1102 7 0.16019626296917977 1102 8 0.2519736060115463 1102 9 0.07946127843220552 1102 10-11 0.16432203261696543 1102 12-13 0.19328917885016494 1102 14-15 0.0662293282309534 1102 16-17 0.06867099464458448 1102 18-19 0.09463630133734569 1102 20-21 0.12146584661122972 1102 22-23 0.132206636798756 1102 24-25 0.01813617274735435 1102 26-27 0.22621004999479766 1102 28-29 0.12637529807152958 1102 30-31 0.04095215593037693 1102 32-33 -0.02833291845583119 1102 34-35 -0.04546191529088617 1102 36-37 0.03836389529986661 1102 38-39 0.012393606216946296 1102 40-41 0.19572798962440885 1102 42-43 0.10365313981569102 1102 44-45 0.02661956958672107 1102 46-47 0.045830303225123714 1102 48-49 0.08250845113614957 1102 50-51 0.09947833454620536 1102 52-53 0.1568124092989862 1102 54-55 0.3190335795747572 1102 56-57 0.337627720389996 1102 58-59 0.3270768350984028 1102 60-61 0.3737581667018546 1102 62-63 0.3766276314794723 1102 64-65 0.45626790374488024 1102 66-67 0.45039798253362306 1102 68-69 0.21416868544956458 1102 70-71 0.4075761190730205 1102 72-73 0.29261005763444103 1102 74-75 0.24095327091313123 1102 76-77 0.2568969499128002 1102 78-79 0.14176997454378437 1102 80-81 0.3808332570024753 1102 82-83 0.49230456336564643 1102 84-85 0.339033591171507 1102 86-87 0.2198094175383467 1102 88-89 0.3449014616839463 1102 90-91 0.3763251404555241 1102 92-93 0.3366969061244056 1102 94-95 0.5027116760060224 1102 96-97 0.30323199266739564 1102 98-99 0.20909638999510705 1102 100-101 0.27964660573508127 2305 1 0.19278389925536743 2305 2 0.09561844631915406 2305 3 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-0.7441826303471188 1213 60-61 -1.183827658325825 1213 62-63 -0.9836534136852961 1213 64-65 -0.7388878832713672 1213 66-67 -0.551341713973514 1213 68-69 -0.6692349840006173 1213 70-71 -0.8448587077804106 1213 72-73 -0.5716083135606738 1213 74-75 -1.0779274869211122 1213 76-77 -0.9120863190704753 1213 78-79 -0.8990376935894702 1213 80-81 -1.3581464203492963 1213 82-83 -1.5914762273918832 1213 84-85 -1.5185836606317906 1213 86-87 -1.502523796073934 1213 88-89 -1.508965528229556 1213 90-91 -1.3340696397067013 1213 92-93 -1.434686769910435 1213 94-95 -1.6010318515747137 1213 96-97 -1.5101849957495936 1213 98-99 -1.494237841246104 1213 100-101 -1.456731383549883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1099.0 3 260.0 4 364.0 5 346.0 6 623.0 7 2858.0 8 13449.0 9 38052.0 10 55495.0 11 43202.0 12 25820.0 13 17315.0 14 12533.0 15 10078.0 16 9047.0 17 8493.0 18 8398.0 19 8552.0 20 9091.0 21 9887.0 22 10733.0 23 11562.0 24 13294.0 25 15526.0 26 18289.0 27 22647.0 28 27992.0 29 32671.0 30 39191.0 31 50525.0 32 68241.0 33 97485.0 34 148151.0 35 250140.0 36 488988.0 37 858177.0 38 281453.0 39 996.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.2575783667608 23.846458575536815 19.95090275226917 14.945060305433216 2 32.36766393374466 26.326779124836698 27.87318059274288 13.432376348675762 3 36.87431789280323 20.173230289734384 26.514194849347135 16.438256968115255 4 30.621214438722767 18.61633463456518 29.818049216268278 20.94440171044377 5 35.818170128899865 16.67118674628904 28.640924135821127 18.869718988989973 6 33.42527126925339 17.100973347481798 31.91396678263286 17.55978860063195 7 36.2587629551381 19.15842388694772 25.074055743105983 19.508757414808194 8 29.621982644969307 30.238360245768153 21.559773966567672 18.579883142694868 9 32.8748203394237 25.60183523371016 22.29345865627922 19.229885770586925 10-11 27.26095951621565 30.03659433272432 26.405811821725067 16.296634329334964 12-13 28.864745407487273 25.322084433604562 26.30496877501351 19.50820138389465 14-15 26.140928437861856 25.501472663085593 28.015294055301084 20.34230484375147 16-17 27.68969155821676 25.97567066308844 28.51135365978625 17.82328411890855 18-19 27.115789971240474 26.13782564960051 28.906244229999423 17.84014014915959 20-21 25.462182236442487 28.34215535497021 27.969359552615497 18.226302855971806 22-23 28.294265784877386 26.132164999464575 27.233256896825488 18.340312318832552 24-25 24.760178349373327 29.357030979151993 27.41753805896543 18.465252612509246 26-27 23.417263445185057 30.69334846956559 27.48064247089618 18.408745614353176 28-29 24.389151332102493 29.838744025584663 27.128262202066256 18.643842440246587 30-31 26.32649117275292 27.60943557954324 27.547039661353274 18.51703358635057 32-33 22.69373823406617 30.004585673571444 29.048993454825013 18.252682637537372 34-35 26.991603404966096 27.735775992617107 27.46493062166034 17.807689980756454 36-37 23.499113112924615 29.343337303622818 29.554270108307694 17.603279475144873 38-39 23.132434418694693 31.357416378896648 27.489376460557384 18.020772741851275 40-41 27.383648336469207 26.967592142789332 26.95110312630642 18.697656394435043 42-43 27.1659555339309 26.836679415586268 27.36729811402637 18.630066936456465 44-45 23.921319491659354 28.747979654234502 28.64747589556788 18.683224958538265 46-47 24.578136001948746 29.101507981931167 27.617515157931994 18.702840858188097 48-49 21.26871079388683 29.422786434256814 29.200230921704474 20.10827185015188 50-51 20.187531042439122 27.975173083291942 30.389666262424615 21.447629611844324 52-53 25.37476103107225 27.35446403582424 27.059612289111314 20.211162643992196 54-55 26.46229925093903 27.12917577385417 27.443404971298364 18.965120003908435 56-57 26.987938917960065 26.980942587146888 26.8506775705778 19.18044092431525 58-59 25.419638704571796 27.373563349218134 26.88608158120282 20.32071636500725 60-61 21.92835123016926 27.413649108044492 26.840628426310666 23.817371235475587 62-63 24.61888433045987 27.478149269594603 26.82608750358042 21.076878896365105 64-65 22.023489121325674 28.791946544679604 28.985255969841734 20.19930836415299 66-67 20.93648281256853 28.66888848299319 27.895064980632668 22.499563723805615 68-69 20.854642591945993 27.375456137945402 32.285323964568484 19.48457730554012 70-71 20.222178394701523 31.187916579864737 29.500154115817388 19.089750909616345 72-73 19.94971707297943 34.30660080420866 26.54273336600188 19.200948756810032 74-75 20.301403285210338 34.17015767440794 26.280683682508112 19.24775535787362 76-77 20.600570969918174 33.55681183982875 26.168332350146294 19.67428484010678 78-79 20.26343870782979 33.369069493295164 26.758718574687773 19.60877322418727 80-81 19.68815305385211 33.35156372140439 26.93414025619369 20.026142968549806 82-83 19.63612829819802 32.83739645259513 26.92207542487262 20.604399824334223 84-85 19.727339114458204 32.26747467688906 27.456222395523543 20.54896381312919 86-87 20.05700298005451 31.834639543680076 27.99091697742827 20.11744049883714 88-89 20.55805973698846 32.080596907194554 27.602608461490767 19.758734894326217 90-91 20.964700474405845 31.491991209168642 26.992334300305043 20.55097401612047 92-93 20.77952282563425 31.810495734168708 26.77718300639204 20.632798433805004 94-95 20.43926572460769 31.74323339520687 27.150561755346004 20.666939124839434 96-97 20.70717366203014 32.11551275140522 26.810470902792755 20.366842683771885 98-99 21.075911084927522 31.586158610495225 26.66156974843341 20.676360556143845 100-101 21.21671906253643 31.564456171579664 26.314889617106157 20.903935148777748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 554.0 1 469.5 2 314.0 3 189.0 4 146.5 5 159.0 6 218.0 7 326.5 8 441.0 9 593.0 10 761.0 11 917.5 12 972.5 13 952.5 14 879.5 15 784.5 16 688.0 17 573.5 18 556.0 19 570.5 20 628.0 21 760.5 22 934.5 23 1234.5 24 1740.0 25 2403.0 26 3264.5 27 4434.5 28 5973.0 29 7932.0 30 10254.0 31 13514.0 32 19082.5 33 27912.5 34 41118.5 35 59520.0 36 84655.0 37 116447.0 38 147628.0 39 167416.5 40 174133.0 41 167011.0 42 156869.0 43 154065.5 44 147294.0 45 140801.5 46 142048.5 47 135824.5 48 117363.5 49 98615.5 50 83832.0 51 73967.5 52 66719.0 53 58412.0 54 49747.0 55 41729.0 56 34511.5 57 27869.0 58 22538.0 59 18225.0 60 14695.5 61 11963.0 62 9627.5 63 7682.0 64 6009.0 65 4804.0 66 3849.0 67 3023.0 68 2296.0 69 1735.5 70 1384.5 71 1088.0 72 840.5 73 621.5 74 464.0 75 351.0 76 271.5 77 215.0 78 160.5 79 130.0 80 103.0 81 86.0 82 71.0 83 57.5 84 50.5 85 42.5 86 35.5 87 27.0 88 21.5 89 21.0 90 17.5 91 13.0 92 13.0 93 10.5 94 8.5 95 7.5 96 5.0 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18314119799057405 2 0.1891536884784821 3 0.15997651071200797 4 0.16071423960622983 5 0.16897680322151454 6 0.16550947741867184 7 0.13290186029406612 8 0.11453241082794206 9 0.11578654994811922 10-11 0.09382067212266366 12-13 0.13780775744064142 14-15 0.11705913229065192 16-17 0.10975561623785561 18-19 0.11254054281354309 20-21 0.10468373009008039 22-23 0.10671248454919048 24-25 0.4651933974739425 26-27 0.31194866292170886 28-29 0.404570525591262 30-31 0.2700272185075523 32-33 0.2965485722548278 34-35 0.31569263705988476 36-37 0.2961797078077169 38-39 0.3451649063840477 40-41 0.3402590092374723 42-43 0.3836927978847837 44-45 0.33692078599111847 46-47 0.3613764988345728 48-49 0.3568579093574639 50-51 0.37194446524430075 52-53 0.3627781837335943 54-55 0.3383778005572066 56-57 0.3543680743394652 58-59 0.34612395394653606 60-61 0.3168545600682842 62-63 0.3255228745753909 64-65 0.32749629936743435 66-67 0.3385991192254732 68-69 0.32653725180494597 70-71 0.3139036444913968 72-73 0.299425714942293 74-75 0.3085551100082884 76-77 0.2979871435985604 78-79 0.30781738111406654 80-81 0.3163197066199734 82-83 0.34309926548022646 84-85 0.3409045220199165 86-87 0.33415430263778656 88-89 0.34785761684795735 90-91 0.3362937164310299 92-93 0.3200821239805048 94-95 0.31233597059117535 96-97 0.2975260630396717 98-99 0.32225842421845924 100-101 0.32600239835663514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2711023.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.64657072954861 #Duplication Level Percentage of deduplicated Percentage of total 1 91.77473668466929 54.740483228478254 2 3.9084775064418458 4.662545600656666 3 1.2563446574489985 2.248099514136666 4 0.6664756412301479 1.590119458966211 5 0.44048097504736233 1.3136589816591517 6 0.2700475221983747 0.9664445179886821 7 0.1983206465875018 0.8280402531667866 8 0.195840051568168 0.9344949990031344 9 0.1285892130497049 0.6902915032103595 >10 0.9469701015752693 11.240177826100311 >50 0.10805846519108643 4.56875815651179 >100 0.09741996499597286 11.65450723416728 >500 0.006094603566132749 2.3536756341030274 >1k 0.0020819262488603577 1.8048322605597256 >5k 0.0 0.0 >10k+ 6.204018141779943E-5 0.40387083129183876 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 10914 0.40257865757686306 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004389486920620002 0.0 0.0 2 0.004647692033597649 0.0 0.0 3 0.005459193817241683 0.0 0.0 4 0.005975604043196978 0.0 0.0 5 0.007598607610485046 0.0 0.0 6 0.00918472473306202 0.0 0.0 7 0.011471684305149754 0.0 0.0 8 0.014164394769059502 0.0 0.0 9 0.016598900119991603 0.0 0.0 10-11 0.02677955886025312 0.0 1.8443222355546227E-5 12-13 0.03648069381927044 0.0 3.6886444711092453E-5 14-15 0.04605272622179893 0.0 5.532966706663868E-5 16-17 0.052489410823884565 0.0 7.377288942218491E-5 18-19 0.05957160820841431 0.0 7.377288942218491E-5 20-21 0.06707799970712164 0.0 7.377288942218491E-5 22-23 0.0740495377575181 0.0 9.221611177773114E-5 24-25 0.0816297021456476 0.0 1.1065933413327736E-4 26-27 0.08902543431022164 0.0 1.1065933413327736E-4 28-29 0.09857902349039457 0.0 1.1065933413327736E-4 30-31 0.20471976814656312 0.0 1.2910255648882358E-4 32-33 0.2182386501331785 0.0 1.4754577884436981E-4 34-35 0.22854841142992885 0.0 1.4754577884436981E-4 36-37 0.24061027885045608 0.0 1.6598900119991605E-4 38-39 0.25405538794764926 0.0 1.8443222355546228E-4 40-41 0.2700272185075523 0.0 2.0287544591100851E-4 42-43 0.28701342629701043 0.0 2.2131866826655472E-4 44-45 0.3073194141104668 0.0 2.2131866826655472E-4 46-47 0.33004146405249973 0.0 2.2131866826655472E-4 48-49 0.35530867867959803 0.0 2.2131866826655472E-4 50-51 0.3832501605482506 0.0 2.2131866826655472E-4 52-53 0.4155257996704565 0.0 2.2131866826655472E-4 54-55 0.45128720781786064 0.0 2.5820511297764716E-4 56-57 0.4889113814231749 0.0 2.5820511297764716E-4 58-59 0.5304639613902206 0.0 2.5820511297764716E-4 60-61 0.5763691418331751 0.0 2.5820511297764716E-4 62-63 0.624985475962395 0.0 2.9509155768873963E-4 64-65 0.6751879272141919 0.0 2.9509155768873963E-4 66-67 0.730130286611364 0.0 3.1353478004428586E-4 68-69 0.7890563820373342 0.0 3.319780023998321E-4 70-71 0.8525010669404133 0.0 3.5042122475537833E-4 72-73 0.9218660262196227 0.0 3.6886444711092456E-4 74-75 0.9967455089831403 0.0 3.6886444711092456E-4 76-77 1.075571841330745 0.0 3.6886444711092456E-4 78-79 1.1594700598261247 0.0 3.6886444711092456E-4 80-81 1.2513910800461672 0.0 3.6886444711092456E-4 82-83 1.35328988356056 0.0 3.6886444711092456E-4 84-85 1.4627504082407268 0.0 4.0575089182201703E-4 86-87 1.5782049801864462 0.0 4.0575089182201703E-4 88-89 1.7027151743087388 0.0 4.0575089182201703E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2550 0.0 88.11817 9 CGGGAGA 3640 0.0 84.545586 4 AGAGCGG 2745 0.0 82.72189 8 ATCGGGA 4195 0.0 82.079094 2 TCGGGAG 3900 0.0 80.12508 3 GAGAGCG 3470 0.0 78.281746 7 GTTCGGA 420 0.0 75.657745 2 GATCCGG 605 0.0 73.689964 1 GATCGGG 4855 0.0 73.65768 1 AAGCGGC 780 0.0 72.9313 9 ATTCGGA 515 0.0 71.83156 2 CGGAAAG 1235 0.0 67.96009 5 TCGGGAA 935 0.0 67.45089 3 TCGGAGA 675 0.0 66.73712 4 ATCCGGA 665 0.0 66.32682 2 TTCGGAG 505 0.0 65.72859 3 GGACCGG 540 0.0 64.11579 1 ACCGGAA 420 0.0 63.22464 3 GGATCGG 1240 0.0 61.96254 1 AAAGCGG 990 0.0 61.770596 8 >>END_MODULE