##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2286754 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.199824729726068 33.0 33.0 33.0 27.0 33.0 2 30.812421887094107 33.0 33.0 33.0 27.0 33.0 3 30.654823387211742 33.0 33.0 33.0 27.0 33.0 4 34.13549861506747 37.0 37.0 37.0 27.0 37.0 5 34.237152750142776 37.0 37.0 37.0 27.0 37.0 6 34.658562748769654 37.0 37.0 37.0 27.0 37.0 7 34.06801387468875 37.0 37.0 37.0 27.0 37.0 8 34.364430104856055 37.0 37.0 37.0 27.0 37.0 9 33.86324064591119 37.0 37.0 37.0 27.0 37.0 10-11 33.8550823569129 37.0 37.0 37.0 24.5 37.0 12-13 33.833683028432446 37.0 37.0 37.0 24.5 37.0 14-15 35.333120877890664 40.0 37.0 40.0 24.5 40.0 16-17 35.2552246546852 40.0 37.0 40.0 24.5 40.0 18-19 35.28598834854995 40.0 37.0 40.0 22.0 40.0 20-21 35.33509988394029 40.0 37.0 40.0 24.5 40.0 22-23 35.88871212207347 40.0 37.0 40.0 27.0 40.0 24-25 35.28431086159683 38.5 37.0 40.0 22.0 40.0 26-27 35.17478027807101 37.0 37.0 40.0 24.5 40.0 28-29 34.89799842921451 37.0 37.0 40.0 18.5 40.0 30-31 34.918766732232676 37.0 37.0 40.0 18.5 40.0 32-33 34.72922032715368 37.0 37.0 40.0 15.0 40.0 34-35 34.83163427286013 37.0 37.0 40.0 18.5 40.0 36-37 34.67893857406612 37.0 37.0 40.0 18.5 40.0 38-39 34.78998265663906 37.0 37.0 40.0 22.0 40.0 40-41 34.38314812174812 37.0 37.0 40.0 15.0 40.0 42-43 34.281918606024085 37.0 35.0 40.0 15.0 40.0 44-45 34.15020767428416 37.0 33.0 40.0 15.0 40.0 46-47 34.11495858321446 37.0 33.0 40.0 15.0 40.0 48-49 34.01545793732076 37.0 33.0 40.0 15.0 40.0 50-51 32.95051194837748 37.0 33.0 38.5 15.0 38.5 52-53 33.44406875422543 37.0 33.0 38.5 18.5 40.0 54-55 34.111471325730705 37.0 33.0 40.0 18.5 40.0 56-57 34.285722032190606 37.0 33.0 40.0 18.5 40.0 58-59 33.79409438006887 37.0 33.0 40.0 15.0 40.0 60-61 33.63395406764348 37.0 33.0 40.0 15.0 40.0 62-63 33.246416973579144 37.0 33.0 40.0 15.0 40.0 64-65 33.059812730184355 37.0 33.0 37.0 15.0 40.0 66-67 32.9157543837247 37.0 33.0 37.0 15.0 40.0 68-69 32.94845313488027 37.0 33.0 37.0 18.5 40.0 70-71 32.51303616392494 37.0 33.0 37.0 15.0 38.5 72-73 32.665789586461855 37.0 33.0 37.0 22.0 37.0 74-75 32.65157883182887 37.0 33.0 37.0 22.0 37.0 76-77 32.33614459622679 37.0 33.0 37.0 22.0 37.0 78-79 31.915042020261033 37.0 33.0 37.0 22.0 37.0 80-81 31.48853658941889 33.0 33.0 37.0 15.0 37.0 82-83 31.179521496409322 33.0 33.0 37.0 15.0 37.0 84-85 30.993225331627276 33.0 33.0 37.0 15.0 37.0 86-87 30.82917838997986 33.0 33.0 37.0 15.0 37.0 88-89 30.693024260589464 33.0 33.0 37.0 15.0 37.0 90-91 30.558050844122278 33.0 27.0 37.0 15.0 37.0 92-93 30.417020151708492 33.0 27.0 37.0 15.0 37.0 94-95 30.2747379035961 33.0 27.0 37.0 15.0 37.0 96-97 30.105548082565946 33.0 27.0 37.0 15.0 37.0 98-99 29.948750499616487 33.0 27.0 37.0 15.0 37.0 100-101 28.736570921052284 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -4.1492072712401296E-4 2306 2 0.13798814835279316 2306 3 0.06750759261224104 2306 4 0.20793852572747085 2306 5 0.19483238179164886 2306 6 0.09406698892856724 2306 7 0.4092673758969525 2306 8 0.15083913897985468 2306 9 0.24737678588609668 2306 10-11 0.2867365091702041 2306 12-13 0.2415682661944416 2306 14-15 0.5827672157544086 2306 16-17 0.49680492141252586 2306 18-19 0.5228321658088291 2306 20-21 0.4558958862046083 2306 22-23 0.3370019201297154 2306 24-25 0.4151746963449554 2306 26-27 0.2080590983366477 2306 28-29 0.2715957548112442 2306 30-31 0.1635783523311929 2306 32-33 0.33116885238982974 2306 34-35 0.16577738249191754 2306 36-37 0.20800301887902606 2306 38-39 0.06976763824699361 2306 40-41 0.17464361576045206 2306 42-43 0.27995127638681794 2306 44-45 0.2966820182592116 2306 46-47 0.08876242858837458 2306 48-49 0.1554069267490661 2306 50-51 0.3588257764356726 2306 52-53 0.151031338279644 2306 54-55 0.32576506475211886 2306 56-57 0.38067758220036296 2306 58-59 0.532543864676434 2306 60-61 0.46074859254962774 2306 62-63 0.5515919780018521 2306 64-65 0.3873865709552362 2306 66-67 0.4255477176158706 2306 68-69 0.24418108215513 2306 70-71 0.39412170722841466 2306 72-73 0.2129210022578576 2306 74-75 0.19435856896922843 2306 76-77 0.3441976889505014 2306 78-79 0.2870473363356574 2306 80-81 0.6194987237382286 2306 82-83 0.47043551726699917 2306 84-85 0.42452535285687176 2306 86-87 0.36535498464482785 2306 88-89 0.48515843585242635 2306 90-91 0.3963026417197142 2306 92-93 0.3327612227091805 2306 94-95 0.3418909248116542 2306 96-97 0.3300845278165774 2306 98-99 0.17007449819805842 2306 100-101 0.19391343758383428 2307 1 0.120375417459897 2307 2 0.10335144179082079 2307 3 0.053285315398131416 2307 4 0.26873467734272793 2307 5 0.1994340244779238 2307 6 0.11624508670676903 2307 7 0.5709232340388297 2307 8 0.07292152468510693 2307 9 0.3007565611787655 2307 10-11 0.29254535474643006 2307 12-13 0.25215862375955567 2307 14-15 0.5922763935078308 2307 16-17 0.3995473472618585 2307 18-19 0.4412248576486917 2307 20-21 0.3738509342635865 2307 22-23 0.31701335058658486 2307 24-25 0.4915157128307399 2307 26-27 0.4673993724319345 2307 28-29 0.27844126295593696 2307 30-31 0.446613747467552 2307 32-33 0.45290829165586644 2307 34-35 0.32748659585913487 2307 36-37 0.24137278433861553 2307 38-39 0.2797569475619639 2307 40-41 0.3566566200328012 2307 42-43 0.3021632530964382 2307 44-45 0.28029967275040946 2307 46-47 0.33838599601693886 2307 48-49 0.25419363547143803 2307 50-51 0.08179824513938883 2307 52-53 0.25202508753496033 2307 54-55 0.33231335483171165 2307 56-57 0.37259262889763534 2307 58-59 0.5508283403353218 2307 60-61 0.4418608427784321 2307 62-63 0.4871053141895203 2307 64-65 0.37824695178715473 2307 66-67 0.38235322570818653 2307 68-69 0.3351726906547796 2307 70-71 0.2888582306150198 2307 72-73 0.1725090017184101 2307 74-75 0.1830907964418671 2307 76-77 0.2615209849548137 2307 78-79 0.29334128893003353 2307 80-81 0.3357086631293136 2307 82-83 0.3428155465698204 2307 84-85 0.48533132445225036 2307 86-87 0.4313953329791609 2307 88-89 0.5549748938772474 2307 90-91 0.5298686103821915 2307 92-93 0.25013574627648083 2307 94-95 0.19462101021929 2307 96-97 0.2854515724870126 2307 98-99 0.39953273644125176 2307 100-101 0.46109332914506496 2201 1 0.2731765500953678 2201 2 0.040666677101956594 2201 3 0.05148558679790938 2201 4 0.11616755058209804 2201 5 0.0956330164888044 2201 6 0.2695271660900289 2201 7 0.4567883384712985 2201 8 0.18171544194345302 2201 9 0.2243007271418449 2201 10-11 0.22327020852215895 2201 12-13 0.2440298593811292 2201 14-15 0.42443979545937793 2201 16-17 0.4633324687214895 2201 18-19 0.49095933741482867 2201 20-21 0.4388304852074896 2201 22-23 0.4739101739862335 2201 24-25 0.25459799352960033 2201 26-27 0.24459608788314569 2201 28-29 0.3934839602087763 2201 30-31 0.40890705207735323 2201 32-33 0.3389929970789254 2201 34-35 0.25083791275952905 2201 36-37 0.2475789376348132 2201 38-39 0.2400209273551539 2201 40-41 0.3981544457772088 2201 42-43 0.2972735298348468 2201 44-45 0.1952838097391023 2201 46-47 0.13720914637100634 2201 48-49 0.17786421164328914 2201 50-51 0.24064648938978195 2201 52-53 0.11948163182086802 2201 54-55 0.42656715830911196 2201 56-57 0.5220021462340583 2201 58-59 0.5512228577775815 2201 60-61 0.44206897865705486 2201 62-63 0.4906744917353052 2201 64-65 0.41194585560486274 2201 66-67 0.5031866864316541 2201 68-69 0.4738717355815609 2201 70-71 0.4624467871397897 2201 72-73 0.3383312677889663 2201 74-75 0.4804046676986573 2201 76-77 0.6112345335421878 2201 78-79 0.5057721748833295 2201 80-81 0.4655939767324604 2201 82-83 0.4318168588841722 2201 84-85 0.42733784839493083 2201 86-87 0.5168162966130012 2201 88-89 0.4009220061977139 2201 90-91 0.5462898844559909 2201 92-93 0.49019020261851054 2201 94-95 0.43870148472205983 2201 96-97 0.5114715558831762 2201 98-99 0.3741519183378976 2201 100-101 0.35662411091283275 1101 1 0.02797169966540025 1101 2 -0.27359847131876336 1101 3 -0.040203252281365565 1101 4 -0.10370598168152867 1101 5 -0.16365012436586568 1101 6 -0.06120312330514821 1101 7 0.14717170762403242 1101 8 -0.12660834524671571 1101 9 -0.006622409633571635 1101 10-11 0.013008019180993813 1101 12-13 -0.04575017373854706 1101 14-15 0.2261074072976328 1101 16-17 0.17391436167986285 1101 18-19 0.06408175988872244 1101 20-21 0.21048642584027988 1101 22-23 0.25471827141786463 1101 24-25 -0.021956427040642268 1101 26-27 -0.12389403152143785 1101 28-29 -0.13593241476768725 1101 30-31 0.036051696785463605 1101 32-33 -0.06205185369637434 1101 34-35 -0.2007260449608097 1101 36-37 -0.39117877234841103 1101 38-39 -0.3074932353801998 1101 40-41 -0.3934992933521926 1101 42-43 -0.36042183065863753 1101 44-45 -0.4364848014629885 1101 46-47 -0.4791614864371141 1101 48-49 -0.4120069032832845 1101 50-51 -0.3629697227401749 1101 52-53 -0.28779015510382777 1101 54-55 0.019056335599742624 1101 56-57 -0.037781216119910255 1101 58-59 -0.12147671956613948 1101 60-61 -0.27921124952229803 1101 62-63 -0.08175078799861524 1101 64-65 -0.14493022727310745 1101 66-67 -0.1632067707866014 1101 68-69 -0.09942079080006039 1101 70-71 -0.10500757348265921 1101 72-73 -0.27330640252402816 1101 74-75 -0.16899377689603767 1101 76-77 -0.19496410141778853 1101 78-79 -0.3899781411780907 1101 80-81 -0.0657599100811268 1101 82-83 -0.03213608613921792 1101 84-85 -0.1845829687081597 1101 86-87 -0.2937996441061266 1101 88-89 -0.06101356853226392 1101 90-91 -0.27614483800080336 1101 92-93 -0.21227931222518137 1101 94-95 -0.2650244518476619 1101 96-97 -0.30415779620034655 1101 98-99 -0.4280327500255794 1101 100-101 -0.41956905805956524 2304 1 0.10246743599356734 2304 2 -0.04879455643130015 2304 3 0.058282511328101805 2304 4 0.2808087991541015 2304 5 0.1466579951483169 2304 6 0.31190537281841557 2304 7 0.4432361789771875 2304 8 0.29355787165204816 2304 9 0.20263689655197226 2304 10-11 0.2999726553438151 2304 12-13 0.3732897577297649 2304 14-15 0.4329611173846786 2304 16-17 0.4369582255523454 2304 18-19 0.3935004435813738 2304 20-21 0.4251195748954686 2304 22-23 0.3384934384724616 2304 24-25 0.2416388935361553 2304 26-27 0.26698999429209636 2304 28-29 0.1681557946869603 2304 30-31 0.20619128188040747 2304 32-33 0.20897417068088942 2304 34-35 0.10490053557216328 2304 36-37 0.151963027676139 2304 38-39 0.13034813452534166 2304 40-41 0.26597551517559026 2304 42-43 0.38910242568667996 2304 44-45 0.2962389075341818 2304 46-47 0.19202774845884107 2304 48-49 0.39385696477648935 2304 50-51 0.15829574185010387 2304 52-53 0.17618236530302767 2304 54-55 0.3453506720176023 2304 56-57 0.3967927451351869 2304 58-59 0.3166501580375254 2304 60-61 0.42272400308866764 2304 62-63 0.4875011277076098 2304 64-65 0.4976648150536249 2304 66-67 0.2439975688320004 2304 68-69 0.37189448079882226 2304 70-71 0.2613669511119667 2304 72-73 0.2429792222802334 2304 74-75 0.2715013015712273 2304 76-77 0.37624417552541445 2304 78-79 0.212102608419233 2304 80-81 0.1883870861589294 2304 82-83 0.3019979644609663 2304 84-85 0.34072548662507884 2304 86-87 0.3805529324378618 2304 88-89 0.39785019693663415 2304 90-91 0.3191699573008826 2304 92-93 0.4044047686479182 2304 94-95 0.5702857034926332 2304 96-97 0.33034068572533215 2304 98-99 0.1649468112643504 2304 100-101 0.34724446691122424 2202 1 0.15252608681437252 2202 2 0.12463955032698948 2202 3 0.16505527337583104 2202 4 0.07391104651968305 2202 5 0.11425700671181716 2202 6 0.17009001740710517 2202 7 0.3020458895720779 2202 8 0.13392813506399648 2202 9 0.20902120857117268 2202 10-11 0.21210146869308488 2202 12-13 0.25661205139635257 2202 14-15 0.32412387358202466 2202 16-17 0.20278880236118368 2202 18-19 0.23043861066803117 2202 20-21 0.19531656792997154 2202 22-23 0.20668388821469108 2202 24-25 0.12036331111553267 2202 26-27 0.18270129068668695 2202 28-29 0.07922997875985516 2202 30-31 0.15754592700958625 2202 32-33 -0.06194060799169421 2202 34-35 -0.09766019974426854 2202 36-37 -0.04365979157508093 2202 38-39 -0.04511574066227553 2202 40-41 0.021380221669339505 2202 42-43 0.0016223349661714792 2202 44-45 0.04635983881016159 2202 46-47 -0.00655961141296757 2202 48-49 0.1529454674742894 2202 50-51 0.03912421663703469 2202 52-53 0.0874540874209302 2202 54-55 0.24023487763538043 2202 56-57 0.3232683385012507 2202 58-59 0.20283907134761847 2202 60-61 0.2732239993572705 2202 62-63 0.3368723189943168 2202 64-65 0.1386114327432182 2202 66-67 0.17023344802781537 2202 68-69 0.2816444702265244 2202 70-71 0.30441430167110184 2202 72-73 0.43329271187855767 2202 74-75 0.2622189356918696 2202 76-77 0.2598532216145344 2202 78-79 0.11007562157886852 2202 80-81 0.23331042624571907 2202 82-83 0.15571182670175432 2202 84-85 0.12317221297755765 2202 86-87 0.22742476428154035 2202 88-89 0.3380796169662865 2202 90-91 0.335216672617463 2202 92-93 0.2854609718965868 2202 94-95 0.31519138162027716 2202 96-97 0.27336989941988676 2202 98-99 0.14300369272320523 2202 100-101 0.12385324863632263 1102 1 0.06554225192715535 1102 2 -0.3152657796347711 1102 3 -0.1892317951773741 1102 4 -0.2421174445720311 1102 5 -0.3372543037196891 1102 6 -0.13002923166688873 1102 7 -0.07159641896988234 1102 8 -0.010966567574712371 1102 9 -0.1451948681771853 1102 10-11 -0.015089572184031397 1102 12-13 -0.037958684108765794 1102 14-15 -0.12510647659416918 1102 16-17 -0.08504982962030283 1102 18-19 0.015484755341176992 1102 20-21 -0.12331577816687656 1102 22-23 0.010206239909379633 1102 24-25 -0.20693518261423804 1102 26-27 0.012661548144578205 1102 28-29 -0.1877408159408489 1102 30-31 -0.13720925850207522 1102 32-33 -0.18160244249714452 1102 34-35 -0.21666123137745075 1102 36-37 -0.3551967105307483 1102 38-39 -0.21857241957555829 1102 40-41 -0.3592264687184539 1102 42-43 -0.20532853484429126 1102 44-45 -0.3216102030771495 1102 46-47 -0.3050880864189125 1102 48-49 -0.3777733942546888 1102 50-51 -0.3697287402467069 1102 52-53 -0.31199363494319954 1102 54-55 0.006735968249657276 1102 56-57 0.055887571489492416 1102 58-59 -0.03680373906994561 1102 60-61 -0.13907141663111844 1102 62-63 -0.11456531867294473 1102 64-65 -0.1319276116770638 1102 66-67 -0.09405960442676076 1102 68-69 -0.0666248096248836 1102 70-71 -0.05140348745964474 1102 72-73 0.06427838268677277 1102 74-75 -0.03830835540241395 1102 76-77 -0.15670266780983155 1102 78-79 -0.18277661895252706 1102 80-81 -0.1646729560955542 1102 82-83 0.001966526423579751 1102 84-85 -0.009207020525426657 1102 86-87 -0.1108644833554635 1102 88-89 -0.05337852467844684 1102 90-91 0.09174609561065594 1102 92-93 0.14517011997348206 1102 94-95 0.07819028135454076 1102 96-97 -0.16622465372852702 1102 98-99 -0.03594895295476874 1102 100-101 -0.20580952956683518 2305 1 0.3288870605527521 2305 2 0.23457284906780274 2305 3 0.23545084127102456 2305 4 0.21307871120506405 2305 5 0.22829591263737825 2305 6 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17 7550.0 18 7719.0 19 8100.0 20 8605.0 21 9426.0 22 10031.0 23 11027.0 24 12757.0 25 14191.0 26 17203.0 27 20866.0 28 25559.0 29 30632.0 30 37490.0 31 48549.0 32 66970.0 33 96047.0 34 145508.0 35 246661.0 36 465604.0 37 666729.0 38 135495.0 39 86.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.07054806612698 23.53928397918429 22.310825912658654 14.07934204203007 2 32.07394579230357 25.41173604907756 29.9478093096836 12.566508848935268 3 36.462065718446496 19.630435020639666 28.240799745973348 15.666699514940492 4 30.123226443645855 17.46123305280539 32.34375513261023 20.07178537093853 5 34.59877872131482 15.846093130274545 32.03265678704977 17.522471361360868 6 31.284952660607036 16.44456981071251 36.13268520530089 16.137792323379564 7 35.72458818274965 18.70736235539325 27.907241503122016 17.66080795873508 8 29.581908121268054 29.905483978157427 23.215155836076498 17.297452064498025 9 31.89921963072006 26.44473387546926 23.478279904122935 18.177766589687742 10-11 26.59308374844368 29.11020520359174 27.620360114652964 16.676350933311625 12-13 28.199689312820826 24.500913560433474 27.428751591478107 19.87064553526759 14-15 25.54647984627771 24.67623184256162 29.233700170612824 20.543588140547843 16-17 26.808821643126908 25.582751934845426 29.994799261565184 17.61362716046248 18-19 26.253113480069025 25.737051217253327 29.997301048650954 18.0125342540267 20-21 24.907327882444303 27.943598269653535 28.98139903817111 18.167674809731054 22-23 27.628290897104414 25.580609427862207 28.446452956666523 18.344646718366864 24-25 24.22787886455953 28.56982192735283 28.607273679391916 18.59502552869573 26-27 22.7662866206611 29.708398911447176 28.95179416455676 18.573520303334963 28-29 23.43363801668459 29.06739321758483 28.361587725381494 19.137381040349087 30-31 25.495328004349716 27.223067512639158 28.23702868969267 19.044575793318455 32-33 22.6141488323051 27.006107996536784 29.791593017778368 20.58815015337975 34-35 20.44817054713814 26.944980860123163 33.725621113789046 18.881227478949643 36-37 21.575714752199428 27.71196180999869 30.137784798342608 20.574538639459274 38-39 20.333371437021732 27.365037796152876 28.161595036486293 24.139995730339102 40-41 22.238435551904814 26.58380536281658 29.420830903527833 21.756928181750766 42-43 24.040071651530013 26.672042799519485 30.511207459098994 18.776678089851508 44-45 21.728774307234495 28.739380462244423 30.863551280487428 18.668293950033654 46-47 23.345538699481153 29.234068288606657 28.92283364625768 18.49755936565451 48-49 20.647535546365248 28.814318036643854 30.596502207377878 19.941644209613017 50-51 19.828946906383877 27.673972013484093 30.860137650115878 21.636943430016153 52-53 24.48340061567955 27.000914653682447 28.190447223516955 20.325237507121045 54-55 25.91644091312372 26.885206627018288 28.262763960520115 18.93558849933788 56-57 26.346210348994457 26.87378230892769 27.36227405172325 19.4177332903546 58-59 24.497997783530682 26.827199426728264 27.717297697807602 20.957505091933452 60-61 21.022721041343406 27.142833328288408 27.500694226013668 24.33375140435452 62-63 23.611883761415726 27.277288058852843 27.748439002646414 21.362389177085014 64-65 21.32262000395739 28.58757089412924 29.81076471989607 20.279044382017297 66-67 20.776368360534278 28.387418930840365 28.50271095313703 22.333501755488328 68-69 20.878343457777866 26.950684902658793 32.7335517627765 19.43741987678684 70-71 20.361162229089942 31.198376020196662 29.58869706547464 18.851764685238763 72-73 20.11191280319312 34.08789859204351 26.971818939427166 18.828369665336197 74-75 20.85144508353575 33.52712238933637 26.536306661475955 19.085125865651918 76-77 20.920423087947267 32.80045650093872 26.763244744460575 19.515875666653436 78-79 20.794717412168882 32.51758113729956 27.345028942201573 19.342672508329986 80-81 19.877527599939285 32.682929932170346 27.497045448762776 19.942497019127593 82-83 19.29224590846601 32.16575182992945 27.90826152643826 20.633740735166285 84-85 19.435900023695226 31.572266053516106 28.209979200856537 20.781854721932127 86-87 19.425145890921854 31.539906103286384 28.9772497915844 20.057698214207363 88-89 19.820941199650957 31.68970835365649 28.760475064886 19.728875381806557 90-91 20.541664399658107 31.032773045823998 27.94738902042414 20.47817353409376 92-93 19.984790130562715 31.732955579898757 27.636973275465344 20.645281014073188 94-95 19.821873274103982 31.78583382354812 27.887913430920076 20.504379471427825 96-97 20.327498625517197 32.225179647698184 27.319981131152744 20.127340595631875 98-99 20.71480058244343 31.39107285675867 27.234121989345265 20.66000457145264 100-101 20.750717101724465 31.471442723556297 26.806464576622613 20.97137559809662 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 468.0 1 402.5 2 256.5 3 151.0 4 136.0 5 150.5 6 197.0 7 310.0 8 434.0 9 551.0 10 728.5 11 846.0 12 855.0 13 795.0 14 697.0 15 643.5 16 557.0 17 478.5 18 465.0 19 469.0 20 493.0 21 547.0 22 640.5 23 793.5 24 1010.0 25 1272.5 26 1751.5 27 2493.5 28 3256.0 29 4078.0 30 5121.0 31 6637.0 32 10716.5 33 18885.5 34 32712.0 35 52753.5 36 80387.5 37 114139.0 38 142600.0 39 157513.5 40 161005.0 41 149708.5 42 139632.5 43 142600.5 44 139636.5 45 130663.0 46 125725.5 47 113147.0 48 92131.0 49 77891.5 50 67721.0 51 57336.0 52 47849.5 53 40701.5 54 33654.5 55 25756.0 56 20086.0 57 16267.0 58 13180.0 59 10495.5 60 8113.0 61 6222.5 62 4737.0 63 3617.0 64 2855.0 65 2140.0 66 1579.5 67 1262.5 68 1015.0 69 741.5 70 510.5 71 376.0 72 284.5 73 213.5 74 156.5 75 119.0 76 91.5 77 72.5 78 52.0 79 37.5 80 27.5 81 31.5 82 25.5 83 16.5 84 16.0 85 15.5 86 17.5 87 12.0 88 7.5 89 10.0 90 9.0 91 6.0 92 5.0 93 2.5 94 2.5 95 3.5 96 1.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1773255890226933 2 0.18204844071552953 3 0.15432355207425022 4 0.1556354553222603 5 0.16341941459378664 6 0.1603145769068295 7 0.13005334198606408 8 0.11330471051980232 9 0.11277994922059828 10-11 0.09272969458017785 12-13 0.13584758133144187 14-15 0.1152288352835504 16-17 0.10770725666162605 18-19 0.11079022929444968 20-21 0.10302813507705683 22-23 0.10344357110559335 24-25 0.45881629593738543 26-27 0.3074226611170244 28-29 0.3998462449393332 30-31 0.2686777851924606 32-33 0.2970148953494779 34-35 0.3157313816877548 36-37 0.2967306496457424 38-39 0.34479003863117763 40-41 0.3395642906932709 42-43 0.3841471360714795 44-45 0.3367218336559158 46-47 0.36068593298623286 48-49 0.3562035968888652 50-51 0.37231814178525546 52-53 0.362784978183049 54-55 0.3375964358212558 56-57 0.35454185277471906 58-59 0.34566464079651765 60-61 0.31647479352829383 62-63 0.32543946572302923 64-65 0.3268825592958403 66-67 0.33928004498953535 68-69 0.32587676680569927 70-71 0.31350114616613767 72-73 0.29972616206203206 74-75 0.3079255573620949 76-77 0.2974740614862814 78-79 0.3077725019831604 80-81 0.3157313816877548 82-83 0.3428877789215631 84-85 0.341707065998354 86-87 0.3347102486756337 88-89 0.34763249566853277 90-91 0.33610961214017776 92-93 0.319798281756586 94-95 0.3122767031346616 96-97 0.29760525181108244 98-99 0.3232092302014121 100-101 0.32535200550649523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2286754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.70867261814457 #Duplication Level Percentage of deduplicated Percentage of total 1 87.21820586554522 37.24973800653507 2 5.438710452961944 4.645602084008648 3 2.006308709701253 2.5706034556068857 4 1.1213678718216775 1.9156853328855008 5 0.6703952434306387 1.431584548822024 6 0.45802697010297433 1.1737034349845172 7 0.37518776332433357 1.1216639947907008 8 0.33854443493430164 1.1567026750643066 9 0.2446501285830882 0.9403814022877879 >10 1.6507077270891428 13.98798291767996 >50 0.2312218405572602 7.091836526964558 >100 0.22809587659921188 19.633537647120963 >500 0.014411351080327504 3.9482914379361205 >1k 0.004062168937252721 2.8357637698794567 >5k 1.0359533142325611E-4 0.2969227654335076 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 6711 0.2934727565798507 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3517 0.1537987907750462 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3184 0.13923666472213453 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3148 0.13766238082452245 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2778 0.12148224076573169 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2722 0.11903335470277956 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2571 0.11243010835446227 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2562 0.11203653738005924 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2424 0.10600178243921296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004722851692836221 0.0 0.0 2 0.00533507320857425 0.0 0.0 3 0.006034754940846283 0.0 0.0 4 0.006515786131783305 0.0 0.0 5 0.007084277539254332 0.0 0.0 6 0.008090070029395378 0.0 0.0 7 0.010101655009677473 0.0 0.0 8 0.011238637824619526 0.0 0.0 9 0.013075302371833613 0.0 0.0 10-11 0.02031263529002245 0.0 0.0 12-13 0.026959611746606764 0.0 0.0 14-15 0.03299436668745305 0.0 0.0 16-17 0.03819824957122629 0.0 0.0 18-19 0.04386129859180306 0.0 0.0 20-21 0.04983045837024883 0.0 0.0 22-23 0.056324379447898636 0.0 0.0 24-25 0.06336492687888597 0.0 0.0 26-27 0.0704492044181403 0.0 4.373010826700205E-5 28-29 0.07783959271526364 0.0 4.373010826700205E-5 30-31 0.13593504154797587 0.0 4.373010826700205E-5 32-33 0.14608042666592036 0.0 4.373010826700205E-5 34-35 0.15513255907718976 0.0 4.373010826700205E-5 36-37 0.1663274667935423 0.0 4.373010826700205E-5 38-39 0.1791403885157739 0.0 4.373010826700205E-5 40-41 0.1942272758678896 0.0 4.373010826700205E-5 42-43 0.21147880357922189 0.0 4.373010826700205E-5 44-45 0.2308949716497708 0.0 4.373010826700205E-5 46-47 0.25256324029607036 0.0 4.373010826700205E-5 48-49 0.2761993638143849 0.0 4.373010826700205E-5 50-51 0.30337762610232666 0.0 4.373010826700205E-5 52-53 0.33164914109694354 0.0 4.373010826700205E-5 54-55 0.363528390023588 0.0 4.373010826700205E-5 56-57 0.3968725975771771 0.0 4.373010826700205E-5 58-59 0.4340869197123958 0.0 4.373010826700205E-5 60-61 0.4739469133977682 0.0 4.373010826700205E-5 62-63 0.5144191285988786 0.0 6.559516240050308E-5 64-65 0.5588270535440192 0.0 8.74602165340041E-5 66-67 0.6064272763926509 0.0 8.74602165340041E-5 68-69 0.6569574164951718 0.0 8.74602165340041E-5 70-71 0.7111827507462543 0.0 8.74602165340041E-5 72-73 0.7679662963309565 0.0 8.74602165340041E-5 74-75 0.8299537247994319 0.0 8.74602165340041E-5 76-77 0.8980852334794212 0.0 8.74602165340041E-5 78-79 0.971267569664249 0.0 8.74602165340041E-5 80-81 1.0497193838952508 0.0 8.74602165340041E-5 82-83 1.1353429358820406 0.0 8.74602165340041E-5 84-85 1.228575526707289 0.0 8.74602165340041E-5 86-87 1.3304448139152703 0.0 8.74602165340041E-5 88-89 1.4349816377275388 0.0 8.74602165340041E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1655 0.0 87.35182 9 CGGGAGA 2405 0.0 85.14473 4 AGAGCGG 1830 0.0 81.588615 8 TCGGGAG 2575 0.0 78.97128 3 AAGCGGC 645 0.0 78.63107 9 GAGAGCG 2340 0.0 76.9729 7 GTTCGGA 250 0.0 75.85019 2 TCGGGAA 765 0.0 73.73517 3 CGGAAAG 765 0.0 73.11555 5 ATTCGGA 375 0.0 70.79351 2 TCGGAGA 470 0.0 70.597496 4 GATCCGG 445 0.0 69.25132 1 ATCGGGA 3565 0.0 67.95135 2 AATCGGA 475 0.0 66.867935 2 GGATCGG 655 0.0 66.59188 1 GAACCGG 300 0.0 66.37473 1 GATCGGG 3850 0.0 65.512726 1 AACCGGA 330 0.0 63.208492 2 GAATCGG 565 0.0 62.095196 1 ACCGGAA 245 0.0 61.911736 3 >>END_MODULE