FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286754
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT67110.2934727565798507Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA35170.1537987907750462No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31840.13923666472213453No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG31480.13766238082452245No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG27780.12148224076573169No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC27220.11903335470277956No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25710.11243010835446227No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT25620.11203653738005924No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT24240.10600178243921296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC16550.087.351829
CGGGAGA24050.085.144734
AGAGCGG18300.081.5886158
TCGGGAG25750.078.971283
AAGCGGC6450.078.631079
GAGAGCG23400.076.97297
GTTCGGA2500.075.850192
TCGGGAA7650.073.735173
CGGAAAG7650.073.115555
ATTCGGA3750.070.793512
TCGGAGA4700.070.5974964
GATCCGG4450.069.251321
ATCGGGA35650.067.951352
AATCGGA4750.066.8679352
GGATCGG6550.066.591881
GAACCGG3000.066.374731
GATCGGG38500.065.5127261
AACCGGA3300.063.2084922
GAATCGG5650.062.0951961
ACCGGAA2450.061.9117363