##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34042-1.34200000035847.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5224388 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10853596631797 33.0 33.0 33.0 27.0 33.0 2 30.586079173292642 33.0 33.0 33.0 27.0 33.0 3 30.44952155161523 33.0 33.0 33.0 22.0 33.0 4 33.76280417916893 37.0 37.0 37.0 22.0 37.0 5 33.884371911121455 37.0 37.0 37.0 22.0 37.0 6 34.51443192963463 37.0 37.0 37.0 27.0 37.0 7 33.72974652724874 37.0 37.0 37.0 22.0 37.0 8 34.20434125489914 37.0 37.0 37.0 27.0 37.0 9 33.505558354394815 37.0 37.0 37.0 15.0 37.0 10-11 33.54721136714961 37.0 37.0 37.0 18.5 37.0 12-13 33.52257326599785 37.0 37.0 37.0 18.5 37.0 14-15 35.038964468182684 40.0 37.0 40.0 15.0 40.0 16-17 34.97748798519559 40.0 37.0 40.0 18.5 40.0 18-19 35.05752941779975 40.0 37.0 40.0 18.5 40.0 20-21 35.076113699824745 40.0 37.0 40.0 18.5 40.0 22-23 35.92234363144544 40.0 37.0 40.0 27.0 40.0 24-25 35.13492948839175 40.0 37.0 40.0 22.0 40.0 26-27 35.071506557323076 40.0 37.0 40.0 21.0 40.0 28-29 34.708896908116316 40.0 37.0 40.0 15.0 40.0 30-31 34.66728456998217 40.0 37.0 40.0 15.0 40.0 32-33 34.47011047035557 40.0 37.0 40.0 15.0 40.0 34-35 34.82310932878646 38.5 37.0 40.0 18.5 40.0 36-37 34.45739328702233 37.0 37.0 40.0 15.0 40.0 38-39 34.4144882615916 37.0 37.0 40.0 15.0 40.0 40-41 34.1707116699602 37.0 37.0 40.0 15.0 40.0 42-43 34.082332131533875 37.0 37.0 40.0 15.0 40.0 44-45 33.92733723069573 37.0 33.0 40.0 15.0 40.0 46-47 33.89543741774156 37.0 33.0 40.0 15.0 40.0 48-49 33.79317280799206 37.0 33.0 40.0 15.0 40.0 50-51 32.80366331903373 37.0 33.0 38.5 15.0 38.5 52-53 33.27271931181222 37.0 33.0 38.5 15.0 40.0 54-55 33.86221343054919 37.0 33.0 40.0 15.0 40.0 56-57 34.15990226989266 37.0 33.0 40.0 18.5 40.0 58-59 33.5276811370059 37.0 33.0 40.0 15.0 40.0 60-61 33.38647847365088 37.0 33.0 40.0 15.0 40.0 62-63 32.92331321869662 37.0 33.0 40.0 10.5 40.0 64-65 32.75001713119316 37.0 33.0 37.0 10.5 40.0 66-67 32.6239978730523 37.0 33.0 37.0 15.0 40.0 68-69 32.777938296313366 37.0 33.0 37.0 15.0 40.0 70-71 32.29775190893172 37.0 33.0 37.0 15.0 38.5 72-73 32.64318806336742 37.0 33.0 37.0 18.5 37.0 74-75 32.75916375276874 37.0 33.0 37.0 22.0 37.0 76-77 32.45717756797543 37.0 33.0 37.0 22.0 37.0 78-79 31.989452640194415 37.0 33.0 37.0 22.0 37.0 80-81 31.479740593539375 37.0 33.0 37.0 15.0 37.0 82-83 31.091742898881172 33.0 33.0 37.0 15.0 37.0 84-85 30.888490671060417 33.0 33.0 37.0 15.0 37.0 86-87 30.712487472216843 33.0 33.0 37.0 15.0 37.0 88-89 30.58289698238339 33.0 33.0 37.0 15.0 37.0 90-91 30.446916270384207 33.0 30.0 37.0 15.0 37.0 92-93 30.31868574845513 33.0 27.0 37.0 15.0 37.0 94-95 30.186855379041525 33.0 27.0 37.0 10.5 37.0 96-97 30.02695358767381 33.0 27.0 37.0 6.0 37.0 98-99 29.895266775744833 33.0 27.0 37.0 6.0 37.0 100-101 28.740396961328294 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08958891086509979 2306 2 -0.01249888651100406 2306 3 -0.0798351916408464 2306 4 -0.0032237210637120484 2306 5 -0.05773064974926001 2306 6 -0.1295413574224611 2306 7 0.4329541347213919 2306 8 -0.05182761881540898 2306 9 0.18762813984724147 2306 10-11 0.18468866325162736 2306 12-13 0.19509226141978075 2306 14-15 0.5641787636145139 2306 16-17 0.4404708468519587 2306 18-19 0.4734034449368423 2306 20-21 0.3886903335287286 2306 22-23 0.20428674471504138 2306 24-25 0.5343774444383342 2306 26-27 0.281826318054442 2306 28-29 0.33343874025494813 2306 30-31 0.43629265512237936 2306 32-33 0.3794256644726417 2306 34-35 0.17397595658151488 2306 36-37 0.452338261698614 2306 38-39 0.21074769148537342 2306 40-41 0.30187945286721884 2306 42-43 0.34497377416516173 2306 44-45 0.3796303380222028 2306 46-47 0.348569023214111 2306 48-49 0.40726150816130513 2306 50-51 0.3783389298431459 2306 52-53 0.23701875719680032 2306 54-55 0.45186484141132155 2306 56-57 0.40176274557006053 2306 58-59 0.600968512331697 2306 60-61 0.3993243301375955 2306 62-63 0.5143734480452054 2306 64-65 0.4576912101672619 2306 66-67 0.5111580129442572 2306 68-69 0.3734651261862396 2306 70-71 0.36893842121356357 2306 72-73 0.18395164314064516 2306 74-75 0.20657764232881703 2306 76-77 0.5124375952372944 2306 78-79 0.41560179831554933 2306 80-81 0.6654152022192719 2306 82-83 0.5287282794411077 2306 84-85 0.4976483731660011 2306 86-87 0.5669566091011227 2306 88-89 0.5162781695053269 2306 90-91 0.5553676716026423 2306 92-93 0.47817807074814667 2306 94-95 0.5269049534531405 2306 96-97 0.4626903337537911 2306 98-99 0.5560599772685961 2306 100-101 0.38782059062565466 2307 1 -0.03389037811076534 2307 2 -0.060643863679953114 2307 3 -0.15540014364904664 2307 4 0.03465252532524943 2307 5 -0.09706295801503018 2307 6 -0.15404687880909762 2307 7 0.43654012391159114 2307 8 -0.2707960280969246 2307 9 -0.025684925104762613 2307 10-11 0.0509272477265128 2307 12-13 -0.0023867130467252196 2307 14-15 0.36080506824595915 2307 16-17 0.2805183727116187 2307 18-19 0.2808206241372915 2307 20-21 0.19655015553972532 2307 22-23 0.0792622828755114 2307 24-25 0.1968532738111719 2307 26-27 -0.10756073813789158 2307 28-29 0.059435851428190745 2307 30-31 0.15196745243856213 2307 32-33 0.10074415762331768 2307 34-35 -0.140843549988233 2307 36-37 0.038419766218233065 2307 38-39 -0.0315928406178827 2307 40-41 0.08891584276563691 2307 42-43 0.0725635258192554 2307 44-45 0.10779552532999759 2307 46-47 0.031075604613789665 2307 48-49 0.028821125158792427 2307 50-51 -0.026631191767144458 2307 52-53 -0.06692658806459661 2307 54-55 0.012773948204724661 2307 56-57 0.06944865555048807 2307 58-59 0.32398040544510565 2307 60-61 0.21942730283352319 2307 62-63 0.15808296952503298 2307 64-65 0.1568697000563688 2307 66-67 0.12299142781705541 2307 68-69 -0.007739153470829763 2307 70-71 0.11469807781593033 2307 72-73 -0.25259871330330697 2307 74-75 -0.11418987700768213 2307 76-77 0.11334472178653954 2307 78-79 0.11483736583021908 2307 80-81 0.24135145000605718 2307 82-83 0.2386131923103676 2307 84-85 0.17297773436253294 2307 86-87 0.14512645580692762 2307 88-89 0.08215885397089195 2307 90-91 0.15091503438337028 2307 92-93 0.2699021062527578 2307 94-95 0.23172568311486685 2307 96-97 0.12574917030211097 2307 98-99 0.07419435430708177 2307 100-101 -0.003123869350115882 2201 1 0.07674654655039603 2201 2 -0.021784632307468854 2201 3 0.04599070515514114 2201 4 0.11626630893452017 2201 5 0.05424784731393828 2201 6 0.03801942681712234 2201 7 0.4395088436880883 2201 8 -0.019743371670280396 2201 9 0.1584003423227287 2201 10-11 0.2038193131293653 2201 12-13 0.25036582200865354 2201 14-15 0.4504540194818176 2201 16-17 0.40735808877889923 2201 18-19 0.40147337989865406 2201 20-21 0.3715070480917291 2201 22-23 0.2608141968817179 2201 24-25 0.33384231310152046 2201 26-27 0.27135876152415506 2201 28-29 0.2609406939215404 2201 30-31 0.36010202137814673 2201 32-33 0.3247363919928645 2201 34-35 0.17848396346346362 2201 36-37 0.20527530006610561 2201 38-39 0.22170224667960525 2201 40-41 0.25627866887972317 2201 42-43 0.25950992014816876 2201 44-45 0.3135600911039518 2201 46-47 0.2189973646141965 2201 48-49 0.2498601053026519 2201 50-51 0.21518216700567194 2201 52-53 0.18521224581511575 2201 54-55 0.332064351734239 2201 56-57 0.3902439903707844 2201 58-59 0.4536900171591398 2201 60-61 0.4061111157888604 2201 62-63 0.48831665621783316 2201 64-65 0.3926559338809952 2201 66-67 0.3779221266098389 2201 68-69 0.20098669529561874 2201 70-71 0.32406718989944494 2201 72-73 0.0537459882211877 2201 74-75 0.2349286531937267 2201 76-77 0.45006938895530624 2201 78-79 0.3273339600791374 2201 80-81 0.4866264282395889 2201 82-83 0.4714032901547647 2201 84-85 0.46542020066354084 2201 86-87 0.5513123875427262 2201 88-89 0.5015950258667168 2201 90-91 0.5571620513450064 2201 92-93 0.41331027410592824 2201 94-95 0.5008220864789266 2201 96-97 0.42481984609340273 2201 98-99 0.30315846727338425 2201 100-101 0.2461665282595753 1101 1 -0.06282499406418296 1101 2 -0.3034944719156414 1101 3 -0.2162158511102561 1101 4 -0.1735685197916439 1101 5 -0.219997366629336 1101 6 -0.1836698409775721 1101 7 0.12362019464272578 1101 8 -0.04548817618422163 1101 9 -0.1408878447539763 1101 10-11 -0.07159690457832113 1101 12-13 -0.1491815794874114 1101 14-15 0.04976568511422386 1101 16-17 0.039679985692025355 1101 18-19 0.012599716591374488 1101 20-21 0.017622147906493524 1101 22-23 0.042150791915645414 1101 24-25 0.019112307188350997 1101 26-27 -0.049999566956856256 1101 28-29 -0.08581600909645459 1101 30-31 0.042313556453031254 1101 32-33 -0.023387855487193576 1101 34-35 -0.18122505396330268 1101 36-37 -0.16893679269924178 1101 38-39 -0.19331907142365878 1101 40-41 -0.17533098315254136 1101 42-43 -0.1982633691104283 1101 44-45 -0.25450771746361767 1101 46-47 -0.2166961140358694 1101 48-49 -0.18803569244125384 1101 50-51 -0.2149442423888388 1101 52-53 -0.2433446623362201 1101 54-55 0.0467689651873755 1101 56-57 -0.038310691537652986 1101 58-59 -0.14804944942242315 1101 60-61 -0.15402950700359241 1101 62-63 0.051927061318281176 1101 64-65 -0.018165294561171663 1101 66-67 -0.14937503375421102 1101 68-69 -0.10702879589928926 1101 70-71 -0.01656428821818423 1101 72-73 -0.14107581731629892 1101 74-75 0.01642836205432019 1101 76-77 0.022023386935337896 1101 78-79 9.543905826845389E-4 1101 80-81 0.13566402011198164 1101 82-83 0.1800568425007789 1101 84-85 -0.01754824370570418 1101 86-87 0.02609832598119155 1101 88-89 0.1283092495120215 1101 90-91 -0.06879073077412912 1101 92-93 -0.07307020316599022 1101 94-95 -0.06972917911025434 1101 96-97 0.0355910472026153 1101 98-99 0.0696510727577575 1101 100-101 0.03404412397572543 2304 1 -0.004993911534246109 2304 2 -0.17227944036344667 2304 3 -0.14293468751931115 2304 4 -0.016504598507680157 2304 5 -0.14245542664141908 2304 6 -0.04829220897910602 2304 7 0.09960078980688536 2304 8 -0.12296666098448839 2304 9 0.012731178201356386 2304 10-11 -0.004402470340572506 2304 12-13 -0.11641631375564288 2304 14-15 0.007664993943556908 2304 16-17 0.005849959870552368 2304 18-19 0.11124789959996662 2304 20-21 0.1290622083158368 2304 22-23 0.1370316524063 2304 24-25 -0.06726840166835757 2304 26-27 0.04413216313925972 2304 28-29 -0.012220023966037274 2304 30-31 -0.007536539645663254 2304 32-33 0.0161750172895907 2304 34-35 -0.2279002355977866 2304 36-37 -0.09770921258437681 2304 38-39 -0.17431813384333594 2304 40-41 0.023722732382168488 2304 42-43 -0.0373187440581475 2304 44-45 -0.039784940607134445 2304 46-47 -0.016000046545414648 2304 48-49 -0.06063608363650275 2304 50-51 -0.0282910773099303 2304 52-53 -0.16258186273643815 2304 54-55 0.07179658914618159 2304 56-57 0.18447529410878616 2304 58-59 0.13901286991735873 2304 60-61 0.12462748430341009 2304 62-63 0.17665523665478844 2304 64-65 0.073827995711234 2304 66-67 0.09320187662707013 2304 68-69 0.05373860360295879 2304 70-71 0.08719473124831012 2304 72-73 0.00770262243079145 2304 74-75 0.09289270125653815 2304 76-77 0.24367418322012924 2304 78-79 0.15821262841296146 2304 80-81 0.14062873004814236 2304 82-83 0.19021577858955396 2304 84-85 0.09300206721872684 2304 86-87 0.2748728925672985 2304 88-89 0.26160333641042754 2304 90-91 0.11443121475953077 2304 92-93 0.14343817872552833 2304 94-95 0.27320962338978205 2304 96-97 0.31234779509312105 2304 98-99 0.13763229145893519 2304 100-101 0.21054182504216712 2202 1 0.16216179359688 2202 2 -0.08649421223217146 2202 3 -0.0910154268868979 2202 4 -0.04863276750008083 2202 5 -0.14014659874344204 2202 6 0.10890431718966909 2202 7 0.10720225855887122 2202 8 0.12845332173873203 2202 9 0.0050846494323337765 2202 10-11 0.05686790128235941 2202 12-13 0.04385646031450818 2202 14-15 0.08121303583548212 2202 16-17 0.10810462422833922 2202 18-19 0.0056098039988725645 2202 20-21 0.06248245310855083 2202 22-23 0.10255344471181616 2202 24-25 0.0693529239618158 2202 26-27 0.010871491102214748 2202 28-29 0.022934649253002704 2202 30-31 0.09198424142047656 2202 32-33 -0.13641252054171815 2202 34-35 -0.13665779257179622 2202 36-37 -0.12243329938686998 2202 38-39 -0.11758148902404031 2202 40-41 -0.1057954764406972 2202 42-43 -0.0692544524043015 2202 44-45 -0.02332129165879593 2202 46-47 -0.01687319877515847 2202 48-49 -0.004390388429349912 2202 50-51 -0.05768288659397314 2202 52-53 -0.07204931686055005 2202 54-55 0.09219208796810818 2202 56-57 0.19447433799660274 2202 58-59 0.06478031414657437 2202 60-61 0.032574606992518795 2202 62-63 0.09423443596035241 2202 64-65 0.07943000023058744 2202 66-67 0.15593543367773322 2202 68-69 0.10583662383699988 2202 70-71 0.2162455702529016 2202 72-73 0.17141182844529368 2202 74-75 0.21853381054910415 2202 76-77 0.28816739692314997 2202 78-79 0.1566315978510744 2202 80-81 0.08196699425015197 2202 82-83 0.19878951007470747 2202 84-85 0.18317588260056894 2202 86-87 0.22788159351123127 2202 88-89 0.27412253806105724 2202 90-91 0.24312967581728984 2202 92-93 0.14388592563315328 2202 94-95 0.1685599949007397 2202 96-97 0.12530258767545277 2202 98-99 0.24676625300535449 2202 100-101 0.2308113227053461 1102 1 0.16391408386727946 1102 2 -0.0046962116919075925 1102 3 0.021680354856453476 1102 4 0.11731377064165827 1102 5 0.07574882697839058 1102 6 0.2315009609647447 1102 7 0.2359546165996136 1102 8 0.2960173585916124 1102 9 0.20896310050702027 1102 10-11 0.2753664921137684 1102 12-13 0.33763154239426996 1102 14-15 0.2880928589474081 1102 16-17 0.29925208993977037 1102 18-19 0.33449039630664146 1102 20-21 0.32536896815443583 1102 22-23 0.26978781906648663 1102 24-25 0.05763132711282992 1102 26-27 0.23484042681673145 1102 28-29 0.006866711310664186 1102 30-31 0.09230369092355772 1102 32-33 0.16695983042833262 1102 34-35 0.03576331004313715 1102 36-37 0.08008427632875481 1102 38-39 0.16569346043661426 1102 40-41 0.19654363904599848 1102 42-43 0.10588959951986254 1102 44-45 0.10329065732576481 1102 46-47 0.13602330403061558 1102 48-49 0.21715284857271655 1102 50-51 8.0561309906102E-4 1102 52-53 0.11779458112955155 1102 54-55 0.2583284280072107 1102 56-57 0.3146672072883874 1102 58-59 0.3332362825784756 1102 60-61 0.3162019172706536 1102 62-63 0.3670469834514094 1102 64-65 0.3402186811755712 1102 66-67 0.24161419757471236 1102 68-69 0.13894398279192188 1102 70-71 0.34022351537731055 1102 72-73 0.33099135069586083 1102 74-75 0.1681211930603297 1102 76-77 0.25539891463565567 1102 78-79 0.12933483383233835 1102 80-81 0.1141270666929941 1102 82-83 0.22704644546000807 1102 84-85 0.19803456696003963 1102 86-87 0.25544139833864676 1102 88-89 0.39691782334297443 1102 90-91 0.4362582378490494 1102 92-93 0.29918884981093186 1102 94-95 0.3445403138348482 1102 96-97 0.31007387326780744 1102 98-99 0.16065756945742748 1102 100-101 0.2978807859048267 2305 1 0.14663434114483564 2305 2 -8.063283218078254E-4 2305 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132433.0 11 100004.0 12 60914.0 13 40071.0 14 28736.0 15 22331.0 16 19650.0 17 17994.0 18 17676.0 19 17750.0 20 18721.0 21 20049.0 22 20641.0 23 21918.0 24 24298.0 25 27573.0 26 32182.0 27 39046.0 28 49506.0 29 61620.0 30 72624.0 31 92644.0 32 129249.0 33 185172.0 34 283629.0 35 486648.0 36 998587.0 37 1697786.0 38 375350.0 39 63.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.3626354618258 23.938603103825926 22.581640493984175 14.1171209403641 2 30.375055831000207 27.119032171888712 29.7324887230489 12.773423274062178 3 34.440041258301505 19.86562293900213 29.633410788509025 16.060925014187337 4 27.457408812324225 17.651298197460836 33.27017918025756 21.621113809957382 5 34.51054215134896 16.46101463269474 31.038220331768496 17.99022288418781 6 31.16477911113736 16.962997673885315 35.67988045012965 16.192342764847677 7 34.80830351882157 19.999988499706166 27.799851952884065 17.391856028588197 8 27.16303878944834 31.713224110233146 23.967330803876933 17.156406296441585 9 32.663812815577444 26.38631776554881 23.145954974615826 17.803914444257916 10-11 26.761325840251125 29.88213952889532 26.55332268586073 16.803211944992828 12-13 28.805541355160475 23.94226863722786 26.2451765778396 21.007013429772066 14-15 25.19060950773735 24.29812486429523 28.610848831065695 21.900416796901727 16-17 27.18497224136332 25.329823039170225 29.635468125665415 17.849736593801044 18-19 26.732857878035205 25.491837340286466 29.900630781682352 17.874673999995977 20-21 25.075067206239112 28.655404805316753 28.34988575490242 17.919642233541715 22-23 28.840377641969024 25.793964331472104 27.37505720839145 17.990600818167422 24-25 24.277083330528964 30.095857501385996 27.327214488466733 18.299844679618303 26-27 22.93410256745039 31.489841639715184 26.985532602526362 18.590523190308065 28-29 24.100082452065426 29.944474332771005 26.860155973825577 19.095287241337992 30-31 26.68334453464776 26.746090446999617 27.64558606159866 18.92497895675397 32-33 22.410321816741718 29.330460616312788 29.648846913886445 18.610370653059046 34-35 27.651969087053136 26.725206612562 27.827633085543223 17.79519121484164 36-37 23.47408052838124 27.18563775454867 29.925043225834564 19.415238491235527 38-39 20.37694408065504 29.859114110541146 31.192734378165955 18.57120743063786 40-41 22.0909438779797 30.6995819571817 28.098901431293726 19.110572733544874 42-43 24.87682802996898 27.108746588429916 29.884777552661383 18.12964782893972 44-45 22.078775671833778 29.504619211294592 30.138303637717716 18.278301479153917 46-47 24.123580343550856 30.194365074410406 27.340827998866672 18.34122658317206 48-49 20.923626096745124 29.168683114478117 29.78860455920971 20.11908622956705 50-51 19.869745575274365 26.989073359318134 31.409841526536823 21.73133953887067 52-53 25.945399933285945 26.567119997610355 27.371376865359494 20.11610320374421 54-55 27.335952863815145 26.623427129424783 27.322749585176993 18.71787042158309 56-57 27.89942393703307 26.176113751667813 26.915671307880846 19.00879100341827 58-59 25.743158522270964 26.035561826988165 27.358084429449605 20.86319522129127 60-61 21.50108093271932 26.589629670021512 26.516418666713005 25.392870730546164 62-63 24.822377791571316 26.746125127874258 27.065226314038544 21.366270766515882 64-65 21.663129109367986 28.45013129142972 29.965630631396127 19.921108967806163 66-67 20.52843740697708 28.22334143132868 28.233203061234285 23.01501810045995 68-69 20.035237734696207 26.483679817242294 33.99729001062739 19.483792437434108 70-71 19.65154131862878 31.455283951873575 30.16276701037658 18.730407719121068 72-73 19.541312653553927 35.603538205004256 26.393297321869763 18.461851819572047 74-75 20.41228964774709 35.26493651706663 25.70768898776726 18.615084847419023 76-77 20.905267239757507 34.201121865629524 26.162371044962928 18.73123984965004 78-79 20.514306942668554 33.88631625024479 26.97105397549466 18.628322831592005 80-81 19.761863481093677 34.307358870987095 26.680738623152468 19.250039024766757 82-83 19.064391761690974 33.80050143373096 27.152908287791732 19.982198516786333 84-85 19.28613700244413 32.83014838488943 27.586989005400554 20.296725607265884 86-87 19.301416930654433 32.571737587453946 28.174315270527288 19.952530211364326 88-89 19.729470781321318 32.928597775577366 27.70518895261878 19.636742490482533 90-91 20.321289714114357 31.858517376858963 27.483107665306463 20.33708524372022 92-93 19.94922671692728 32.4979829318206 26.892655106878692 20.660135244373425 94-95 19.626289253596084 32.81453755827081 26.842999055379344 20.71617413275376 96-97 20.389035658738536 32.922701966169946 26.646448005399666 20.041814369691856 98-99 20.827144453472343 31.78358117174973 26.655276343506568 20.73399803127135 100-101 20.798546404174527 31.79065915866181 26.18132503804408 21.229469399119587 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 931.0 1 846.5 2 568.5 3 345.0 4 306.0 5 332.5 6 466.5 7 662.5 8 925.5 9 1234.0 10 1489.0 11 1755.5 12 1897.5 13 1773.0 14 1626.5 15 1543.5 16 1332.5 17 1145.5 18 1093.5 19 1091.0 20 1151.0 21 1277.5 22 1447.5 23 1706.0 24 2081.0 25 2487.5 26 3081.0 27 3869.5 28 4802.5 29 5940.0 30 7709.0 31 11274.5 32 20567.5 33 38589.0 34 67835.0 35 109265.5 36 165077.0 37 241318.0 38 314204.5 39 342132.5 40 346801.0 41 330194.5 42 311594.0 43 336784.5 44 345535.5 45 332014.0 46 341992.0 47 319369.0 48 253227.5 49 204123.0 50 170499.0 51 141456.5 52 111714.5 53 81717.0 54 61244.5 55 46383.0 56 35028.5 57 25431.0 58 17324.5 59 12133.0 60 8869.0 61 6594.5 62 4715.0 63 3244.0 64 2337.5 65 1710.5 66 1254.5 67 939.5 68 745.5 69 565.0 70 410.5 71 301.5 72 235.0 73 193.5 74 166.0 75 143.0 76 122.5 77 102.5 78 80.0 79 65.5 80 60.0 81 46.5 82 31.5 83 30.5 84 31.5 85 24.5 86 18.0 87 11.5 88 8.5 89 11.5 90 10.5 91 8.5 92 5.0 93 3.0 94 3.0 95 2.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18618448706336513 2 0.19123771052226596 3 0.16208597064383426 4 0.16421062141632664 5 0.17119708566821606 6 0.16890016591417023 7 0.13647531538622323 8 0.11750658641739474 9 0.11821480334155884 10-11 0.09562842576010817 12-13 0.1415859618389752 14-15 0.11980350617144056 16-17 0.11163030004662747 18-19 0.11525751915822484 20-21 0.1065100830949003 22-23 0.10584014816663694 24-25 0.46261877946278107 26-27 0.3113091906650119 28-29 0.39733840595300346 30-31 0.26204026194072877 32-33 0.2899861189482864 34-35 0.30911754639969313 36-37 0.2893927480118246 38-39 0.3372835248836802 40-41 0.33277581986636523 42-43 0.37656085267786393 44-45 0.3296462667014778 46-47 0.35340024515790175 48-49 0.3492179371057433 50-51 0.36438717798142095 52-53 0.35580244040067466 54-55 0.3320580324432259 56-57 0.34644249240293795 58-59 0.3389775032022889 60-61 0.30921325138944505 62-63 0.31842007140357875 64-65 0.3196259542744528 66-67 0.33186662246372206 68-69 0.31809467443842226 70-71 0.30669621015896986 72-73 0.29297211462854594 74-75 0.30135587173081324 76-77 0.2910962968294086 78-79 0.3010496157636071 80-81 0.309261103884321 82-83 0.335321572593766 84-85 0.33348403679052935 86-87 0.32685168100072204 88-89 0.3402982320608653 90-91 0.32957927320865144 92-93 0.3126490605215386 94-95 0.30526063531269115 96-97 0.29044550289909554 98-99 0.31503211476636117 100-101 0.31798939894969513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5224388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.470213904491583 #Duplication Level Percentage of deduplicated Percentage of total 1 85.19043898776027 26.809613375623552 2 5.911155836883702 3.720506772190282 3 2.364514784244906 2.2323535812155986 4 1.0879396276109348 1.3695077118435612 5 0.8580723353387422 1.3501859969318426 6 0.7157345597936888 1.3514591817326707 7 0.4218767743885083 0.9293586631940304 8 0.3448573781989895 0.8682188366771482 9 0.2629960081954408 0.744888657054415 >10 2.2238899134032146 13.891127016460509 >50 0.27266072027785354 6.005211951473169 >100 0.2809305922434902 18.98958882616141 >500 0.04352669972197619 9.45135627891828 >1k 0.02097485969703883 10.791106202818893 >5k 3.693629915760118E-4 0.8360658979651695 >10k+ 6.15592496668817E-5 0.6594510497396283 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 34040 0.6515595702310012 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8822 0.16886188391826948 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 8539 0.16344498149831138 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7766 0.14864899008266613 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7720 0.14776850417694856 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 5270 0.10087305919851282 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004919236473248158 0.0 0.0 2 0.005570030403561145 0.0 0.0 3 0.006182542337973366 0.0 0.0 4 0.006546221299030624 0.0 0.0 5 0.007235297225244373 0.0 0.0 6 0.008230629118664234 0.0 0.0 7 0.009436511989538297 0.0 0.0 8 0.010776381846065032 0.0 0.0 9 0.012097110704641386 0.0 0.0 10-11 0.01846149252314338 0.0 0.0 12-13 0.0238496834461759 0.0 0.0 14-15 0.029113457882530933 0.0 0.0 16-17 0.03355416940701954 0.0 0.0 18-19 0.03874137985157304 0.0 0.0 20-21 0.04470380071311702 0.0 0.0 22-23 0.051192599018296495 0.0 0.0 24-25 0.057863236804004604 0.0 0.0 26-27 0.06569190496571081 0.0 0.0 28-29 0.07431492454235789 0.0 0.0 30-31 0.11667395300655312 0.0 0.0 32-33 0.12805327628805518 0.0 0.0 34-35 0.13792046073147707 0.0 0.0 36-37 0.14938591850375585 0.0 0.0 38-39 0.16144474721249646 0.0 0.0 40-41 0.17461375380235925 9.570498975190969E-6 0.0 42-43 0.18852925931228692 3.8281995900763876E-5 0.0 44-45 0.20379420517771651 3.8281995900763876E-5 0.0 46-47 0.2210880968258866 3.8281995900763876E-5 0.0 48-49 0.23861168044946127 3.8281995900763876E-5 0.0 50-51 0.2569966089808031 3.8281995900763876E-5 0.0 52-53 0.27638643990454004 3.8281995900763876E-5 0.0 54-55 0.2966184747380937 3.8281995900763876E-5 0.0 56-57 0.32020018421296426 3.8281995900763876E-5 0.0 58-59 0.3441360021499169 3.8281995900763876E-5 1.9140997950381938E-5 60-61 0.3693638374485203 3.8281995900763876E-5 1.9140997950381938E-5 62-63 0.39675460551551683 3.8281995900763876E-5 1.9140997950381938E-5 64-65 0.4273610612381776 3.8281995900763876E-5 1.9140997950381938E-5 66-67 0.4594892262978936 3.8281995900763876E-5 1.9140997950381938E-5 68-69 0.49464166903377005 3.8281995900763876E-5 1.9140997950381938E-5 70-71 0.5330097994253107 3.8281995900763876E-5 1.9140997950381938E-5 72-73 0.5736078560780709 3.8281995900763876E-5 1.9140997950381938E-5 74-75 0.6172589019039167 3.8281995900763876E-5 1.9140997950381938E-5 76-77 0.6649486982972934 3.8281995900763876E-5 1.9140997950381938E-5 78-79 0.7184095055727102 3.8281995900763876E-5 1.9140997950381938E-5 80-81 0.7758229289248808 3.8281995900763876E-5 1.9140997950381938E-5 82-83 0.8361170724685838 3.8281995900763876E-5 1.9140997950381938E-5 84-85 0.9036656542354817 3.8281995900763876E-5 1.9140997950381938E-5 86-87 0.9759324919971488 3.8281995900763876E-5 1.9140997950381938E-5 88-89 1.0543818720967892 3.8281995900763876E-5 1.9140997950381938E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 6590 0.0 90.511955 4 GAGCGGC 5360 0.0 89.58486 9 AGAGCGG 5530 0.0 89.57383 8 ATTCGGA 1185 0.0 89.23489 2 GAGAGCG 6345 0.0 87.25719 7 CGGAGAG 2300 0.0 85.342735 5 CGGAAAG 2435 0.0 85.0896 5 AAGCGGC 1840 0.0 84.49792 9 GTCCGGA 390 0.0 83.89433 2 TCGGGAG 7480 0.0 83.672554 3 GTTCGGA 910 0.0 83.37326 2 TCGGGAA 2255 0.0 81.79255 3 CCGGAGA 1215 0.0 80.780174 4 TTCGGAG 1010 0.0 78.398155 3 AAAGCGG 2105 0.0 78.13891 8 AAGGGGC 3885 0.0 78.087036 9 TTGGGAG 3905 0.0 77.46586 3 GATCCGA 245 0.0 77.425446 1 ACCGGAA 615 0.0 77.096664 3 TCGGAGA 1590 0.0 76.93665 4 >>END_MODULE