FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5224388
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT340400.6515595702310012Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC88220.16886188391826948No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG85390.16344498149831138No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC77660.14864899008266613No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG77200.14776850417694856No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA52700.10087305919851282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA65900.090.5119554
GAGCGGC53600.089.584869
AGAGCGG55300.089.573838
ATTCGGA11850.089.234892
GAGAGCG63450.087.257197
CGGAGAG23000.085.3427355
CGGAAAG24350.085.08965
AAGCGGC18400.084.497929
GTCCGGA3900.083.894332
TCGGGAG74800.083.6725543
GTTCGGA9100.083.373262
TCGGGAA22550.081.792553
CCGGAGA12150.080.7801744
TTCGGAG10100.078.3981553
AAAGCGG21050.078.138918
AAGGGGC38850.078.0870369
TTGGGAG39050.077.465863
GATCCGA2450.077.4254461
ACCGGAA6150.077.0966643
TCGGAGA15900.076.936654
Produced by FastQC (version 0.11.2)