##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2801229 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.868207133368962 33.0 33.0 33.0 22.0 33.0 2 30.253784321096205 33.0 33.0 33.0 22.0 33.0 3 30.092310910675277 33.0 33.0 33.0 15.0 33.0 4 33.34107243641987 37.0 37.0 37.0 15.0 37.0 5 33.49041724186063 37.0 37.0 37.0 15.0 37.0 6 34.204578775958694 37.0 37.0 37.0 27.0 37.0 7 33.3204172168716 37.0 37.0 37.0 15.0 37.0 8 33.87298003840457 37.0 37.0 37.0 22.0 37.0 9 33.082491292214954 37.0 37.0 37.0 15.0 37.0 10-11 33.14898603434421 37.0 37.0 37.0 15.0 37.0 12-13 33.11447511074603 37.0 37.0 37.0 15.0 37.0 14-15 34.59934657252228 40.0 37.0 40.0 15.0 40.0 16-17 34.54077977916121 40.0 37.0 40.0 15.0 40.0 18-19 34.61381040250547 40.0 37.0 40.0 15.0 40.0 20-21 34.6273408921584 40.0 37.0 40.0 15.0 40.0 22-23 35.5748908782538 40.0 37.0 40.0 24.5 40.0 24-25 34.71115696003433 40.0 37.0 40.0 15.0 40.0 26-27 34.65912140706811 38.5 37.0 40.0 18.5 40.0 28-29 34.20839370861861 37.0 37.0 40.0 15.0 40.0 30-31 34.17940357607321 37.0 37.0 40.0 15.0 40.0 32-33 34.06033976515308 37.0 37.0 40.0 15.0 40.0 34-35 34.20243917937448 37.0 37.0 40.0 15.0 40.0 36-37 34.03536001519333 37.0 37.0 40.0 15.0 40.0 38-39 33.86305011121904 37.0 35.0 40.0 15.0 40.0 40-41 33.849551036348686 37.0 33.0 40.0 15.0 40.0 42-43 33.722185333651765 37.0 33.0 40.0 15.0 40.0 44-45 33.503470797996165 37.0 33.0 40.0 15.0 40.0 46-47 33.45977729775038 37.0 33.0 40.0 10.5 40.0 48-49 33.3687793821926 37.0 33.0 40.0 10.5 40.0 50-51 32.45146041255463 37.0 33.0 38.5 15.0 38.5 52-53 32.89744858417502 37.0 33.0 38.5 15.0 40.0 54-55 33.45987778935603 37.0 33.0 40.0 10.5 40.0 56-57 33.80035298792066 37.0 33.0 40.0 18.5 40.0 58-59 33.1469599593607 37.0 33.0 40.0 6.0 40.0 60-61 33.05148293838169 37.0 33.0 40.0 10.5 40.0 62-63 32.594915124754166 37.0 33.0 40.0 6.0 40.0 64-65 32.482860915690935 37.0 33.0 40.0 6.0 40.0 66-67 32.425966781009336 37.0 33.0 37.0 10.5 40.0 68-69 32.63719710170072 37.0 33.0 37.0 15.0 40.0 70-71 32.135963714498175 37.0 33.0 37.0 15.0 40.0 72-73 32.56801889456378 37.0 33.0 37.0 18.5 40.0 74-75 32.72539945859478 37.0 33.0 37.0 22.0 37.0 76-77 32.3751267747121 37.0 33.0 37.0 22.0 37.0 78-79 31.823591716350215 37.0 33.0 37.0 18.5 37.0 80-81 31.23935672520883 37.0 33.0 37.0 15.0 37.0 82-83 30.816606389552586 37.0 33.0 37.0 15.0 37.0 84-85 30.578004154604997 33.0 27.0 37.0 15.0 37.0 86-87 30.37666949042724 33.0 27.0 37.0 6.0 37.0 88-89 30.221362123553625 33.0 27.0 37.0 6.0 37.0 90-91 30.066983456190123 33.0 27.0 37.0 6.0 37.0 92-93 29.931344242116587 33.0 27.0 37.0 6.0 37.0 94-95 29.79895074626173 33.0 27.0 37.0 6.0 37.0 96-97 29.64324569679951 33.0 27.0 37.0 6.0 37.0 98-99 29.50821835701401 33.0 27.0 37.0 6.0 37.0 100-101 28.38204516660366 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.013400757660321005 2306 2 -0.17580084377889094 2306 3 -0.205484275836465 2306 4 -0.008000924151957634 2306 5 -0.06717913623281646 2306 6 -0.210776526280668 2306 7 0.3650593287754802 2306 8 -0.21359073865846767 2306 9 -0.017694186106361087 2306 10-11 -0.0291254331249462 2306 12-13 -0.01328063827242687 2306 14-15 0.4676611557742021 2306 16-17 0.4190785038961735 2306 18-19 0.33443303243623745 2306 20-21 0.10950882310307719 2306 22-23 0.13872417025581996 2306 24-25 0.2979487741071054 2306 26-27 0.013091771240567596 2306 28-29 0.0676448620643626 2306 30-31 0.15295837997931727 2306 32-33 0.21692506138223422 2306 34-35 -0.0038627987042900713 2306 36-37 0.08408555950200736 2306 38-39 0.10707439897458926 2306 40-41 0.12256488469650861 2306 42-43 0.04558074778755383 2306 44-45 0.14080657174846323 2306 46-47 0.06510515044388399 2306 48-49 0.11604945285530732 2306 50-51 0.09584126593181708 2306 52-53 0.014039826066976957 2306 54-55 0.25362304876701103 2306 56-57 0.13975091388589078 2306 58-59 0.3621919353770551 2306 60-61 0.10136416158513839 2306 62-63 0.26405375222754657 2306 64-65 0.13315751070028625 2306 66-67 0.16143751219289726 2306 68-69 0.06419616815956175 2306 70-71 0.1938085837428858 2306 72-73 -0.05888935506635562 2306 74-75 0.05391044331167194 2306 76-77 0.14735683900380536 2306 78-79 0.17765840306941172 2306 80-81 0.3445047082677881 2306 82-83 0.19601198640075168 2306 84-85 0.08468070575618114 2306 86-87 0.11375472845067591 2306 88-89 0.17167643974411817 2306 90-91 0.2709584246875494 2306 92-93 0.09567229524826004 2306 94-95 0.19225704953340284 2306 96-97 0.17526918161306426 2306 98-99 0.1770999370516364 2306 100-101 0.03714263945039775 2307 1 0.004867444974497204 2307 2 0.016589254471963244 2307 3 -0.01054005891795029 2307 4 0.14206666113663857 2307 5 0.0424468918369314 2307 6 -0.18761326577804738 2307 7 0.4154402946062419 2307 8 -0.14993615664489113 2307 9 0.22459201237246162 2307 10-11 0.16318463876428524 2307 12-13 0.17298071840977514 2307 14-15 0.4732754421609755 2307 16-17 0.5016102286323942 2307 18-19 0.4476965612625392 2307 20-21 0.26538773447556707 2307 22-23 0.03874501745927006 2307 24-25 0.23603526982670076 2307 26-27 0.1108291771759724 2307 28-29 0.17360447228890763 2307 30-31 0.257443554927697 2307 32-33 0.2827739796813731 2307 34-35 0.18213359674698637 2307 36-37 0.28273183625872633 2307 38-39 0.13425747104539454 2307 40-41 0.2569113248683905 2307 42-43 0.07068414319175531 2307 44-45 0.18267885236527093 2307 46-47 0.09900606570336379 2307 48-49 0.12469129117516076 2307 50-51 0.07499708869023891 2307 52-53 -0.02309576817919634 2307 54-55 0.15136751585195896 2307 56-57 0.12705025955270344 2307 58-59 0.3348081249117598 2307 60-61 0.2563540406041085 2307 62-63 0.29066285371308 2307 64-65 0.1818071499091758 2307 66-67 0.24792953309389532 2307 68-69 0.029371251044011615 2307 70-71 0.18022932065790087 2307 72-73 -0.09067461542102251 2307 74-75 -0.16498788285963428 2307 76-77 0.13336399430202306 2307 78-79 0.10883236961973708 2307 80-81 0.3232257659607427 2307 82-83 0.3146312290420248 2307 84-85 0.192194105128074 2307 86-87 0.20038898107843295 2307 88-89 0.24901889204773298 2307 90-91 0.3121481275140283 2307 92-93 0.33406242039873035 2307 94-95 0.38240909962048875 2307 96-97 0.4368706503321711 2307 98-99 0.30255134625534197 2307 100-101 0.07946582490308529 2201 1 0.007246638591009713 2201 2 -0.16309121091712697 2201 3 -0.12254452703755803 2201 4 9.909893672670478E-4 2201 5 -0.023310736696572576 2201 6 -0.10391329440443542 2201 7 0.423070729867554 2201 8 -0.10610874777391643 2201 9 0.07008996148154267 2201 10-11 0.07900942922108811 2201 12-13 0.11374660455680186 2201 14-15 0.30666953151135345 2201 16-17 0.34784255483715043 2201 18-19 0.25568933059586385 2201 20-21 0.20355642600915758 2201 22-23 0.10959934514939818 2201 24-25 0.15332380986061622 2201 26-27 0.17325103012439058 2201 28-29 0.17827337701906742 2201 30-31 0.29168522574820344 2201 32-33 0.3111877772026901 2201 34-35 0.053098326227569714 2201 36-37 0.1678196872573423 2201 38-39 0.1574351408569541 2201 40-41 0.2936180684860048 2201 42-43 0.3082830093463471 2201 44-45 0.2857417763812151 2201 46-47 0.20774352163773102 2201 48-49 0.13122841840647226 2201 50-51 0.077755531974951 2201 52-53 0.041413509374706337 2201 54-55 0.3169401738426316 2201 56-57 0.3742549823070789 2201 58-59 0.425857072041012 2201 60-61 0.3292695687210383 2201 62-63 0.5235441702869608 2201 64-65 0.35583510368255133 2201 66-67 0.38931278845534223 2201 68-69 0.12354107562227057 2201 70-71 0.2976811112087887 2201 72-73 -0.03742551975481945 2201 74-75 0.13648227262371648 2201 76-77 0.3139670102827594 2201 78-79 0.20422231915321376 2201 80-81 0.236821701413227 2201 82-83 0.2999693107533652 2201 84-85 0.25825971762279565 2201 86-87 0.41877052886549393 2201 88-89 0.29328550586567204 2201 90-91 0.4927755660123232 2201 92-93 0.4129498546130783 2201 94-95 0.3341041624684351 2201 96-97 0.36570383452800215 2201 98-99 0.26824421508040075 2201 100-101 0.2659017187972559 1101 1 0.002458296512511282 1101 2 -0.22292650955180449 1101 3 -0.10101174784274392 1101 4 0.05175309943550843 1101 5 -0.006071238365940701 1101 6 0.12966557095559494 1101 7 0.42165595493063535 1101 8 -0.033514988222215436 1101 9 0.08240011696560146 1101 10-11 0.10418714244532623 1101 12-13 0.12827683455307692 1101 14-15 0.34522361539049484 1101 16-17 0.22723858382280326 1101 18-19 0.23864950695500653 1101 20-21 0.25709436264169483 1101 22-23 0.23052583937707993 1101 24-25 0.2050990535361592 1101 26-27 0.24479645171577147 1101 28-29 0.294509349597071 1101 30-31 0.46201406190879624 1101 32-33 0.525875106624305 1101 34-35 0.38461350141231065 1101 36-37 0.2662734277290255 1101 38-39 0.3089396410550691 1101 40-41 0.2781877438597036 1101 42-43 0.13832821943658047 1101 44-45 0.26033679338840443 1101 46-47 0.19949534341716202 1101 48-49 0.181008932100319 1101 50-51 0.12663851802284398 1101 52-53 0.287815010253631 1101 54-55 0.5149512745819038 1101 56-57 0.48895993224080314 1101 58-59 0.2694173616041482 1101 60-61 0.31980574018530206 1101 62-63 0.5609950927601943 1101 64-65 0.522965202652621 1101 66-67 0.4422435416763122 1101 68-69 0.3399938483409528 1101 70-71 0.46487694330676277 1101 72-73 0.2182779063071436 1101 74-75 0.366231931168997 1101 76-77 0.38327805559350736 1101 78-79 0.2956086940784708 1101 80-81 0.34685088985571255 1101 82-83 0.4849966977230942 1101 84-85 0.22437213575662796 1101 86-87 0.38685420741234466 1101 88-89 0.43467113819911063 1101 90-91 0.32082879646093687 1101 92-93 0.3316329331090664 1101 94-95 0.40356491795751737 1101 96-97 0.41262619481118534 1101 98-99 0.3424598501770113 1101 100-101 0.21340130212073305 2304 1 0.054443046320674426 2304 2 -0.11322330508430767 2304 3 -0.02829484958283146 2304 4 0.15163862714068443 2304 5 3.733147153539562E-4 2304 6 0.04096492988047373 2304 7 0.3178660592674163 2304 8 0.07433415858333348 2304 9 0.24750458239410733 2304 10-11 0.061994339619687366 2304 12-13 0.18926952981814082 2304 14-15 0.2529745349778665 2304 16-17 0.26103843337926236 2304 18-19 0.3039771656106254 2304 20-21 0.19259358847801877 2304 22-23 0.10507348714547504 2304 24-25 0.1806794744854514 2304 26-27 0.17848702538391592 2304 28-29 0.21766206492127793 2304 30-31 0.3264872396223595 2304 32-33 0.3853054997501175 2304 34-35 0.2213933639749257 2304 36-37 0.2466075516224535 2304 38-39 0.2079411742472459 2304 40-41 0.21460668319745224 2304 42-43 0.22667116612195315 2304 44-45 0.3201749278481927 2304 46-47 0.25791127691122995 2304 48-49 0.19415634507186041 2304 50-51 0.199180100626279 2304 52-53 0.1731058500988638 2304 54-55 0.33113243039387896 2304 56-57 0.40076177862998463 2304 58-59 0.37803840180365 2304 60-61 0.32961912865099663 2304 62-63 0.3735690343455218 2304 64-65 0.3478417627727737 2304 66-67 0.33254790479111307 2304 68-69 0.27302730361326155 2304 70-71 0.3288418317429489 2304 72-73 0.08126329734177773 2304 74-75 0.2392065308312823 2304 76-77 0.22992226167593088 2304 78-79 0.3002854599359601 2304 80-81 0.4203924023328902 2304 82-83 0.2648832243491306 2304 84-85 0.2514234676763394 2304 86-87 0.3009386888740373 2304 88-89 0.43100136529216826 2304 90-91 0.28659910823019175 2304 92-93 0.3414496008604182 2304 94-95 0.39510360419496493 2304 96-97 0.2832036366555748 2304 98-99 0.30704457405359165 2304 100-101 0.3899984025259009 2202 1 0.04381795827583446 2202 2 -0.10668676286056566 2202 3 -0.016034343209554436 2202 4 -0.09804394497212598 2202 5 -0.0591963081122131 2202 6 -0.10822477771269945 2202 7 0.12133504929164474 2202 8 0.0020412983551594266 2202 9 0.030074642763715076 2202 10-11 0.09078955035356273 2202 12-13 0.06606412855290955 2202 14-15 0.09024458051396067 2202 16-17 0.16657436640267775 2202 18-19 0.11768617869677911 2202 20-21 0.026775184284332454 2202 22-23 0.1785150118442047 2202 24-25 0.06863652632905115 2202 26-27 0.115026170469676 2202 28-29 0.16384486046973024 2202 30-31 0.20902212870218762 2202 32-33 0.002029401393649266 2202 34-35 0.10512356379823018 2202 36-37 0.10428837356709408 2202 38-39 -0.0027616731345503354 2202 40-41 0.1825562623081396 2202 42-43 -0.018416281645883714 2202 44-45 -0.008180781702257889 2202 46-47 0.1636862720833463 2202 48-49 0.07313655492411186 2202 50-51 0.12815158762550283 2202 52-53 0.12066727624334561 2202 54-55 0.33437877775747893 2202 56-57 0.3144545121668827 2202 58-59 0.2614070930118615 2202 60-61 0.29545446544175036 2202 62-63 0.29842911884990997 2202 64-65 0.2515689865638677 2202 66-67 0.2787085433599259 2202 68-69 0.29430943239989205 2202 70-71 0.3078262864060264 2202 72-73 0.30084876124362836 2202 74-75 0.23426785668628725 2202 76-77 0.40464030827928354 2202 78-79 0.25082455091205347 2202 80-81 0.20600659847300307 2202 82-83 0.29705026092073794 2202 84-85 0.3685557574392533 2202 86-87 0.4232258582569166 2202 88-89 0.4642627234263905 2202 90-91 0.3716142729335168 2202 92-93 0.1306578923287205 2202 94-95 0.3527345612738628 2202 96-97 0.3512584497579532 2202 98-99 0.2581711748370594 2202 100-101 0.33670713480148606 1102 1 0.030250603969417256 1102 2 0.012650148695236396 1102 3 0.19477490677122233 1102 4 0.14138644147880797 1102 5 0.16307965032468275 1102 6 0.19713747740905063 1102 7 0.40204065270676637 1102 8 0.3741011269313148 1102 9 0.29267394714333506 1102 10-11 0.3759828773894469 1102 12-13 0.33394733281775757 1102 14-15 0.34404698601972683 1102 16-17 0.42042378505035316 1102 18-19 0.35412695011859086 1102 20-21 0.32134436833062807 1102 22-23 0.28537261861409746 1102 24-25 0.23754734501067531 1102 26-27 0.3643682041363121 1102 28-29 0.2379948875313076 1102 30-31 0.2821863308064607 1102 32-33 0.18587951653231727 1102 34-35 0.15256845757610193 1102 36-37 0.21778970633162942 1102 38-39 0.23096046178446272 1102 40-41 0.22478893673197575 1102 42-43 0.17491452690760667 1102 44-45 0.20282029350570951 1102 46-47 0.09609414301195329 1102 48-49 0.12331328951316323 1102 50-51 0.1206878885908651 1102 52-53 0.09150411016943139 1102 54-55 0.3259106806724432 1102 56-57 0.44954634015101647 1102 58-59 0.4410500398072017 1102 60-61 0.5581586787769481 1102 62-63 0.4944134855292006 1102 64-65 0.43971877275564 1102 66-67 0.5433740317959987 1102 68-69 0.33890117985225743 1102 70-71 0.3064713212233272 1102 72-73 0.40372599454924796 1102 74-75 0.3792872205868676 1102 76-77 0.29803155193650355 1102 78-79 0.12133933030578348 1102 80-81 0.28554296763644516 1102 82-83 0.5004802613995771 1102 84-85 0.4768690303810139 1102 86-87 0.5611932508703426 1102 88-89 0.6447996454295577 1102 90-91 0.5330965798858003 1102 92-93 0.5142371014922205 1102 94-95 0.6519277199911002 1102 96-97 0.6353183102566113 1102 98-99 0.5264759738050948 1102 100-101 0.3839609288636119 2305 1 0.1946780499239935 2305 2 0.07461727750834513 2305 3 0.10737767107921314 2305 4 0.030628647193566394 2305 5 0.09534941743019942 2305 6 0.339415373256692 2305 7 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37944.0 31 48237.0 32 65806.0 33 94196.0 34 142456.0 35 242293.0 36 483481.0 37 864457.0 38 289430.0 39 439.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.08161442162099 22.031251899868607 23.540239306556003 13.346894371954399 2 30.410769107519403 27.12870054848373 30.953172879668333 11.50735746432853 3 35.93310510650089 19.321826648909067 30.368923013666794 14.376145230923246 4 27.09596799279194 17.479289348450234 35.00405810781338 20.420684550944447 5 35.58989916110016 16.034211065496834 31.834176667063574 16.541713106339433 6 31.38761766812324 16.385845964395013 37.478238079079844 14.748298288401902 7 35.48358385969904 19.671916681292075 28.666809882461774 16.177689576547113 8 26.950863307784157 33.31417770349015 24.284513995582056 15.450444993143641 9 34.266506511686586 26.899460817126336 22.472350214768973 16.361682456418105 10-11 26.97355292318494 30.809794896027714 26.599361668487454 15.617290512299897 12-13 29.35105936342216 23.733930103086482 26.577552751907014 20.337457781584344 14-15 25.109847665157474 24.23739211126775 29.286694677545793 21.36606554602898 16-17 27.38052120445197 25.163096393358963 30.41495124574357 17.041431156445494 18-19 27.18689251992852 25.32629592323748 30.85140618653818 16.63540537029582 20-21 25.217839076385186 29.14612513740014 29.016783185796434 16.61925260041824 22-23 29.54337418995066 25.86995935302901 27.888138906900405 16.69852755011992 24-25 23.895523241812793 31.351437325582626 27.867886510157025 16.885152922447556 26-27 22.74267000202671 32.80287334631479 27.71573909425352 16.73871755740498 28-29 24.421645565786555 30.887032649420735 27.601374097484648 17.089947687308058 30-31 27.719021499464517 26.927807272248284 28.156445257690038 17.196725970597164 32-33 27.121141298305773 26.700990306039422 29.20419080429851 16.9736775913563 34-35 22.384754058908975 26.69792678349907 28.24046618363716 22.676852973954798 36-37 19.097467320861103 26.95055750162507 29.601910380470265 24.350064797043558 38-39 19.215712473400266 29.260358648833034 31.583761594760603 19.9401672830061 40-41 21.76609432874485 26.729707477765185 33.6944407181695 17.809757475320467 42-43 23.28419304646844 26.349539271736145 32.89994703613798 17.46632064565743 44-45 20.868845415634862 29.222304426323724 32.468847063298774 17.440003094742647 46-47 23.94925979838772 30.54226875260417 28.094664694276545 17.41380675473157 48-49 19.911378956971486 29.22337736919422 31.243189834499656 19.622053839334637 50-51 19.170824796715632 27.020410488591928 32.72817402732721 21.080590687365234 52-53 26.208453937786913 26.441655389948725 27.806254700125287 19.54363597213907 54-55 27.55191241759106 26.661391682443565 27.943793120802095 17.842902779163285 56-57 28.39285563950259 26.4221970773201 27.412944832056873 17.772002451120443 58-59 25.78839563302165 26.41334481067579 27.7764762480983 20.02178330820426 60-61 20.84395001971436 26.63585305873375 27.353412732718063 25.16678418883383 62-63 24.722485211656902 26.6845764113191 27.811396850848013 20.78154152617599 64-65 20.5568635189945 29.0119263940615 30.884398376911587 19.546811710032415 66-67 19.156252882353414 28.90504151036662 28.878141037638272 23.060564569641688 68-69 18.800216894723683 27.158095337694625 35.36327717326292 18.678410594318763 70-71 18.330442218771665 32.7393298534914 30.885035581479297 18.04519234625764 72-73 18.2375592379941 36.43363778169768 27.027330890084038 18.30147209022418 74-75 18.65084082752812 36.64424913503731 26.493622802500603 18.211287234933966 76-77 18.868672713139603 36.2085886363498 26.685035713480104 18.237702937030488 78-79 19.13932386316841 35.723547337098005 26.91106591277756 18.226062886956022 80-81 18.65658680007807 35.80722792328367 26.807974602174145 18.72821067446412 82-83 18.495858256719128 34.99305157168451 27.096380853036667 19.41470931855969 84-85 18.769619446981217 33.865419929373125 27.650562996525252 19.714397627120405 86-87 18.91849926976449 33.50179044255585 27.95210801217447 19.627602275505186 88-89 19.376889746876685 33.785311321384995 27.413050374085028 19.4247485576533 90-91 19.85984559697792 33.097629367199055 27.070853926760734 19.97167110906229 92-93 19.596943005459686 33.2019296024582 27.310876217864582 19.890251174217532 94-95 19.241433087347282 33.11787545400586 27.41721806873942 20.22347338990744 96-97 19.58142687376675 33.64229943102498 26.993766732058273 19.782506963149995 98-99 20.010321512241216 32.88424130349024 26.9058222891302 20.199614895138343 100-101 20.234595598189763 32.7291696214065 26.357479109094008 20.678755671309727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 611.0 1 537.5 2 364.5 3 293.0 4 307.5 5 314.5 6 392.0 7 519.0 8 689.5 9 911.0 10 1103.0 11 1252.5 12 1265.5 13 1177.5 14 1106.0 15 1056.5 16 994.0 17 961.0 18 993.5 19 1048.0 20 1204.0 21 1451.5 22 1862.5 23 2522.0 24 3520.0 25 5083.5 26 7654.5 27 11462.0 28 15794.5 29 19914.5 30 24397.0 31 29075.0 32 35555.5 33 46561.0 34 62759.0 35 85299.5 36 110232.5 37 137723.5 38 168907.5 39 184580.0 40 180506.5 41 166569.0 42 153383.5 43 150982.5 44 145547.0 45 138400.5 46 140292.0 47 132710.0 48 110252.5 49 90966.5 50 78204.5 51 67296.0 52 57871.0 53 48806.0 54 40402.0 55 32511.5 56 24748.0 57 18544.5 58 13707.5 59 10239.0 60 7673.0 61 5571.5 62 4000.5 63 2846.5 64 2043.5 65 1455.0 66 1066.5 67 791.0 68 554.5 69 406.5 70 317.0 71 250.5 72 202.0 73 150.0 74 119.0 75 109.0 76 86.5 77 63.5 78 54.0 79 58.0 80 47.5 81 40.5 82 44.5 83 34.0 84 27.0 85 22.0 86 17.5 87 15.0 88 12.5 89 12.5 90 12.0 91 9.5 92 6.0 93 4.0 94 6.0 95 4.5 96 2.0 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17788620637584432 2 0.1840977656592874 3 0.15364684572378767 4 0.15561026963522082 5 0.1638566500632401 6 0.16014399393980283 7 0.12801523902544204 8 0.11052291690540116 9 0.1108799030711163 10-11 0.09019255476792508 12-13 0.13292379880402494 14-15 0.11243279289197705 16-17 0.10568575435996128 18-19 0.10854164368568225 20-21 0.1011341807470935 22-23 0.10229438578566764 24-25 0.45831668885335686 26-27 0.30440210350528285 28-29 0.39909268396121844 30-31 0.26720414503776735 32-33 0.29645916131812144 34-35 0.31603985250759575 36-37 0.29549529867069063 38-39 0.3431529517936591 40-41 0.33886911780507767 42-43 0.38008317063688835 44-45 0.33547774923078405 46-47 0.35805712421226543 48-49 0.35361264644911217 50-51 0.3680884354688603 52-53 0.359842055040841 54-55 0.3368342966605015 56-57 0.35172061977082203 58-59 0.34420606098251877 60-61 0.3158613594247382 62-63 0.3241612877776147 64-65 0.3264459992381915 66-67 0.3375839676085033 68-69 0.3245361232516156 70-71 0.3128983742493027 72-73 0.2985653796958406 74-75 0.307829170696148 76-77 0.2965127092429787 78-79 0.3069724038984317 80-81 0.3164325372898824 82-83 0.34236758222908587 84-85 0.33969018598622247 86-87 0.33276465437134917 88-89 0.345723252186808 90-91 0.3348351741324968 92-93 0.31928842661560336 94-95 0.3115239775112995 96-97 0.2967269009424078 98-99 0.3213767956850368 100-101 0.3250359038836168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2801229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.60656220109777 #Duplication Level Percentage of deduplicated Percentage of total 1 92.33393569791153 56.883763528455475 2 3.5841999260137314 4.416204713662699 3 1.1809747170236347 2.1826737708672117 4 0.6242635109414388 1.538349152667577 5 0.36479924348589693 1.123701364236366 6 0.2950559306104531 1.09064289251734 7 0.20209992970477592 0.8715477323136335 8 0.1794739355297986 0.8845417738153878 9 0.14085898799441457 0.7810054204915425 >10 0.9241394726933324 10.876631919143996 >50 0.08290821675978981 3.455958867902045 >100 0.07702395357183742 9.964813649123423 >500 0.008012612959326962 3.268045633130906 >1k 0.0021956460527888717 2.1384676783178813 >5k 0.0 0.0 >10k+ 5.821874722671547E-5 0.5236519033547246 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 14600 0.5211998019440752 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3501 0.12498085661686352 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3325 0.11869790010027742 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTGCCTCTATGTGTAGATCT 2844 0.10152686552938013 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005104902169726216 0.0 0.0 2 0.005497586952012848 0.0 0.0 3 0.006211559283443089 0.0 0.0 4 0.006711339915444257 0.0 0.0 5 0.007960791495447176 0.0 3.5698616571512E-5 6 0.008781859676591954 0.0 3.5698616571512E-5 7 0.010102708489737898 0.0 3.5698616571512E-5 8 0.01103087252059721 0.0 3.5698616571512E-5 9 0.012173228250885593 0.0 3.5698616571512E-5 10-11 0.01838478753432868 0.0 5.354792485726801E-5 12-13 0.023864525178055776 0.0 7.1397233143024E-5 14-15 0.029094372505782284 0.0 7.1397233143024E-5 16-17 0.032735631396076506 0.0 7.1397233143024E-5 18-19 0.03723365708408702 0.0 7.1397233143024E-5 20-21 0.04264199749467108 0.0 7.1397233143024E-5 22-23 0.047639803814682774 0.0 7.1397233143024E-5 24-25 0.05404770548926917 0.0 7.1397233143024E-5 26-27 0.060741196096427676 0.0 7.1397233143024E-5 28-29 0.068398549351017 0.0 7.1397233143024E-5 30-31 0.1348515240988866 0.0 7.1397233143024E-5 32-33 0.14613228693548438 0.0 7.1397233143024E-5 34-35 0.1543965166717894 0.0 7.1397233143024E-5 36-37 0.1644635265449558 0.0 7.1397233143024E-5 38-39 0.1748161253506943 0.0 1.0709584971453601E-4 40-41 0.18622183334529235 0.0 1.0709584971453601E-4 42-43 0.19812732197189162 0.0 1.0709584971453601E-4 44-45 0.21101452255420744 0.0 1.0709584971453601E-4 46-47 0.22545461295738406 0.0 1.0709584971453601E-4 48-49 0.24135834663999267 0.0 1.0709584971453601E-4 50-51 0.256405313524885 0.0 1.0709584971453601E-4 52-53 0.27370129325378256 3.5698616571512E-5 1.0709584971453601E-4 54-55 0.2935854226841147 3.5698616571512E-5 1.0709584971453601E-4 56-57 0.31589705804130974 3.5698616571512E-5 1.0709584971453601E-4 58-59 0.3397080352945082 3.5698616571512E-5 1.0709584971453601E-4 60-61 0.3654467378425684 3.5698616571512E-5 1.0709584971453601E-4 62-63 0.3913817827817719 3.5698616571512E-5 1.24945158000292E-4 64-65 0.41853058068440674 3.5698616571512E-5 1.42794466286048E-4 66-67 0.44826752828847627 3.5698616571512E-5 1.7849308285756003E-4 68-69 0.4818420771739833 3.5698616571512E-5 1.7849308285756003E-4 70-71 0.5180761729940679 3.5698616571512E-5 1.7849308285756003E-4 72-73 0.5565771309664437 3.5698616571512E-5 1.9634239114331604E-4 74-75 0.5998617035594019 3.5698616571512E-5 2.1419169942907203E-4 76-77 0.6458058230869379 3.5698616571512E-5 2.1419169942907203E-4 78-79 0.6969262420173431 3.5698616571512E-5 2.1419169942907203E-4 80-81 0.7523304949363296 3.5698616571512E-5 2.1419169942907203E-4 82-83 0.812375568009613 3.5698616571512E-5 2.1419169942907203E-4 84-85 0.8772221050117646 3.5698616571512E-5 2.1419169942907203E-4 86-87 0.9476733248156435 3.5698616571512E-5 2.1419169942907203E-4 88-89 1.0261745826563984 3.5698616571512E-5 2.1419169942907203E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3630 0.0 87.92583 9 CGGGAGA 4475 0.0 87.784164 4 GAGAGCG 4185 0.0 85.560844 7 GATCGGG 5635 0.0 83.62183 1 AAGCGGC 1040 0.0 82.081856 9 TCGGGAG 4950 0.0 81.085655 3 ATCGGGA 5695 0.0 80.733826 2 ATTCGGA 720 0.0 79.740395 2 CGGAAAG 1615 0.0 79.61005 5 ATCCGGA 735 0.0 79.40416 2 GTTCGGA 625 0.0 77.436485 2 GATCCGG 790 0.0 76.885994 1 AGAGCGG 4360 0.0 76.46749 8 TCGGGAA 1450 0.0 72.638 3 AAGCGTC 435 0.0 69.77482 9 GGTCCGG 430 0.0 68.42071 1 AAAGCGG 1360 0.0 68.3479 8 TTCGGAG 835 0.0 67.614426 3 CCGGAAA 910 0.0 67.2554 4 TCGGAGA 1145 0.0 67.12564 4 >>END_MODULE