FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801229
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT146000.5211998019440752Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC35010.12498085661686352No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33250.11869790010027742No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTGCCTCTATGTGTAGATCT28440.10152686552938013Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC36300.087.925839
CGGGAGA44750.087.7841644
GAGAGCG41850.085.5608447
GATCGGG56350.083.621831
AAGCGGC10400.082.0818569
TCGGGAG49500.081.0856553
ATCGGGA56950.080.7338262
ATTCGGA7200.079.7403952
CGGAAAG16150.079.610055
ATCCGGA7350.079.404162
GTTCGGA6250.077.4364852
GATCCGG7900.076.8859941
AGAGCGG43600.076.467498
TCGGGAA14500.072.6383
AAGCGTC4350.069.774829
GGTCCGG4300.068.420711
AAAGCGG13600.068.34798
TTCGGAG8350.067.6144263
CCGGAAA9100.067.25544
TCGGAGA11450.067.125644
Produced by FastQC (version 0.11.2)