FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4775649
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT369960.7746800487221737Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTTCAGAGCCGTGTAGATCT80900.16940105941621758Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGGAGATCT73460.1538220250273837Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC173750.090.157069
ATCGGGA267900.089.013812
TCGGGAG248100.087.412353
GAGAGCG200900.086.823827
GATCGGG286100.084.911331
AGAGCGG191100.084.080528
GAGCGTC369050.083.0168469
CGGGAGA250100.081.593594
TCCGGAG34250.081.1400763
ATTGGGA104050.080.543272
AGAGCGT386750.079.891758
GGAGAGC223450.079.2387546
AAGAGCG384200.078.79397
CGGAAGA400600.078.736184
TCGGAAG415800.078.093383
GAAGAGC391550.077.796856
GGATCGG48500.077.548851
GATCGGA440400.076.915961
GAGGGGC187700.076.9143759
CGGAGAG80450.076.0426945
Produced by FastQC (version 0.11.2)