FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043743
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA44120.14495310543629997No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40180.13200851714484435No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT34900.11466145466289368Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10900.067.424379
AGAGCGG13700.055.720768
GATCGGG24250.047.5287441
ATCGGGA23400.046.0090452
CGGGAGA21000.044.9353754
TTGTGTT260850.043.8033941
TCGGGAG21950.043.638663
GGATCGG5750.042.0691871
TCGGGAA6350.041.8185423
GAGAGCG20900.039.702497
GGCGCCG12600.039.0179756-57
CGCCGGA8850.035.69101758-59
TCTCGGG12000.033.45490648-49
TCGGAAA7550.033.2876784
GCCGGAT11050.033.0985558-59
GATCTCG20900.032.16660746-47
GTCGCCG12350.031.92316856-57
ATCTCGG21250.031.74859446-47
CCTATGT13900.031.44956436-37
TGTGTTT366950.031.2781942
Produced by FastQC (version 0.11.2)