FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_30484-1.34200000035803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_30484-1.34200000035803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8315787
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT195840.23550386752330238Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC45800.082.2933969
CGGGAGA69150.079.001164
AGAGCGG51650.075.450968
ATTCGGA9400.074.671822
GATCGGG87750.074.269791
ATCGGGA88700.071.6479952
GATCCGG12400.071.148191
TTCGGAG11250.067.8651053
GAGAGCG70900.067.804777
AAGCGGC13750.065.855799
CGGAAAG18650.064.8375555
TCGGGAA18150.062.1832583
GGACCGG8200.059.5793721
AAAGCGG17700.053.837658
GAACCGG6000.053.7564051
GTTCGGA10000.053.1178972
TCGGGAG112000.051.909353
AACCGGA6100.051.3141252
ATCCGGA16200.050.9397852
AAGGGGC40850.050.4847839
Produced by FastQC (version 0.11.2)