##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_30423-1.34200000035748.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2567359 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.05706915160677 33.0 33.0 33.0 27.0 33.0 2 30.504421080183956 33.0 33.0 33.0 22.0 33.0 3 30.365475182863012 33.0 33.0 33.0 22.0 33.0 4 33.671026919102474 37.0 37.0 37.0 22.0 37.0 5 33.80395418015166 37.0 37.0 37.0 22.0 37.0 6 34.461931891877995 37.0 37.0 37.0 27.0 37.0 7 33.67667163026285 37.0 37.0 37.0 22.0 37.0 8 34.089763060016146 37.0 37.0 37.0 27.0 37.0 9 33.4335131939086 37.0 37.0 37.0 15.0 37.0 10-11 33.43733385163509 37.0 37.0 37.0 15.0 37.0 12-13 33.40735382157307 37.0 37.0 37.0 18.5 37.0 14-15 34.845503492109984 40.0 37.0 40.0 15.0 40.0 16-17 34.73654560971021 40.0 37.0 40.0 15.0 40.0 18-19 34.87056309616224 40.0 37.0 40.0 18.5 40.0 20-21 34.873733474749734 40.0 37.0 40.0 15.0 40.0 22-23 35.72160282219978 40.0 37.0 40.0 24.5 40.0 24-25 34.8921481179687 40.0 37.0 40.0 18.5 40.0 26-27 34.79061031199766 37.0 37.0 40.0 18.5 40.0 28-29 34.449429939482556 37.0 37.0 40.0 15.0 40.0 30-31 34.46704473351798 37.0 37.0 40.0 15.0 40.0 32-33 34.276489380721586 37.0 37.0 40.0 15.0 40.0 34-35 34.63210423629886 37.0 37.0 40.0 18.5 40.0 36-37 34.10304090701768 37.0 37.0 40.0 15.0 40.0 38-39 34.0522342220157 37.0 35.0 40.0 15.0 40.0 40-41 34.63897102041437 37.0 33.0 40.0 18.5 40.0 42-43 34.1677096970077 37.0 33.0 40.0 15.0 40.0 44-45 33.613235624624366 37.0 33.0 40.0 15.0 40.0 46-47 33.48369141206976 37.0 33.0 40.0 10.5 40.0 48-49 33.38780883390285 37.0 33.0 40.0 15.0 40.0 50-51 32.396915468386 37.0 33.0 38.5 15.0 38.5 52-53 32.904207787068344 37.0 33.0 38.5 15.0 40.0 54-55 33.535521132806124 37.0 33.0 40.0 15.0 40.0 56-57 33.815751322662706 37.0 33.0 40.0 18.5 40.0 58-59 33.21280506543884 37.0 33.0 40.0 15.0 40.0 60-61 33.056664455574776 37.0 33.0 40.0 15.0 40.0 62-63 32.649649114128565 37.0 33.0 38.5 6.0 40.0 64-65 32.45011196330548 37.0 33.0 37.0 6.0 40.0 66-67 32.30168648015334 37.0 33.0 37.0 10.5 40.0 68-69 32.44641205222955 37.0 33.0 37.0 15.0 40.0 70-71 32.00612633449393 37.0 33.0 37.0 15.0 37.0 72-73 32.313299386645966 37.0 33.0 37.0 18.5 37.0 74-75 32.440519810435546 37.0 33.0 37.0 22.0 37.0 76-77 32.10075022620522 37.0 33.0 37.0 22.0 37.0 78-79 31.67483764444318 35.0 33.0 37.0 18.5 37.0 80-81 31.213891590541095 33.0 33.0 37.0 15.0 37.0 82-83 30.704516392136824 33.0 30.0 37.0 15.0 37.0 84-85 30.509159022949262 33.0 27.0 37.0 15.0 37.0 86-87 30.35811275322228 33.0 27.0 37.0 15.0 37.0 88-89 30.278715987908196 33.0 27.0 37.0 15.0 37.0 90-91 30.182956298671122 33.0 27.0 37.0 6.0 37.0 92-93 30.013186702755632 33.0 27.0 37.0 6.0 37.0 94-95 29.815508660845637 33.0 27.0 37.0 6.0 37.0 96-97 29.652694656259605 33.0 27.0 37.0 6.0 37.0 98-99 29.53472887897641 33.0 27.0 37.0 6.0 37.0 100-101 28.299961945329812 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 -0.09460424894434993 2306 2 -0.13340735177017748 2306 3 -0.2910765774587496 2306 4 -0.13580182054894152 2306 5 -0.2626304823316872 2306 6 -0.32044845715126513 2306 7 0.30565308059310325 2306 8 -0.3881618141070575 2306 9 -0.10343063921552442 2306 10-11 -0.12938652112970317 2306 12-13 -0.02940084454974823 2306 14-15 0.23006908607623444 2306 16-17 0.17777185697408981 2306 18-19 0.16934995450299795 2306 20-21 0.11006402004576188 2306 22-23 0.08690218662092519 2306 24-25 0.3271403079114421 2306 26-27 0.013598514867616984 2306 28-29 0.08468632440796853 2306 30-31 0.13318041519855228 2306 32-33 0.025040447154914602 2306 34-35 -0.1491800846433975 2306 36-37 0.2655949766971588 2306 38-39 0.05691608788882263 2306 40-41 -0.15132367812530845 2306 42-43 0.16079312761587516 2306 44-45 0.28873405548232967 2306 46-47 0.01408154434093234 2306 48-49 0.053297356136390306 2306 50-51 0.13596153130917799 2306 52-53 -0.005988311720656725 2306 54-55 0.03451997647416505 2306 56-57 0.10001469287902864 2306 58-59 0.35632758606642767 2306 60-61 0.1686543749327427 2306 62-63 0.23927506020416445 2306 64-65 0.21682261942391534 2306 66-67 0.034063579788472964 2306 68-69 -3.981203699794378E-4 2306 70-71 0.21800959352091098 2306 72-73 -0.16056433322979302 2306 74-75 -0.11834705991164896 2306 76-77 0.09274460784951088 2306 78-79 0.08937560563416014 2306 80-81 0.21210799350271614 2306 82-83 0.25446485453501566 2306 84-85 0.17774542947811156 2306 86-87 0.28687987238269486 2306 88-89 0.32935437391929767 2306 90-91 0.38989168077886305 2306 92-93 0.19598157970679964 2306 94-95 0.4536510632203985 2306 96-97 0.3529352847228253 2306 98-99 0.38796752513446364 2306 100-101 0.19697306129948444 2307 1 0.05035134060361557 2307 2 -0.030684811655987687 2307 3 -0.032082752746269705 2307 4 0.13748581187982012 2307 5 0.06351597458923663 2307 6 -0.02917821120989572 2307 7 0.4628106857649854 2307 8 0.011190441666926176 2307 9 0.14114222099384222 2307 10-11 0.1432719369998381 2307 12-13 0.088267024440988 2307 14-15 0.43960301238825394 2307 16-17 0.369282567730167 2307 18-19 0.31121947401400263 2307 20-21 0.28502672281991437 2307 22-23 0.11604469664553818 2307 24-25 0.3403671316985779 2307 26-27 0.08558877037722112 2307 28-29 0.28783754656094374 2307 30-31 0.2389865073935198 2307 32-33 0.2860775933033892 2307 34-35 0.03770388164117122 2307 36-37 0.1815150693579497 2307 38-39 0.07365577528456413 2307 40-41 -0.016144368171197243 2307 42-43 0.20814864414310819 2307 44-45 0.3096154660413859 2307 46-47 0.14492587799530554 2307 48-49 0.11873651916774719 2307 50-51 0.09786107744758255 2307 52-53 0.23637881887943735 2307 54-55 0.25523740513731497 2307 56-57 0.3578392416927585 2307 58-59 0.35863623713436255 2307 60-61 0.24751739182193688 2307 62-63 0.3023624521681043 2307 64-65 0.265186577399227 2307 66-67 0.20701978623569772 2307 68-69 0.14367996145100648 2307 70-71 0.13478264383597605 2307 72-73 -0.0032553359428888484 2307 74-75 0.1396653913816337 2307 76-77 0.17157961247625053 2307 78-79 0.3926341556691 2307 80-81 0.4263369638474863 2307 82-83 0.402154314999958 2307 84-85 0.2892480827744315 2307 86-87 0.370822865854894 2307 88-89 0.4011671135185644 2307 90-91 0.4067475089031731 2307 92-93 0.3324695973828504 2307 94-95 0.2876289933345255 2307 96-97 0.34915362929253746 2307 98-99 0.3038712510801176 2307 100-101 0.010975415588507786 2201 1 0.07397548570745371 2201 2 -0.15145544038704273 2201 3 0.044977019078427105 2201 4 0.025078772762519463 2201 5 -0.057418176204045324 2201 6 0.020227442480823754 2201 7 0.3323147233502439 2201 8 0.010576388129805991 2201 9 0.18082338680464716 2201 10-11 0.15282853283137854 2201 12-13 0.15690992101441026 2201 14-15 0.43024461657351054 2201 16-17 0.35470598555554034 2201 18-19 0.327408581076412 2201 20-21 0.21291983690587557 2201 22-23 0.2589383097557274 2201 24-25 0.1798844653191054 2201 26-27 0.04919030996563123 2201 28-29 -0.048478983658782226 2201 30-31 0.20859649599772467 2201 32-33 0.07787834336217259 2201 34-35 -0.03667300062463852 2201 36-37 0.02651631268948762 2201 38-39 -0.06120552135516988 2201 40-41 -0.05815558240145435 2201 42-43 0.18832920633196437 2201 44-45 0.058348592961003476 2201 46-47 -0.014298796243373602 2201 48-49 -0.11115266362228482 2201 50-51 -0.02122893919219848 2201 52-53 -4.905004909900867E-4 2201 54-55 0.133882185033805 2201 56-57 0.228272958588704 2201 58-59 0.3475395736046849 2201 60-61 0.20907800691298917 2201 62-63 0.2842153093778208 2201 64-65 0.1744971007205791 2201 66-67 0.15207383591596368 2201 68-69 0.028199412400311985 2201 70-71 0.03509226440276336 2201 72-73 -0.12001939221477897 2201 74-75 0.10693729643057992 2201 76-77 0.2437863032351686 2201 78-79 0.1496092174268675 2201 80-81 0.22195271181150034 2201 82-83 0.24769038824882017 2201 84-85 0.18580191169990812 2201 86-87 0.2562435207212843 2201 88-89 0.2170810242276282 2201 90-91 0.3350175695360278 2201 92-93 0.13316693439787386 2201 94-95 0.13370736776734304 2201 96-97 0.02393277583141895 2201 98-99 0.13334353719184833 2201 100-101 0.19482059177225253 1101 1 -0.07937589957239055 1101 2 -0.463410963116484 1101 3 -0.20826446154363865 1101 4 -0.2710570577597693 1101 5 -0.2677473655273914 1101 6 -0.07969092415599732 1101 7 0.29153908950917895 1101 8 -0.2055427079607881 1101 9 -0.09130440597110834 1101 10-11 -0.09809375019527522 1101 12-13 -0.1508610423544141 1101 14-15 0.0767592416676095 1101 16-17 -0.10051241970965918 1101 18-19 -0.13458732339162083 1101 20-21 -0.08252103626393392 1101 22-23 0.04087542624134244 1101 24-25 -0.0968352381150126 1101 26-27 -0.17097117349443636 1101 28-29 -0.159662373838259 1101 30-31 -0.059827077586341204 1101 32-33 -0.1459839612974534 1101 34-35 -0.3492635401252002 1101 36-37 -0.3505228563736651 1101 38-39 -0.20395816139117784 1101 40-41 -0.31345461522415263 1101 42-43 -0.008653213544064897 1101 44-45 -0.12574198065541253 1101 46-47 -0.2764568800802678 1101 48-49 -0.35327863235585966 1101 50-51 -0.19544413213155565 1101 52-53 -0.22276893305429724 1101 54-55 -0.027122973661946048 1101 56-57 0.08881800196475353 1101 58-59 -0.040314317082724926 1101 60-61 -0.06855630008553959 1101 62-63 -0.030928753256141306 1101 64-65 -0.06320875983724505 1101 66-67 -0.2400180305684998 1101 68-69 -0.16980349247521076 1101 70-71 -0.10201046614216835 1101 72-73 -0.23809948316135632 1101 74-75 0.0025277356224151504 1101 76-77 -0.0889631644353841 1101 78-79 -0.19926413070721338 1101 80-81 -0.007754411168360065 1101 82-83 0.036787617852237275 1101 84-85 -0.02344494916699702 1101 86-87 -0.02045389188439728 1101 88-89 0.05942383922761607 1101 90-91 -0.14123766914845248 1101 92-93 -0.1282215721791431 1101 94-95 -0.07788084560372965 1101 96-97 0.050549520103697176 1101 98-99 -0.14778662412035004 1101 100-101 -0.044792765653301814 2304 1 0.07972994366907571 2304 2 -0.1783307303840722 2304 3 -0.15301262439322372 2304 4 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2304 64-65 0.21453219352807196 2304 66-67 0.14232438611104925 2304 68-69 0.040392498123132725 2304 70-71 0.11156485816032813 2304 72-73 -0.01122589808927188 2304 74-75 0.1231952266353602 2304 76-77 0.3586193361257557 2304 78-79 0.2135305346425369 2304 80-81 0.19147126426214456 2304 82-83 0.1990869463837086 2304 84-85 0.22046848539057962 2304 86-87 0.2412394393486288 2304 88-89 0.3757377205862902 2304 90-91 0.1452717438645692 2304 92-93 0.22282707899163157 2304 94-95 0.24959386048686483 2304 96-97 0.17094905475361344 2304 98-99 0.045025069963834596 2304 100-101 0.040166970377413236 2202 1 0.31055902865425367 2202 2 0.216785165594505 2202 3 0.25776946202065076 2202 4 0.29254054775774563 2202 5 0.20779732556267305 2202 6 0.321383088248524 2202 7 0.5590455796591058 2202 8 0.3465919542773932 2202 9 0.37897441731977466 2202 10-11 0.4526335006762281 2202 12-13 0.47903408458098085 2202 14-15 0.4421187669929978 2202 16-17 0.542964717439034 2202 18-19 0.49616819523738087 2202 20-21 0.44138792955878614 2202 22-23 0.33003751298551265 2202 24-25 0.5197213367547349 2202 26-27 0.47964417007473514 2202 28-29 0.4949042311914411 2202 30-31 0.6042970921582267 2202 32-33 0.5210913632325926 2202 34-35 0.4355397549381692 2202 36-37 0.48618709149088346 2202 38-39 0.4600398250892752 2202 40-41 0.4302358268953981 2202 42-43 0.5922992614859766 2202 44-45 0.6875659897290731 2202 46-47 0.6459932989556378 2202 48-49 0.629296349708973 2202 50-51 0.6387650416059998 2202 52-53 0.5147547671847832 2202 54-55 0.6003769265416423 2202 56-57 0.7124333971799786 2202 58-59 0.7476162706200569 2202 60-61 0.6714491171119619 2202 62-63 0.7463867660223826 2202 64-65 0.680407483365606 2202 66-67 0.6174289358121712 2202 68-69 0.6359731751003324 2202 70-71 0.6294664734434505 2202 72-73 0.6366127141256683 2202 74-75 0.5706653450686403 2202 76-77 0.7434090786745458 2202 78-79 0.7752467371787901 2202 80-81 0.7043737513349306 2202 82-83 0.766508817114218 2202 84-85 0.6692697095394138 2202 86-87 0.8023636056184351 2202 88-89 0.706995674040531 2202 90-91 0.8049198531219304 2202 92-93 0.7258393074681848 2202 94-95 0.7776459700877432 2202 96-97 0.7123499556896782 2202 98-99 0.79324440703936 2202 100-101 0.6742716423893214 1102 1 0.1266355171375224 1102 2 -0.01780501258889089 1102 3 0.05140295470499012 1102 4 0.179426249626502 1102 5 0.14567361123556566 1102 6 0.23318896974932102 1102 7 0.25505658936503295 1102 8 0.42360426776428284 1102 9 0.28136468242190205 1102 10-11 0.30820412214515613 1102 12-13 0.31787400925141895 1102 14-15 0.3783380919241921 1102 16-17 0.3937133774419266 1102 18-19 0.32706925274860765 1102 20-21 0.2901108036714888 1102 22-23 0.384317391299291 1102 24-25 0.39216954767752554 1102 26-27 0.5703470528793133 1102 28-29 0.21538807879393573 1102 30-31 0.24213947952603831 1102 32-33 0.3075082834122256 1102 34-35 0.23032868728642342 1102 36-37 0.25904526909481973 1102 38-39 0.3449979592338579 1102 40-41 0.4168868481904866 1102 42-43 0.2878223673819633 1102 44-45 0.14954107302619235 1102 46-47 0.19955522855146057 1102 48-49 0.18325057251065147 1102 50-51 0.24806679446707847 1102 52-53 0.327146191203326 1102 54-55 0.3407188068835225 1102 56-57 0.5121784355690835 1102 58-59 0.5158480539026868 1102 60-61 0.5212274743479455 1102 62-63 0.5067370485082563 1102 64-65 0.47609747322508866 1102 66-67 0.4120167961627388 1102 68-69 0.3279995404487366 1102 70-71 0.43259438884255275 1102 72-73 0.4721842047819038 1102 74-75 0.43286931435735454 1102 76-77 0.31644051428226305 1102 78-79 0.32455689218386397 1102 80-81 0.2920846016898544 1102 82-83 0.5711549130145492 1102 84-85 0.4852074177822061 1102 86-87 0.5186927378797712 1102 88-89 0.6076701633929851 1102 90-91 0.6296928503695085 1102 92-93 0.54385292473604 1102 94-95 0.6462358585420382 1102 96-97 0.5914031209801323 1102 98-99 0.46813126782452485 1102 100-101 0.4486572718257982 2305 1 0.2037573301686848 2305 2 0.1938901175878236 2305 3 0.17632421915033447 2305 4 0.22132399682736548 2305 5 0.19090586088707795 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-0.5927318771515786 1213 72-73 -0.5287604818898899 1213 74-75 -0.9670913023249881 1213 76-77 -0.724330395637665 1213 78-79 -0.8384659911765411 1213 80-81 -1.44017996757292 1213 82-83 -1.6005638752787448 1213 84-85 -1.4816087459064882 1213 86-87 -1.3982529177014307 1213 88-89 -1.4502373342559842 1213 90-91 -1.5317039750273196 1213 92-93 -1.414699296631266 1213 94-95 -1.5749454130090719 1213 96-97 -1.6677684044822385 1213 98-99 -1.5533890080296828 1213 100-101 -1.4518342502168906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1003.0 3 275.0 4 332.0 5 346.0 6 639.0 7 2960.0 8 14342.0 9 42495.0 10 65033.0 11 52854.0 12 32706.0 13 21275.0 14 14983.0 15 12013.0 16 10624.0 17 9550.0 18 9201.0 19 9187.0 20 9547.0 21 10107.0 22 10521.0 23 11304.0 24 12599.0 25 14684.0 26 17411.0 27 21248.0 28 26282.0 29 31248.0 30 37869.0 31 50535.0 32 69917.0 33 101094.0 34 155246.0 35 269023.0 36 518489.0 37 752342.0 38 148025.0 39 50.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.21131067120138 20.418511691356308 24.593093039190745 11.777084598251564 2 34.0357997182538 24.1800678220082 31.352928092281633 10.431204367456361 3 39.68168018681919 16.24107509985043 30.19450450077906 13.882740212551308 4 31.71903027900818 13.916312369942737 35.018456720142126 19.34620063090695 5 36.35858923004115 12.620483467161586 34.98014717271316 16.040780130084105 6 31.538953717467045 13.101667076315657 40.926860099018015 14.432519107199287 7 39.05836340348923 15.976652142508357 28.868207693903752 16.096776760098656 8 30.791613893446968 32.15987009988957 21.628298196247748 15.420217810415718 9 36.92861111923493 27.409547763187398 19.45435225533491 16.20748886224276 10-11 31.44489168317253 28.31816470847839 24.675102149182543 15.561841459166544 12-13 33.18875329702743 21.691997913304633 24.721450545807354 20.39779824386058 14-15 28.659413147778018 22.60689152536226 26.30711814664618 22.426577180213542 16-17 29.84472947609334 24.961392441711407 27.660909342765905 17.532968739429347 18-19 29.387299835077734 25.157810860147904 27.532952771479813 17.92193653329455 20-21 27.30587466719018 28.488539956965987 26.588777585662037 17.616807790181795 22-23 31.82401009087834 24.12320385387332 25.744879746792254 18.307906308456083 24-25 28.005829159609924 27.850240132969635 26.045207875586684 18.09872283183375 26-27 24.20291817114293 29.341804699494478 27.389135654578308 19.066141474784285 28-29 25.22725482042201 28.93721481340715 25.393417953556046 20.44211241261479 30-31 26.735385648088762 26.476781571223313 26.78745790728092 20.000374873407008 32-33 23.095760570840216 27.163520504219274 29.373053829532264 20.36766509540825 34-35 29.276198073830756 24.309741171984754 26.17439910739261 20.23966164679188 36-37 23.44926313486995 28.49094005476199 29.135545686419263 18.924251123948803 38-39 23.02077599754742 30.646457505719653 24.89278269094783 21.439983805785094 40-41 29.577938939321886 25.07781174422962 23.872799436980987 21.471449879467507 42-43 30.591674592852087 25.57812286818842 24.75198174182176 19.07822079713774 44-45 24.530143240029524 29.30758527015616 25.692236935115865 20.47003455469845 46-47 25.611226792025477 29.464759446125644 25.691878808961032 19.232134952887847 48-49 22.126272867848034 27.472312944193238 29.06645968182725 21.334954506131474 50-51 19.80305746994343 26.299311367613676 29.149237821502826 24.74839334094007 52-53 26.532365125036574 26.905478524970867 25.20272357929334 21.359432770699225 54-55 27.85217708332926 26.55784929715223 26.045538080152742 19.544435539365768 56-57 29.051016440085437 25.939203145365685 24.161962101626422 20.84781831292246 58-59 26.03575593300882 24.714330822883547 25.08040551736212 24.16950772674551 60-61 21.13162397837196 24.785907393227173 26.705103061368007 27.377365567032864 62-63 25.188993937073327 24.826742126839903 27.022211195364555 22.962052740722218 64-65 22.684976348713327 28.345207002726642 28.186437740940217 20.783378907619813 66-67 20.79504495351469 27.9434118717293 26.650861556929655 24.610681617826348 68-69 19.873861036543925 24.009577246537713 34.445755732936576 21.67080598398179 70-71 19.57339341300896 31.241737351702064 29.41604367991399 19.76882555537498 72-73 21.381103270155176 34.73083855000387 25.194448415702432 18.693609764138525 74-75 22.512521746909435 33.57757974941076 25.116466944404674 18.793431559275138 76-77 21.598763181284912 32.40565419581492 25.156745796529183 20.838836826370986 78-79 21.8025978419485 33.465521784335095 25.054597808352852 19.67728256536355 80-81 21.130199628543544 34.02500884885223 24.786982928161382 20.057808594442843 82-83 20.770069441459327 32.848154936127145 25.728744543745773 20.653031078667755 84-85 21.351893049641287 31.594978388202666 26.036735170782244 21.016393391373803 86-87 19.709492212142933 31.828629828323873 27.14067650212947 21.321201457403724 88-89 19.560878672533864 31.9912969056533 27.146385499792874 21.301438922019962 90-91 21.09624626777897 30.6501459674038 26.08475214066639 22.168855624150837 92-93 21.224001268974053 31.10351905883382 25.021722585393984 22.650757086798137 94-95 21.614599712706205 32.193605701183984 24.88132625503516 21.31046833107465 96-97 20.924775879908893 33.19313173624861 23.879926862889008 22.002165520953483 98-99 21.343161531951868 30.844272293169073 25.438678401222738 22.373887773656325 100-101 22.705917631970433 31.469068766354972 24.94972020109966 20.875293400574932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 503.0 1 443.5 2 311.5 3 238.0 4 232.5 5 226.5 6 266.5 7 370.5 8 490.0 9 623.0 10 748.0 11 895.0 12 995.0 13 960.0 14 886.0 15 831.0 16 750.5 17 650.5 18 596.5 19 572.0 20 559.0 21 580.0 22 629.5 23 716.5 24 863.5 25 987.5 26 1210.5 27 1521.5 28 1848.5 29 2290.0 30 2968.0 31 4154.5 32 6427.0 33 10295.0 34 16746.0 35 27618.0 36 43417.5 37 63787.5 38 88048.5 39 105813.0 40 113988.0 41 116936.0 42 145337.5 43 181732.0 44 175836.0 45 157242.0 46 157276.5 47 151685.5 48 130003.0 49 113109.0 50 100566.5 51 94557.5 52 93881.5 53 96038.0 54 83175.0 55 55367.0 56 39598.0 57 33239.0 58 28726.5 59 23518.5 60 18494.0 61 14655.0 62 11550.5 63 8911.0 64 7001.0 65 5493.5 66 4177.0 67 3274.0 68 2598.0 69 1942.0 70 1480.0 71 1162.0 72 792.0 73 504.5 74 374.5 75 291.5 76 216.0 77 156.0 78 119.5 79 90.0 80 78.0 81 69.5 82 59.5 83 49.0 84 39.5 85 25.0 86 18.0 87 21.0 88 19.0 89 20.5 90 15.5 91 6.0 92 6.5 93 6.0 94 4.0 95 3.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17960090505457163 2 0.18575508917919153 3 0.15677589304807002 4 0.15833391434544214 5 0.16546186178091962 6 0.16316378036729573 7 0.13067903631708694 8 0.1140082084352052 9 0.11233333554053018 10-11 0.09194273181117249 12-13 0.13619053665654082 14-15 0.11484564488254273 16-17 0.10791245010923678 18-19 0.109743125133649 20-21 0.10296573249008027 22-23 0.10386159473606923 24-25 0.4513392945824873 26-27 0.30823503841885763 28-29 0.38687616340371567 30-31 0.25296423289458153 32-33 0.28056068512428534 34-35 0.29863373217380196 36-37 0.28001537767020507 38-39 0.32798295836304936 40-41 0.3232115181398472 42-43 0.3654338952986318 44-45 0.320484980869446 46-47 0.3441863798557194 48-49 0.33955126649603734 50-51 0.3544498451521583 52-53 0.3461728570098689 54-55 0.32212090323168674 56-57 0.3378569183351452 58-59 0.3293267517320328 60-61 0.3006591598603857 62-63 0.3097151586513612 64-65 0.3110005262216932 66-67 0.32215985376412104 68-69 0.3100267629108356 70-71 0.2978547215251159 72-73 0.2842220351731098 74-75 0.29261587491270213 76-77 0.2820992311554403 78-79 0.29226532012079337 80-81 0.30011385240630545 82-83 0.32597700594268275 84-85 0.32484744050208797 86-87 0.3174663146057875 88-89 0.33104057515914215 90-91 0.3204070798045774 92-93 0.30424260884434157 94-95 0.29641355182504664 96-97 0.2818460526946173 98-99 0.3058785312065823 100-101 0.3089361480026751 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2567359.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.96232392089652 #Duplication Level Percentage of deduplicated Percentage of total 1 87.01580460827545 36.51385379209894 2 5.655315394904558 4.746203529516357 3 2.0492055677393606 2.5796828344195113 4 1.1131474825257857 1.8684102093391004 5 0.7534353689847406 1.580794950339894 6 0.5173242939448943 1.3024877756798845 7 0.3554162068438759 1.0439862998823355 8 0.25861516922009214 0.8681674801336778 9 0.26084428235393825 0.9851069042144774 >10 1.5918237787117664 13.617593450993414 >50 0.2245248162958237 6.596294321570098 >100 0.18295482104154903 15.37186353099433 >500 0.014183170092567211 4.032009193690426 >1k 0.006650155573755603 4.548299124189996 >5k 1.8872087312956897E-4 0.4337723400130848 >10k+ 5.661626185795651E-4 3.9114742629244867 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 29846 1.1625175910342107 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23317 0.9082095647706456 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11662 0.45424110924884287 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11391 0.44368551495914677 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 11355 0.4422832957915119 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 11214 0.43679127071827506 Illumina Single End PCR Primer 1 (97% over 34bp) TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5825 0.2268868514298156 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5130 0.19981623138797494 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4804 0.18711835781439215 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3331 0.12974422353866366 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3229 0.12577126923036475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005102519748893707 0.0 0.0 2 0.005530975605671042 0.0 0.0 3 0.006115233592185588 0.0 0.0 4 0.006465788384094316 0.0 0.0 5 0.0072837495652146814 0.0 0.0 6 0.007829057019294925 0.0 0.0 7 0.008841770862586806 0.0 0.0 8 0.009854484705878687 0.0 0.0 9 0.01102300067890778 0.0 0.0 10-11 0.016515025752144516 0.0 0.0 12-13 0.021383842306432407 0.0 0.0 14-15 0.02638898572424036 0.0 0.0 16-17 0.030342464766322123 0.0 0.0 18-19 0.03595134143686177 0.0 1.9475266217151557E-5 20-21 0.04080068272493251 0.0 3.895053243430311E-5 22-23 0.04627323253195209 0.0 3.895053243430311E-5 24-25 0.05143417807949725 0.0 3.895053243430311E-5 26-27 0.05714043108112266 0.0 3.895053243430311E-5 28-29 0.06403467532199432 0.0 3.895053243430311E-5 30-31 0.11914967871653322 0.0 3.895053243430311E-5 32-33 0.12785512271559996 0.0 3.895053243430311E-5 34-35 0.13572313026732918 0.0 3.895053243430311E-5 36-37 0.14423382160422443 0.0 3.895053243430311E-5 38-39 0.15369880098576005 0.0 3.895053243430311E-5 40-41 0.16481917799575363 0.0 3.895053243430311E-5 42-43 0.17621220873278726 0.0 3.895053243430311E-5 44-45 0.1895527660915361 0.0 3.895053243430311E-5 46-47 0.2030491255800221 0.0 3.895053243430311E-5 48-49 0.2188630417483492 0.0 3.895053243430311E-5 50-51 0.23639078134378558 0.0 3.895053243430311E-5 52-53 0.2548728089838624 0.0 3.895053243430311E-5 54-55 0.2747765310577913 0.0 3.895053243430311E-5 56-57 0.29828317738189325 0.0 3.895053243430311E-5 58-59 0.3235815481979731 0.0 3.895053243430311E-5 60-61 0.34872411688431576 0.0 3.895053243430311E-5 62-63 0.3769437776329684 0.0 3.895053243430311E-5 64-65 0.4088053131642283 0.0 3.895053243430311E-5 66-67 0.44502930832813015 0.0 3.895053243430311E-5 68-69 0.4855768125922397 0.0 3.895053243430311E-5 70-71 0.5285197746010588 0.0 3.895053243430311E-5 72-73 0.5737997685559363 0.0 3.895053243430311E-5 74-75 0.6245912628502676 0.0 3.895053243430311E-5 76-77 0.6844971817342257 0.0 3.895053243430311E-5 78-79 0.7513168201252727 0.0 3.895053243430311E-5 80-81 0.8274845863005524 0.0 3.895053243430311E-5 82-83 0.9126499254681562 0.0 3.895053243430311E-5 84-85 1.0078060762051586 0.0 3.895053243430311E-5 86-87 1.1162443585022586 0.0 3.895053243430311E-5 88-89 1.2311094786510184 0.0 3.895053243430311E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3060 0.0 89.04628 9 ATCGGGA 4890 0.0 88.88735 2 CGGGAGA 4085 0.0 87.13857 4 AGAGCGG 3400 0.0 81.81418 8 TCGGGAG 4655 0.0 81.55966 3 ATTCGGA 595 0.0 81.25736 2 GTTCGGA 555 0.0 79.42724 2 AAGCGGC 815 0.0 77.33275 9 CGGAGAG 1500 0.0 76.78521 5 ATCCGGA 680 0.0 74.585495 2 TCGGGAA 1200 0.0 72.67736 3 TTGGGAG 2390 0.0 71.39489 3 TCGGAGA 920 0.0 70.58222 4 CCGGAGA 775 0.0 70.332924 4 GAGAGCG 4635 0.0 70.24006 7 AATCGGA 670 0.0 69.33153 2 GTCCGGA 370 0.0 69.178566 2 AAGCGTC 355 0.0 66.74382 9 GGTCCGG 465 0.0 65.24016 1 GATCCGA 125 0.0 64.46543 1 >>END_MODULE