FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567359
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA298461.1625175910342107No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC233170.9082095647706456No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC116620.45424110924884287No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT113910.44368551495914677No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT113550.4422832957915119No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT112140.43679127071827506Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT58250.2268868514298156No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51300.19981623138797494No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT48040.18711835781439215No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33310.12974422353866366No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG32290.12577126923036475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC30600.089.046289
ATCGGGA48900.088.887352
CGGGAGA40850.087.138574
AGAGCGG34000.081.814188
TCGGGAG46550.081.559663
ATTCGGA5950.081.257362
GTTCGGA5550.079.427242
AAGCGGC8150.077.332759
CGGAGAG15000.076.785215
ATCCGGA6800.074.5854952
TCGGGAA12000.072.677363
TTGGGAG23900.071.394893
TCGGAGA9200.070.582224
CCGGAGA7750.070.3329244
GAGAGCG46350.070.240067
AATCGGA6700.069.331532
GTCCGGA3700.069.1785662
AAGCGTC3550.066.743829
GGTCCGG4650.065.240161
GATCCGA1250.064.465431
Produced by FastQC (version 0.11.2)