FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139482
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG26270.12278673061984163No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25680.12002905376161145No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC25140.11750507833204486No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23410.1094190089002852No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA21530.10063183518253484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA7500.080.330462
CGATCGG200.002083522571.170541
GAGCGGC6450.069.845599
CGGGAGA8000.068.182744
AGAGCGG6900.063.2286388
GATCGGG10350.060.970571
CGAGCGC250.005044094356.9364281
GAGAGCG8500.056.3478747
TCGGGAA2050.050.9020653
TCGGGAG11400.049.5117953
TTGTGTT274150.048.234491
GTTCGGA1300.047.4392472
GAGCGTC19100.044.6903959
AGAGCGT19400.044.2437488
AAGCGGC2450.042.5825549
GGTCCGG1155.638867E-1141.258281
AGCGGCG6250.040.97215710-11
ATTCGGA1305.456968E-1240.14092
GGCGCCG5500.039.72855856-57
GAACCGG1208.731149E-1139.5391851
Produced by FastQC (version 0.11.2)