##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_30230-1.34200000035711.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2185036 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9033128058302 33.0 33.0 33.0 27.0 33.0 2 31.737135223401353 33.0 33.0 33.0 33.0 33.0 3 31.680954455670296 33.0 33.0 33.0 33.0 33.0 4 35.35831492021184 37.0 37.0 37.0 33.0 37.0 5 35.397188879267894 37.0 37.0 37.0 33.0 37.0 6 35.54437272429379 37.0 37.0 37.0 33.0 37.0 7 35.31621675798477 37.0 37.0 37.0 33.0 37.0 8 35.360914877375016 37.0 37.0 37.0 33.0 37.0 9 35.15885047202884 37.0 37.0 37.0 33.0 37.0 10-11 35.0983006687304 37.0 37.0 37.0 33.0 37.0 12-13 35.04950833762007 37.0 37.0 37.0 33.0 37.0 14-15 36.7126573658283 40.0 37.0 40.0 33.0 40.0 16-17 36.619124124270726 40.0 37.0 40.0 33.0 40.0 18-19 36.714533765118745 40.0 37.0 40.0 33.0 40.0 20-21 36.706567992472436 40.0 37.0 40.0 33.0 40.0 22-23 36.86770172207689 40.0 37.0 40.0 33.0 40.0 24-25 36.510930712354394 40.0 37.0 40.0 33.0 40.0 26-27 36.375828590467165 40.0 37.0 40.0 33.0 40.0 28-29 36.26379496722251 40.0 37.0 40.0 33.0 40.0 30-31 36.30519680224948 40.0 37.0 40.0 33.0 40.0 32-33 36.305394053004164 40.0 37.0 40.0 33.0 40.0 34-35 36.237953745384516 40.0 37.0 40.0 33.0 40.0 36-37 36.048143829209224 40.0 37.0 40.0 33.0 40.0 38-39 36.01462515949394 38.5 37.0 40.0 30.0 40.0 40-41 35.77273028911194 37.0 37.0 40.0 27.0 40.0 42-43 35.68707403447815 37.0 37.0 40.0 27.0 40.0 44-45 35.525954034624604 37.0 37.0 40.0 27.0 40.0 46-47 35.430077811074966 37.0 37.0 40.0 27.0 40.0 48-49 35.30412862762902 37.0 37.0 40.0 27.0 40.0 50-51 34.096920828764375 37.0 35.0 38.5 24.5 38.5 52-53 34.56009786566446 37.0 35.0 38.5 27.0 40.0 54-55 35.30349934737917 37.0 37.0 40.0 27.0 40.0 56-57 35.319181926522035 37.0 37.0 40.0 27.0 40.0 58-59 35.04669145039258 37.0 37.0 40.0 27.0 40.0 60-61 34.86950420954163 37.0 35.0 40.0 27.0 40.0 62-63 34.59591855694826 37.0 33.0 40.0 27.0 40.0 64-65 34.37792352162619 37.0 33.0 37.0 27.0 40.0 66-67 34.09304583540042 37.0 33.0 37.0 27.0 40.0 68-69 33.92922725300636 37.0 33.0 37.0 27.0 40.0 70-71 33.59695469548328 37.0 33.0 37.0 27.0 37.0 72-73 33.44227211817105 37.0 33.0 37.0 27.0 37.0 74-75 33.302243075171305 37.0 33.0 37.0 27.0 37.0 76-77 33.01861845754486 37.0 33.0 37.0 27.0 37.0 78-79 32.848456959061544 37.0 33.0 37.0 22.0 37.0 80-81 32.65913216075158 37.0 33.0 37.0 22.0 37.0 82-83 32.30706427720184 37.0 33.0 37.0 22.0 37.0 84-85 32.16322522832576 33.0 33.0 37.0 22.0 37.0 86-87 32.037562539015376 33.0 33.0 37.0 22.0 37.0 88-89 31.965347939347453 33.0 33.0 37.0 22.0 37.0 90-91 31.87203711975455 33.0 33.0 37.0 22.0 37.0 92-93 31.72394070395179 33.0 33.0 37.0 15.0 37.0 94-95 31.500364296057363 33.0 33.0 37.0 15.0 37.0 96-97 31.332349672957335 33.0 33.0 37.0 15.0 37.0 98-99 31.2064492301271 33.0 33.0 37.0 15.0 37.0 100-101 29.850524201889577 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.19966004360278689 2306 2 0.11056375865995705 2306 3 0.07292428753735081 2306 4 0.18504773355877546 2306 5 0.1525848534163856 2306 6 0.2645381596548475 2306 7 0.4020843796759621 2306 8 0.18327799261508204 2306 9 0.2688525036556584 2306 10-11 0.24556474727489785 2306 12-13 0.3127121876347019 2306 14-15 0.5147279280681758 2306 16-17 0.5762972971525429 2306 18-19 0.5844988707794556 2306 20-21 0.5297611176386425 2306 22-23 0.44721927574246934 2306 24-25 0.38215170827616873 2306 26-27 0.49003297409200997 2306 28-29 0.263615574389803 2306 30-31 0.2708733339006102 2306 32-33 0.22463556632929738 2306 34-35 0.24608449324712467 2306 36-37 0.1866390862699916 2306 38-39 0.2035833274809704 2306 40-41 0.26448855833273655 2306 42-43 0.21837348728649175 2306 44-45 0.2720922715850804 2306 46-47 0.2707983277067214 2306 48-49 0.3868735514377306 2306 50-51 0.2828329272827119 2306 52-53 0.18878093711162336 2306 54-55 0.3211929278173429 2306 56-57 0.2627629847007853 2306 58-59 0.44041507154592097 2306 60-61 0.36613877153524754 2306 62-63 0.5000743066498501 2306 64-65 0.4058860306688459 2306 66-67 0.3967155786360763 2306 68-69 0.27034086295034143 2306 70-71 0.3480330058045169 2306 72-73 0.4218857085371184 2306 74-75 0.23860949319531244 2306 76-77 0.260427809070805 2306 78-79 0.15908720165940338 2306 80-81 0.4235612179028152 2306 82-83 0.30735977521698743 2306 84-85 0.26011649455841734 2306 86-87 0.21546875860625647 2306 88-89 0.3804360406187257 2306 90-91 0.28632987988740766 2306 92-93 0.20855489170931207 2306 94-95 0.32429867952648195 2306 96-97 0.28783303398013516 2306 98-99 0.2040834090855732 2306 100-101 0.06203897904730482 2307 1 0.18981191254858132 2307 2 0.1506019039928468 2307 3 0.08500937874854131 2307 4 0.28435155714314675 2307 5 0.1743470342667024 2307 6 0.21865776268734294 2307 7 0.285631675380408 2307 8 0.1903832663691034 2307 9 0.2821613841465833 2307 10-11 0.28141100134745045 2307 12-13 0.21633327549251646 2307 14-15 0.4482576894464714 2307 16-17 0.42342309587637317 2307 18-19 0.5660637379780127 2307 20-21 0.448423353518173 2307 22-23 0.25199029878005774 2307 24-25 0.3712351706362256 2307 26-27 0.28842263809715263 2307 28-29 0.25111750591886306 2307 30-31 0.37290590472878904 2307 32-33 0.3364293709132795 2307 34-35 0.30555442316832426 2307 36-37 0.1611809310187695 2307 38-39 0.29988308564836785 2307 40-41 0.5664874715142503 2307 42-43 0.4536024091852724 2307 44-45 0.41752290190194685 2307 46-47 0.37604042013860095 2307 48-49 0.3563068674833687 2307 50-51 0.27772560309659866 2307 52-53 0.314030573261455 2307 54-55 0.40034019172254176 2307 56-57 0.4088000032940542 2307 58-59 0.5215133088557309 2307 60-61 0.34666431263009656 2307 62-63 0.23348612860571905 2307 64-65 0.3937826996286802 2307 66-67 0.45923087170022825 2307 68-69 0.3782870960642981 2307 70-71 0.40493180813604823 2307 72-73 0.2936825966846328 2307 74-75 0.2711632860645068 2307 76-77 0.311326395965871 2307 78-79 0.2717358691710672 2307 80-81 0.4368640758086926 2307 82-83 0.18277468830763866 2307 84-85 0.35028336834465534 2307 86-87 0.3078299182326347 2307 88-89 0.3583901099761846 2307 90-91 0.5429026710871376 2307 92-93 0.3772352813808908 2307 94-95 0.5574993668582984 2307 96-97 0.4667865771357427 2307 98-99 0.25639282954425724 2307 100-101 0.06138251727815813 2201 1 0.09257142945661556 2201 2 0.09864191104064446 2201 3 0.04602705993506362 2201 4 0.21592974790812036 2201 5 0.13640153000272193 2201 6 0.07465217279393244 2201 7 0.2840522824194309 2201 8 0.16544183366949738 2201 9 0.2519827658069218 2201 10-11 0.151406701429444 2201 12-13 0.19571784018624072 2201 14-15 0.4184456136737751 2201 16-17 0.38917339022356856 2201 18-19 0.40116251841695316 2201 20-21 0.3721926769108137 2201 22-23 0.2957109554971211 2201 24-25 0.26567822630043736 2201 26-27 0.2914780606054421 2201 28-29 0.25644040368194965 2201 30-31 0.19622634466892208 2201 32-33 0.16336005636218687 2201 34-35 0.07264325782058023 2201 36-37 0.11481289874647871 2201 38-39 0.184204477804073 2201 40-41 0.2825216176974692 2201 42-43 0.23425109252620047 2201 44-45 0.22214823974129416 2201 46-47 0.22078777769268498 2201 48-49 0.15193004709463764 2201 50-51 0.1677559951206291 2201 52-53 0.2193477780857549 2201 54-55 0.43100795753984755 2201 56-57 0.41306684501063273 2201 58-59 0.48864787784301456 2201 60-61 0.46424162687331716 2201 62-63 0.5160333593249504 2201 64-65 0.458320429819004 2201 66-67 0.4866046840546048 2201 68-69 0.3495099265934627 2201 70-71 0.45244447094933093 2201 72-73 0.25853267520902534 2201 74-75 0.2870139860415435 2201 76-77 0.3669914107261647 2201 78-79 0.19849382640470026 2201 80-81 0.262835071716772 2201 82-83 0.24362806937261183 2201 84-85 0.34744755333527166 2201 86-87 0.38252922611121676 2201 88-89 0.40233196439852037 2201 90-91 0.4086841652832014 2201 92-93 0.2419592883447237 2201 94-95 0.35543200317237833 2201 96-97 0.08787427192806874 2201 98-99 0.007483234048233811 2201 100-101 -0.06376910889867204 1101 1 0.23295959045703185 1101 2 0.14510692637528777 1101 3 0.20181815248031754 1101 4 0.25801496281583525 1101 5 0.3386888118959632 1101 6 0.30421612257111263 1101 7 0.39134670464620314 1101 8 0.2683582368415287 1101 9 0.42039755238369025 1101 10-11 0.3183531919777778 1101 12-13 0.34655226461003963 1101 14-15 0.48481998685343797 1101 16-17 0.47648957488061683 1101 18-19 0.5644457655021853 1101 20-21 0.4411896752986664 1101 22-23 0.42439268069671243 1101 24-25 0.39931647117380464 1101 26-27 0.3998579322872686 1101 28-29 0.3981047694095423 1101 30-31 0.5001046888240879 1101 32-33 0.410441871817973 1101 34-35 0.24027038099355735 1101 36-37 0.17995723442825806 1101 38-39 0.27451678203869534 1101 40-41 0.23381311253594106 1101 42-43 0.13900631714239609 1101 44-45 0.04312419418566549 1101 46-47 0.08748279786672697 1101 48-49 0.061401009243446936 1101 50-51 0.19235926868892506 1101 52-53 0.0679415331886446 1101 54-55 0.32087937215719364 1101 56-57 0.16944371211136655 1101 58-59 0.010504720835847081 1101 60-61 0.17496073713728322 1101 62-63 0.3377159247384185 1101 64-65 0.32115195060612933 1101 66-67 0.2551492139059448 1101 68-69 0.3440163232041016 1101 70-71 0.28048698042771747 1101 72-73 0.14743294508691207 1101 74-75 0.2531650939066026 1101 76-77 0.14847092939940154 1101 78-79 -0.03577591491037424 1101 80-81 0.1421001963089097 1101 82-83 0.19595884643684514 1101 84-85 -0.010257475181532527 1101 86-87 0.23741508468808092 1101 88-89 0.23506170945446314 1101 90-91 0.05099977099428088 1101 92-93 0.0688603835917263 1101 94-95 0.1933519245544879 1101 96-97 0.0960203385098275 1101 98-99 0.06528282373883698 1101 100-101 -0.16484520614971743 2304 1 -0.04570228929508957 2304 2 -0.12245284596242456 2304 3 -0.18887016334616646 2304 4 -0.26044057052043 2304 5 -0.2913665654169222 2304 6 -0.28584755035114284 2304 7 -0.028845412876762566 2304 8 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70-71 0.06765611746458688 2304 72-73 -0.02752200590856546 2304 74-75 -0.02619119976895945 2304 76-77 0.08652772410778908 2304 78-79 -6.00608357387955E-4 2304 80-81 -0.03863668454197011 2304 82-83 6.234785732672776E-4 2304 84-85 0.026381786674051 2304 86-87 0.012963638724905024 2304 88-89 0.038980011436628104 2304 90-91 0.001189169095628273 2304 92-93 -0.09104641441293282 2304 94-95 -0.11091962754852958 2304 96-97 0.010748616099593988 2304 98-99 -0.11987201847977147 2304 100-101 0.12895794618184553 2202 1 0.15894739844886274 2202 2 0.13376788003289164 2202 3 0.15582311013809047 2202 4 0.15124951534998132 2202 5 0.17483822250918024 2202 6 0.2637556242520347 2202 7 0.3347051986246683 2202 8 0.3278057133298873 2202 9 0.19384323092319988 2202 10-11 0.3182002599381164 2202 12-13 0.22303996089499378 2202 14-15 0.30625015409459166 2202 16-17 0.25000187859566125 2202 18-19 0.20941164170967852 2202 20-21 0.21748268244791547 2202 22-23 0.1736467712963119 2202 24-25 0.2746817792720151 2202 26-27 0.2506477265338276 2202 28-29 0.30341922427995627 2202 30-31 0.30355377176378795 2202 32-33 0.12479647201372046 2202 34-35 0.10771154870281663 2202 36-37 0.08989595522488969 2202 38-39 0.1678296623740252 2202 40-41 0.28053172290234585 2202 42-43 0.2525696602633829 2202 44-45 0.2286658661717027 2202 46-47 0.21987646355463397 2202 48-49 0.30524423129544687 2202 50-51 0.23150761010770537 2202 52-53 0.22705934139325024 2202 54-55 0.34240861276317247 2202 56-57 0.44878113072491743 2202 58-59 0.3679333614162914 2202 60-61 0.4470708535252186 2202 62-63 0.34995500938031654 2202 64-65 0.30741719061634853 2202 66-67 0.27121731786781567 2202 68-69 0.344826464061164 2202 70-71 0.4165859899600335 2202 72-73 0.2551469701518059 2202 74-75 0.16565924259377596 2202 76-77 0.39172955210674587 2202 78-79 0.34901639008133145 2202 80-81 0.35857496651190246 2202 82-83 0.364515620129346 2202 84-85 0.26307024621087294 2202 86-87 0.3969775926671346 2202 88-89 0.41704565026045515 2202 90-91 0.48841838455230757 2202 92-93 0.40663606021626464 2202 94-95 0.42676252181394503 2202 96-97 0.43695372929241216 2202 98-99 0.4109185422800543 2202 100-101 0.5258280671810631 1102 1 0.1016578556098402 1102 2 -0.08668967739540889 1102 3 0.06268524446216617 1102 4 0.061240283779142146 1102 5 0.0064177461676777625 1102 6 0.16030492348330228 1102 7 0.06991250308149688 1102 8 0.09176681235877737 1102 9 0.017933666336183762 1102 10-11 0.12024300456531023 1102 12-13 0.18132586207754287 1102 14-15 0.21470751896251983 1102 16-17 0.20774832755584072 1102 18-19 0.23890287643700248 1102 20-21 0.1981905400438464 1102 22-23 0.2872562116630064 1102 24-25 0.12240593276150946 1102 26-27 0.22864463612862096 1102 28-29 0.053989118031935845 1102 30-31 0.03524165782845756 1102 32-33 0.15861111188807087 1102 34-35 0.09727175591288528 1102 36-37 0.06784212893700214 1102 38-39 0.01766820427667426 1102 40-41 -0.010198335771939071 1102 42-43 -0.07265537107488029 1102 44-45 0.025814367976487063 1102 46-47 -0.03266882589004183 1102 48-49 -0.040353734738410196 1102 50-51 0.19421579241329567 1102 52-53 0.07415672974599374 1102 54-55 0.19873615607038175 1102 56-57 0.40066369155134396 1102 58-59 0.31654263187677856 1102 60-61 0.3814986188432101 1102 62-63 0.3312186018772536 1102 64-65 0.27516969956474924 1102 66-67 0.32250610368026855 1102 68-69 0.27503937542298473 1102 70-71 0.2618639659617301 1102 72-73 0.33001024284750713 1102 74-75 0.21124319136517045 1102 76-77 0.23800572710259615 1102 78-79 0.21768242809501004 1102 80-81 0.25863825657577877 1102 82-83 0.38962044282277475 1102 84-85 0.3559383857756018 1102 86-87 0.4381154262496736 1102 88-89 0.32634806255109794 1102 90-91 0.4661266895679326 1102 92-93 0.2435395478244402 1102 94-95 0.32745068916778663 1102 96-97 0.3414338729197972 1102 98-99 0.27780032908929186 1102 100-101 0.3009557800169098 2305 1 0.1859154976662616 2305 2 0.12652636922243943 2305 3 0.12203491983875736 2305 4 0.14793757079500125 2305 5 0.14611535917629226 2305 6 0.24302315531274132 2305 7 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-1.603388811146818 1213 92-93 -1.6565299689004327 1213 94-95 -1.7117103481692588 1213 96-97 -1.7434753054607626 1213 98-99 -1.6494341141143387 1213 100-101 -1.5601411427930536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 840.0 3 172.0 4 177.0 5 231.0 6 463.0 7 1961.0 8 9137.0 9 16059.0 10 18104.0 11 15809.0 12 10928.0 13 7970.0 14 6390.0 15 5988.0 16 5583.0 17 5592.0 18 5883.0 19 6299.0 20 7088.0 21 7579.0 22 8309.0 23 9110.0 24 10292.0 25 12117.0 26 14494.0 27 17510.0 28 21277.0 29 25960.0 30 32561.0 31 43380.0 32 60614.0 33 88299.0 34 136932.0 35 245278.0 36 476897.0 37 693107.0 38 156513.0 39 133.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.06138178710496 20.823684163471416 27.036228917203818 12.078705132219799 2 36.424530646343065 19.448064125360272 33.14214998959182 10.985255238704838 3 43.33314234244971 15.133857870696115 27.43351798331962 14.099481803534552 4 35.95327981870054 13.591958870252075 34.7958180844744 15.658943226572985 5 35.1644774993204 12.68085098580147 36.722130462546694 15.432541052331436 6 27.327415016255046 13.386578628414888 43.93616982263385 15.349836532696212 7 37.43577523394037 14.22875289847767 31.35873301010916 16.976738857472803 8 33.765807720027475 26.76846469530922 23.13900622068519 16.326721363978123 9 35.04912797557003 27.534804232634535 20.33103252365909 17.085035268136345 10-11 31.142631961207506 26.588439438488194 26.116290910740013 16.15263768956429 12-13 31.563217426831198 22.73422299702506 26.212701251867216 19.489858324276526 14-15 29.076966040775442 23.435743496778603 26.430339867157816 21.05695059528814 16-17 27.752575562548493 26.120269201350542 27.751109473978875 18.37604576212209 18-19 26.981250376552513 26.383004361022174 27.180047195345246 19.455698067080064 20-21 25.732180338041626 28.00756008912494 27.26538675362617 18.994872819207263 22-23 27.517102753964302 25.268507224217018 27.2712445811214 19.943145440697283 24-25 27.33134967388703 25.489939591339795 27.672164221806018 19.50654651296716 26-27 23.91810069486605 26.02122218659356 29.43626400640673 20.62441311213366 28-29 23.378680061232213 27.19239095797902 27.213435797501774 22.215493183287 30-31 22.863346894723172 27.323256055131502 27.920027569421435 21.89336948072389 32-33 22.22403019150602 24.93707042879319 30.330056650384236 22.50884272931655 34-35 22.335334275844513 26.926162535662662 28.235417531763847 22.50308565672898 36-37 21.20053565219786 29.548172551611607 28.423738985450868 20.827552810739665 38-39 20.444420564691857 27.868130495500274 28.052532700336197 23.63491623947167 40-41 22.75185594134277 27.654829006343252 26.14263833085706 23.45067672145692 42-43 25.129977015993248 27.00443922560941 26.735434925123958 21.13014883327338 44-45 21.676104226663075 29.303227735061366 26.34855167147327 22.67211636680229 46-47 22.356726090318407 29.38146567215662 27.06109788195448 21.200710355570486 48-49 22.056534218581252 26.990768690937962 28.742946886273185 22.209750204207605 50-51 20.658080531079957 27.173750274460517 27.220098502616906 24.94807069184262 52-53 22.800835392955747 28.31165926182459 26.404351686287626 22.48315365893204 54-55 23.248210435393947 27.948135057511507 27.560362576109735 21.24329193098481 56-57 24.760082990124545 27.476390641451204 25.111952851321607 22.651573517102648 58-59 22.79837063468555 26.158592169586253 25.977378090363644 25.065659105364553 60-61 20.467259835409102 26.043613764373468 28.501665489465218 24.987460910752215 62-63 22.347081787096204 26.051622989413463 28.525793703175275 23.075501520315058 64-65 22.725512320603134 28.360126402833846 27.331318448592317 21.583042827970704 66-67 22.04219468184543 27.831375564445192 26.81231478379743 23.314114969911945 68-69 21.244706970786037 24.673774927025356 30.894003969269722 23.18751413291888 70-71 21.054278242358482 29.206470244327036 28.168503272239143 21.57074824107534 72-73 23.537950862507607 29.929671450483013 26.405234098387442 20.127143588621937 74-75 24.761403950113113 28.103263544084378 26.90557793724476 20.229754568557745 76-77 23.348018632183294 27.330101588362414 26.61184083694269 22.7100389425116 78-79 23.671736870578854 28.780807577005028 26.364448267876067 21.18300728454005 80-81 22.7241654925326 29.68842177558453 26.226849588261135 21.360563143621732 82-83 22.47625690916309 29.328121559481886 26.904527322894324 21.2910942084607 84-85 22.92992387497173 28.518977300253596 26.778973374034376 21.772125450740297 86-87 20.5661809019268 29.182778788591897 27.947126449976178 22.30391385950513 88-89 20.012601430841038 29.437130731349114 28.441989675919583 22.10827816189027 90-91 21.556735410771378 28.538603268262726 27.09489671164785 22.80976460931805 92-93 22.0063527364124 28.679067441984852 26.019315312105668 23.29526450949708 94-95 22.667404863098533 29.970568548975752 25.73337611934543 21.628650468580286 96-97 21.326787540900682 31.26107792594374 24.660923377186407 22.751211155969166 98-99 21.5539734289037 29.157685136595013 26.49189814336565 22.79644329113564 100-101 23.207400333331805 29.774840335902958 26.224925505392537 20.792833825372703 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 488.0 1 408.5 2 278.5 3 207.0 4 183.5 5 194.5 6 224.5 7 308.5 8 431.0 9 533.5 10 642.0 11 798.0 12 911.5 13 895.0 14 841.5 15 765.0 16 677.0 17 607.0 18 568.5 19 564.5 20 571.0 21 619.0 22 684.0 23 797.5 24 947.5 25 1115.5 26 1373.5 27 1773.5 28 2235.5 29 2745.0 30 3521.0 31 4659.0 32 6342.5 33 9086.5 34 13593.0 35 22659.5 36 34608.0 37 47362.5 38 63415.0 39 77778.5 40 87434.0 41 94778.0 42 129396.5 43 165850.5 44 156167.0 45 134881.0 46 132352.0 47 128444.5 48 113332.5 49 102783.0 50 92925.5 51 87484.5 52 86515.5 53 87370.0 54 74864.0 55 47237.0 56 31048.0 57 25249.5 58 21806.0 59 18046.5 60 13798.0 61 10731.5 62 8295.5 63 6378.0 64 5076.5 65 3890.0 66 3003.5 67 2369.5 68 1888.5 69 1352.5 70 965.5 71 852.0 72 623.5 73 381.0 74 296.0 75 210.0 76 155.0 77 132.0 78 100.0 79 65.0 80 54.5 81 50.0 82 48.5 83 40.0 84 24.5 85 21.0 86 20.5 87 16.0 88 12.5 89 10.0 90 8.0 91 8.0 92 8.0 93 5.0 94 3.5 95 2.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17999703437380435 2 0.1857177639178485 3 0.15702258452492318 4 0.15798366708832257 5 0.1653519667410514 6 0.1629263774143767 7 0.13070722862232018 8 0.1134535083174831 9 0.11308738162666428 10-11 0.0932021257315669 12-13 0.13617624606642637 14-15 0.1153985563624581 16-17 0.1078929592006722 18-19 0.11008971934558516 20-21 0.10331637556543691 22-23 0.10404862894707455 24-25 0.4622120642405892 26-27 0.306745518151646 28-29 0.3995586342742179 30-31 0.265899509207171 32-33 0.29335901101858275 34-35 0.3117797601504048 36-37 0.2921462163552454 38-39 0.3413673733522011 40-41 0.33717979932596076 42-43 0.3800852709062917 44-45 0.33415925412670544 46-47 0.35772865984816726 48-49 0.3531978420492843 50-51 0.36786579260021346 52-53 0.35958217622043753 54-55 0.3359898875807996 56-57 0.350955316068019 58-59 0.3432895384789999 60-61 0.3133815644227372 62-63 0.32189400998427486 64-65 0.32395347262013074 66-67 0.3361271850898566 68-69 0.3228093267113219 70-71 0.310635614241596 72-73 0.29635667329966187 74-75 0.30516659679748986 76-77 0.29473198610915335 78-79 0.3045945238430854 80-81 0.3128094914683328 82-83 0.3387816035982931 84-85 0.3372255651623131 86-87 0.3294453729824131 88-89 0.34349548474258546 90-91 0.3328778107088396 92-93 0.3166767046401066 94-95 0.30910245872379216 96-97 0.29452603984556774 98-99 0.3183013918306151 100-101 0.3215507662116322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2185036.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.46562211045585 #Duplication Level Percentage of deduplicated Percentage of total 1 87.97395807201715 40.87764691335436 2 5.5657777906631605 5.172346551434445 3 1.7268134854555584 2.407123886112514 4 1.0190175069148282 1.8939712960097295 5 0.6344384012181533 1.4739787501682244 6 0.44981173098809546 1.2540469147765718 7 0.28816371959766535 0.9372794550537935 8 0.28141335289626807 1.0460837210011475 9 0.23278807637053983 0.9734978509608097 >10 1.4698291860689134 13.649464610923886 >50 0.2014868743797178 6.488869644845133 >100 0.14239705144964773 12.839698658096617 >500 0.008803131818311834 2.747212554025965 >1k 0.004503875231504957 3.4413829158640032 >5k 2.991543489457941E-4 0.7669310126343362 >10k+ 4.98590581552282E-4 4.030465264738484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31417 1.4378252806818743 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 21631 0.9899608061377478 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12040 0.5510206696823301 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11429 0.5230577436710425 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10469 0.47912254077278366 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5737 0.26255860315344914 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5653 0.25871427289985155 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5162 0.23624324725084622 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 4363 0.19967634400531617 Illumina Single End PCR Primer 1 (96% over 32bp) GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 3027 0.13853318663857253 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2926 0.13391083716698488 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2899 0.13267515958547135 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2699 0.12352199231500077 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2531 0.11583333180780545 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2399 0.10979224140929486 No Hit GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT 2322 0.10626827201016369 No Hit AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA 2240 0.10251547342927073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002425589326674709 0.0 0.0 2 0.002837481853845886 0.0 0.0 3 0.0032951402173694165 0.0 0.0 4 0.0035239693991311813 0.0 0.0 5 0.0037985644172452994 0.0 0.0 6 0.004210456944416476 0.0 0.0 7 0.004851178653349418 0.0 0.0 8 0.0054919003622823604 0.0 0.0 9 0.006178387907567656 0.0 0.0 10-11 0.009839654815755897 0.0 0.0 12-13 0.014004345923820018 0.0 0.0 14-15 0.01755119824112738 0.0 0.0 16-17 0.02073192386761591 0.0 0.0 18-19 0.024164361594042387 0.0 0.0 20-21 0.027230672629650038 0.0 0.0 22-23 0.03114365163777622 0.0 0.0 24-25 0.03530834274584034 0.0 0.0 26-27 0.03940438509937594 0.0 0.0 28-29 0.04388943706190653 0.0 0.0 30-31 0.09246987234992925 0.0 0.0 32-33 0.10109673250234778 0.0 0.0 34-35 0.10725223749173926 0.0 0.0 36-37 0.11459765422629192 0.0 0.0 38-39 0.12297280227877253 0.0 0.0 40-41 0.1330412862762902 0.0 0.0 42-43 0.1461531983912393 2.2882918176176504E-5 0.0 44-45 0.1597914176242405 4.576583635235301E-5 0.0 46-47 0.17528315322951202 4.576583635235301E-5 0.0 48-49 0.1905918254893741 4.576583635235301E-5 0.0 50-51 0.2099736571845956 4.576583635235301E-5 0.0 52-53 0.2306140493795068 4.576583635235301E-5 0.0 54-55 0.25372579673744505 4.576583635235301E-5 0.0 56-57 0.2817344885850851 4.576583635235301E-5 0.0 58-59 0.31381633986808455 4.576583635235301E-5 0.0 60-61 0.34926198012298193 4.576583635235301E-5 0.0 62-63 0.39040546700374734 4.576583635235301E-5 0.0 64-65 0.4358509425016338 4.576583635235301E-5 0.0 66-67 0.48241768099015303 4.576583635235301E-5 0.0 68-69 0.5358492949315252 4.576583635235301E-5 0.0 70-71 0.5958254234712839 4.576583635235301E-5 0.0 72-73 0.6580669609104839 4.576583635235301E-5 0.0 74-75 0.729393016865626 4.576583635235301E-5 0.0 76-77 0.812229180663385 4.576583635235301E-5 0.0 78-79 0.9044702238315525 4.576583635235301E-5 0.0 80-81 1.0045143420977962 4.576583635235301E-5 0.0 82-83 1.1206222689237157 4.576583635235301E-5 0.0 84-85 1.246272372629101 4.576583635235301E-5 0.0 86-87 1.3901372792027225 4.576583635235301E-5 0.0 88-89 1.5492193263635015 4.576583635235301E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCG 15 6.654829E-4 94.87009 1 GAGCGGC 1075 0.0 72.77891 9 ATTCGGA 225 0.0 65.345955 2 CGGGAGA 1375 0.0 63.801216 4 AGAGCGG 1245 0.0 62.46037 8 TCGGGAG 1540 0.0 59.120815 3 ATCGGGA 1830 0.0 57.536236 2 TCGGGAA 460 0.0 53.60502 3 TTGTGTT 28985 0.0 52.401936 1 GATCCGG 310 0.0 52.025536 1 ATCCGGA 270 0.0 50.941742 2 GTTCGGA 110 0.0 47.42852 2 CGGAAAG 640 0.0 47.419823 5 TCGGAAA 490 0.0 47.419823 4 AAGCGGC 310 0.0 44.35743 9 GATCGGG 2560 0.0 43.358593 1 GGTTCGG 235 0.0 42.38876 1 GGATCGG 620 0.0 42.079475 1 TGTGTTT 52830 0.0 41.44618 1 AAGCGTC 220 0.0 40.95067 9 >>END_MODULE