FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_30230-1.34200000035711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_30230-1.34200000035711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185036
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA314171.4378252806818743No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC216310.9899608061377478No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT120400.5510206696823301No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT114290.5230577436710425No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC104690.47912254077278366No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT57370.26255860315344914No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT56530.25871427289985155No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51620.23624324725084622No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT43630.19967634400531617Illumina Single End PCR Primer 1 (96% over 32bp)
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC30270.13853318663857253No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT29260.13391083716698488No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC28990.13267515958547135No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26990.12352199231500077No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA25310.11583333180780545No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23990.10979224140929486No Hit
GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT23220.10626827201016369No Hit
AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA22400.10251547342927073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG156.654829E-494.870091
GAGCGGC10750.072.778919
ATTCGGA2250.065.3459552
CGGGAGA13750.063.8012164
AGAGCGG12450.062.460378
TCGGGAG15400.059.1208153
ATCGGGA18300.057.5362362
TCGGGAA4600.053.605023
TTGTGTT289850.052.4019361
GATCCGG3100.052.0255361
ATCCGGA2700.050.9417422
GTTCGGA1100.047.428522
CGGAAAG6400.047.4198235
TCGGAAA4900.047.4198234
AAGCGGC3100.044.357439
GATCGGG25600.043.3585931
GGTTCGG2350.042.388761
GGATCGG6200.042.0794751
TGTGTTT528300.041.446181
AAGCGTC2200.040.950679
Produced by FastQC (version 0.11.2)