##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_53115004-1.3410000003588d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4562153 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.655307044722086 33.0 33.0 33.0 33.0 33.0 2 32.622503673156075 33.0 33.0 33.0 33.0 33.0 3 32.70171386185426 33.0 33.0 33.0 33.0 33.0 4 36.63632894381227 37.0 37.0 37.0 37.0 37.0 5 36.65326743754539 37.0 37.0 37.0 37.0 37.0 6 36.53497285163387 37.0 37.0 37.0 37.0 37.0 7 36.39148599356488 37.0 37.0 37.0 37.0 37.0 8 36.34902599715529 37.0 37.0 37.0 37.0 37.0 9 36.39568795697996 37.0 37.0 37.0 37.0 37.0 10-11 36.23885279603731 37.0 37.0 37.0 35.0 37.0 12-13 36.31211875182616 37.0 37.0 37.0 37.0 37.0 14-15 38.2004580951143 40.0 37.0 40.0 37.0 40.0 16-17 38.21448086024296 40.0 37.0 40.0 37.0 40.0 18-19 38.09153715362024 40.0 37.0 40.0 37.0 40.0 20-21 38.16029635568995 40.0 37.0 40.0 37.0 40.0 22-23 37.89989452348485 40.0 37.0 40.0 33.0 40.0 24-25 38.07650905175693 40.0 37.0 40.0 37.0 40.0 26-27 38.078048346909895 40.0 37.0 40.0 37.0 40.0 28-29 37.85698715058439 40.0 37.0 40.0 33.0 40.0 30-31 37.74494082070461 40.0 37.0 40.0 33.0 40.0 32-33 37.596915644872055 40.0 37.0 40.0 33.0 40.0 34-35 37.55301005906641 40.0 37.0 40.0 33.0 40.0 36-37 37.62655450178896 40.0 37.0 40.0 33.0 40.0 38-39 37.424134723232655 40.0 37.0 40.0 33.0 40.0 40-41 37.045452552774975 40.0 37.0 40.0 33.0 40.0 42-43 36.95034559340732 37.0 37.0 40.0 33.0 40.0 44-45 36.87845497509619 37.0 37.0 40.0 33.0 40.0 46-47 37.09019184582367 37.0 37.0 40.0 33.0 40.0 48-49 36.82297404317654 37.0 37.0 40.0 33.0 40.0 50-51 36.80592748643021 37.0 37.0 40.0 33.0 40.0 52-53 36.692145682093525 37.0 37.0 40.0 33.0 40.0 54-55 36.22071804693968 37.0 37.0 40.0 33.0 40.0 56-57 36.184698759554976 37.0 37.0 40.0 33.0 40.0 58-59 36.14400514406246 37.0 37.0 40.0 33.0 40.0 60-61 35.845399310369466 37.0 37.0 40.0 30.0 40.0 62-63 35.652710463678005 37.0 35.0 40.0 27.0 40.0 64-65 35.334773625522864 37.0 33.0 38.5 27.0 40.0 66-67 34.39416718378362 37.0 33.0 37.0 27.0 40.0 68-69 34.24243224635386 37.0 33.0 37.0 27.0 40.0 70-71 33.752982309010676 37.0 33.0 37.0 27.0 38.5 72-73 32.555041665634626 37.0 33.0 37.0 22.0 37.0 74-75 31.526155852291673 35.0 30.0 37.0 15.0 37.0 76-77 29.03542548879882 33.0 27.0 35.0 15.0 37.0 78-79 30.884141873365493 33.0 30.0 37.0 15.0 37.0 80-81 31.10077829480949 37.0 33.0 37.0 15.0 37.0 82-83 30.93977558402798 37.0 33.0 37.0 15.0 37.0 84-85 30.79166722378666 33.0 33.0 37.0 15.0 37.0 86-87 30.658346508764613 33.0 33.0 37.0 15.0 37.0 88-89 30.505189216582608 33.0 33.0 37.0 15.0 37.0 90-91 30.411194560989077 33.0 33.0 37.0 10.5 37.0 92-93 30.242133155113386 33.0 27.0 37.0 6.0 37.0 94-95 30.12557568761942 33.0 27.0 37.0 6.0 37.0 96-97 29.97620947828799 33.0 27.0 37.0 6.0 37.0 98-99 29.853999635698322 33.0 27.0 37.0 6.0 37.0 100-101 28.44203515313932 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16662016699152105 2306 2 0.2019522898281778 2306 3 0.1718817656706051 2306 4 0.16199807994347282 2306 5 0.12222340752369831 2306 6 0.1632768858173108 2306 7 0.1724280962529008 2306 8 0.08049146451504186 2306 9 0.11161371541576415 2306 10-11 0.20285321136754675 2306 12-13 0.17143852471948406 2306 14-15 0.3327407244223437 2306 16-17 0.4607553700093092 2306 18-19 0.5344395957224606 2306 20-21 0.44567230437309746 2306 22-23 0.3701891336878873 2306 24-25 0.4214634674777855 2306 26-27 0.4358820788477189 2306 28-29 0.46041773155995 2306 30-31 0.581712417589678 2306 32-33 0.601954020610826 2306 34-35 0.5458438996843427 2306 36-37 0.4817447173382945 2306 38-39 0.48721574249191946 2306 40-41 0.6034589348356221 2306 42-43 0.5722770528162826 2306 44-45 0.5606924759111891 2306 46-47 0.5073968561084428 2306 48-49 0.507080652826005 2306 50-51 0.48203488448375253 2306 52-53 0.6367532584037079 2306 54-55 0.8601016219314275 2306 56-57 0.6761602466766874 2306 58-59 0.46777072819700294 2306 60-61 0.5722959635792577 2306 62-63 0.5457753840356858 2306 64-65 0.44806948179545714 2306 66-67 0.45464694568966024 2306 68-69 0.5088887864308518 2306 70-71 0.30840385335998377 2306 72-73 -0.1875660933775407 2306 74-75 -0.020973110183270194 2306 76-77 -0.05604233398213765 2306 78-79 0.22360912748201756 2306 80-81 0.24784885578034377 2306 82-83 0.27321016467171333 2306 84-85 0.22719584138545557 2306 86-87 0.4341612318374608 2306 88-89 0.30076673157399725 2306 90-91 0.2676688484863057 2306 92-93 0.16695399584030213 2306 94-95 0.21864437579193563 2306 96-97 0.32525958097903995 2306 98-99 0.2818042334197912 2306 100-101 0.2666542266343832 2307 1 0.1244921653367328 2307 2 0.1961069373394082 2307 3 0.15077145897059552 2307 4 0.15505219905063683 2307 5 0.11607671428011912 2307 6 0.18384985635079687 2307 7 0.2056056663463579 2307 8 0.17578283892975577 2307 9 0.16673180714370517 2307 10-11 0.24274536804593794 2307 12-13 0.2014790482344111 2307 14-15 0.31871827195050173 2307 16-17 0.37763374305129815 2307 18-19 0.5018205167627201 2307 20-21 0.4933650026186527 2307 22-23 0.42920890653055466 2307 24-25 0.3969044310160257 2307 26-27 0.3408631333993313 2307 28-29 0.2345383900905773 2307 30-31 0.4070626498242049 2307 32-33 0.46519185885524905 2307 34-35 0.4944890415636536 2307 36-37 0.452290891580013 2307 38-39 0.3096933652377416 2307 40-41 0.5121357621546778 2307 42-43 0.5264302429853345 2307 44-45 0.45101783930650896 2307 46-47 0.3858587489608851 2307 48-49 0.4009419514589254 2307 50-51 0.5261545087715405 2307 52-53 0.5256483109620476 2307 54-55 0.6167468672350225 2307 56-57 0.6766625596197713 2307 58-59 0.5323161784687258 2307 60-61 0.41372608637208685 2307 62-63 0.47184629548569745 2307 64-65 0.3564273392203816 2307 66-67 0.3311190963506334 2307 68-69 0.1708791867768511 2307 70-71 0.11862757889073805 2307 72-73 -0.3171470839941364 2307 74-75 -0.17214650569653855 2307 76-77 -0.3193525692253729 2307 78-79 0.09293630550341803 2307 80-81 0.1325422968002954 2307 82-83 0.314161244985371 2307 84-85 0.19811293271849806 2307 86-87 0.13353786547103752 2307 88-89 0.02546045466017688 2307 90-91 -0.040004763277739386 2307 92-93 -0.06304304246429382 2307 94-95 0.03394670377854325 2307 96-97 0.2166243584287848 2307 98-99 -0.1276522719676727 2307 100-101 -0.10159827905395602 2201 1 0.10818152893263999 2201 2 0.17685905126909773 2201 3 0.13098952750641502 2201 4 0.14314743714587763 2201 5 0.11721156074207073 2201 6 0.08656458710290593 2201 7 0.1462598719538306 2201 8 0.15183980377176454 2201 9 0.15576162467819188 2201 10-11 0.2093897828211979 2201 12-13 0.1945364581142499 2201 14-15 0.2586270391957157 2201 16-17 0.29087361844070614 2201 18-19 0.3694885185428731 2201 20-21 0.3053142683062333 2201 22-23 0.33222403781671517 2201 24-25 0.3127922814833184 2201 26-27 0.23467710758713878 2201 28-29 0.32972085560015074 2201 30-31 0.4607008340698684 2201 32-33 0.42701873914007393 2201 34-35 0.3888148092539154 2201 36-37 0.32645644565210574 2201 38-39 0.32900800698229204 2201 40-41 0.4659408355859185 2201 42-43 0.3577609060917055 2201 44-45 0.38943572982720553 2201 46-47 0.3406095277770831 2201 48-49 0.3207461348145131 2201 50-51 0.2792920254604496 2201 52-53 0.4126977101609839 2201 54-55 0.6294749491219847 2201 56-57 0.5659593998511809 2201 58-59 0.38973718425422277 2201 60-61 0.40914219674147034 2201 62-63 0.3979922678933505 2201 64-65 0.38556619102278233 2201 66-67 0.32640169537155117 2201 68-69 0.35316668121388517 2201 70-71 0.30617764564640737 2201 72-73 -0.1491008000600047 2201 74-75 -0.008072184379226854 2201 76-77 -0.09325110060053987 2201 78-79 0.19734218000275305 2201 80-81 0.12986094388529779 2201 82-83 0.27022488539480705 2201 84-85 0.2710212549258877 2201 86-87 0.40388722016618317 2201 88-89 0.24101452675630597 2201 90-91 0.2265506439318763 2201 92-93 0.07223733136748223 2201 94-95 0.13474777186933196 2201 96-97 0.2370849725809876 2201 98-99 0.10540291272302582 2201 100-101 0.259438659085788 1101 1 0.08342921801899905 1101 2 0.19729978463306708 1101 3 0.12792952765233423 1101 4 0.14279268363117126 1101 5 0.11098796206405126 1101 6 0.08825791956076046 1101 7 0.1286341852458719 1101 8 0.08672146748367027 1101 9 0.15220150444502423 1101 10-11 0.12850424873932553 1101 12-13 0.10236465527329841 1101 14-15 0.26033167999182183 1101 16-17 0.25700706047999944 1101 18-19 0.3318548248018516 1101 20-21 0.3708792839555102 1101 22-23 0.21204230187735362 1101 24-25 0.2298811869091324 1101 26-27 0.26544016506996826 1101 28-29 0.3417058702280258 1101 30-31 0.36002862631745103 1101 32-33 0.31392049156772117 1101 34-35 0.4006056670096072 1101 36-37 0.4587489558843245 1101 38-39 0.42154878995704337 1101 40-41 0.47414658532874654 1101 42-43 0.38878223142894797 1101 44-45 0.5111047858363023 1101 46-47 0.38430750030146754 1101 48-49 0.2789664759615391 1101 50-51 0.29392136731516416 1101 52-53 0.2961551616263236 1101 54-55 0.4733726414914088 1101 56-57 0.41032383476257905 1101 58-59 0.31495258985877683 1101 60-61 0.34188439581199503 1101 62-63 0.3213204577567055 1101 64-65 0.1582259121810523 1101 66-67 0.3933113089182072 1101 68-69 0.1342606899833143 1101 70-71 0.10409727619205711 1101 72-73 -0.2460304038304244 1101 74-75 -0.0733385322237119 1101 76-77 -0.25823829664335207 1101 78-79 0.2434074254666463 1101 80-81 0.25430026917876347 1101 82-83 0.2941769489841448 1101 84-85 0.3307953070317886 1101 86-87 0.26782757415364955 1101 88-89 0.08425742986208462 1101 90-91 0.19053349538408426 1101 92-93 0.24820255275556136 1101 94-95 0.16251703799465744 1101 96-97 0.14497418004886597 1101 98-99 0.13984510157957075 1101 100-101 0.24767889926030406 2304 1 0.17120549143820085 2304 2 0.17392632151831577 2304 3 0.1470216675820737 2304 4 0.10359296173421484 2304 5 0.07161899982752118 2304 6 0.07219838355373298 2304 7 0.13827631053861467 2304 8 0.12742148212067406 2304 9 0.0896420416230086 2304 10-11 0.08006491911402946 2304 12-13 0.09963622655549642 2304 14-15 0.17641134187707053 2304 16-17 0.15531648670950915 2304 18-19 0.3221486626238672 2304 20-21 0.2127356217775258 2304 22-23 0.1851582452321594 2304 24-25 0.10659417358945689 2304 26-27 0.045773065391991 2304 28-29 0.18161365711327448 2304 30-31 0.2862101068502412 2304 32-33 0.29941613705951653 2304 34-35 0.2662837261431008 2304 36-37 0.3037934157789408 2304 38-39 0.24190245652612674 2304 40-41 0.3294964096847224 2304 42-43 0.2327952400647817 2304 44-45 0.16289278715363764 2304 46-47 0.24146179260240785 2304 48-49 0.18285168527067697 2304 50-51 0.16822153564069708 2304 52-53 0.17728669338861636 2304 54-55 0.3528872419012501 2304 56-57 0.36939740399560606 2304 58-59 0.29564775598875315 2304 60-61 0.2866960358881059 2304 62-63 0.19657431826606597 2304 64-65 0.05191324751477566 2304 66-67 0.08573071392407172 2304 68-69 0.03326079976083207 2304 70-71 -0.022915311629958524 2304 72-73 -0.30213357640067784 2304 74-75 -0.11105854466516263 2304 76-77 -0.07739584567731228 2304 78-79 0.036662054418638235 2304 80-81 0.18401978762138782 2304 82-83 0.10219894108016447 2304 84-85 0.23107615451422703 2304 86-87 0.14911462406582743 2304 88-89 0.1272993648737959 2304 90-91 0.03843445776698573 2304 92-93 0.07122061115720157 2304 94-95 0.14348207385531353 2304 96-97 0.12598266951022552 2304 98-99 0.1565495805022863 2304 100-101 0.2182630986162728 2202 1 0.11061934725830724 2202 2 0.17485638104212597 2202 3 0.13086343345509022 2202 4 0.11116157945908611 2202 5 0.1167516883195816 2202 6 0.15716242125214563 2202 7 0.11660799043279013 2202 8 0.10353341995136134 2202 9 0.08532449863358949 2202 10-11 0.1015472273150877 2202 12-13 0.15447563627771643 2202 14-15 0.17558668909929054 2202 16-17 0.1687589470269657 2202 18-19 0.31996198934344733 2202 20-21 0.28815311516377307 2202 22-23 0.20802634212368787 2202 24-25 0.21212596095718084 2202 26-27 0.07874574230997666 2202 28-29 0.20378627616089062 2202 30-31 0.25679439091108947 2202 32-33 0.19344928851455023 2202 34-35 0.33315293910606414 2202 36-37 0.3508482272868818 2202 38-39 0.3267259518964991 2202 40-41 0.4239243197376297 2202 42-43 0.31311001354361423 2202 44-45 0.3586403483308871 2202 46-47 0.3179509212399694 2202 48-49 0.36085887773099046 2202 50-51 0.3222455931803694 2202 52-53 0.2863527069468219 2202 54-55 0.45314972536685616 2202 56-57 0.4544885498289375 2202 58-59 0.48651560486441525 2202 60-61 0.4267681671711756 2202 62-63 0.3524636618507273 2202 64-65 0.2602620818401675 2202 66-67 0.08774274269391213 2202 68-69 0.29169953984020225 2202 70-71 0.16036841852876904 2202 72-73 0.13177122684006548 2202 74-75 0.14035411241155415 2202 76-77 0.07837155676978114 2202 78-79 0.41028659972286974 2202 80-81 0.46239073669546116 2202 82-83 0.522654050207148 2202 84-85 0.43956449776819184 2202 86-87 0.4269513056134464 2202 88-89 0.4899192392124192 2202 90-91 0.4025000579654012 2202 92-93 0.32582853939794276 2202 94-95 0.5197314538156306 2202 96-97 0.5384992039017611 2202 98-99 0.4527549376451425 2202 100-101 0.47635875007130224 1102 1 0.07566894958738146 1102 2 0.13470101948558977 1102 3 0.10873151412296522 1102 4 0.06782031841135705 1102 5 0.04496434046473041 1102 6 0.08346389897269546 1102 7 0.03317982215420301 1102 8 0.07568739077574804 1102 9 0.07207418984505409 1102 10-11 0.18695394206212512 1102 12-13 0.1414417838549653 1102 14-15 0.13187471270385487 1102 16-17 0.03242420943296764 1102 18-19 0.19615087593248148 1102 20-21 0.18106741602999676 1102 22-23 0.06890821241491807 1102 24-25 0.10789691088947251 1102 26-27 0.08173743985549464 1102 28-29 0.13315429797969358 1102 30-31 0.14207961952282488 1102 32-33 0.10110592513436245 1102 34-35 0.1411583072239324 1102 36-37 0.13084593420224877 1102 38-39 0.1590512749914481 1102 40-41 0.21880072991702804 1102 42-43 0.05813073924063161 1102 44-45 0.08899568174809502 1102 46-47 0.08512507662047142 1102 48-49 0.13360749477774192 1102 50-51 0.0912564822884292 1102 52-53 0.07367800298472105 1102 54-55 0.11635650613308712 1102 56-57 0.20997886408299848 1102 58-59 0.1417524284213627 1102 60-61 0.11275187341824022 1102 62-63 0.0928788048675031 1102 64-65 0.07482306774480207 1102 66-67 0.1396752245420103 1102 68-69 0.07081420624711399 1102 70-71 -0.08677537208216535 1102 72-73 -0.054070328453043714 1102 74-75 -0.04562414860505726 1102 76-77 -0.0952525248126257 1102 78-79 0.14477334109404083 1102 80-81 0.27230322068107427 1102 82-83 0.1963319753105388 1102 84-85 0.2201507968919536 1102 86-87 0.23887950568406424 1102 88-89 0.13039231812835084 1102 90-91 0.11277987516799826 1102 92-93 -0.05328474033538555 1102 94-95 -0.010251236439369649 1102 96-97 0.12959256559087962 1102 98-99 0.1821336131756972 1102 100-101 0.229792864135689 2305 1 0.09525912402137493 2305 2 0.16890400008974638 2305 3 0.08333679722982623 2305 4 0.14133299353461126 2305 5 0.06930659155941754 2305 6 0.03487496745783858 2305 7 0.052614006233326904 2305 8 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-1.2431410976872392 1213 96-97 -1.5346651268423592 1213 98-99 -1.5208487857591102 1213 100-101 -1.5005712003958855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 97.0 9 266.0 10 217.0 11 88.0 12 77.0 13 141.0 14 324.0 15 799.0 16 1497.0 17 2561.0 18 3934.0 19 5919.0 20 8254.0 21 11577.0 22 16104.0 23 22394.0 24 30670.0 25 41933.0 26 59134.0 27 88685.0 28 153387.0 29 240844.0 30 190851.0 31 103022.0 32 116124.0 33 163467.0 34 253445.0 35 446629.0 36 902913.0 37 1377157.0 38 319579.0 39 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25415364080228 23.09650409217043 19.874315044434297 13.775027222592994 2 26.694194605047223 32.346964251308535 28.315775468293154 12.643065675351089 3 32.773889697971896 17.296297400403148 34.34840858097557 15.581404320649387 4 26.03864829612092 17.174026942005636 28.726513432639507 28.060811329233935 5 40.528167292942605 16.039334936816015 27.590240835850967 15.84225693439041 6 35.516410782365256 17.09591940471966 31.540349479730295 15.847320333184792 7 28.691102643861356 29.71901643807211 24.451437731264164 17.13844318680237 8 25.19619574354477 35.72396629398445 22.306375958894847 16.773462003575943 9 37.98040092035493 21.80444189399172 22.683873162517784 17.53128402313557 10-11 27.154722781107953 31.656851490951748 25.73921786489844 15.449207863041858 12-13 20.719055235543394 30.252865259012573 25.69758182156539 23.330497683878644 14-15 20.301544029759633 30.730698860822947 26.066771544049487 22.900985565367932 16-17 25.95610011325793 24.91824583699845 26.598132504543358 22.527521545200262 18-19 18.899114080566783 25.10916446686466 33.01111339317204 22.980608059396516 20-21 25.174355745451297 25.220770239486733 32.74056489781961 16.864309117242353 22-23 18.66684699112063 37.70310338531397 26.536332564470193 17.093717059095205 24-25 18.848107461542828 24.599043477936846 32.94772227060337 23.60512678991695 26-27 18.760955627748565 24.788537341908523 26.444597539801933 30.005909490540976 28-29 24.48262914461659 31.58950390309137 26.150712174712247 17.777154777579796 30-31 17.652454882596004 26.253185721741467 32.13469605249977 23.959663343162756 32-33 17.42413066812972 32.33306730396811 26.538873203068814 23.703928824833362 34-35 17.898555791530885 32.2643607086391 33.10043525502104 16.736648244808976 36-37 17.79857559752602 26.581952461392156 32.892544447777446 22.72692749330438 38-39 24.802236004231855 32.35903071557594 25.76810311730441 17.070630162887806 40-41 18.347955371591944 38.18031661202012 25.677906702097868 17.793821314290067 42-43 18.38979060655839 31.97922242578639 32.356970596887265 17.27401637076795 44-45 17.805791192652507 25.756954034326295 32.82608886258521 23.611165910435982 46-47 24.334291300067754 25.66337424511074 26.506901547162677 23.49543290765883 48-49 18.45898846492008 32.33821723249709 31.922445367675607 17.280348934907234 50-51 24.73323286845494 25.86474504753815 31.421859330903914 17.980162753102995 52-53 18.272297099770917 25.78389776398451 25.440895392717 30.502909743527574 54-55 24.818937158792536 25.928187898199845 31.523872449758727 17.729002493248892 56-57 18.45573421729069 25.811660032491723 31.659238780636123 24.07336696958146 58-59 18.388733155222546 25.862962558586478 32.05273999902658 23.6955642871644 60-61 24.32295304482701 26.166084543013657 31.81607340996046 17.694889002198874 62-63 17.92802817074304 26.261295741945894 31.910394274661236 23.90028181264983 64-65 24.515740661781454 26.200424538714767 31.696607175857373 17.587227623646406 66-67 18.870004370896254 37.93633181713187 25.238401310983843 17.955262500988027 68-69 18.880047446697834 38.1209660577418 25.252441537128302 17.746544958432068 70-71 18.36938877020057 38.86550874569134 25.331736805817222 17.433365678290862 72-73 18.22809856034296 38.81579279147524 25.15123097375228 17.804877674429516 74-75 18.98482352181436 37.412960916958774 25.061828735963747 18.540386825263116 76-77 19.340660304347733 35.893563797484454 25.640550904032466 19.125224994135355 78-79 19.03727817785231 34.853243892012756 26.57628270472307 19.533195225411855 80-81 18.554192673099777 34.84997045348394 26.39518363836599 20.20065323505029 82-83 18.037907428904255 34.22161116053701 26.738572853596814 21.001908556961926 84-85 18.42527692577669 33.53528161377696 26.988281533871593 21.05115992657476 86-87 18.473457706582888 33.40069249250158 27.64549698977794 20.480352811137593 88-89 18.8305830346706 33.55263157894737 27.321622275753025 20.295163110629 90-91 19.149120427084103 32.99128237592938 26.87255716059228 20.98704003639423 92-93 18.754756798939805 33.35267577581671 26.673513277259985 21.219054147983503 94-95 18.51555731984674 33.63663180242083 26.496987667750826 21.3508232099816 96-97 18.97079270063661 33.97560980155869 26.18385723531628 20.869740262488417 98-99 19.504118459006726 32.919276449299595 26.095988106736772 21.480616984956907 100-101 19.50947347671009 33.08648071608054 25.608685007954634 21.79536079925474 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 85.0 1 62.0 2 101.5 3 292.5 4 322.0 5 199.0 6 164.5 7 165.0 8 174.0 9 172.5 10 165.5 11 149.5 12 151.5 13 171.5 14 173.0 15 177.5 16 195.0 17 248.5 18 332.5 19 435.0 20 591.5 21 818.5 22 1147.0 23 1668.5 24 2471.0 25 3544.0 26 5069.0 27 7179.0 28 9742.0 29 12785.0 30 16581.0 31 21446.5 32 29269.5 33 46564.0 34 75863.5 35 124832.0 36 190637.5 37 267657.5 38 347928.0 39 387106.0 40 387408.5 41 356090.0 42 313391.5 43 302091.0 44 283721.0 45 252838.0 46 239310.0 47 212001.5 48 161611.0 49 118140.5 50 89119.5 51 71435.5 52 58026.0 53 44990.5 54 34209.5 55 24465.5 56 16433.5 57 11456.5 58 8348.5 59 5943.0 60 4229.5 61 3220.5 62 2340.5 63 1501.0 64 989.5 65 661.0 66 417.5 67 296.5 68 208.0 69 130.5 70 91.5 71 68.5 72 44.5 73 28.5 74 20.0 75 15.5 76 13.0 77 14.0 78 12.0 79 7.0 80 4.5 81 3.0 82 2.5 83 2.5 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15591322781151792 2 0.0 3 0.001337087993322451 4 0.011025496076085129 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0959737650184024E-5 22-23 1.315168518022083E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.466245213608575E-4 38-39 0.0018960346134818363 40-41 0.005710023315745877 42-43 0.002608417560743798 44-45 0.0011617321909195065 46-47 0.0013151685180220832 48-49 0.0034194381468574155 50-51 6.137453084103054E-4 52-53 0.014653169238296041 54-55 0.027103431208905092 56-57 0.02848435815282828 58-59 0.020757743109448543 60-61 0.030895500435868764 62-63 0.0056333051521945884 64-65 0.010872059748982553 66-67 0.028572036054029755 68-69 0.027300706486608404 70-71 0.031158534139473185 72-73 0.030994138074720422 74-75 0.03887418944520274 76-77 0.02023167570223971 78-79 0.011025496076085129 80-81 0.015376511923208188 82-83 0.007222467111471273 84-85 0.011102214239636417 86-87 0.03344911930836164 88-89 0.015540907987960949 90-91 0.021711240285014553 92-93 0.048935228608071674 94-95 0.05617961519484332 96-97 0.0402770358644263 98-99 0.034808126776984465 100-101 0.035169798119440536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4562153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.317178381480886 #Duplication Level Percentage of deduplicated Percentage of total 1 87.33929049682814 29.098987211948014 2 5.6297074591661955 3.751319353051873 3 1.93827600336872 1.937336620703385 4 1.0847381292086258 1.445616549921506 5 0.6818770529567768 1.1359109703799712 6 0.46798257922636577 0.9355115442906203 7 0.2598680083004012 0.6060648151729235 8 0.23800822727956883 0.6343810051626753 9 0.22102865602370925 0.6627646044144812 >10 1.6481327309383806 10.826657907480977 >50 0.21774705969783936 5.128649884582572 >100 0.22012391872036824 14.956279835750117 >500 0.0364190810495965 8.490909476541104 >1k 0.01640220113233155 8.562937510424371 >5k 3.31996760035988E-4 0.7720714194754497 >10k+ 6.639934291872935E-5 11.054601290699868 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 499702 10.953205646544516 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8650 0.18960346134818362 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 6914 0.1515512522267447 TruSeq Adapter, Index 13 (97% over 38bp) TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6835 0.14981961367801563 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6466 0.14173132729217983 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6035 0.13228403343772116 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4907 0.10755886529890601 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 4811 0.1054545956700707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005085318269685388 0.0 0.0 2 0.005567546726293485 0.0 0.0 3 0.006400486787707471 0.0 0.0 4 0.006685439966612255 0.0 0.0 5 0.0074307021268247685 0.0 0.0 6 0.008044447435235075 0.0 0.0 7 0.009030823823751637 0.0 0.0 8 0.009995280736967831 0.0 0.0 9 0.011003576600784762 0.0 2.1919475300368048E-5 10-11 0.016099854608120332 0.0 2.1919475300368048E-5 12-13 0.020790622322399095 0.0 2.1919475300368048E-5 14-15 0.025843061379133933 0.0 2.1919475300368048E-5 16-17 0.02978856693320018 0.0 2.1919475300368048E-5 18-19 0.03475332808873355 0.0 2.1919475300368048E-5 20-21 0.04030991507737684 0.0 2.1919475300368048E-5 22-23 0.0456911462636172 0.0 2.1919475300368048E-5 24-25 0.05134637089111216 0.0 2.1919475300368048E-5 26-27 0.05785645505532147 0.0 4.3838950600736096E-5 28-29 0.0656597882622525 0.0 4.3838950600736096E-5 30-31 0.10255026519277192 0.0 4.3838950600736096E-5 32-33 0.11196467983427999 0.0 4.3838950600736096E-5 34-35 0.11966937540235936 0.0 4.3838950600736096E-5 36-37 0.12769190336229408 0.0 4.3838950600736096E-5 38-39 0.13770910357456229 0.0 5.479868825092012E-5 40-41 0.1481318140798873 0.0 8.767790120147219E-5 42-43 0.1585545245852123 0.0 1.2055711415202428E-4 44-45 0.1697772959390007 0.0 1.315168518022083E-4 46-47 0.18082471149038623 0.0 1.315168518022083E-4 48-49 0.19365856427875172 0.0 1.315168518022083E-4 50-51 0.20731439739088103 0.0 1.315168518022083E-4 52-53 0.22060855916055425 0.0 1.315168518022083E-4 54-55 0.23640154111446943 0.0 1.315168518022083E-4 56-57 0.25337817473460444 0.0 1.315168518022083E-4 58-59 0.27107815103965166 0.0 1.315168518022083E-4 60-61 0.2903782490416258 0.0 1.315168518022083E-4 62-63 0.311179831101675 0.0 1.5343632710257635E-4 64-65 0.33421719964236185 0.0 1.5343632710257635E-4 66-67 0.35842726011161835 0.0 1.5343632710257635E-4 68-69 0.3849388654874135 0.0 1.5343632710257635E-4 70-71 0.41363145865559525 0.0 1.5343632710257635E-4 72-73 0.446192839214292 0.0 1.6439606475276037E-4 74-75 0.48194350342919234 0.0 1.9727527770331245E-4 76-77 0.5227356469631772 0.0 1.9727527770331245E-4 78-79 0.5666841949404152 0.0 1.9727527770331245E-4 80-81 0.6126602943829371 0.0 1.9727527770331245E-4 82-83 0.6658479011992802 0.0 1.9727527770331245E-4 84-85 0.7249428066090725 0.0 1.9727527770331245E-4 86-87 0.7894518224180557 0.0 2.191947530036805E-4 88-89 0.8614025000915138 0.0 2.191947530036805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 63180 0.0 86.94387 1 TCGGAAG 63310 0.0 86.51504 3 CGGAAGA 63160 0.0 86.09545 4 ATCGGAA 63715 0.0 85.912926 2 GAAGAGC 63545 0.0 85.42527 6 GGAAGAG 65555 0.0 83.00885 5 TCGGACG 180 0.0 68.59974 3 AGAGCAC 80525 0.0 67.37069 8 GAGCACA 80750 0.0 67.21825 9 AAGAGCA 81285 0.0 66.68821 7 ATCGGAC 210 0.0 61.0613 2 CGCGCGG 35 2.7704693E-4 54.35834 1 GCGCACA 475 0.0 49.987324 9 GCCGTCT 58635 0.0 46.21062 50-51 ATGCCGT 58540 0.0 46.1469 48-49 CGTCTTC 58715 0.0 46.14437 52-53 TCTCGTA 58240 0.0 46.097195 42-43 TCGTATG 59350 0.0 46.087765 44-45 AATCTCG 57830 0.0 46.030575 40-41 TTGTGTT 39405 0.0 46.024567 1 >>END_MODULE