FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562153
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT49970210.953205646544516TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC86500.18960346134818362No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT69140.1515512522267447TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG68350.14981961367801563No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA64660.14173132729217983No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC60350.13228403343772116No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG49070.10755886529890601No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT48110.1054545956700707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA631800.086.943871
TCGGAAG633100.086.515043
CGGAAGA631600.086.095454
ATCGGAA637150.085.9129262
GAAGAGC635450.085.425276
GGAAGAG655550.083.008855
TCGGACG1800.068.599743
AGAGCAC805250.067.370698
GAGCACA807500.067.218259
AAGAGCA812850.066.688217
ATCGGAC2100.061.06132
CGCGCGG352.7704693E-454.358341
GCGCACA4750.049.9873249
GCCGTCT586350.046.2106250-51
ATGCCGT585400.046.146948-49
CGTCTTC587150.046.1443752-53
TCTCGTA582400.046.09719542-43
TCGTATG593500.046.08776544-45
AATCTCG578300.046.03057540-41
TTGTGTT394050.046.0245671
Produced by FastQC (version 0.11.2)