##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5145300 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68880998192525 33.0 33.0 33.0 33.0 33.0 2 32.66405923852837 33.0 33.0 33.0 33.0 33.0 3 32.739787573124985 33.0 33.0 33.0 33.0 33.0 4 36.68620313684333 37.0 37.0 37.0 37.0 37.0 5 36.70607097739685 37.0 37.0 37.0 37.0 37.0 6 36.598382018541194 37.0 37.0 37.0 37.0 37.0 7 36.49128602802558 37.0 37.0 37.0 37.0 37.0 8 36.4392414436476 37.0 37.0 37.0 37.0 37.0 9 36.51398052591686 37.0 37.0 37.0 37.0 37.0 10-11 36.362610634948396 37.0 37.0 37.0 37.0 37.0 12-13 36.403180475385305 37.0 37.0 37.0 37.0 37.0 14-15 38.350463821351525 40.0 37.0 40.0 37.0 40.0 16-17 38.36616028219929 40.0 37.0 40.0 37.0 40.0 18-19 38.25156161934193 40.0 37.0 40.0 37.0 40.0 20-21 38.327120576059706 40.0 37.0 40.0 37.0 40.0 22-23 38.15887975433891 40.0 37.0 40.0 37.0 40.0 24-25 38.237969894855496 40.0 37.0 40.0 37.0 40.0 26-27 38.21253201951295 40.0 37.0 40.0 37.0 40.0 28-29 38.03224544730142 40.0 37.0 40.0 37.0 40.0 30-31 37.99767788078441 40.0 37.0 40.0 37.0 40.0 32-33 37.90111616426641 40.0 37.0 40.0 33.0 40.0 34-35 37.74237644063514 40.0 37.0 40.0 33.0 40.0 36-37 37.43227936174762 40.0 37.0 40.0 33.0 40.0 38-39 37.65142304627524 40.0 37.0 40.0 33.0 40.0 40-41 37.363475987794686 40.0 37.0 40.0 33.0 40.0 42-43 37.26897236312752 40.0 37.0 40.0 33.0 40.0 44-45 37.35417993119934 40.0 37.0 40.0 33.0 40.0 46-47 37.3893386197112 40.0 37.0 40.0 33.0 40.0 48-49 37.1312243212252 40.0 37.0 40.0 33.0 40.0 50-51 37.08663411268536 37.0 37.0 40.0 33.0 40.0 52-53 37.00648174061765 37.0 37.0 40.0 33.0 40.0 54-55 36.56767982430568 37.0 37.0 40.0 33.0 40.0 56-57 36.536065244009094 37.0 37.0 40.0 33.0 40.0 58-59 36.35549336287485 37.0 37.0 40.0 33.0 40.0 60-61 36.08454434143782 37.0 37.0 40.0 33.0 40.0 62-63 35.91357345538647 37.0 37.0 40.0 33.0 40.0 64-65 35.6952127184032 37.0 35.0 40.0 33.0 40.0 66-67 34.98663420986142 37.0 33.0 37.0 27.0 40.0 68-69 34.86728130526889 37.0 33.0 37.0 27.0 40.0 70-71 34.47844372534158 37.0 33.0 37.0 27.0 40.0 72-73 33.657949682234275 37.0 33.0 37.0 24.5 40.0 74-75 32.77842186072726 37.0 33.0 37.0 22.0 38.5 76-77 30.293556255223216 33.0 30.0 35.0 22.0 37.0 78-79 32.29260256933512 35.0 33.0 37.0 22.0 37.0 80-81 32.503189998639535 37.0 33.0 37.0 18.5 37.0 82-83 32.34820243717567 37.0 33.0 37.0 15.0 37.0 84-85 32.210910636891924 37.0 33.0 37.0 15.0 37.0 86-87 32.07203068820088 37.0 33.0 37.0 15.0 37.0 88-89 31.914817211824385 37.0 33.0 37.0 15.0 37.0 90-91 31.79982469438128 37.0 33.0 37.0 15.0 37.0 92-93 31.629470390453424 37.0 33.0 37.0 15.0 37.0 94-95 31.521000816278935 33.0 33.0 37.0 15.0 37.0 96-97 31.384695741744892 33.0 33.0 37.0 15.0 37.0 98-99 31.25709288088158 33.0 33.0 37.0 15.0 37.0 100-101 29.850206596311196 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11312621211008889 2306 2 0.1810899167546154 2306 3 0.1559606188051319 2306 4 0.1206902039883957 2306 5 0.13181929271313209 2306 6 0.17381708166080045 2306 7 0.15160916404315827 2306 8 0.17271170402489133 2306 9 0.18159182579402255 2306 10-11 0.24532630957305912 2306 12-13 0.2139295039123965 2306 14-15 0.3623813740487307 2306 16-17 0.37533642625397334 2306 18-19 0.5032299188244096 2306 20-21 0.49999854742696925 2306 22-23 0.43760663195168803 2306 24-25 0.46982451020922156 2306 26-27 0.45095554564949936 2306 28-29 0.34424347095902874 2306 30-31 0.5360433114175933 2306 32-33 0.5052506653073792 2306 34-35 0.5866173218275534 2306 36-37 0.4849301294840629 2306 38-39 0.5482061074681326 2306 40-41 0.687423539876292 2306 42-43 0.5145427528836848 2306 44-45 0.579715243305138 2306 46-47 0.45836504483320795 2306 48-49 0.3946631957515834 2306 50-51 0.43374705857092977 2306 52-53 0.4851552660485794 2306 54-55 0.6480688687022251 2306 56-57 0.5703066271118971 2306 58-59 0.3763808872682688 2306 60-61 0.48291005618060723 2306 62-63 0.45837326846093873 2306 64-65 0.4175095488063576 2306 66-67 0.4160019611704584 2306 68-69 0.3170553874033999 2306 70-71 0.25510677940306437 2306 72-73 -0.10051538801928217 2306 74-75 -0.04569998028723177 2306 76-77 -0.18377497125822018 2306 78-79 0.07287733942569474 2306 80-81 0.11210535002074806 2306 82-83 0.16219294587413913 2306 84-85 0.11014481028647793 2306 86-87 0.07279729998094098 2306 88-89 0.14560514245806644 2306 90-91 0.08088068771117563 2306 92-93 -0.09793399430945371 2306 94-95 0.027720156904457838 2306 96-97 0.12199715498176644 2306 98-99 0.07916110485053096 2306 100-101 0.18326891688529656 2307 1 0.12159826982517075 2307 2 0.198301533607669 2307 3 0.13029678764328878 2307 4 0.1478809006611641 2307 5 0.12716609084877462 2307 6 0.1414272587132075 2307 7 0.1407491292787384 2307 8 0.12049370344035992 2307 9 0.15982130171273923 2307 10-11 0.190657725223204 2307 12-13 0.17510826377641564 2307 14-15 0.2550278537669257 2307 16-17 0.3307900557514287 2307 18-19 0.42985300178215624 2307 20-21 0.3748083429937381 2307 22-23 0.3573816631012221 2307 24-25 0.37508070550525474 2307 26-27 0.34990692161283476 2307 28-29 0.2705299851339902 2307 30-31 0.3659195593217248 2307 32-33 0.3915637142698358 2307 34-35 0.3892452656507217 2307 36-37 0.4007623988621489 2307 38-39 0.38919597041018505 2307 40-41 0.49612441206321733 2307 42-43 0.43134358046140875 2307 44-45 0.39211852592754326 2307 46-47 0.3064923810481943 2307 48-49 0.4241889537235579 2307 50-51 0.39558034319305335 2307 52-53 0.3823451935970681 2307 54-55 0.48865509522470063 2307 56-57 0.5417505041135371 2307 58-59 0.3521558568878618 2307 60-61 0.29374701721676644 2307 62-63 0.23001281067817558 2307 64-65 0.18708376160766704 2307 66-67 0.297390615049153 2307 68-69 0.1384555012337998 2307 70-71 0.07209719611459775 2307 72-73 -0.14498569213102286 2307 74-75 -0.11563998859376312 2307 76-77 -0.20844024837369446 2307 78-79 -0.03338379364819133 2307 80-81 2.3037886291632503E-4 2307 82-83 0.0821683338973358 2307 84-85 0.13660330064935522 2307 86-87 0.13234874670370544 2307 88-89 0.10099762657564781 2307 90-91 0.012541688495680603 2307 92-93 -0.00884479127602944 2307 94-95 0.047428043504776696 2307 96-97 3.016205173089759E-4 2307 98-99 0.03573734813992502 2307 100-101 -0.05157025922877878 2201 1 0.12057738499476756 2201 2 0.189837491334103 2201 3 0.13219977907009195 2201 4 0.15388772381084692 2201 5 0.1289263545137942 2201 6 0.14128909333694395 2201 7 0.18808558137709497 2201 8 0.13440135258610297 2201 9 0.10521404139119284 2201 10-11 0.1758292913471493 2201 12-13 0.20163928459314917 2201 14-15 0.3133655451998507 2201 16-17 0.28211420302795176 2201 18-19 0.3005244895964978 2201 20-21 0.2749862760288124 2201 22-23 0.3126574490912972 2201 24-25 0.3152804290383102 2201 26-27 0.26946382545545333 2201 28-29 0.3260850640224007 2201 30-31 0.45817865515224554 2201 32-33 0.49529652871693486 2201 34-35 0.42881592474518726 2201 36-37 0.39893724037032285 2201 38-39 0.4898444420110337 2201 40-41 0.5705546586363255 2201 42-43 0.4077421899552576 2201 44-45 0.40662503924357907 2201 46-47 0.3890155845237757 2201 48-49 0.43227540752429405 2201 50-51 0.4372159243524436 2201 52-53 0.4626762729757701 2201 54-55 0.6107337044461758 2201 56-57 0.5012977043750269 2201 58-59 0.43739834427320545 2201 60-61 0.4237195067130699 2201 62-63 0.48023919352360167 2201 64-65 0.3168614985282616 2201 66-67 0.3949094604251471 2201 68-69 0.4463668139070194 2201 70-71 0.3256404672769122 2201 72-73 0.007454094594478988 2201 74-75 0.06879894013087551 2201 76-77 -0.0241426514570513 2201 78-79 0.2543243907266657 2201 80-81 0.26310352411700677 2201 82-83 0.3174303986832143 2201 84-85 0.3330536285282548 2201 86-87 0.37208365167670365 2201 88-89 0.35317245446476164 2201 90-91 0.4288222547762466 2201 92-93 0.30653555505670127 2201 94-95 0.27811464847709644 2201 96-97 0.35137844564175325 2201 98-99 0.2075751383110429 2201 100-101 0.25446686633215876 1101 1 0.09221887616617863 1101 2 0.12973295075948243 1101 3 0.10113664319070637 1101 4 0.12242743007217882 1101 5 0.07182044563151635 1101 6 0.12656136571607135 1101 7 0.13241483207140448 1101 8 0.16362462323318994 1101 9 0.13271228113724476 1101 10-11 0.16454380816777814 1101 12-13 0.16556809593382127 1101 14-15 0.22254407897930406 1101 16-17 0.2418602771854168 1101 18-19 0.2749024163893452 1101 20-21 0.29936862367384265 1101 22-23 0.21989152674517953 1101 24-25 0.19487546460101157 1101 26-27 0.25391509526437517 1101 28-29 0.28720384872654137 1101 30-31 0.354490262692714 1101 32-33 0.3111550959510865 1101 34-35 0.25265583742078235 1101 36-37 0.37254196730362565 1101 38-39 0.37303321707196346 1101 40-41 0.4928509031763042 1101 42-43 0.35058578272072793 1101 44-45 0.3697762478434683 1101 46-47 0.3363417596707521 1101 48-49 0.2714019441747766 1101 50-51 0.3110996142472757 1101 52-53 0.3321443222549689 1101 54-55 0.45494610806350977 1101 56-57 0.4474482203747314 1101 58-59 0.4614785104972441 1101 60-61 0.4242099337709888 1101 62-63 0.4006675381588565 1101 64-65 0.28589369850605095 1101 66-67 0.505334800318586 1101 68-69 0.3439714308581472 1101 70-71 0.2841248014660209 1101 72-73 0.11625153653844933 1101 74-75 0.1749188012945524 1101 76-77 0.03649922033895692 1101 78-79 0.5030117031324721 1101 80-81 0.5909825861343805 1101 82-83 0.627869198813606 1101 84-85 0.5940494200926238 1101 86-87 0.6334601576867271 1101 88-89 0.4953128262260833 1101 90-91 0.5542722921378349 1101 92-93 0.349235685777348 1101 94-95 0.3722920087539059 1101 96-97 0.5053342637168576 1101 98-99 0.46994157376354906 1101 100-101 0.44386920305804267 2304 1 0.09691522537881525 2304 2 0.1596910205001123 2304 3 0.0850997745172819 2304 4 0.10095031964774392 2304 5 0.09716203125891099 2304 6 0.07135910681073909 2304 7 0.095564663975928 2304 8 0.10063172949554655 2304 9 0.09292885624041247 2304 10-11 0.10254863297210193 2304 12-13 0.07024266080430408 2304 14-15 0.15166017794363285 2304 16-17 0.17885639929114205 2304 18-19 0.2957925507986232 2304 20-21 0.24036260002728227 2304 22-23 0.22029355769950598 2304 24-25 0.10249708410506742 2304 26-27 0.12343987195051653 2304 28-29 0.18355745502536536 2304 30-31 0.28734281462072175 2304 32-33 0.2901899121563858 2304 34-35 0.28125906825624014 2304 36-37 0.22648125381176953 2304 38-39 0.16457935561605552 2304 40-41 0.3186337921761506 2304 42-43 0.2561949748690182 2304 44-45 0.23466148992021374 2304 46-47 0.24067646499559459 2304 48-49 0.20635517547557924 2304 50-51 0.14192093048038856 2304 52-53 0.15649889212946988 2304 54-55 0.39575113527932615 2304 56-57 0.37428940851634707 2304 58-59 0.27547875420484047 2304 60-61 0.21103656438797458 2304 62-63 0.1338243349926529 2304 64-65 0.12983431826354774 2304 66-67 0.2071681372231282 2304 68-69 0.19651194183461484 2304 70-71 0.1450150570797959 2304 72-73 -0.006647540597604973 2304 74-75 0.16630873755614317 2304 76-77 -0.07331415505233352 2304 78-79 0.21490355150306328 2304 80-81 0.35888259502991815 2304 82-83 0.1805976416757602 2304 84-85 0.20354544534297503 2304 86-87 0.30880890736504796 2304 88-89 0.30981357953361055 2304 90-91 0.2640412763858002 2304 92-93 0.2774011952658171 2304 94-95 0.24743677777386708 2304 96-97 0.20332915191761813 2304 98-99 0.2966476189022629 2304 100-101 0.3447301012135924 2202 1 0.10194335128624488 2202 2 0.12627640654739025 2202 3 0.11578389161896752 2202 4 0.09058479084308857 2202 5 0.08996236691393733 2202 6 0.0915156071671106 2202 7 0.09875392669478344 2202 8 0.05983156502777831 2202 9 0.040141683606059075 2202 10-11 0.14138149380725906 2202 12-13 0.13383017989288248 2202 14-15 0.13907656416449754 2202 16-17 0.140801930543617 2202 18-19 0.2266441954362648 2202 20-21 0.1350986816628108 2202 22-23 0.1660634424200822 2202 24-25 0.17909438526586285 2202 26-27 0.05818792669524697 2202 28-29 0.13595854638416682 2202 30-31 0.19154239217214553 2202 32-33 0.22525309345569156 2202 34-35 0.2242572591908285 2202 36-37 0.24249671226166214 2202 38-39 0.23253919940367496 2202 40-41 0.3478346196345825 2202 42-43 0.17004483443946583 2202 44-45 0.17956033944580696 2202 46-47 0.13348698989253904 2202 48-49 0.18752232663953095 2202 50-51 0.22218450887015706 2202 52-53 0.2738534109532651 2202 54-55 0.3376753061210138 2202 56-57 0.42655362005900344 2202 58-59 0.33631737781124116 2202 60-61 0.20817607720821485 2202 62-63 0.13509439050052663 2202 64-65 0.23064346806280156 2202 66-67 0.19639538871560802 2202 68-69 0.10743616907210907 2202 70-71 0.05350851516651289 2202 72-73 -0.0111958522032225 2202 74-75 -0.035729880770652755 2202 76-77 -0.06251840825589738 2202 78-79 0.1837555689129644 2202 80-81 0.22463533302622096 2202 82-83 0.10786550294687913 2202 84-85 0.142098716979568 2202 86-87 0.16884782046991376 2202 88-89 0.1359513148860998 2202 90-91 0.002316898679502799 2202 92-93 -0.0039767202172704685 2202 94-95 0.10524735666679419 2202 96-97 0.13275590256737146 2202 98-99 0.12099633735761017 2202 100-101 0.08498842599422929 1102 1 0.10342602978852966 1102 2 0.1504740026869129 1102 3 0.10140764426262194 1102 4 0.07374324605741123 1102 5 0.058018129327926715 1102 6 0.01829281070587996 1102 7 0.04299374677759715 1102 8 0.059278632437148815 1102 9 0.08362340890467834 1102 10-11 0.16258282829237203 1102 12-13 0.14159245983687896 1102 14-15 0.07598639705597776 1102 16-17 0.10056444878853199 1102 18-19 0.25063346444820667 1102 20-21 0.1973197951238319 1102 22-23 0.05012502086576376 1102 24-25 0.11568995882637267 1102 26-27 0.010592904205594778 1102 28-29 0.156383168826153 1102 30-31 0.20910554191448938 1102 32-33 0.21585081917134374 1102 34-35 0.17299240261819904 1102 36-37 0.12190360362622954 1102 38-39 0.07916333183941049 1102 40-41 0.22003588709758048 1102 42-43 0.1264432139932481 1102 44-45 0.12269857631692105 1102 46-47 -0.03220493915336675 1102 48-49 0.126431280796389 1102 50-51 0.10475521721961911 1102 52-53 0.08529297188383111 1102 54-55 0.16532882403826932 1102 56-57 0.22826733874477867 1102 58-59 0.1467974052158496 1102 60-61 0.15766708134869845 1102 62-63 0.05865229762274282 1102 64-65 0.056778562340603855 1102 66-67 0.07582797189753165 1102 68-69 0.019599569030404496 1102 70-71 0.023773215354175647 1102 72-73 0.0015139643050758878 1102 74-75 0.019090313742466947 1102 76-77 -0.10891894741171626 1102 78-79 0.17304543218781987 1102 80-81 0.35781054377358856 1102 82-83 0.23097845758034197 1102 84-85 0.28589097815737574 1102 86-87 0.31371727625019474 1102 88-89 0.2640639344089024 1102 90-91 0.25404420796430216 1102 92-93 0.18334771903088765 1102 94-95 0.08770003159456152 1102 96-97 0.25287147851089387 1102 98-99 0.23777067383850436 1102 100-101 0.22324707929618626 2305 1 0.0733595191430183 2305 2 0.09822827454399174 2305 3 0.04506502110211841 2305 4 0.026154213745819277 2305 5 -0.01135638022520169 2305 6 -0.044114025993891914 2305 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-1.7701187163958245 1213 100-101 -1.7148533901426184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 28.0 10 17.0 11 28.0 12 27.0 13 69.0 14 246.0 15 622.0 16 1309.0 17 2459.0 18 3917.0 19 5683.0 20 8023.0 21 11071.0 22 14978.0 23 20320.0 24 27583.0 25 36618.0 26 52327.0 27 81246.0 28 145942.0 29 202095.0 30 128362.0 31 90317.0 32 116499.0 33 166479.0 34 258186.0 35 461685.0 36 972158.0 37 1726685.0 38 609952.0 39 366.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.738639299222534 24.384398952735054 23.051664953865327 12.825296794177085 2 26.955804326278347 29.674537927817617 32.00684119487688 11.362816551027151 3 34.59383135592973 18.135879647400547 32.509953422743266 14.760335573926453 4 25.443117372432404 18.482186573751108 33.6971545517434 22.377541502073086 5 36.64000155481702 17.470856898528755 31.8183585019338 14.070783044720425 6 30.299729850543216 18.036188366081667 37.440712883602515 14.223368899772609 7 30.01142790507842 26.310594134452803 28.437933648183776 15.240044312284997 8 25.38903465298428 33.8432744446388 26.27135443997435 14.496336462402581 9 36.29180028375411 23.301148621071658 24.49369327347288 15.913357821701357 10-11 26.298175033525744 30.866402347773697 28.072746001204983 14.762676617495579 12-13 22.48451985306979 29.164237265076864 27.481176996482226 20.870065885371115 14-15 21.397537558548578 29.985588789769302 28.089061862282083 20.527811789400037 16-17 24.588809204516743 26.627689347559908 28.76425086972577 20.01925057819758 18-19 19.15520960876917 27.00232250791985 33.220609099566595 20.621858783744386 20-21 23.547030361795336 27.03653636421677 32.959807871205136 16.456625402782755 22-23 18.990447966801497 35.83532285436862 28.674601946789412 16.499627232040474 24-25 19.002021262122714 27.066847414144945 32.97308223038501 20.958049093347327 26-27 19.095018754980273 27.17887198025382 28.27105319417721 25.455056070588693 28-29 22.920918119448817 31.59764250869726 28.287738324295958 17.193701047557965 30-31 18.151614094416264 27.867753095057623 32.32089479719355 21.659738013332554 32-33 17.932977668940588 32.23881989388374 28.441179328707754 21.387023108467922 34-35 22.576244339494295 32.2183448972849 28.64763959341535 16.557771169805456 36-37 18.09187226271308 32.263206733727046 33.17571587927421 16.469205124285665 38-39 18.508561377594653 27.88997512244422 32.51331337946047 21.08815012050066 40-41 22.71714638511937 27.76333980369668 32.259902192362794 17.259611618821157 42-43 18.397706046990198 32.08529459467837 32.57619097642755 16.940808381903878 44-45 17.98645737269532 27.94093952948699 32.81347693545582 21.259126162361873 46-47 22.435213518699896 27.852319872674265 28.38528939968361 21.327177208942228 48-49 18.538611957056858 32.28609039734475 32.02320097319232 17.152096672406074 50-51 22.885619035643145 27.804842638447518 31.674419119914997 17.63511920599434 52-53 18.284956644815995 27.646103670483303 27.71655699862796 26.35238268607274 54-55 22.743146294519477 27.713213351427207 31.809393410407576 17.73424694364574 56-57 18.492141694931135 27.5265655881989 31.856757193648967 22.124535523220995 58-59 18.511391822373746 27.600978151195488 32.26257288677557 21.625057139655198 60-61 22.550151675202475 28.033321788514403 31.990126215037108 17.426400321246007 62-63 18.20441112973866 28.146785374601425 31.957692586902432 21.691110908757487 64-65 22.601565697290813 27.9204969972709 31.97038371158562 17.507553593852666 66-67 18.61385320561391 36.03261384640134 27.5138892196521 17.839643728332646 68-69 18.443508729228903 36.27540911835998 27.542583720337298 17.73849843207382 70-71 18.09696494387108 36.789678779491936 27.603401368743942 17.509954907893043 72-73 17.96184780629698 36.6427613180697 27.549257697078662 17.84613317855465 74-75 18.485204700727493 35.569788125847104 27.53406429232345 18.410942881101956 76-77 18.94286255523966 34.64599623446469 27.88109570448858 18.53004550580707 78-79 19.001857529535283 33.9678037932555 28.337768124762075 18.692570552447147 80-81 18.5776460297405 34.02909903780736 28.16960831956458 19.22364661288755 82-83 18.149787416168383 33.63392159418097 28.33254760553218 19.883743384118464 84-85 18.23682515635692 33.04215127763525 28.918923568136222 19.802099997871604 86-87 18.146895262995656 32.873740251233805 29.492369596749164 19.486994889021375 88-89 18.46732209250961 33.13622286357692 29.008876968913842 19.387578074999624 90-91 18.792451275041156 32.66202474570179 28.78435187234416 19.761172106912895 92-93 18.45024775132796 33.05990998020031 28.717210562621315 19.77263170585041 94-95 18.21411997075691 33.23290055648017 28.581298871161902 19.97168060160102 96-97 18.66385442036841 33.397777403822644 28.247190305605272 19.691177870203674 98-99 18.963345412776526 32.6183558930982 28.08017538426064 20.338123309864645 100-101 18.839732285244978 32.63737179227327 27.876247494512207 20.64664842796954 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 377.0 1 265.0 2 234.0 3 558.0 4 674.5 5 437.5 6 340.5 7 360.0 8 340.5 9 335.0 10 353.0 11 350.5 12 339.5 13 345.0 14 397.5 15 480.5 16 571.0 17 721.0 18 1051.5 19 1594.0 20 2478.5 21 3795.0 22 5805.5 23 8955.5 24 13206.0 25 18806.0 26 26361.5 27 36168.5 28 47180.5 29 58655.5 30 71046.5 31 84448.0 32 100155.5 33 121163.0 34 148432.0 35 186869.0 36 243961.0 37 314291.0 38 377521.5 39 398400.5 40 396297.5 41 369444.0 42 319562.5 43 295182.0 44 268327.5 45 238997.5 46 231998.5 47 203901.5 48 144312.0 49 97917.0 50 71948.0 51 55667.5 52 43709.5 53 32556.0 54 24488.5 55 18708.0 56 14046.5 57 10399.0 58 7893.5 59 6193.5 60 4643.0 61 3306.0 62 2483.0 63 1847.5 64 1308.0 65 875.0 66 542.5 67 351.5 68 234.5 69 150.5 70 106.5 71 73.0 72 53.0 73 41.5 74 23.0 75 14.5 76 13.5 77 10.0 78 9.0 79 10.0 80 5.5 81 1.5 82 2.0 83 3.5 84 2.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1650243911919616 2 0.0 3 0.0016519930810642725 4 0.010825413484150583 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.9152819077604805E-5 22-23 1.554817017472256E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.093852642217169E-4 38-39 0.0019435212718403204 40-41 0.00588886945367617 42-43 0.0023419431325675862 44-45 0.0012632888266962082 46-47 0.001389617709365829 48-49 0.0033234213748469478 50-51 9.134549977649505E-4 52-53 0.014761044059627233 54-55 0.026927487221347638 56-57 0.028657221153285523 58-59 0.02148562766019474 60-61 0.03067848327599946 62-63 0.005665364507414534 64-65 0.010951742366820205 66-67 0.028618350727848713 68-69 0.027277321050278896 70-71 0.031212951625755544 72-73 0.031319845295706764 74-75 0.03908421277670884 76-77 0.01965871766466484 78-79 0.010951742366820205 80-81 0.015548170174722563 82-83 0.007550580141099644 84-85 0.011282140983033059 86-87 0.03429343284162245 88-89 0.015693934270110586 90-91 0.021252405107573903 92-93 0.04941402833654015 94-95 0.056138611937107646 96-97 0.04043496006063786 98-99 0.035974578741764324 100-101 0.035770509008221096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5145300.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.720905633627076 #Duplication Level Percentage of deduplicated Percentage of total 1 93.03609724545171 47.18875108907509 2 3.385684815770134 3.4345000009176214 3 1.0220712434264478 1.5552113726603027 4 0.6259533749959 1.269956882568697 5 0.3112592962984964 0.7893676697572605 6 0.2259156645085443 0.6875188260417618 7 0.14464912227480928 0.5135714136621311 8 0.15202885018455345 0.6168832771039651 9 0.08792403644434449 0.4013628079879067 >10 0.7807909542483519 7.9556647170388795 >50 0.09925826281654344 3.6160175572920705 >100 0.10145437771999105 10.802112865761478 >500 0.018409433505354903 6.451059734113711 >1k 0.008190626841008034 6.081496076224031 >5k 2.736090097933043E-4 0.8922548953937468 >10k+ 3.9086503927252105E-5 7.744270814401339 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 390631 7.5919965794025615 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8333 0.1619536275824539 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7405 0.14391775017977573 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6524 0.1267953277748625 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6280 0.12205313587157211 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5910 0.11486210716576294 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5309 0.10318154432200259 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTCGTATCTCGTAT 5245 0.10193769070802479 TruSeq Adapter, Index 11 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001341029677569821 0.0 0.0 2 0.0015353818047538531 0.0 0.0 3 0.0016519930810642723 0.0 1.9435212718403204E-5 4 0.0016908635065010786 0.0 1.9435212718403204E-5 5 0.00200182690999553 0.0 1.9435212718403204E-5 6 0.0021573086117427556 0.0 3.887042543680641E-5 7 0.002409966377081997 0.0 3.887042543680641E-5 8 0.0028958466950420774 0.0 7.774085087361282E-5 9 0.003187374885818125 0.0 7.774085087361282E-5 10-11 0.0059277398791129764 0.0 8.745845723281441E-5 12-13 0.00776436748100208 0.0 1.3604648902882242E-4 14-15 0.009669018327405594 0.0 1.5548170174722563E-4 16-17 0.011010048004975414 0.0 1.6519930810642724E-4 18-19 0.01238023050162284 0.0 1.8463452082483043E-4 20-21 0.013750412998270265 0.0 1.9435212718403204E-4 22-23 0.015276077196664918 0.0 1.9435212718403204E-4 24-25 0.016966940703165997 0.0 1.9435212718403204E-4 26-27 0.01875498027325909 0.0 1.9435212718403204E-4 28-29 0.021223252288496298 0.0 1.9435212718403204E-4 30-31 0.03895788389403922 0.0 2.1378733990243522E-4 32-33 0.04232989330068218 0.0 2.2350494626163683E-4 34-35 0.044506637125143335 0.0 2.3322255262083844E-4 36-37 0.046693098555963694 0.0 2.3322255262083844E-4 38-39 0.04920995860299691 0.0 2.3322255262083844E-4 40-41 0.05210580529803899 0.0 2.526577653392416E-4 42-43 0.0558276485336132 0.0 2.526577653392416E-4 44-45 0.05962723262006103 0.0 2.526577653392416E-4 46-47 0.06342681670650885 0.0 2.526577653392416E-4 48-49 0.06788719802538239 0.0 2.526577653392416E-4 50-51 0.07287233008765281 0.0 2.526577653392416E-4 52-53 0.07876119954132899 0.0 2.526577653392416E-4 54-55 0.08474724505859717 1.9435212718403204E-5 2.623753716984432E-4 56-57 0.09144267584008707 1.9435212718403204E-5 2.7209297805764484E-4 58-59 0.0994597010864284 1.9435212718403204E-5 2.7209297805764484E-4 60-61 0.10880803840398034 1.9435212718403204E-5 2.9152819077604805E-4 62-63 0.11894350183662761 1.9435212718403204E-5 3.1096340349445127E-4 64-65 0.1303131012768935 1.9435212718403204E-5 3.303986162128545E-4 66-67 0.14287796629934113 1.9435212718403204E-5 3.6926904164966086E-4 68-69 0.1572891765300371 1.9435212718403204E-5 3.8870425436806407E-4 70-71 0.17277904106660447 1.9435212718403204E-5 3.8870425436806407E-4 72-73 0.19075661283112744 1.9435212718403204E-5 4.081394670864673E-4 74-75 0.21163003129069247 1.9435212718403204E-5 4.081394670864673E-4 76-77 0.23504946261636833 1.9435212718403204E-5 4.081394670864673E-4 78-79 0.26063592016014614 1.9435212718403204E-5 4.372922861640721E-4 80-81 0.2904495364701767 1.9435212718403204E-5 4.4700989252327366E-4 82-83 0.32367403261228694 1.9435212718403204E-5 4.5672749888247524E-4 84-85 0.36099935863798027 1.9435212718403204E-5 4.664451052416769E-4 86-87 0.4021534215691991 1.9435212718403204E-5 4.858803179600801E-4 88-89 0.4496822342720541 1.9435212718403204E-5 4.955979243192817E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 47845 0.0 88.437836 3 GATCGGA 48015 0.0 88.32959 1 ATCGGAA 48015 0.0 87.996124 2 CGGAAGA 47940 0.0 87.77628 4 GAAGAGC 49010 0.0 85.542336 6 GGAAGAG 51455 0.0 81.86524 5 AGAGCAC 66410 0.0 63.108047 8 GAGCACA 66735 0.0 62.878994 9 AAGAGCA 68170 0.0 61.395134 7 TCGGACG 205 0.0 60.23726 3 ATGCCGT 44385 0.0 46.367252 48-49 GCCGTCT 44565 0.0 46.353218 50-51 TATGCCG 44645 0.0 46.32598 48-49 TCGTATG 45100 0.0 46.27092 44-45 TGCCGTC 44555 0.0 46.262337 50-51 CGTATGC 44870 0.0 46.247982 46-47 TCTCGTA 44700 0.0 46.243824 42-43 CTCGTAT 44700 0.0 46.217487 44-45 TATCTCG 44395 0.0 46.194477 40-41 CGTCTTC 44780 0.0 46.168694 52-53 >>END_MODULE