FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145300
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3906317.5919965794025615TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC83330.1619536275824539No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC74050.14391775017977573No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG65240.1267953277748625No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA62800.12205313587157211No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG59100.11486210716576294No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA53090.10318154432200259No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTCGTATCTCGTAT52450.10193769070802479TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG478450.088.4378363
GATCGGA480150.088.329591
ATCGGAA480150.087.9961242
CGGAAGA479400.087.776284
GAAGAGC490100.085.5423366
GGAAGAG514550.081.865245
AGAGCAC664100.063.1080478
GAGCACA667350.062.8789949
AAGAGCA681700.061.3951347
TCGGACG2050.060.237263
ATGCCGT443850.046.36725248-49
GCCGTCT445650.046.35321850-51
TATGCCG446450.046.3259848-49
TCGTATG451000.046.2709244-45
TGCCGTC445550.046.26233750-51
CGTATGC448700.046.24798246-47
TCTCGTA447000.046.24382442-43
CTCGTAT447000.046.21748744-45
TATCTCG443950.046.19447740-41
CGTCTTC447800.046.16869452-53
Produced by FastQC (version 0.11.2)