FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6454864
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1638472.5383493749829587TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC64790.10037391957444805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA269650.065.7957844
TCGGAAG272100.065.605793
GAAGAGC273250.064.927446
GATCGGA279650.064.002641
ATCGGAA282050.063.2745482
GGAAGAG300450.059.2233625
TTGTGTT593800.046.2343331
GCCGTCT198350.044.14210550-51
ATGCCGT198000.044.0696848-49
TCGTATG202300.043.8395944-45
CGTATGC200850.043.82879346-47
TGCCGTC199100.043.80879650-51
TATGCCG200400.043.7196748-49
CGTCTTC201200.043.4711252-53
CTCGTAT200650.043.4072144-45
TCTCGTA201000.043.34327342-43
CATCTCG201400.043.04680340-41
ATCTCGT201850.042.854942-43
GTATGCC207750.042.47598346-47
CTGCTTG209700.042.20764558-59
Produced by FastQC (version 0.11.2)