##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_52260024-1.34100000035820.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6454864 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67134690986518 33.0 33.0 33.0 33.0 33.0 2 32.65660639790397 33.0 33.0 33.0 33.0 33.0 3 32.722687883121935 33.0 33.0 33.0 33.0 33.0 4 36.674411265675 37.0 37.0 37.0 37.0 37.0 5 36.681850771759095 37.0 37.0 37.0 37.0 37.0 6 36.56097494850395 37.0 37.0 37.0 37.0 37.0 7 36.49449004657573 37.0 37.0 37.0 37.0 37.0 8 36.4357882675762 37.0 37.0 37.0 37.0 37.0 9 36.45752226538003 37.0 37.0 37.0 37.0 37.0 10-11 36.33242327026565 37.0 37.0 37.0 37.0 37.0 12-13 36.36657410597652 37.0 37.0 37.0 37.0 37.0 14-15 38.27050771325314 40.0 37.0 40.0 37.0 40.0 16-17 38.23527683929514 40.0 37.0 40.0 37.0 40.0 18-19 38.15782942909409 40.0 37.0 40.0 37.0 40.0 20-21 38.15456065379534 40.0 37.0 40.0 37.0 40.0 22-23 38.052252921207945 40.0 37.0 40.0 37.0 40.0 24-25 38.07891088022923 40.0 37.0 40.0 37.0 40.0 26-27 38.01161744073926 40.0 37.0 40.0 37.0 40.0 28-29 37.88067014270169 40.0 37.0 40.0 33.0 40.0 30-31 37.78229595542214 40.0 37.0 40.0 33.0 40.0 32-33 37.68131714316522 40.0 37.0 40.0 33.0 40.0 34-35 37.58408232923266 40.0 37.0 40.0 33.0 40.0 36-37 37.46262314744354 40.0 37.0 40.0 33.0 40.0 38-39 37.4028331193345 40.0 37.0 40.0 33.0 40.0 40-41 37.28495743674847 40.0 37.0 40.0 33.0 40.0 42-43 37.178489508067095 40.0 37.0 40.0 33.0 40.0 44-45 37.075180669956794 37.0 37.0 40.0 33.0 40.0 46-47 37.035625305196206 37.0 37.0 40.0 33.0 40.0 48-49 36.83741028470933 37.0 37.0 40.0 33.0 40.0 50-51 36.70192400645467 37.0 37.0 40.0 33.0 40.0 52-53 36.536062727270476 37.0 37.0 40.0 33.0 40.0 54-55 36.17828973623612 37.0 37.0 40.0 33.0 40.0 56-57 36.05494151387233 37.0 37.0 40.0 33.0 40.0 58-59 35.902258901194514 37.0 37.0 40.0 33.0 40.0 60-61 35.56743968579353 37.0 33.0 40.0 27.0 40.0 62-63 35.323840362864345 37.0 33.0 38.5 27.0 40.0 64-65 35.0838734170077 37.0 33.0 37.0 27.0 40.0 66-67 34.559629993753546 37.0 33.0 37.0 27.0 40.0 68-69 34.319531131872026 37.0 33.0 37.0 27.0 40.0 70-71 34.01431532871955 37.0 33.0 37.0 27.0 38.5 72-73 33.5267802698864 37.0 33.0 37.0 27.0 37.0 74-75 33.013010033983676 37.0 33.0 37.0 27.0 37.0 76-77 30.353360272191637 33.0 30.0 35.0 22.0 37.0 78-79 32.74725764942531 35.0 33.0 37.0 27.0 37.0 80-81 33.13684641535437 37.0 33.0 37.0 27.0 37.0 82-83 33.040392717801645 37.0 33.0 37.0 27.0 37.0 84-85 32.93407808437172 37.0 33.0 37.0 27.0 37.0 86-87 32.81526380726224 37.0 33.0 37.0 27.0 37.0 88-89 32.6715232110235 37.0 33.0 37.0 27.0 37.0 90-91 32.57845231131128 35.0 33.0 37.0 24.5 37.0 92-93 32.4107399474257 33.0 33.0 37.0 22.0 37.0 94-95 32.28966442980054 33.0 33.0 37.0 22.0 37.0 96-97 32.129625039350174 33.0 33.0 37.0 22.0 37.0 98-99 31.99839446656041 33.0 33.0 37.0 22.0 37.0 100-101 30.36050658851991 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15598466045759096 2306 2 0.18020330985370947 2306 3 0.13552896929066804 2306 4 0.16666889486678826 2306 5 0.15493984807264383 2306 6 0.1803507313342365 2306 7 0.21271458425286482 2306 8 0.18542990803153714 2306 9 0.19633023647882197 2306 10-11 0.24639228911385658 2306 12-13 0.2477248607934186 2306 14-15 0.41203748608310065 2306 16-17 0.4409341849675741 2306 18-19 0.5391017856663254 2306 20-21 0.5109785244939573 2306 22-23 0.4867131732992078 2306 24-25 0.4535414568523848 2306 26-27 0.48812982086830914 2306 28-29 0.5689220192923301 2306 30-31 0.5201997427714673 2306 32-33 0.52131831015204 2306 34-35 0.49422506212734874 2306 36-37 0.4766759697766716 2306 38-39 0.5455082783107983 2306 40-41 0.6077822287516952 2306 42-43 0.567460280385724 2306 44-45 0.5194403967667114 2306 46-47 0.4994853974303126 2306 48-49 0.4930822228408971 2306 50-51 0.5019182174093686 2306 52-53 0.47661133255334676 2306 54-55 0.6362725243058023 2306 56-57 0.6366235999095409 2306 58-59 0.5420692153568893 2306 60-61 0.5089364524610858 2306 62-63 0.5335762518807172 2306 64-65 0.48833707369361434 2306 66-67 0.6060035853851886 2306 68-69 0.489332937974126 2306 70-71 0.34364418556858567 2306 72-73 0.3860865203345156 2306 74-75 0.30435200456678757 2306 76-77 0.015296768304491337 2306 78-79 0.32768902646990483 2306 80-81 0.44460766396725404 2306 82-83 0.4860776859141538 2306 84-85 0.4702377860891289 2306 86-87 0.5873703298330142 2306 88-89 0.48363334033422944 2306 90-91 0.3623667021482433 2306 92-93 0.2895210459478079 2306 94-95 0.28237104384341194 2306 96-97 0.5449176372163933 2306 98-99 0.5476443624901037 2306 100-101 0.3001813309873924 2307 1 0.11572638313587902 2307 2 0.19314351968878896 2307 3 0.14257667871057578 2307 4 0.16133477513693606 2307 5 0.13542932027385035 2307 6 0.15728157240649665 2307 7 0.16002508325153286 2307 8 0.1947204406042431 2307 9 0.1909385787966258 2307 10-11 0.24649993358930544 2307 12-13 0.21508126199518784 2307 14-15 0.375385276438827 2307 16-17 0.36527306811007776 2307 18-19 0.469375710819115 2307 20-21 0.4787305046806054 2307 22-23 0.44440019478413717 2307 24-25 0.3926072745739333 2307 26-27 0.3507787631756756 2307 28-29 0.4232294492442108 2307 30-31 0.46662029474065747 2307 32-33 0.43402115479636905 2307 34-35 0.5251893136596024 2307 36-37 0.4564431916022542 2307 38-39 0.44386490450243343 2307 40-41 0.5778800708604237 2307 42-43 0.4926637902987139 2307 44-45 0.42325803323044653 2307 46-47 0.3230024134453586 2307 48-49 0.47678429746454754 2307 50-51 0.4784690765982873 2307 52-53 0.49201047773428286 2307 54-55 0.5401472982257118 2307 56-57 0.5854339348827722 2307 58-59 0.4158983202514719 2307 60-61 0.3286218546954274 2307 62-63 0.3258544094772162 2307 64-65 0.40826849628633255 2307 66-67 0.43775931973410565 2307 68-69 0.38028098543061617 2307 70-71 0.35557943921883606 2307 72-73 0.22304320943074885 2307 74-75 0.29263953804604625 2307 76-77 0.0011298608951157973 2307 78-79 0.2953788852478354 2307 80-81 0.4260550340511884 2307 82-83 0.3325395290403321 2307 84-85 0.3866910550369198 2307 86-87 0.4239835711981783 2307 88-89 0.45603732849307477 2307 90-91 0.4344497193120844 2307 92-93 0.32265020187450943 2307 94-95 0.42786567977546497 2307 96-97 0.5521418542062975 2307 98-99 0.41245771969656886 2307 100-101 0.27760498261907784 2201 1 0.14018251310316998 2201 2 0.18222248802405971 2201 3 0.11705218179509558 2201 4 0.10850758049710407 2201 5 0.13107238446075797 2201 6 0.09816273860649716 2201 7 0.16517875925902814 2201 8 0.19330634532170876 2201 9 0.160969646921707 2201 10-11 0.20710119025726215 2201 12-13 0.21656234510657413 2201 14-15 0.30653257757627017 2201 16-17 0.25954392515592417 2201 18-19 0.3900339628659708 2201 20-21 0.3345299552144283 2201 22-23 0.4040001908952888 2201 24-25 0.2841306821368903 2201 26-27 0.2619436542499116 2201 28-29 0.24440834492435926 2201 30-31 0.42246334071635516 2201 32-33 0.4646457374562658 2201 34-35 0.46632971682911517 2201 36-37 0.42474229830473575 2201 38-39 0.39444164006815896 2201 40-41 0.5340048806861333 2201 42-43 0.42810246217074877 2201 44-45 0.5027068431472088 2201 46-47 0.398177222038008 2201 48-49 0.3717065370443322 2201 50-51 0.4106229736063369 2201 52-53 0.4882899855764862 2201 54-55 0.6642044889956651 2201 56-57 0.5621084171913466 2201 58-59 0.5058290062258024 2201 60-61 0.47533610761870193 2201 62-63 0.4494557273234321 2201 64-65 0.362844345952837 2201 66-67 0.5191850412582326 2201 68-69 0.4846229467962999 2201 70-71 0.40606407541209677 2201 72-73 0.4208868415795948 2201 74-75 0.42313894238790084 2201 76-77 0.18539719426259538 2201 78-79 0.4476980631637062 2201 80-81 0.4329259104464356 2201 82-83 0.5398536395937228 2201 84-85 0.524259098072271 2201 86-87 0.5790131478066414 2201 88-89 0.6598644557893962 2201 90-91 0.595822865468385 2201 92-93 0.43098309652648226 2201 94-95 0.4402669053326207 2201 96-97 0.5120416356718422 2201 98-99 0.4822249506260263 2201 100-101 0.49124221068406015 1101 1 0.10491142019767352 1101 2 0.141841876998285 1101 3 0.07744335349675424 1101 4 0.11533653310562642 1101 5 0.11565351320854944 1101 6 0.11783226412279646 1101 7 0.15627266037981258 1101 8 0.13870676205949195 1101 9 0.2088368526450921 1101 10-11 0.19640534127847786 1101 12-13 0.14329941627912746 1101 14-15 0.2248785339397017 1101 16-17 0.2794572885000619 1101 18-19 0.3340400681456046 1101 20-21 0.4005754087901252 1101 22-23 0.3552770548368045 1101 24-25 0.2995614474836543 1101 26-27 0.297887074628818 1101 28-29 0.2658486148794168 1101 30-31 0.34722965464933964 1101 32-33 0.24757396041808732 1101 34-35 0.29291682284834764 1101 36-37 0.34042027947330666 1101 38-39 0.40371746927677776 1101 40-41 0.4789859105831553 1101 42-43 0.4216418175783829 1101 44-45 0.41423506539787525 1101 46-47 0.407385420251579 1101 48-49 0.3697514785872471 1101 50-51 0.2764742636444524 1101 52-53 0.2627621651293097 1101 54-55 0.47391632833115693 1101 56-57 0.38807985714127824 1101 58-59 0.4009161406913009 1101 60-61 0.4525629523589956 1101 62-63 0.3866961195974312 1101 64-65 0.260422857644258 1101 66-67 0.3984037823687743 1101 68-69 0.43580917382594464 1101 70-71 0.3452410538498256 1101 72-73 0.14310984607410404 1101 74-75 0.2708572674718184 1101 76-77 0.08492756420627146 1101 78-79 0.4075795844699641 1101 80-81 0.500985928509813 1101 82-83 0.5563373078818259 1101 84-85 0.5104927236909731 1101 86-87 0.461430173607404 1101 88-89 0.3384221234801217 1101 90-91 0.32940448135846623 1101 92-93 0.32377515077351404 1101 94-95 0.3648474118183671 1101 96-97 0.4198053325484423 1101 98-99 0.3851731393101048 1101 100-101 0.3728242141545053 2304 1 0.10457229860561768 2304 2 0.1456703982247589 2304 3 0.07367526786999434 2304 4 0.07380044552228071 2304 5 0.08133188511597211 2304 6 0.041100613632359284 2304 7 0.0995082819847326 2304 8 0.10844165637764291 2304 9 0.13851874513537155 2304 10-11 0.12123697117317533 2304 12-13 0.09789575603146261 2304 14-15 0.14058578814251632 2304 16-17 0.12519791422413107 2304 18-19 0.2890329240012264 2304 20-21 0.2659988280677297 2304 22-23 0.20637179841994424 2304 24-25 0.1664172837990776 2304 26-27 0.15167635133246904 2304 28-29 0.24257308904464736 2304 30-31 0.30747157404279335 2304 32-33 0.24351518534922434 2304 34-35 0.26013931145633507 2304 36-37 0.21460280592594927 2304 38-39 0.24850700664516268 2304 40-41 0.34386299590097735 2304 42-43 0.2539277346033302 2304 44-45 0.22055430245475094 2304 46-47 0.2148466906220463 2304 48-49 0.19145326526695783 2304 50-51 0.12319043515540784 2304 52-53 0.09903576976849848 2304 54-55 0.2549359618558569 2304 56-57 0.2955307652843189 2304 58-59 0.3019706582362929 2304 60-61 0.2673346937280954 2304 62-63 0.13910781539847505 2304 64-65 0.11609995840886711 2304 66-67 0.26773612667791724 2304 68-69 0.2066862140905954 2304 70-71 0.10568883401612794 2304 72-73 -0.006271927990717074 2304 74-75 0.12056204053259734 2304 76-77 -0.11272412013839883 2304 78-79 0.0964109054286908 2304 80-81 0.22897380599095385 2304 82-83 0.09900588716252656 2304 84-85 0.1597840696220345 2304 86-87 0.1698496925683557 2304 88-89 0.11954594244364358 2304 90-91 0.05799859741541269 2304 92-93 0.01943801152062008 2304 94-95 0.134816421678579 2304 96-97 0.19725268470971002 2304 98-99 0.16789669953974595 2304 100-101 0.23362099542783454 2202 1 0.11832581249521468 2202 2 0.1471156288990727 2202 3 0.09875920036956387 2202 4 0.06371020255521387 2202 5 0.12093428815700236 2202 6 0.08529838255860511 2202 7 0.0893260309012689 2202 8 0.08100577372795925 2202 9 0.00540907943074842 2202 10-11 0.16876308975746213 2202 12-13 0.15498547367320725 2202 14-15 0.16125230548645675 2202 16-17 0.15424578493362162 2202 18-19 0.1850746527880247 2202 20-21 0.20358119584327738 2202 22-23 0.1747965174963113 2202 24-25 0.14366332498870804 2202 26-27 -0.02856910170846305 2202 28-29 0.12590462909876976 2202 30-31 0.19934829465317705 2202 32-33 0.20644306411323043 2202 34-35 0.18578459309258477 2202 36-37 0.17531167747745968 2202 38-39 0.24018009629880765 2202 40-41 0.32263845226123067 2202 42-43 0.23923758394912653 2202 44-45 0.24381328033716443 2202 46-47 0.250716758049343 2202 48-49 0.2076510304425625 2202 50-51 0.21449020627203197 2202 52-53 0.25615811805941746 2202 54-55 0.33965952610729033 2202 56-57 0.36130464262120654 2202 58-59 0.2785782392831351 2202 60-61 0.2515741722190583 2202 62-63 0.14947449294202642 2202 64-65 0.17197352800498322 2202 66-67 0.2608964073952649 2202 68-69 0.1410498214932545 2202 70-71 0.18594103822586305 2202 72-73 0.1347927477375137 2202 74-75 0.12709907069345405 2202 76-77 -0.03635559610329864 2202 78-79 0.2234920469763253 2202 80-81 0.3068818543687044 2202 82-83 0.2730441327681632 2202 84-85 0.24571466901330297 2202 86-87 0.2775787435509258 2202 88-89 0.17391594118717535 2202 90-91 0.06305617222680127 2202 92-93 0.017860043022324135 2202 94-95 0.14126853479440626 2202 96-97 0.15753928589003863 2202 98-99 0.16509619517117358 2202 100-101 0.1777846979094555 1102 1 0.12431813419602378 1102 2 0.15745555654801535 1102 3 0.10402426345069671 1102 4 0.09102366252754734 1102 5 0.01855636288907192 1102 6 0.048836324778370965 1102 7 0.027377372335052996 1102 8 0.04796367262125756 1102 9 0.050440262452347895 1102 10-11 0.17319223283836038 1102 12-13 0.09107943075245117 1102 14-15 0.10607229747083124 1102 16-17 0.10681706499099164 1102 18-19 0.19018724471867188 1102 20-21 0.16872851009889445 1102 22-23 0.13338214743148313 1102 24-25 0.1375651365268027 1102 26-27 0.04336029371013694 1102 28-29 0.15798349062998085 1102 30-31 0.21048509982447428 1102 32-33 0.167518699996009 1102 34-35 0.11105901650505956 1102 36-37 0.07414628828067293 1102 38-39 0.1234310869914097 1102 40-41 0.26199496318211146 1102 42-43 0.12182329081853283 1102 44-45 0.07574716687364713 1102 46-47 0.005344154723225358 1102 48-49 0.1031599618819925 1102 50-51 0.08181063306631131 1102 52-53 0.06611174710295131 1102 54-55 0.12889203811840844 1102 56-57 0.25716651806074253 1102 58-59 0.1818290886008711 1102 60-61 0.2177337962802639 1102 62-63 0.10515725300027157 1102 64-65 0.11643786356447805 1102 66-67 0.1527721691345647 1102 68-69 0.11331971663584284 1102 70-71 0.10108250021952614 1102 72-73 0.01549233304886144 1102 74-75 0.05323259300881489 1102 76-77 -0.23408108819468865 1102 78-79 0.16544558133156784 1102 80-81 0.19671721511627283 1102 82-83 0.055915926476593825 1102 84-85 0.1499406761366231 1102 86-87 0.16034010047348346 1102 88-89 0.07435630745987254 1102 90-91 0.04794944570924997 1102 92-93 -0.03013672442489934 1102 94-95 -0.041193321115670756 1102 96-97 0.08814485691463148 1102 98-99 0.20284624374823323 1102 100-101 0.1551850212304835 2305 1 0.0982537553325713 2305 2 0.11449242488944122 2305 3 0.06819331458145683 2305 4 0.05753352775884224 2305 5 0.022469807676060327 2305 6 -0.04340882608466501 2305 7 0.041201544940754786 2305 8 0.03332848729216664 2305 9 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-1.6056712245324007 1213 98-99 -1.688080328827816 1213 100-101 -1.5942657873698423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 11.0 8 114.0 9 316.0 10 201.0 11 95.0 12 83.0 13 167.0 14 452.0 15 1026.0 16 2063.0 17 3517.0 18 5414.0 19 7505.0 20 10497.0 21 14013.0 22 18547.0 23 24445.0 24 31857.0 25 41468.0 26 54598.0 27 75956.0 28 113184.0 29 151315.0 30 141464.0 31 146347.0 32 193959.0 33 277300.0 34 429983.0 35 760762.0 36 1483613.0 37 2061351.0 38 402870.0 39 371.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.37821803410277 25.863411713938177 21.87561049371883 15.88275975824022 2 29.609810524280604 25.061395561548622 31.043148236740542 14.285645677430228 3 35.699269064284536 19.464037281129837 27.264165558953575 17.572528095632048 4 28.805658236865927 19.279191145687914 31.75156945142099 20.163581166025168 5 33.748766821423345 17.959712241807107 30.75685250688473 17.53466842988481 6 28.73845211920809 19.044646021976604 34.83696945435256 17.379932404462743 7 31.867472343336743 21.812481254446258 27.494413515141453 18.825632887075542 8 28.490638997196534 27.76255239459731 25.21659635276591 18.53021225544024 9 30.994843578423957 23.427883221087228 25.936487585176078 19.64078561531273 10-11 24.976010338870037 28.50018993428831 29.075035508106755 17.448764218734894 12-13 23.776490393638845 27.02287197862511 28.565902284378282 20.634735343357764 14-15 23.41256144203813 27.42960967109454 28.633097459528194 20.524731427339134 16-17 24.331643548183198 26.380036821844737 29.270516001576485 20.017803628395576 18-19 22.02776851688897 26.434546103527513 30.9139743300556 20.62371104952792 20-21 23.377190451384518 26.743234267190047 30.486094785132913 19.393480496292522 22-23 22.0026192681433 29.778191041117648 28.69393765763809 19.52525203310097 24-25 21.875007746096585 26.23676501937144 30.660080522223243 21.228146712308735 26-27 21.71058909994076 26.3819655998949 29.21238154669099 22.69506375347335 28-29 22.801611312027642 28.441149186102137 28.656839245567372 20.100400256302844 30-31 20.902570526660206 27.557613917194846 30.107350054160708 21.43246550198424 32-33 20.721482280649138 28.934846652075088 28.97961909034799 21.364051976927787 34-35 22.511008752469454 29.105245284796084 29.108707789970477 19.27503817276398 36-37 22.20413150525085 29.41099236856458 29.455377842384163 18.92949828380041 38-39 21.073243485825905 27.651986292578425 31.83795689113671 19.436813330458964 40-41 21.079119068641706 27.32860876472445 28.6729913624429 22.919280804190944 42-43 21.346492403262328 28.90835632235461 30.135374756873333 19.60977651750973 44-45 20.90967328139258 27.580649409198436 30.421469947124542 21.088207362284443 46-47 22.45998393129311 27.47379384772406 29.01625492541808 21.049967295564745 48-49 21.117922545679725 29.242467739244262 29.98369862188131 19.655911093194707 50-51 22.416467261400573 27.828524097381806 29.411705470617726 20.343303170599892 52-53 20.827415724626743 27.526704000153707 28.35492388945478 23.290956385764765 54-55 22.513680102816075 27.64596080644635 29.697819326952036 20.142539763785535 56-57 21.231240903897035 27.325226006414567 29.408569186635702 22.034963903052695 58-59 21.202102429927397 27.442753798916396 29.57250229914535 21.78264147201086 60-61 22.320836984508276 27.673409727187202 29.479764038469003 20.52598924983552 62-63 20.852477552151083 27.743497730891054 29.4867810382684 21.917243678689463 64-65 22.663887887380422 27.6748862321671 29.386574814696832 20.27465106575565 66-67 21.74175679638864 30.369500543742085 27.680551622180026 20.20819103768925 68-69 21.792315561568557 30.27016120815344 27.727349892984787 20.210173337293217 70-71 21.470101444019825 30.831512424025465 27.786603376508257 19.911782755446456 72-73 21.423653308463564 31.12479299728769 27.46105807481821 19.990495619430533 74-75 21.97669388848843 30.38159731903311 27.27833133585409 20.363377456624377 76-77 22.075479957403406 29.498335610497605 27.785275353515708 20.64090907858328 78-79 21.827891398567346 29.2022179772485 28.473193063274294 20.496697560909855 80-81 21.373312586019424 29.496513513838064 28.311584678751707 20.818589221390805 82-83 20.55092756671334 29.54551458213502 28.5624933427068 21.341064508444838 84-85 20.6952570377019 29.485875015552054 28.529743198580874 21.289124748165168 86-87 20.506214392973792 29.627881852726517 29.07219469731892 20.793709056980774 88-89 20.648438608230464 29.880397589559642 28.908139439123193 20.563024363086697 90-91 21.015932612579665 29.381205033602285 28.40174139953482 21.201120954283226 92-93 20.63563326061562 29.88223729379437 28.07886434817827 21.403265097411744 94-95 20.407641451985313 30.062080616368235 28.036219880352647 21.4940580512938 96-97 20.73948902842961 30.52166637464734 27.67273046543748 21.06611413148557 98-99 20.976501671278804 30.022391168506562 27.621245201892464 21.379861958322174 100-101 21.08543092055431 29.853619943425493 27.26086304605813 21.800086089962072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21.0 1 12.5 2 20.0 3 56.5 4 68.5 5 49.0 6 49.0 7 53.0 8 48.0 9 53.0 10 48.5 11 44.5 12 60.0 13 67.5 14 69.0 15 82.5 16 100.0 17 137.0 18 170.0 19 210.0 20 312.5 21 492.5 22 720.5 23 974.0 24 1391.5 25 2148.0 26 3436.0 27 5469.0 28 7665.5 29 9839.0 30 12706.0 31 16789.0 32 27899.0 33 50369.5 34 86536.0 35 138581.0 36 212637.5 37 305918.5 38 393593.5 39 428447.5 40 435714.5 41 425797.5 42 405320.0 43 416062.0 44 413886.0 45 399038.5 46 397261.5 47 360393.5 48 289224.0 49 237658.5 50 197865.5 51 168151.0 52 147398.0 53 119394.5 54 87099.5 55 59521.0 56 42411.0 57 31768.0 58 25379.0 59 20288.5 60 15666.0 61 12269.0 62 9465.0 63 7302.5 64 5839.0 65 4572.0 66 3316.0 67 2482.0 68 1947.0 69 1411.5 70 977.0 71 725.5 72 546.0 73 396.0 74 291.5 75 216.5 76 159.0 77 100.5 78 63.5 79 43.5 80 28.0 81 21.5 82 15.5 83 14.0 84 12.0 85 5.5 86 2.5 87 3.0 88 2.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15933720679475197 2 0.0 3 0.001921031953577953 4 0.010813550835462994 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.746096587007876E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.3238289761023627E-5 22-23 1.1619144880511812E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.668635621137796E-4 38-39 0.0017428717320767718 40-41 0.005840556826603937 42-43 0.002362559459037402 44-45 0.0011464222948771655 46-47 0.0012703598402692914 48-49 0.003020977668933071 50-51 6.894025962437009E-4 52-53 0.014903489833403153 54-55 0.027692295298553157 56-57 0.02934995996817284 58-59 0.02136373438696772 60-61 0.031487882626187014 62-63 0.005391283224557481 64-65 0.011014949346725198 66-67 0.02938094435452087 68-69 0.0280331235483815 70-71 0.031666042847688196 72-73 0.031758996006732286 74-75 0.0397374754913504 76-77 0.02008562845011142 78-79 0.010767074255940945 80-81 0.01574781436138701 82-83 0.007382030047418505 84-85 0.011355777596553545 86-87 0.034431399329250004 88-89 0.015840767520431103 90-91 0.022215805011538584 92-93 0.049629240832959456 94-95 0.056631712147614574 96-97 0.04129444090533898 98-99 0.035941888163716545 100-101 0.03581020452173741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6454864.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.291225378826127 #Duplication Level Percentage of deduplicated Percentage of total 1 84.76105975806881 26.522774242378805 2 6.630614264853916 4.149600907232068 3 2.206370396954418 2.0712010008081227 4 1.2585877143149657 1.575310073106049 5 0.8021311537755865 1.254983335808486 6 0.5915515386695671 1.110622351182048 7 0.4505215663549114 0.9868160309583311 8 0.3529789721208282 0.8836115656495374 9 0.2585861245638896 0.7282329033209287 >10 2.0867190962124966 12.972268140955473 >50 0.2745887496284239 6.002519007378178 >100 0.2546953979470158 17.73775851925377 >500 0.05094149440177406 10.943788617920184 >1k 0.02035214226448286 10.0347105435413 >5k 2.5136727013491607E-4 0.4488526866700036 >10k+ 5.0262598817117075E-5 2.5769500738367657 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 163847 2.5383493749829587 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6479 0.10037391957444805 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011464222948771655 0.0 0.0 2 0.0013942973856614176 0.0 0.0 3 0.0015027427378795277 0.0 1.549219317401575E-5 4 0.0016421724764456695 0.0 1.549219317401575E-5 5 0.0019984929194480318 0.0 1.549219317401575E-5 6 0.0024477665214944883 0.0 1.549219317401575E-5 7 0.00309843863480315 0.0 1.549219317401575E-5 8 0.0035012356573275597 0.0 1.549219317401575E-5 9 0.004120923384288189 0.0 3.09843863480315E-5 10-11 0.006692627451174804 0.0 3.09843863480315E-5 12-13 0.009031948620451181 0.0 3.09843863480315E-5 14-15 0.011673367556620868 0.0 3.09843863480315E-5 16-17 0.014113387981528348 0.0 3.09843863480315E-5 18-19 0.01706465078117835 0.0 3.09843863480315E-5 20-21 0.020511663762396853 0.0 3.09843863480315E-5 22-23 0.024562872277401972 0.0 3.09843863480315E-5 24-25 0.028962655138822443 0.0 3.09843863480315E-5 26-27 0.03317653168215473 0.0 3.09843863480315E-5 28-29 0.03773123647531536 0.0 3.09843863480315E-5 30-31 0.06574112173393584 0.0 3.09843863480315E-5 32-33 0.07282880011104803 0.0 3.09843863480315E-5 34-35 0.0781968450458445 0.0 3.09843863480315E-5 36-37 0.08447118328132087 0.0 3.09843863480315E-5 38-39 0.0919694047775445 0.0 3.09843863480315E-5 40-41 0.0999323920689886 0.0 3.09843863480315E-5 42-43 0.1089720867860268 0.0 3.09843863480315E-5 44-45 0.11903426625255001 0.0 3.09843863480315E-5 46-47 0.12999499292316616 0.0 3.09843863480315E-5 48-49 0.14257465378046696 0.0 3.09843863480315E-5 50-51 0.15643242057462403 0.0 3.09843863480315E-5 52-53 0.17102606654454688 0.0 3.09843863480315E-5 54-55 0.1866034667810197 0.0 3.09843863480315E-5 56-57 0.20476031718096616 0.0 3.09843863480315E-5 58-59 0.22531845752288507 0.0 3.09843863480315E-5 60-61 0.24743356327879254 0.0 3.09843863480315E-5 62-63 0.2729414593398095 0.0 3.09843863480315E-5 64-65 0.3002851802919473 0.0 3.8730482935039374E-5 66-67 0.32974358561233824 0.0 4.647657952204725E-5 68-69 0.3640510473961961 0.0 4.647657952204725E-5 70-71 0.4018210143544465 0.0 4.647657952204725E-5 72-73 0.4435569827652449 0.0 4.647657952204725E-5 74-75 0.4906067734347308 0.0 4.647657952204725E-5 76-77 0.5415605967840686 0.0 5.422267610905513E-5 78-79 0.5981071018692261 0.0 6.1968772696063E-5 80-81 0.6625546254731316 0.0 6.1968772696063E-5 82-83 0.7331912802500564 0.0 6.1968772696063E-5 84-85 0.8138591301071564 0.0 6.1968772696063E-5 86-87 0.9015371973754985 0.0 6.1968772696063E-5 88-89 0.9979218772076376 0.0 6.1968772696063E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 26965 0.0 65.795784 4 TCGGAAG 27210 0.0 65.60579 3 GAAGAGC 27325 0.0 64.92744 6 GATCGGA 27965 0.0 64.00264 1 ATCGGAA 28205 0.0 63.274548 2 GGAAGAG 30045 0.0 59.223362 5 TTGTGTT 59380 0.0 46.234333 1 GCCGTCT 19835 0.0 44.142105 50-51 ATGCCGT 19800 0.0 44.06968 48-49 TCGTATG 20230 0.0 43.83959 44-45 CGTATGC 20085 0.0 43.828793 46-47 TGCCGTC 19910 0.0 43.808796 50-51 TATGCCG 20040 0.0 43.71967 48-49 CGTCTTC 20120 0.0 43.47112 52-53 CTCGTAT 20065 0.0 43.40721 44-45 TCTCGTA 20100 0.0 43.343273 42-43 CATCTCG 20140 0.0 43.046803 40-41 ATCTCGT 20185 0.0 42.8549 42-43 GTATGCC 20775 0.0 42.475983 46-47 CTGCTTG 20970 0.0 42.207645 58-59 >>END_MODULE