FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071072
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1116713.6362221400214647TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG181350.069.2650453
CGGAAGA181050.068.907674
GATCGGA183050.068.625261
ATCGGAA183350.068.4317862
GAAGAGC192350.064.610546
AGAGCAC192300.064.479178
GAGCACA193050.064.081079
AAGAGCA208650.059.4492847
GGAAGAG223850.055.9853175
GCCGTCT136750.044.44956250-51
ATGCCGT136400.044.35175348-49
CGTCTTC137700.044.1777652-53
TCGTATG139950.044.1508144-45
TGCCGTC136200.043.89657250-51
TCTCGTA136650.043.8608442-43
CGTATGC138350.043.61971746-47
TATGCCG137800.043.590948-49
CTCGTAT136650.043.3228444-45
AATCTCG138500.043.25923540-41
CCGTCTT138600.043.22250452-53
Produced by FastQC (version 0.11.2)