FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035834
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT708272.3330327020515615TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG104000.071.4188163
CGGAAGA104700.070.487744
GATCGGA106300.069.848811
ATCGGAA106800.069.324082
GAAGAGC115600.063.918316
GGAAGAG145200.051.215175
TTGTGTT226850.050.280521
AGAGCAC156900.047.1539468
GAGCACA159050.046.725529
GCCGTCT81800.044.7485450-51
TCTCGTA82950.044.56944342-43
TCGTATG83450.044.5584844-45
ATGCCGT82750.044.34305248-49
TGCCGTC81650.044.27799650-51
TATGCCG82400.044.27248-49
CGTATGC82750.044.19372646-47
ATCTCGT83250.043.9524842-43
CGTCTTC83450.043.9509652-53
CTCGTAT83700.043.74454544-45
TATCTCG85300.043.45577240-41
Produced by FastQC (version 0.11.2)