##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_50331580-1.341000000357a8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3035834 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.682791944487086 33.0 33.0 33.0 33.0 33.0 2 32.665942538360135 33.0 33.0 33.0 33.0 33.0 3 32.73432440640694 33.0 33.0 33.0 33.0 33.0 4 36.68484344005634 37.0 37.0 37.0 37.0 37.0 5 36.690657657829775 37.0 37.0 37.0 37.0 37.0 6 36.57486871811832 37.0 37.0 37.0 37.0 37.0 7 36.517398184485714 37.0 37.0 37.0 37.0 37.0 8 36.461041348110605 37.0 37.0 37.0 37.0 37.0 9 36.47927587608545 37.0 37.0 37.0 37.0 37.0 10-11 36.34860469972996 37.0 37.0 37.0 37.0 37.0 12-13 36.374118446529025 37.0 37.0 37.0 37.0 37.0 14-15 38.26687328753812 40.0 37.0 40.0 37.0 40.0 16-17 38.22584732893827 40.0 37.0 40.0 37.0 40.0 18-19 38.14685091477334 40.0 37.0 40.0 37.0 40.0 20-21 38.15139332387739 40.0 37.0 40.0 37.0 40.0 22-23 38.07877654048278 40.0 37.0 40.0 37.0 40.0 24-25 38.06239735110681 40.0 37.0 40.0 37.0 40.0 26-27 37.97914411657554 40.0 37.0 40.0 35.0 40.0 28-29 37.84606948205995 40.0 37.0 40.0 33.0 40.0 30-31 37.74151221707116 40.0 37.0 40.0 33.0 40.0 32-33 37.64999716717054 40.0 37.0 40.0 33.0 40.0 34-35 37.57967859902748 40.0 37.0 40.0 33.0 40.0 36-37 37.53426455464957 40.0 37.0 40.0 33.0 40.0 38-39 37.40348500609717 40.0 37.0 40.0 33.0 40.0 40-41 37.25633400904002 40.0 37.0 40.0 33.0 40.0 42-43 37.2017906776194 40.0 37.0 40.0 33.0 40.0 44-45 37.107044390437686 40.0 37.0 40.0 33.0 40.0 46-47 37.03660723873571 40.0 37.0 40.0 33.0 40.0 48-49 36.862155177127605 37.0 37.0 40.0 33.0 40.0 50-51 36.743693166358895 37.0 37.0 40.0 33.0 40.0 52-53 36.59058021617783 37.0 37.0 40.0 33.0 40.0 54-55 36.2377427421921 37.0 37.0 40.0 33.0 40.0 56-57 36.12928770150147 37.0 37.0 40.0 33.0 40.0 58-59 35.964808517198236 37.0 37.0 40.0 30.0 40.0 60-61 35.718440468088836 37.0 37.0 40.0 27.0 40.0 62-63 35.50525868673979 37.0 33.0 40.0 27.0 40.0 64-65 35.303574404924646 37.0 33.0 40.0 27.0 40.0 66-67 34.835595753918035 37.0 33.0 38.5 27.0 40.0 68-69 34.59296753379796 37.0 33.0 37.0 27.0 40.0 70-71 34.30491752842876 37.0 33.0 37.0 27.0 40.0 72-73 33.86406832521146 37.0 33.0 37.0 27.0 40.0 74-75 33.37961001820258 37.0 33.0 37.0 24.5 38.5 76-77 30.785713579859767 33.0 30.0 35.0 22.0 37.0 78-79 33.05338582412609 35.0 33.0 37.0 27.0 37.0 80-81 33.34376681992494 37.0 33.0 37.0 27.0 37.0 82-83 33.19433819503965 37.0 33.0 37.0 27.0 37.0 84-85 33.04271659781135 37.0 33.0 37.0 27.0 37.0 86-87 32.89397625166593 37.0 33.0 37.0 27.0 37.0 88-89 32.68972200061005 37.0 33.0 37.0 24.5 37.0 90-91 32.556384176473415 37.0 33.0 37.0 22.0 37.0 92-93 32.373297749481694 33.0 33.0 37.0 22.0 37.0 94-95 32.233608787568755 33.0 33.0 37.0 22.0 37.0 96-97 32.067588840496555 33.0 33.0 37.0 22.0 37.0 98-99 31.93054560954255 33.0 33.0 37.0 22.0 37.0 100-101 30.284128348256196 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12872174362161815 2306 2 0.16389661863100713 2306 3 0.14460365289534138 2306 4 0.19306492512392026 2306 5 0.14309801941429612 2306 6 0.14794639409383592 2306 7 0.12627986430516103 2306 8 0.15036065811010246 2306 9 0.12035638616918476 2306 10-11 0.27303142003675873 2306 12-13 0.1857703716359822 2306 14-15 0.4027002249630982 2306 16-17 0.45125089141280483 2306 18-19 0.5150333994413216 2306 20-21 0.4699852913808371 2306 22-23 0.4738261153011578 2306 24-25 0.4333707811262357 2306 26-27 0.4711528002445746 2306 28-29 0.4962874350750397 2306 30-31 0.5770489717787157 2306 32-33 0.5454110967814643 2306 34-35 0.4650355535002859 2306 36-37 0.4702607570090578 2306 38-39 0.48465010719942114 2306 40-41 0.5792049655123179 2306 42-43 0.5599636185182888 2306 44-45 0.5366220214835664 2306 46-47 0.5234433087294761 2306 48-49 0.4540195897592483 2306 50-51 0.5143458136338594 2306 52-53 0.5563902453978287 2306 54-55 0.6102300832293963 2306 56-57 0.7144472853376271 2306 58-59 0.5383297913210043 2306 60-61 0.5508094144169817 2306 62-63 0.45725028772418597 2306 64-65 0.6665062133217816 2306 66-67 0.6385109326171161 2306 68-69 0.6219167512109678 2306 70-71 0.3977510770174959 2306 72-73 0.3228782057890669 2306 74-75 0.33460058748331534 2306 76-77 0.10232683778096074 2306 78-79 0.26034245466026107 2306 80-81 0.29039322603452433 2306 82-83 0.29775041824111526 2306 84-85 0.4448898306871314 2306 86-87 0.3828986777522019 2306 88-89 0.28455634856944556 2306 90-91 0.3745380878630229 2306 92-93 0.354851619545542 2306 94-95 0.28959577105272416 2306 96-97 0.3629852482054474 2306 98-99 0.3640286955061214 2306 100-101 0.21588655668437795 2307 1 0.09547669839457029 2307 2 0.1947508008082437 2307 3 0.10742182757900309 2307 4 0.16838448179554888 2307 5 0.09916044498028498 2307 6 0.15160057077176248 2307 7 0.16905475483930132 2307 8 0.13533895015820008 2307 9 0.1529743573401987 2307 10-11 0.2206934591998646 2307 12-13 0.20430514550837842 2307 14-15 0.3686953966456201 2307 16-17 0.38683182568822616 2307 18-19 0.4747550618138803 2307 20-21 0.40128440207754323 2307 22-23 0.4675825848665838 2307 24-25 0.4450506082646015 2307 26-27 0.4860342567061693 2307 28-29 0.3091497474469094 2307 30-31 0.4279086494466142 2307 32-33 0.448888378999726 2307 34-35 0.4157676586417409 2307 36-37 0.3704303381507046 2307 38-39 0.44910060300610866 2307 40-41 0.41829955764781346 2307 42-43 0.462300080411417 2307 44-45 0.34596286621189165 2307 46-47 0.3045094912022961 2307 48-49 0.33713520071440684 2307 50-51 0.3119271103290657 2307 52-53 0.4464850149242423 2307 54-55 0.5426738332162699 2307 56-57 0.49074176649398993 2307 58-59 0.4770017958823729 2307 60-61 0.2740567529325233 2307 62-63 0.2415111299090924 2307 64-65 0.2536419150881173 2307 66-67 0.4021642576658522 2307 68-69 0.3233364132669294 2307 70-71 0.2193823059165183 2307 72-73 0.18832833478415978 2307 74-75 0.17506819301707566 2307 76-77 0.17272592535383424 2307 78-79 0.4793181853285162 2307 80-81 0.5637167433547674 2307 82-83 0.5231968122884183 2307 84-85 0.549162947025593 2307 86-87 0.6048983603962057 2307 88-89 0.569556065011291 2307 90-91 0.6689959485437456 2307 92-93 0.5901381498076859 2307 94-95 0.688188406064107 2307 96-97 0.6506635867112536 2307 98-99 0.71087339977597 2307 100-101 0.2955706939066651 2201 1 0.17857307929320854 2201 2 0.24027357692111195 2201 3 0.14599004188593767 2201 4 0.15962595959798165 2201 5 0.1384097673394109 2201 6 0.1703378909557216 2201 7 0.14961595157593877 2201 8 0.2178535298599158 2201 9 0.19893750799058552 2201 10-11 0.27565869067129967 2201 12-13 0.19302320187460964 2201 14-15 0.3104990967848096 2201 16-17 0.29579851560068704 2201 18-19 0.3820360178580984 2201 20-21 0.3667719311055322 2201 22-23 0.40945794003840774 2201 24-25 0.32829802119260876 2201 26-27 0.37171905366560765 2201 28-29 0.4283559843283413 2201 30-31 0.40352667624336647 2201 32-33 0.46057011833532613 2201 34-35 0.5344896889943698 2201 36-37 0.3996325462557522 2201 38-39 0.3791804440055486 2201 40-41 0.4930203470071888 2201 42-43 0.4649126211892991 2201 44-45 0.42663581740445267 2201 46-47 0.46306589826657074 2201 48-49 0.4960168377297052 2201 50-51 0.47433782190891094 2201 52-53 0.44725275964672306 2201 54-55 0.7139119825268665 2201 56-57 0.5352851715514149 2201 58-59 0.3593665763294709 2201 60-61 0.4240544500407921 2201 62-63 0.3952984108582811 2201 64-65 0.3959418755537172 2201 66-67 0.5897674918504165 2201 68-69 0.5029384256347456 2201 70-71 0.3868308713861879 2201 72-73 0.43719645065745283 2201 74-75 0.3861314732286658 2201 76-77 0.30553137997120317 2201 78-79 0.5391671738453923 2201 80-81 0.4768904483377341 2201 82-83 0.4479840259486565 2201 84-85 0.6018936886440542 2201 86-87 0.7282906380554337 2201 88-89 0.623953620829873 2201 90-91 0.5637256310032299 2201 92-93 0.4843088887688296 2201 94-95 0.5617203553909107 2201 96-97 0.7318196315109731 2201 98-99 0.5547839975809836 2201 100-101 0.42942820192440934 1101 1 0.051120135760967855 1101 2 0.13267470518861302 1101 3 0.1033003883757928 1101 4 0.06392207172265074 1101 5 0.08937963488416756 1101 6 0.11599474708431501 1101 7 0.09875986254070312 1101 8 0.15406463751008204 1101 9 0.06042036148885188 1101 10-11 0.12083725680251689 1101 12-13 0.11591910288174034 1101 14-15 0.17041371037049657 1101 16-17 0.09530684534947653 1101 18-19 0.19320857616263254 1101 20-21 0.2967674782312173 1101 22-23 0.19538253102908243 1101 24-25 0.13349095023832547 1101 26-27 0.22098161009133577 1101 28-29 0.26084988441585466 1101 30-31 0.2754932521838853 1101 32-33 0.1419576908297273 1101 34-35 0.16576906066011787 1101 36-37 0.27755750682572256 1101 38-39 0.2017891794263278 1101 40-41 0.3726105002528044 1101 42-43 0.28679401775406177 1101 44-45 0.29009820683968 1101 46-47 0.32718779825025734 1101 48-49 0.3569687300004247 1101 50-51 0.21298221987782995 1101 52-53 0.12065289227501097 1101 54-55 0.32143970406431777 1101 56-57 0.3915585329183884 1101 58-59 0.3028571755423499 1101 60-61 0.30505694624611834 1101 62-63 0.09843391729317119 1101 64-65 0.08160873442633942 1101 66-67 0.18393473637100044 1101 68-69 0.2806843750950847 1101 70-71 0.11223329874346177 1101 72-73 -0.10979883257131462 1101 74-75 0.01280609079443451 1101 76-77 -0.20467276314868954 1101 78-79 0.0768067085710129 1101 80-81 0.17907844430326492 1101 82-83 0.20832583426678752 1101 84-85 0.2709753675229081 1101 86-87 0.23895119306713042 1101 88-89 0.24145588898375792 1101 90-91 0.27195374548133344 1101 92-93 0.015942759908021742 1101 94-95 -0.00446755775919172 1101 96-97 0.1185176969673023 1101 98-99 0.19441922961250668 1101 100-101 0.13290068680750622 2304 1 0.14741574947272795 2304 2 0.20099060986340334 2304 3 0.10453373331306892 2304 4 0.08974438700390408 2304 5 0.05912834138646872 2304 6 0.09490302706228704 2304 7 0.1338947639982493 2304 8 0.08290633705456685 2304 9 0.0019527296476482547 2304 10-11 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74-75 -0.020925886129653293 2304 76-77 -0.17975394804718903 2304 78-79 0.03480735304457738 2304 80-81 0.19663707831508503 2304 82-83 0.22714319682839346 2304 84-85 0.020242371272573223 2304 86-87 0.17429543467019926 2304 88-89 0.1701376077771286 2304 90-91 0.10398632865426549 2304 92-93 0.08181764273101777 2304 94-95 0.04302766983724382 2304 96-97 0.3008029644039212 2304 98-99 0.18567951673031757 2304 100-101 0.19579694135238057 2202 1 0.074169781151042 2202 2 0.07451210996927671 2202 3 0.055984588533398494 2202 4 -0.005983693972119397 2202 5 0.040433614395595896 2202 6 0.05377219651956722 2202 7 0.034089518437738775 2202 8 0.03559865379587279 2202 9 0.046482221220927045 2202 10-11 0.1534564451397813 2202 12-13 0.13061078924896208 2202 14-15 0.08293012749683726 2202 16-17 0.12718397957589644 2202 18-19 0.21345499722908556 2202 20-21 0.16498638419392364 2202 22-23 0.1641374561669835 2202 24-25 0.25337044853094426 2202 26-27 0.09685418035663673 2202 28-29 0.2782503966301988 2202 30-31 0.17386959838824367 2202 32-33 0.24934047254761538 2202 34-35 0.23219849985108 2202 36-37 0.18015633792632713 2202 38-39 0.1683245090822325 2202 40-41 0.39701045428485315 2202 42-43 0.20639360404247498 2202 44-45 0.22472849315436605 2202 46-47 0.2420494018751569 2202 48-49 0.2634625243266626 2202 50-51 0.20860213056866428 2202 52-53 0.2434374010745941 2202 54-55 0.41281989187623935 2202 56-57 0.4922234265354106 2202 58-59 0.44280414822425485 2202 60-61 0.34125260555790504 2202 62-63 0.40965748571974814 2202 64-65 0.24336914288443978 2202 66-67 0.2475962480599989 2202 68-69 0.3428034977638461 2202 70-71 0.18627289685220916 2202 72-73 0.15568580099842677 2202 74-75 0.051754902194481645 2202 76-77 -0.10057751098162626 2202 78-79 0.07938337655104988 2202 80-81 0.24697639544589123 2202 82-83 0.21668625585859047 2202 84-85 0.13635575234035002 2202 86-87 0.3681662902565179 2202 88-89 0.22318198564779124 2202 90-91 0.1111627683339762 2202 92-93 0.04174966166794292 2202 94-95 0.07760658198338177 2202 96-97 0.22263264968597696 2202 98-99 0.19661994539921324 2202 100-101 0.11764178872502384 1102 1 0.09379463472433969 1102 2 0.15315023139438466 1102 3 0.08111288037712683 1102 4 0.07706095207962704 1102 5 0.02380174704470761 1102 6 0.04370784229193703 1102 7 0.00930701416134383 1102 8 0.06498079439490567 1102 9 0.12667013752611922 1102 10-11 0.13495570749230978 1102 12-13 0.059409306111923854 1102 14-15 0.05191609504035455 1102 16-17 0.051759158351252665 1102 18-19 0.1427952316098242 1102 20-21 0.19875912015534425 1102 22-23 0.06115206697829478 1102 24-25 0.13173470914350816 1102 26-27 0.1069391200950065 1102 28-29 0.10263381157037799 1102 30-31 0.17105489739278568 1102 32-33 0.22523263387920167 1102 34-35 0.2080706883359369 1102 36-37 0.16077667248988092 1102 38-39 0.0646226706000661 1102 40-41 0.28829506367478785 1102 42-43 0.11937987546733808 1102 44-45 0.08420873351470703 1102 46-47 -0.09774349318284692 1102 48-49 0.0842817051213629 1102 50-51 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1213 92-93 -1.3549169307607798 1213 94-95 -1.27417525248579 1213 96-97 -1.4700409310081994 1213 98-99 -1.5618942810661167 1213 100-101 -1.5365087311880679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 10.0 10 8.0 11 40.0 12 35.0 13 110.0 14 305.0 15 766.0 16 1450.0 17 2423.0 18 3463.0 19 4688.0 20 6266.0 21 7877.0 22 10170.0 23 12592.0 24 16191.0 25 20626.0 26 26298.0 27 35177.0 28 50728.0 29 70239.0 30 67430.0 31 70902.0 32 93030.0 33 129682.0 34 193873.0 35 320629.0 36 586460.0 37 941042.0 38 361853.0 39 1467.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.3770040949587 25.26726764771478 22.062431294299916 16.293296963026602 2 29.11150609684192 24.978111451416645 31.50172242619326 14.40866002554817 3 36.11139019012912 20.15258686485413 26.978215476159495 16.75780746885725 4 28.682937692596074 20.885007995393178 31.818798760532868 18.613255551477877 5 32.51518363652294 19.0829603990205 31.791428648602 16.610427315854555 6 27.894179984808126 20.308488540546023 35.175935179591505 16.621396295054343 7 32.046778578802396 22.60528737737307 27.172862547820465 18.175071496004065 8 28.47757815480029 30.08955693888401 24.145951326719445 17.28691357959625 9 31.655255195112776 25.187411432904433 24.506741804723184 18.650591567259607 10-11 26.773779462249912 28.988607413975863 27.639488852157267 16.59812427161696 12-13 25.645111030445012 27.91766611744911 27.18645024727966 19.25077260482622 14-15 24.65441127545182 28.382381908892253 27.843419633616328 19.119787182039598 16-17 24.84490258690034 27.597688147639165 28.135398707570968 19.42201055788953 18-19 22.663261561732295 28.09937895155005 29.604154904385418 19.633204582332237 20-21 23.637087415546244 27.98811726468485 29.70084322820129 18.67395209156762 22-23 22.11824912346813 30.979291316899378 28.120005481206444 18.78245407842605 24-25 22.003097007280374 28.018511552344428 29.742963548072787 20.23542789230241 26-27 22.068449724194405 27.93108911752092 28.199466769263402 21.80099438902127 28-29 22.90777427224282 29.78369370657289 28.07365290724065 19.23487911394365 30-31 21.30373399863102 28.482585016176778 29.549738226793693 20.663942758398516 32-33 21.206133141667166 30.00427559609649 28.195843382740954 20.59374787949539 34-35 22.433291807127794 28.597331079367315 28.613916307676902 20.35546080582799 36-37 21.168355675018653 28.55588550648016 29.759602477761256 20.516156340739936 38-39 21.266608438686944 30.05769379318614 28.145673821966334 20.530023946160586 40-41 21.166114201712162 28.379113118743593 30.806263923146293 19.648508756397955 42-43 21.143594264932375 29.72929704723174 29.459626790067478 19.667481897768404 44-45 20.72643706027707 28.301319969648752 30.04274505857537 20.929497911498803 46-47 22.254361012605774 28.152872158710508 28.356048167339555 21.236718661344167 48-49 20.88058114666278 29.98948355786802 29.34041237300181 19.789522922467388 50-51 22.18060418800913 28.29839482708075 29.41867244656286 20.102328538347262 52-53 20.82065222046588 28.46404406207645 28.049347786123136 22.665955931334533 54-55 21.979175916405808 28.44666491268274 29.663091757467186 19.91106741344427 56-57 20.902490498149053 28.244262232963802 29.36675744291064 21.486489825976506 58-59 20.709265610454295 28.582211739867525 29.628254386475017 21.080268263203163 60-61 21.898731507814357 28.41615914787587 29.68791342475756 19.997195919552215 62-63 20.60804739938033 28.691287520981906 29.272233653961926 21.42843142567584 64-65 21.809711071435807 28.542680408839015 29.511343123239257 20.136265396485918 66-67 20.873306718823727 30.833483803282835 27.859631965880006 20.433577512013432 68-69 20.83753503938662 31.088286486473127 27.892990969455294 20.18118750468496 70-71 20.639657394297394 30.98880376320613 28.106412851470203 20.265125991026274 72-73 20.550524828331685 31.148546245868204 27.877823925041422 20.42310500075869 74-75 20.560338826382786 30.757375166517413 28.25542473989506 20.42686126720474 76-77 20.95819589062218 30.17363907005921 28.13860287479853 20.72956216452008 78-79 20.745245362065052 30.21108498860231 28.449899332346888 20.593770316985747 80-81 20.50347079230265 30.067356761867735 28.556829076245414 20.872343369584197 82-83 20.3910314545424 30.208308969566676 28.25506236520317 21.145597210687754 84-85 20.36365289540985 29.94871975640072 28.657044675350857 21.03058267283857 86-87 20.57492285209185 29.616377598850647 28.65751930551231 21.151180243545195 88-89 20.590124313772066 30.175233873859746 28.397988563732575 20.836653248635617 90-91 20.78065185067063 29.600137456455922 28.398348941054834 21.220861751818614 92-93 20.562460966530498 29.898014504384108 28.189835725738277 21.349688803347114 94-95 20.21219232442552 29.957844985563803 28.538120789442463 21.29184190056822 96-97 20.524124531313745 29.945138416092554 28.185122255520266 21.345614797073434 98-99 20.571400138431976 29.90189980668622 28.070271115448346 21.456428939433454 100-101 20.5935139340539 29.61139062752547 28.039228188699035 21.755867249721593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 78.0 1 54.5 2 43.0 3 81.5 4 82.5 5 50.5 6 44.0 7 50.5 8 57.0 9 60.0 10 61.5 11 67.0 12 70.5 13 76.0 14 95.5 15 118.5 16 161.0 17 228.0 18 383.0 19 634.5 20 956.5 21 1454.5 22 2273.0 23 3485.0 24 5050.5 25 7155.0 26 9855.0 27 13263.0 28 17601.5 29 22520.5 30 27969.5 31 34937.0 32 43779.0 33 54484.0 34 67600.0 35 84337.0 36 104808.0 37 129122.5 38 153530.5 39 170539.5 40 179430.5 41 176390.0 42 167621.0 43 163906.0 44 158496.0 45 152563.0 46 150142.5 47 141380.0 48 123821.5 49 107893.5 50 96636.5 51 86041.5 52 74574.5 53 62826.0 54 51940.0 55 41762.0 56 32773.0 57 25685.5 58 20252.0 59 16087.0 60 12549.5 61 9510.0 62 7079.5 63 5225.0 64 3797.5 65 2808.5 66 2098.0 67 1474.0 68 1045.5 69 753.0 70 545.5 71 403.5 72 279.0 73 206.0 74 160.5 75 123.5 76 92.5 77 64.5 78 53.5 79 41.5 80 25.5 81 24.0 82 24.5 83 15.5 84 7.5 85 6.5 86 5.0 87 3.0 88 2.0 89 2.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.157782013114024 2 0.0 3 0.0018116932612257455 4 0.011067798832215463 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.293987747683173E-5 22-23 1.9763926486099042E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.552564468281203E-4 38-39 0.002157561974732479 40-41 0.006472685924197437 42-43 0.0029481190341764407 44-45 0.0014658245477190124 46-47 0.0013999447927653488 48-49 0.0036727963386667386 50-51 7.905570594439617E-4 52-53 0.015811141188879232 54-55 0.027620087264323415 56-57 0.029612949851671733 58-59 0.022415586622983995 60-61 0.031984621030003615 62-63 0.006011527639521792 64-65 0.011874825830397841 66-67 0.0295800099741949 68-69 0.028213005058906383 70-71 0.03211638053991094 72-73 0.032429309375940844 74-75 0.040894857887486605 76-77 0.020752122810403995 78-79 0.011545427055629524 80-81 0.016601698248323197 82-83 0.008136149736777438 84-85 0.01175953625922893 86-87 0.03554212779750145 88-89 0.016157009902385968 90-91 0.02276145533649073 92-93 0.05201206653591731 94-95 0.05909414019343613 96-97 0.04242656219015928 98-99 0.03723853148755828 100-101 0.03717265173260462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3035834.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.11406798388744 #Duplication Level Percentage of deduplicated Percentage of total 1 94.41399157990436 72.80646965322921 2 3.4201685682623446 5.274862229786748 3 0.819503954974816 1.8958585109097776 4 0.41031952510101516 1.2656563101502436 5 0.18606230748733774 0.7174010714408765 6 0.13456428831928666 0.6226079806594143 7 0.09550990917163273 0.515561034029734 8 0.0564398746038951 0.3481846661765479 9 0.04330871151815311 0.30057398318748846 >10 0.34386393932583814 5.0442056401173 >50 0.0368057557395078 2.0514404921148164 >100 0.03726452072171158 5.578205245414961 >500 0.001889431297601717 0.9870600389835859 >1k 2.649328595567598E-4 0.2597034982878517 >5k 0.0 0.0 >10k+ 4.270071295285205E-5 2.3322096455115746 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 70827 2.3330327020515615 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010870159567354474 0.0 0.0 2 0.0011528957116891108 0.0 0.0 3 0.0014164147315037646 0.0 0.0 4 0.0016469938738415868 3.293987747683174E-5 0.0 5 0.0018116932612257455 3.293987747683174E-5 0.0 6 0.0020752122810403996 3.293987747683174E-5 3.293987747683174E-5 7 0.002635190198146539 3.293987747683174E-5 3.293987747683174E-5 8 0.0030963484828221833 3.293987747683174E-5 3.293987747683174E-5 9 0.003524566890020996 3.293987747683174E-5 3.293987747683174E-5 10-11 0.005089211070170503 3.293987747683174E-5 4.940981621524761E-5 12-13 0.00645621598545902 3.293987747683174E-5 1.3175950990732696E-4 14-15 0.007905570594439618 3.293987747683174E-5 1.3175950990732696E-4 16-17 0.009239635632251303 3.293987747683174E-5 1.3175950990732696E-4 18-19 0.011084268770953878 3.293987747683174E-5 1.3175950990732696E-4 20-21 0.012665382889841804 3.293987747683174E-5 1.3175950990732696E-4 22-23 0.014938234435743193 3.293987747683174E-5 1.4822944864574284E-4 24-25 0.01717814610416775 3.293987747683174E-5 1.8116932612257454E-4 26-27 0.019632166976191717 3.293987747683174E-5 1.9763926486099042E-4 28-29 0.021756789073447365 3.293987747683174E-5 1.9763926486099042E-4 30-31 0.0349821498803953 3.293987747683174E-5 2.3057914233782215E-4 32-33 0.038490246831677885 3.293987747683174E-5 2.3057914233782215E-4 34-35 0.04105955727487076 3.293987747683174E-5 2.3057914233782215E-4 36-37 0.04417237569643136 3.293987747683174E-5 2.3057914233782215E-4 38-39 0.047762822341406025 3.293987747683174E-5 2.3057914233782215E-4 40-41 0.05132032910890384 3.293987747683174E-5 2.3057914233782215E-4 42-43 0.05556957330341514 3.293987747683174E-5 2.3057914233782215E-4 44-45 0.060098806456479505 3.293987747683174E-5 2.47049081076238E-4 46-47 0.06541859666898783 3.293987747683174E-5 2.635190198146539E-4 48-49 0.07106778565626447 3.293987747683174E-5 2.635190198146539E-4 50-51 0.07709578323452468 3.293987747683174E-5 2.635190198146539E-4 52-53 0.08378257836232153 3.293987747683174E-5 2.635190198146539E-4 54-55 0.09119405079460866 3.293987747683174E-5 2.635190198146539E-4 56-57 0.10127365330251917 6.587975495366348E-5 2.635190198146539E-4 58-59 0.11227557237978097 6.587975495366348E-5 2.635190198146539E-4 60-61 0.12312926200839702 6.587975495366348E-5 2.635190198146539E-4 62-63 0.1351358473487022 6.587975495366348E-5 2.635190198146539E-4 64-65 0.14897059588897155 6.587975495366348E-5 2.635190198146539E-4 66-67 0.1644852781805593 6.587975495366348E-5 2.7998895855306977E-4 68-69 0.1817622439171575 6.587975495366348E-5 3.129288360299015E-4 70-71 0.20226731764648526 6.587975495366348E-5 3.4586871350673323E-4 72-73 0.22591814967485047 6.587975495366348E-5 3.9527852972198084E-4 74-75 0.252237111778839 6.587975495366348E-5 3.9527852972198084E-4 76-77 0.28203123095663335 6.587975495366348E-5 4.282184071988126E-4 78-79 0.314691119474912 6.587975495366348E-5 4.282184071988126E-4 80-81 0.3522096399210234 6.587975495366348E-5 4.282184071988126E-4 82-83 0.393582126031924 6.587975495366348E-5 4.282184071988126E-4 84-85 0.4423496146363734 6.587975495366348E-5 5.270380396293078E-4 86-87 0.49839681616320264 6.587975495366348E-5 5.270380396293078E-4 88-89 0.5583144532935596 6.587975495366348E-5 5.270380396293078E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 10400 0.0 71.418816 3 CGGAAGA 10470 0.0 70.48774 4 GATCGGA 10630 0.0 69.84881 1 ATCGGAA 10680 0.0 69.32408 2 GAAGAGC 11560 0.0 63.91831 6 GGAAGAG 14520 0.0 51.21517 5 TTGTGTT 22685 0.0 50.28052 1 AGAGCAC 15690 0.0 47.153946 8 GAGCACA 15905 0.0 46.72552 9 GCCGTCT 8180 0.0 44.74854 50-51 TCTCGTA 8295 0.0 44.569443 42-43 TCGTATG 8345 0.0 44.55848 44-45 ATGCCGT 8275 0.0 44.343052 48-49 TGCCGTC 8165 0.0 44.277996 50-51 TATGCCG 8240 0.0 44.272 48-49 CGTATGC 8275 0.0 44.193726 46-47 ATCTCGT 8325 0.0 43.95248 42-43 CGTCTTC 8345 0.0 43.95096 52-53 CTCGTAT 8370 0.0 43.744545 44-45 TATCTCG 8530 0.0 43.455772 40-41 >>END_MODULE