##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_50330665-2.34100000035758.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2395603 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68862703878731 33.0 33.0 33.0 33.0 33.0 2 32.66970612409485 33.0 33.0 33.0 33.0 33.0 3 32.73757337922853 33.0 33.0 33.0 33.0 33.0 4 36.69967853605126 37.0 37.0 37.0 37.0 37.0 5 36.705981333301054 37.0 37.0 37.0 37.0 37.0 6 36.598181334720316 37.0 37.0 37.0 37.0 37.0 7 36.55557869980961 37.0 37.0 37.0 37.0 37.0 8 36.48860057363428 37.0 37.0 37.0 37.0 37.0 9 36.49624541295031 37.0 37.0 37.0 37.0 37.0 10-11 36.37867793620228 37.0 37.0 37.0 37.0 37.0 12-13 36.4158209853636 37.0 37.0 37.0 37.0 37.0 14-15 38.329738692095475 40.0 37.0 40.0 37.0 40.0 16-17 38.30019811295945 40.0 37.0 40.0 37.0 40.0 18-19 38.25832702664006 40.0 37.0 40.0 37.0 40.0 20-21 38.26242703820291 40.0 37.0 40.0 37.0 40.0 22-23 38.20621154673792 40.0 37.0 40.0 37.0 40.0 24-25 38.19956311625925 40.0 37.0 40.0 37.0 40.0 26-27 38.13556962485019 40.0 37.0 40.0 37.0 40.0 28-29 38.03555576612652 40.0 37.0 40.0 37.0 40.0 30-31 37.938874679986625 40.0 37.0 40.0 35.0 40.0 32-33 37.86172270614121 40.0 37.0 40.0 33.0 40.0 34-35 37.806911871457835 40.0 37.0 40.0 33.0 40.0 36-37 37.75419028111085 40.0 37.0 40.0 33.0 40.0 38-39 37.64779180857596 40.0 37.0 40.0 33.0 40.0 40-41 37.50342335520535 40.0 37.0 40.0 33.0 40.0 42-43 37.484924463694526 40.0 37.0 40.0 33.0 40.0 44-45 37.374108940421266 40.0 37.0 40.0 33.0 40.0 46-47 37.32013609934535 40.0 37.0 40.0 33.0 40.0 48-49 37.188851825615515 40.0 37.0 40.0 33.0 40.0 50-51 37.052045351420915 37.0 37.0 40.0 33.0 40.0 52-53 36.901430454044345 37.0 37.0 40.0 33.0 40.0 54-55 36.58899032936593 37.0 37.0 40.0 33.0 40.0 56-57 36.479696969823465 37.0 37.0 40.0 33.0 40.0 58-59 36.3464056857501 37.0 37.0 40.0 33.0 40.0 60-61 36.091786493838924 37.0 37.0 40.0 33.0 40.0 62-63 35.878117743215384 37.0 37.0 40.0 33.0 40.0 64-65 35.6690044218512 37.0 35.0 40.0 33.0 40.0 66-67 35.253573526164395 37.0 33.0 38.5 27.0 40.0 68-69 35.0344618870489 37.0 33.0 37.0 27.0 40.0 70-71 34.810116075159364 37.0 33.0 37.0 27.0 40.0 72-73 34.509703611157605 37.0 33.0 37.0 27.0 40.0 74-75 34.15037591787955 37.0 33.0 37.0 27.0 38.5 76-77 31.480494890013077 33.0 30.0 35.0 24.5 37.0 78-79 33.84568999955334 37.0 33.0 37.0 27.0 37.0 80-81 34.17966916888984 37.0 33.0 37.0 27.0 37.0 82-83 34.071125098774715 37.0 33.0 37.0 27.0 37.0 84-85 33.94631017743758 37.0 33.0 37.0 27.0 37.0 86-87 33.810954486198256 37.0 33.0 37.0 27.0 37.0 88-89 33.65217671709378 37.0 33.0 37.0 27.0 37.0 90-91 33.54525875113698 37.0 33.0 37.0 27.0 37.0 92-93 33.379079922675004 37.0 33.0 37.0 27.0 37.0 94-95 33.26512844573996 37.0 33.0 37.0 27.0 37.0 96-97 33.107335814824076 37.0 33.0 37.0 27.0 37.0 98-99 32.98512712665663 35.0 33.0 37.0 27.0 37.0 100-101 31.44714566645642 33.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13500978065737712 2306 2 0.2056106479800519 2306 3 0.16072454701876637 2306 4 0.1746062136683051 2306 5 0.12542679432171155 2306 6 0.09994141769038833 2306 7 0.03550518169234351 2306 8 0.1606323286166429 2306 9 0.202233602599442 2306 10-11 0.2515104178098966 2306 12-13 0.1829897656300048 2306 14-15 0.2885262379868294 2306 16-17 0.4513861435945046 2306 18-19 0.4259196664369824 2306 20-21 0.43030784152692547 2306 22-23 0.4236445302739966 2306 24-25 0.38847602407241055 2306 26-27 0.3722563047398424 2306 28-29 0.31524062696100685 2306 30-31 0.33513792980564716 2306 32-33 0.3773536729173088 2306 34-35 0.3375899240370259 2306 36-37 0.3029324268413447 2306 38-39 0.37369421557356475 2306 40-41 0.48423110341825293 2306 42-43 0.3525504975652538 2306 44-45 0.35157128441279184 2306 46-47 0.2909296963162191 2306 48-49 0.34421615890623514 2306 50-51 0.4814643568319781 2306 52-53 0.34991185342934017 2306 54-55 0.47176336952270503 2306 56-57 0.4097684128772272 2306 58-59 0.2632339635772851 2306 60-61 0.3780146428696156 2306 62-63 0.3770720972772139 2306 64-65 0.3987235462008414 2306 66-67 0.4028703329229941 2306 68-69 0.2815387036757784 2306 70-71 0.16929262569752268 2306 72-73 0.06814226768420184 2306 74-75 0.18999492222588543 2306 76-77 -0.08888325580866407 2306 78-79 0.2565929818348067 2306 80-81 0.2126472350111399 2306 82-83 0.19216774704445783 2306 84-85 0.32048109216071197 2306 86-87 0.5038807120312256 2306 88-89 0.28839175465622446 2306 90-91 0.3665950400783089 2306 92-93 0.26710695488538505 2306 94-95 0.16430819050453493 2306 96-97 0.33421188276964386 2306 98-99 0.5684553844244462 2306 100-101 0.43446768128696434 2307 1 0.15543651214402132 2307 2 0.22574450797566215 2307 3 0.19737146385001836 2307 4 0.1585413261748485 2307 5 0.1426741399100706 2307 6 0.16621477566881282 2307 7 0.17658399535179825 2307 8 0.21625427963029864 2307 9 0.23180771512286213 2307 10-11 0.2380429712779346 2307 12-13 0.24883900855298435 2307 14-15 0.3365242461230835 2307 16-17 0.3750070311554836 2307 18-19 0.41971788051169767 2307 20-21 0.46108002633137346 2307 22-23 0.4592284164339233 2307 24-25 0.49239012072843735 2307 26-27 0.4331659787831512 2307 28-29 0.4487355882209485 2307 30-31 0.5119449553453919 2307 32-33 0.5690245596342862 2307 34-35 0.5977446310811345 2307 36-37 0.5422215171672988 2307 38-39 0.4987199578804109 2307 40-41 0.5436869701321925 2307 42-43 0.42914272254457586 2307 44-45 0.4466743380411913 2307 46-47 0.5867970010544994 2307 48-49 0.6462451590919187 2307 50-51 0.5515838686565644 2307 52-53 0.6372095020173205 2307 54-55 0.7039062266655591 2307 56-57 0.5614222505909652 2307 58-59 0.44558993326044316 2307 60-61 0.1740414655093403 2307 62-63 0.3113903903512707 2307 64-65 0.3034251042434164 2307 66-67 0.39804850344679465 2307 68-69 0.28715770065422674 2307 70-71 0.26103820730485694 2307 72-73 0.22861896456642938 2307 74-75 0.1757345282419891 2307 76-77 -0.1043210438270954 2307 78-79 0.2596740405610234 2307 80-81 0.21726481405268316 2307 82-83 0.20372815284449786 2307 84-85 0.3082990729510371 2307 86-87 0.2872921162951627 2307 88-89 0.13789927478585895 2307 90-91 0.3251482380324262 2307 92-93 0.44634177536444497 2307 94-95 0.41124696601473687 2307 96-97 0.6388940285808147 2307 98-99 0.39886169086762635 2307 100-101 0.05748889969988724 2201 1 0.126166243242416 2201 2 0.17684485594894994 2201 3 0.1530471903618178 2201 4 0.15497550424167628 2201 5 0.1045327224072281 2201 6 0.15650118445423544 2201 7 0.15105425846590492 2201 8 0.18356721685765365 2201 9 0.19407033729331857 2201 10-11 0.2033568706767639 2201 12-13 0.21462241869123488 2201 14-15 0.26248153439110666 2201 16-17 0.25303823300169626 2201 18-19 0.44729964700064073 2201 20-21 0.3933787841897072 2201 22-23 0.3388048801282295 2201 24-25 0.23969079569534557 2201 26-27 0.1962601920090279 2201 28-29 0.19337473774817937 2201 30-31 0.35797563631682294 2201 32-33 0.45694550965200165 2201 34-35 0.38875610479504275 2201 36-37 0.4910276649365741 2201 38-39 0.5444911057582047 2201 40-41 0.5648433483162165 2201 42-43 0.3818993799753585 2201 44-45 0.4415129753748843 2201 46-47 0.4061386370353688 2201 48-49 0.40927932703063163 2201 50-51 0.4333594005637593 2201 52-53 0.417015837880264 2201 54-55 0.6306554978025858 2201 56-57 0.5614690931493342 2201 58-59 0.42047400244997135 2201 60-61 0.4696084232389097 2201 62-63 0.5774608241965566 2201 64-65 0.35635231199287887 2201 66-67 0.5010724709210521 2201 68-69 0.5545221827817031 2201 70-71 0.5239573487295885 2201 72-73 0.3628458634082037 2201 74-75 0.37600075312968073 2201 76-77 0.10761820191728333 2201 78-79 0.3403150420874752 2201 80-81 0.45997473744068174 2201 82-83 0.5451803806693363 2201 84-85 0.39866379381279415 2201 86-87 0.461606659553091 2201 88-89 0.5369340287086999 2201 90-91 0.4324842529062991 2201 92-93 0.2287687624655561 2201 94-95 0.28515367155409166 2201 96-97 0.28591256304174806 2201 98-99 0.28968959239335135 2201 100-101 0.2381119961557694 1101 1 0.11302900454126785 1101 2 0.12937610558009283 1101 3 0.061258849808709215 1101 4 0.08017232675321395 1101 5 0.03064313186887091 1101 6 0.08711815073178286 1101 7 0.08954412520496646 1101 8 0.06499212921703901 1101 9 0.040125034475835264 1101 10-11 0.16549730811257746 1101 12-13 0.07269066623316434 1101 14-15 0.1711806762803647 1101 16-17 0.19756202285309143 1101 18-19 0.3128595436024284 1101 20-21 0.33732538538657764 1101 22-23 0.2935280302326859 1101 24-25 0.3049402615892731 1101 26-27 0.21829723123189382 1101 28-29 0.22348651640990624 1101 30-31 0.3831948853813998 1101 32-33 0.23525667217369062 1101 34-35 0.21401312489631863 1101 36-37 0.2542847086447537 1101 38-39 0.3959848322250821 1101 40-41 0.3735601953789285 1101 42-43 0.41834961073278265 1101 44-45 0.35035257830686106 1101 46-47 0.38249514840799037 1101 48-49 0.34296991141938804 1101 50-51 0.34076370410125634 1101 52-53 0.4480128752145802 1101 54-55 0.5888830919055792 1101 56-57 0.4564785949257555 1101 58-59 0.42616326485244826 1101 60-61 0.3404881342904744 1101 62-63 0.29601230493922515 1101 64-65 0.16601815635259243 1101 66-67 0.5187410536809338 1101 68-69 0.49952873369588957 1101 70-71 0.3376021357363541 1101 72-73 0.24458474751014592 1101 74-75 0.34215554355943567 1101 76-77 0.14581888415663258 1101 78-79 0.40466508517005906 1101 80-81 0.47702245878486593 1101 82-83 0.4667181340008568 1101 84-85 0.2807402565552124 1101 86-87 0.4804650299689257 1101 88-89 0.28526443085220166 1101 90-91 0.3375691787216155 1101 92-93 0.20622535528197972 1101 94-95 0.24077270343520496 1101 96-97 0.18848217388203636 1101 98-99 0.3223910477586074 1101 100-101 0.3158565931270054 2304 1 0.12175783335278112 2304 2 0.19209768992673304 2304 3 0.13750075599178047 2304 4 0.11581919432885002 2304 5 0.0508845907333253 2304 6 0.09294756274659477 2304 7 0.09699273743971304 2304 8 0.12169034699014958 2304 9 0.10751958406150663 2304 10-11 0.04654710317890931 2304 12-13 0.05376786538955258 2304 14-15 0.16974887810027894 2304 16-17 0.14420937560634428 2304 18-19 0.2912566169271571 2304 20-21 0.18988919672761995 2304 22-23 0.1308356846813794 2304 24-25 0.18049875461477427 2304 26-27 0.06248998129203187 2304 28-29 0.2737460835242942 2304 30-31 0.368071526204929 2304 32-33 0.3636567742182564 2304 34-35 0.26868719678298447 2304 36-37 0.30952346204696113 2304 38-39 0.22781181427468056 2304 40-41 0.44090640193645925 2304 42-43 0.3180087050174123 2304 44-45 0.261394125936107 2304 46-47 0.39164901727723844 2304 48-49 0.3132185634934501 2304 50-51 0.34410796412384315 2304 52-53 0.3330150040551558 2304 54-55 0.4658649376424009 2304 56-57 0.37251272510363265 2304 58-59 0.3643581594792096 2304 60-61 0.4074060973365974 2304 62-63 0.30072953608355135 2304 64-65 0.2703833279383119 2304 66-67 0.2765822346020954 2304 68-69 0.13029633361597348 2304 70-71 0.15151409434540852 2304 72-73 0.0691359582117812 2304 74-75 0.1766597392100877 2304 76-77 0.1319051199646033 2304 78-79 0.3345889536374216 2304 80-81 0.49847147407232484 2304 82-83 0.20378375296344586 2304 84-85 0.3153256160729896 2304 86-87 0.3267972502477221 2304 88-89 0.3134210412953493 2304 90-91 0.37670766518877485 2304 92-93 0.34208280625312426 2304 94-95 0.38531544537022455 2304 96-97 0.4221200974150108 2304 98-99 0.3216656111133105 2304 100-101 0.3369082345475398 2202 1 0.08780319687443949 2202 2 0.1578449899927179 2202 3 0.04365128093072457 2202 4 0.02009452393494371 2202 5 0.028292538320457084 2202 6 0.05300850659491374 2202 7 0.08410945098626144 2202 8 0.12520344441928444 2202 9 0.049915203975622546 2202 10-11 0.16925809159205585 2202 12-13 0.12820342580672417 2202 14-15 0.1262960010644747 2202 16-17 0.1605522238196997 2202 18-19 0.19794249855834778 2202 20-21 0.18311982839063745 2202 22-23 0.1826640001309059 2202 24-25 0.1303068943515555 2202 26-27 0.02952720160433131 2202 28-29 0.08815094051171712 2202 30-31 0.06618316399127622 2202 32-33 0.07043757314406918 2202 34-35 0.10855911631163906 2202 36-37 0.06638832644255643 2202 38-39 0.03688914871923288 2202 40-41 0.1257267246552658 2202 42-43 0.08133402246946275 2202 44-45 0.2213812773754924 2202 46-47 0.08253478662820868 2202 48-49 0.06154159482773025 2202 50-51 0.11044140742233566 2202 52-53 0.10961355020000241 2202 54-55 0.22857097762341283 2202 56-57 0.35909986959784135 2202 58-59 0.19367125343575964 2202 60-61 0.22060495485647635 2202 62-63 0.11427203472344871 2202 64-65 0.1682285895975113 2202 66-67 0.1572461637150795 2202 68-69 0.14152580196336828 2202 70-71 0.3393772687501482 2202 72-73 0.12076170626423277 2202 74-75 0.15930839474133052 2202 76-77 -0.06430744847423497 2202 78-79 0.2569282308769445 2202 80-81 0.34427500106009035 2202 82-83 0.2911561355032859 2202 84-85 0.32877826160319756 2202 86-87 0.4065842072224086 2202 88-89 0.2915350809522579 2202 90-91 0.242731759133747 2202 92-93 0.23858244163181297 2202 94-95 0.20818183191511963 2202 96-97 0.30409420351403327 2202 98-99 0.4474353672221625 2202 100-101 0.2961241829680965 1102 1 0.09404617872176146 1102 2 0.11023229045147076 1102 3 0.0994649916502297 1102 4 0.12665053655209846 1102 5 0.09453402484277973 1102 6 0.06265970087994788 1102 7 0.04252530933102605 1102 8 0.03168920497542871 1102 9 0.07284163962868462 1102 10-11 0.14687124255216588 1102 12-13 0.11314966464116338 1102 14-15 0.11311421750853157 1102 16-17 0.17148572982075194 1102 18-19 0.28687765788098574 1102 20-21 0.23968437564573009 1102 22-23 0.05111489986513362 1102 24-25 0.16054278892267604 1102 26-27 0.12303826700928511 1102 28-29 0.1422972227952144 1102 30-31 0.17554749569385564 1102 32-33 0.1629347094556408 1102 34-35 0.23804226992384514 1102 36-37 0.14760245980297526 1102 38-39 0.1097100652123899 1102 40-41 0.0892630130893437 1102 42-43 0.1219663051253903 1102 44-45 0.060719892309556656 1102 46-47 0.03440048669423135 1102 48-49 0.13759227223816595 1102 50-51 0.18998529097883932 1102 52-53 0.15445284508373902 1102 54-55 0.28226901507989766 1102 56-57 0.17675768281200277 1102 58-59 0.07056968136955533 1102 60-61 0.21520168263398176 1102 62-63 0.09151911319698769 1102 64-65 0.15629420332967214 1102 66-67 0.13638250067669588 1102 68-69 0.26123573712833803 1102 70-71 0.15096385088862974 1102 72-73 -0.025661645633697105 1102 74-75 -0.1065335860846588 1102 76-77 -0.16821560730596374 1102 78-79 0.10811482768654201 1102 80-81 0.2530217353267332 1102 82-83 0.3082102465184704 1102 84-85 0.22538676577035943 1102 86-87 0.3116021511223295 1102 88-89 0.20205680830654416 1102 90-91 0.11339355329803169 1102 92-93 0.2538135963048447 1102 94-95 0.16394701510653675 1102 96-97 0.13175729268618142 1102 98-99 0.1698755401155907 1102 100-101 0.2028490544547914 2305 1 0.10258347680250779 2305 2 0.11400067065578412 2305 3 0.0734229597171776 2305 4 0.07256539734177636 2305 5 0.05399822289313505 2305 6 1.6817505999711102E-4 2305 7 -0.02141091808090323 2305 8 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-1.375629460924145 1213 90-91 -1.4191415192111592 1213 92-93 -1.4693451430069757 1213 94-95 -1.5010995551312405 1213 96-97 -1.5637157466723153 1213 98-99 -1.6623550024861125 1213 100-101 -1.5398105156671242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 11.0 9 24.0 10 15.0 11 11.0 12 22.0 13 54.0 14 136.0 15 350.0 16 689.0 17 1128.0 18 1849.0 19 2441.0 20 3262.0 21 4399.0 22 5537.0 23 7156.0 24 9182.0 25 11725.0 26 15387.0 27 20408.0 28 27585.0 29 33769.0 30 36978.0 31 46407.0 32 62597.0 33 89338.0 34 138096.0 35 244158.0 36 501847.0 37 852732.0 38 277968.0 39 342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.024964093601234 25.932719666671545 23.598626044526025 13.44369019520119 2 31.46080548404723 22.915608304047037 33.55835670601515 12.065229505890583 3 38.52645323633194 18.804525191739575 27.09316833968743 15.575853232241052 4 30.695574595389612 18.91786055276749 34.63119262531368 15.755372226529216 5 30.592172409201357 17.393950500145476 36.56828781730529 15.44558927334788 6 25.484731819086885 18.281618448465796 40.79064853400167 15.443001198445652 7 34.16041806593162 19.04447439746903 29.696948951892278 17.098158584707065 8 30.231219446627843 28.674492392938227 24.81162362878991 16.282664531644016 9 29.793834788151457 26.645191210730662 25.770338407490723 17.790635593627158 10-11 24.215009749111186 28.992387302904532 30.804916340478783 15.9876866075055 12-13 24.227887508906942 27.712959952045473 29.967632366464724 18.091520172582854 14-15 22.90844935492233 28.404497740234923 30.991028146149425 17.696024758693323 16-17 21.83387648120327 29.10640869960507 31.9389314506619 17.12078336852976 18-19 20.45787636766192 29.391827443862777 32.30082363396606 17.84947255450924 20-21 21.099038988947246 29.32826487872359 31.820060260427237 17.752635871901926 22-23 20.32937044386157 30.144759111546353 31.62958514994466 17.89628529464742 24-25 20.40244147298196 29.212728486314305 31.78341736923856 18.60141267146518 26-27 20.30584783872787 29.326436809437954 31.0131728838209 19.35454246801327 28-29 20.408035054222257 29.90061374944012 30.73242937164463 18.95892182469299 30-31 19.45336936044912 30.181440747903554 30.886586800901483 19.47860309074584 32-33 19.26080823909471 30.820736991897242 31.094342426520587 18.824112342487464 34-35 19.852183354253604 29.997144768978835 31.693314793811826 18.457357082955735 36-37 20.161768352294594 30.30356664298422 31.397056920519656 18.137608084201528 38-39 20.724098216741897 30.496138897017357 30.637108082080218 18.142654804160532 40-41 20.398497400382436 30.233440627089248 30.563483205754583 18.804578766773737 42-43 19.836291164369023 30.673626563769616 31.232938299547886 18.257143972313475 44-45 19.546884173840155 30.20994807704309 31.49603110910834 18.747136640008417 46-47 20.314981468915846 29.835348129119193 30.873869449414848 18.975800952550117 48-49 19.976673113425527 30.761622011764466 30.601928016434016 18.65977685837599 50-51 20.496129545029522 30.10048906654912 30.18729462210467 19.21608676631669 52-53 19.91666771040777 29.895229268170485 29.978227560610044 20.209875460811695 54-55 20.683681277212745 30.027005274745004 30.169036108985647 19.120277339056603 56-57 20.16017453846997 30.286474834412893 30.113209910695176 19.44014071642196 58-59 20.14437105088622 30.306104789467238 30.583319085366096 18.966205074280445 60-61 20.44532578708059 30.669825971018415 30.270987806655253 18.613860435245744 62-63 19.91166235574844 30.59802927862113 30.16961612511626 19.32069224051417 64-65 20.413435883434218 30.227961476497683 30.2957813099477 19.062821330120396 66-67 20.444701011642607 30.480621579273176 29.756677561493483 19.317999847590734 68-69 20.37695031263251 30.741789495155235 29.949273551408833 18.931986640803423 70-71 19.754048629063607 31.36158705674699 30.019274910922434 18.865089403266964 72-73 19.596907310770924 31.432339698071953 29.901006719116225 19.069746272040902 74-75 20.134234634155916 30.62634755595163 29.745307488099332 19.494110321793126 76-77 20.116103420892436 30.27287727557778 30.000592875638908 19.61042642789087 78-79 19.875766543969533 30.36559904714551 30.353742638276408 19.404891770608547 80-81 19.366218278609868 30.68817153572579 30.15995051789833 19.785659667766016 82-83 18.84425855157748 30.591807818799822 30.095691502818312 20.46824212680439 84-85 18.883018417360674 30.382831699210318 30.59138407933966 20.142765804089347 86-87 19.00684348755835 30.392688866121574 31.113429600521304 19.487038045798773 88-89 19.16601640863008 30.756745879159304 30.731424434588632 19.345813277621982 90-91 19.466147097328022 30.506703275345238 30.525992975960314 19.50115665136643 92-93 18.863266983952567 31.472155207391673 30.335008721499037 19.329569087156727 94-95 18.662427817852574 31.662012021952187 30.305402193255503 19.370157966939736 96-97 19.173277958220186 31.671766731291846 30.033795105817205 19.121160204670762 98-99 19.417272408769122 30.93917830093777 29.732315372020373 19.911233918272732 100-101 19.263546468898245 30.97228486002539 29.46876879134095 20.295399879735417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 104.0 1 75.0 2 62.5 3 182.0 4 229.5 5 141.5 6 113.5 7 111.5 8 114.5 9 120.5 10 123.0 11 136.5 12 141.0 13 155.0 14 180.0 15 194.5 16 221.0 17 278.0 18 401.0 19 610.0 20 923.0 21 1421.5 22 2261.5 23 3470.5 24 5158.0 25 7653.0 26 11148.5 27 15742.0 28 21024.5 29 26750.0 30 33028.0 31 39773.0 32 48132.0 33 60142.5 34 75470.0 35 94293.0 36 121376.5 37 151289.5 38 175709.5 39 184904.0 40 178199.5 41 162120.0 42 144263.0 43 134024.0 44 120975.5 45 105873.0 46 99027.0 47 88253.5 48 68528.0 49 51407.0 50 38843.0 51 30643.5 52 25387.5 53 19535.0 54 14023.0 55 9793.5 56 6660.0 57 4514.0 58 3164.5 59 2259.5 60 1534.0 61 1023.5 62 695.5 63 482.0 64 328.0 65 213.5 66 144.0 67 93.5 68 65.5 69 50.0 70 42.0 71 33.0 72 22.0 73 16.5 74 12.5 75 14.0 76 10.5 77 5.0 78 4.0 79 2.0 80 1.5 81 0.5 82 1.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1656367937425358 2 0.0 3 0.001502753169035103 4 0.011437621342100506 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.174314358430842E-5 22-23 1.0435785896077106E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.09633440933243E-4 38-39 0.0018366983177095703 40-41 0.00644931568377565 42-43 0.002901148479109435 44-45 0.0014401384536586405 46-47 0.001398395310074332 48-49 0.003506424061081907 50-51 7.9311972810186E-4 52-53 0.01557019255694704 54-55 0.029241072080808048 56-57 0.029867219234572674 58-59 0.021894278809969768 60-61 0.032225706847086096 62-63 0.005635324383881637 64-65 0.011938539065112207 66-67 0.030702082106258844 68-69 0.02915758579363943 70-71 0.032559651995760566 72-73 0.033144056005940885 74-75 0.04132571214846533 76-77 0.020746342361401285 78-79 0.011437621342100506 80-81 0.01642592700042536 82-83 0.00822339928610876 84-85 0.01175069491898282 86-87 0.03525208475694846 88-89 0.01669725743372337 90-91 0.02264565539448732 92-93 0.05144842446766013 94-95 0.05841952944623963 96-97 0.04280759374570828 98-99 0.03681745264136002 100-101 0.036859195784944336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2395603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.03471402216513 #Duplication Level Percentage of deduplicated Percentage of total 1 91.6516857897117 54.10631060244967 2 4.143759427249892 4.892513055286963 3 1.3126740564927144 2.3248001258808855 4 0.6607876233900026 1.5603763350485986 5 0.42835716320243183 1.2643971314500742 6 0.2438334265580635 0.8636781963539931 7 0.17309170660721346 0.7152893579415751 8 0.13460283292357778 0.635699179857335 9 0.1046113048225792 0.5558128617317857 >10 0.9114094688169615 10.717621766547706 >50 0.11562232580351783 4.7637349575931465 >100 0.11338540058895702 13.408601664364003 >500 0.004956797832135324 1.946249508532875 >1k 0.0010087377454317072 0.8652376881434989 >5k 1.426255030435511E-4 0.5751182493556302 >10k+ 7.131275151999495E-5 0.8045593194624081 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 19111 0.7977532170397181 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7240 0.30222035955039295 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6421 0.26803272495484437 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3289 0.13729319924879038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.174314358430842E-4 0.0 0.0 2 4.591745794273926E-4 0.0 0.0 3 5.426608665960094E-4 0.0 4.174314358430842E-5 4 5.426608665960094E-4 0.0 4.174314358430842E-5 5 6.261471537646263E-4 0.0 4.174314358430842E-5 6 6.261471537646263E-4 0.0 4.174314358430842E-5 7 7.513765845175515E-4 0.0 4.174314358430842E-5 8 9.600923024390936E-4 0.0 4.174314358430842E-5 9 0.001001835446023402 0.0 4.174314358430842E-5 10-11 0.0018784414612938788 0.0 4.174314358430842E-5 12-13 0.0028176621919408186 0.0 4.174314358430842E-5 14-15 0.003944727068717145 0.0 4.174314358430842E-5 16-17 0.0050926635172856265 0.0 4.174314358430842E-5 18-19 0.006407572540191342 0.0 1.2522943075292525E-4 20-21 0.00807729828356368 0.0 1.2522943075292525E-4 22-23 0.010227070178155563 0.0 1.2522943075292525E-4 24-25 0.012961246082927765 0.0 1.2522943075292525E-4 26-27 0.015987623992790124 0.0 1.2522943075292525E-4 28-29 0.019076616618028945 0.0 1.4610100254507948E-4 30-31 0.02807226406044741 0.0 1.6697257433723368E-4 32-33 0.0314534586907764 0.0 1.6697257433723368E-4 34-35 0.03489726803648184 0.0 1.6697257433723368E-4 36-37 0.03867502253086175 0.0 1.6697257433723368E-4 38-39 0.04278672217391613 0.0 1.6697257433723368E-4 40-41 0.04673144924263327 0.0 2.087157179215421E-4 42-43 0.05146929603945228 0.0 2.087157179215421E-4 44-45 0.05572709668505174 0.0 2.087157179215421E-4 46-47 0.06081976020233737 0.0 2.087157179215421E-4 48-49 0.06587068057603869 0.0 2.087157179215421E-4 50-51 0.07198605111113987 0.0 2.087157179215421E-4 52-53 0.07952068852810754 0.0 2.295872897136963E-4 54-55 0.08622046307338904 0.0 2.504588615058505E-4 56-57 0.09415166035440764 0.0 2.504588615058505E-4 58-59 0.10222895863797132 0.0 2.504588615058505E-4 60-61 0.11182988166236225 0.0 2.504588615058505E-4 62-63 0.12063768495865132 0.0 2.504588615058505E-4 64-65 0.13055168155992458 0.0 2.504588615058505E-4 66-67 0.14196843133023293 0.0 2.504588615058505E-4 68-69 0.15382348410817653 0.0 2.504588615058505E-4 70-71 0.16757784991920616 0.0 2.504588615058505E-4 72-73 0.18517258493999214 0.0 2.504588615058505E-4 74-75 0.2052092938604602 0.0 2.713304332980047E-4 76-77 0.2254964616424341 0.0 2.922020050901589E-4 78-79 0.24981184278029372 0.0 2.922020050901589E-4 80-81 0.27554649080001986 0.0 2.922020050901589E-4 82-83 0.3059354993293964 0.0 2.922020050901589E-4 84-85 0.34254423625283487 0.0 2.922020050901589E-4 86-87 0.38257591095018667 0.0 2.922020050901589E-4 88-89 0.42728281772898097 0.0 3.7568829225877577E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 3735 0.0 56.337997 3 GATCGGA 3860 0.0 54.725746 1 ATCGGAA 3855 0.0 54.461067 2 CGGAAGA 3870 0.0 54.248848 4 GAAGAGC 4310 0.0 48.811714 6 ATGCCGT 2380 0.0 43.008923 48-49 GCCGTCT 2380 0.0 42.914062 50-51 TGCCGGC 100 6.366463E-10 42.749474 2 CGACGGG 90 1.1805241E-8 42.29056 1 TATGCCG 2420 0.0 42.199894 48-49 TGCCGTC 2385 0.0 42.12696 50-51 CGTATGC 2445 0.0 41.4705 46-47 TCGTATG 2495 0.0 41.210045 44-45 AATCTCG 2410 0.0 41.18599 40-41 CGTCTTC 2475 0.0 41.172173 52-53 CCGTCTT 2485 0.0 40.528564 52-53 CTCGTAT 2450 0.0 40.51314 44-45 TCTCGTA 2485 0.0 40.32476 42-43 CGCGCCC 60 8.295072E-5 39.582016 4 CACCGAG 85 3.596997E-7 39.180965 1 >>END_MODULE