FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395603
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT191110.7977532170397181TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC72400.30222035955039295No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG64210.26803272495484437No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA32890.13729319924879038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG37350.056.3379973
GATCGGA38600.054.7257461
ATCGGAA38550.054.4610672
CGGAAGA38700.054.2488484
GAAGAGC43100.048.8117146
ATGCCGT23800.043.00892348-49
GCCGTCT23800.042.91406250-51
TGCCGGC1006.366463E-1042.7494742
CGACGGG901.1805241E-842.290561
TATGCCG24200.042.19989448-49
TGCCGTC23850.042.1269650-51
CGTATGC24450.041.470546-47
TCGTATG24950.041.21004544-45
AATCTCG24100.041.1859940-41
CGTCTTC24750.041.17217352-53
CCGTCTT24850.040.52856452-53
CTCGTAT24500.040.5131444-45
TCTCGTA24850.040.3247642-43
CGCGCCC608.295072E-539.5820164
CACCGAG853.596997E-739.1809651
Produced by FastQC (version 0.11.2)